FastQCFastQC Report
Wed 25 May 2016
SRR1294615_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294615_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4617026
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC424000.9183400743249008No Hit
GTACATGGGAAGCAGTGGTATCAAC376600.8156765848838624No Hit
GTATCAACGCAGAGTACTTTTTTTT214900.4654511367274085No Hit
GAGTACATGGGAAGCAGTGGTATCA190370.4123216979934703No Hit
CATGTACTCTGCGTTGATACCACTG159910.34634849359739367No Hit
GGTATCAACGCAGAGTACTTTTTTT156850.3397208506081621No Hit
TATCAACGCAGAGTACTTTTTTTTT136890.296489558430037No Hit
GCGTTGATACCACTGCTTCCCATGT127310.2757402709016583No Hit
ACGCAGAGTACATGGGAAGCAGTGG111790.24212555874712424No Hit
GTATCAACGCAGAGTACATGGGAAG105270.22800391420797717No Hit
TATCAACGCAGAGTACATGGGAAGC89040.1928514156082292No Hit
ACTCTGCGTTGATACCACTGCTTCC88700.1921150108316479No Hit
ACGCAGAGTACTTTTTTTTTTTTTT85410.18498921167002308No Hit
GCTTCCCATGTACTCTGCGTTGATA84350.18269336148421084No Hit
GGTATCAACGCAGAGTACATGGGAA77260.16733715599608925No Hit
ATTCCATTCCATTCCATTCCATTCC62300.13493534582651256No Hit
CAGTGGTATCAACGCAGAGTACATG60430.13088511955531548No Hit
GTGGTATCAACGCAGAGTACATGGG57540.12462567895437453No Hit
GAATGGAATGGAATGGAATGGAATG51020.11050403441522746No Hit
CATGGGAAGCAGTGGTATCAACGCA46200.10006441375898685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT951.33023605E-511.0232196
ACCGCGC1752.1827873E-1110.83799618
TATACCG1158.5923784E-710.7617995
CCGTACA4700.010.69387417
CCCCGTA5050.010.32828815
CCCGTAC4900.010.25750116
ACACCGT1303.737241E-710.2523656
CAGGACG1606.43322E-89.5200534
GGACCGT1304.1310377E-69.5200536
GGACCGA800.00444037759.5200536
TAGGACC3000.09.5200534
GTCCCGC5850.09.40229512
GCCCCGT4550.09.37903514
TATTCCG2550.09.3333855
CGCCCTA5800.09.31984216
TCCCGCC6200.09.17733513
CTAGGAC4250.09.1638273
CCCGCCC6350.09.1098914
GTACCGT1151.0863105E-49.1061376
GGTGCGC1054.401944E-49.0667178
Produced by FastQC (version 0.11.5)