##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294615_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4617026 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.249750597029344 31.0 31.0 34.0 28.0 34.0 2 31.46882365401451 31.0 31.0 34.0 28.0 34.0 3 31.5960492750095 31.0 31.0 34.0 28.0 34.0 4 35.20038093785913 37.0 35.0 37.0 32.0 37.0 5 35.310685926395045 37.0 35.0 37.0 32.0 37.0 6 34.87171612202314 37.0 35.0 37.0 32.0 37.0 7 34.72787331065496 37.0 35.0 37.0 32.0 37.0 8 34.76557615226771 37.0 35.0 37.0 32.0 37.0 9 36.25026369788691 39.0 37.0 39.0 31.0 39.0 10 36.052364877304136 39.0 35.0 39.0 30.0 39.0 11 36.17992794495851 39.0 35.0 39.0 31.0 39.0 12 36.13813805683572 39.0 35.0 39.0 30.0 39.0 13 36.00493867697518 39.0 35.0 39.0 30.0 39.0 14 37.10317290827472 40.0 36.0 41.0 31.0 41.0 15 37.13025657641954 40.0 36.0 41.0 31.0 41.0 16 37.158544482963705 40.0 37.0 41.0 31.0 41.0 17 37.20543375757468 40.0 37.0 41.0 31.0 41.0 18 37.21432173004874 40.0 37.0 41.0 31.0 41.0 19 37.217932279350386 40.0 37.0 41.0 31.0 41.0 20 37.288115553172105 40.0 37.0 41.0 31.0 41.0 21 37.1304590877331 40.0 37.0 41.0 30.0 41.0 22 37.145568814210705 40.0 37.0 41.0 30.0 41.0 23 37.15109444911075 40.0 37.0 41.0 30.0 41.0 24 37.07629824913267 40.0 36.0 41.0 30.0 41.0 25 36.00368960452031 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.48579340530895365 1101 2 0.48234993116542313 1101 3 0.3974197393168666 1101 4 0.3341097953077039 1101 5 0.3680961198648731 1101 6 0.4537842388026405 1101 7 0.5686817933208346 1101 8 0.5668064569694451 1101 9 0.7309432411142822 1101 10 0.7653125415189521 1101 11 0.6876874261423538 1101 12 0.7753484686350802 1101 13 0.7436631119416788 1101 14 0.8639573827912912 1101 15 0.85143364157598 1101 16 0.9236667982276572 1101 17 0.9521669284745258 1101 18 0.9197833127334505 1101 19 1.0313829635428249 1101 20 0.9551620176587363 1101 21 1.2023248693694342 1101 22 1.111162001665889 1101 23 1.0904717090430012 1101 24 1.163943126374825 1101 25 1.1354931084091078 1102 1 0.37360411874547594 1102 2 0.4218685646131881 1102 3 0.3792348742963547 1102 4 0.33453219983252325 1102 5 0.3770883569254764 1102 6 0.4536202791515649 1102 7 0.5615383501092808 1102 8 0.5162845130366165 1102 9 0.6630856317406639 1102 10 0.7385081903665949 1102 11 0.7553380334427544 1102 12 0.8134689669617643 1102 13 0.7707183831575364 1102 14 1.0402755639484695 1102 15 0.9838453671134388 1102 16 0.9708731686482537 1102 17 0.8519351237131261 1102 18 0.8868324605556666 1102 19 0.9939738326056187 1102 20 0.9305717879890878 1102 21 1.2078349465623148 1102 22 1.0547286911658489 1102 23 1.0476895336713667 1102 24 1.0903647451216472 1102 25 1.0062545940802323 1103 1 0.2710525737449707 1103 2 0.322854531479674 1103 3 0.24334634440449676 1103 4 0.2234068398894422 1103 5 0.1936925535633378 1103 6 0.2806863206272112 1103 7 0.3182338155522544 1103 8 0.36209568379982926 1103 9 0.2688832777956023 1103 10 0.47982777869111715 1103 11 0.46188555334663306 1103 12 0.5012422258619225 1103 13 0.6698158581170262 1103 14 0.7561957171735543 1103 15 0.7247880786883343 1103 16 0.7769046376195945 1103 17 0.6885648216534435 1103 18 0.6521628263914607 1103 19 0.730048301013916 1103 20 0.7162162251917437 1103 21 0.9641484031787897 1103 22 0.8344334299221003 1103 23 0.8770243402146107 1103 24 0.9850625817339704 1103 25 0.9486181242583456 1104 1 0.13909484127791316 1104 2 0.15579254942106857 1104 3 0.07522657397147015 1104 4 0.08236734289526026 1104 5 0.0878097181398445 1104 6 0.10951220081149415 1104 7 0.18023002088992257 1104 8 0.1439144513106143 1104 9 -0.06314034008342162 1104 10 0.30100124711152887 1104 11 0.38128437693297457 1104 12 0.41018106762751216 1104 13 -0.11093951438928684 1104 14 0.20840458361595182 1104 15 0.37312013065223226 1104 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0.2296460273090979 2207 2 0.29347720291444546 2207 3 0.25763122046564746 2207 4 0.20151972947991226 2207 5 0.20391882986933751 2207 6 0.3303138461049855 2207 7 0.30335399359265836 2207 8 0.3034024359532097 2207 9 0.3782250374130527 2207 10 0.5652628753365221 2207 11 0.5171347498705359 2207 12 0.4272057089907477 2207 13 0.4759117282479721 2207 14 0.512584932331734 2207 15 0.4782709394315461 2207 16 0.5659215932000379 2207 17 0.565086218077056 2207 18 0.5637330740082689 2207 19 0.7171338279127681 2207 20 0.5322711417326644 2207 21 0.8999940092570142 2207 22 0.7607564846697343 2207 23 0.6963991347177085 2207 24 0.8711379534891392 2207 25 0.7980910810580539 2208 1 0.11853615172990573 2208 2 0.15803668361763457 2208 3 0.1625090249858303 2208 4 0.15185913696273445 2208 5 0.1242280848820343 2208 6 0.2603184917050143 2208 7 0.24298405062481265 2208 8 0.16415572329180605 2208 9 0.26757697620973886 2208 10 0.2675896359145966 2208 11 -0.02892759616845808 2208 12 -0.16377518941119007 2208 13 -0.029375009881860592 2208 14 -0.3761409006638843 2208 15 0.14754413228764918 2208 16 0.22821207444246738 2208 17 0.171570811268559 2208 18 0.3270062561564302 2208 19 0.5122203439763311 2208 20 0.34153696532725775 2208 21 0.5977068131566767 2208 22 0.6797396364073336 2208 23 0.5482816460653837 2208 24 0.6857266888425926 2208 25 0.5917222777997679 2209 1 0.022131765537444892 2209 2 0.01980543151520564 2209 3 0.14476623729678906 2209 4 -0.4919402363751999 2209 5 -0.014826371418799056 2209 6 0.21548313917521256 2209 7 0.210599075050105 2209 8 0.22960667955008063 2209 9 0.28729644005184696 2209 10 0.2877673874967357 2209 11 0.3595787118844811 2209 12 0.37774685972733124 2209 13 0.45033168052410133 2209 14 0.4447836239560914 2209 15 -0.13269807268095235 2209 16 -0.07300296611368395 2209 17 0.2813626534951723 2209 18 0.019528437191240755 2209 19 0.31925250718661147 2209 20 0.32904428907340844 2209 21 0.540179018794106 2209 22 0.6223517629566331 2209 23 0.5775142017954735 2209 24 0.7164440043631615 2209 25 0.7222986149259896 2210 1 -0.10557134619506314 2210 2 -0.148525670993795 2210 3 -0.05124774281610556 2210 4 -0.037958430084231054 2210 5 -0.05254258707720538 2210 6 -0.5872844448981382 2210 7 -0.22762517764079604 2210 8 -0.3053109136387988 2210 9 4.756945712571792E-4 2210 10 0.19010433442785057 2210 11 0.10274617504188654 2210 12 0.11661943327032986 2210 13 0.19834368221917487 2210 14 0.19688729863368337 2210 15 0.12905837495954842 2210 16 0.1856448900321297 2210 17 -0.9578780143045122 2210 18 -1.4395326139800986 2210 19 -0.31885374207609374 2210 20 -0.12016013020397054 2210 21 -0.3960273402608294 2210 22 -0.3122922630854248 2210 23 -0.18877350723268194 2210 24 0.19187106537695087 2210 25 0.24930645885380187 2211 1 -0.2075794031707403 2211 2 -0.24775600150947596 2211 3 -0.15386433864562932 2211 4 -0.108802861112828 2211 5 -0.157156050805483 2211 6 -0.09683206707284597 2211 7 -0.21043145215246284 2211 8 -0.19990767225888817 2211 9 -0.12630025628151031 2211 10 -0.06364874498414252 2211 11 -0.39092253721756265 2211 12 -0.24747505055555763 2211 13 -0.19181843383273645 2211 14 -0.26263894353301964 2211 15 -0.35999910754568276 2211 16 -0.28062213176078643 2211 17 -0.4195209498885504 2211 18 -0.4451145360714506 2211 19 -0.26802454216495875 2211 20 -0.3273815947836809 2211 21 -0.05744413188779163 2211 22 -0.17210406499629727 2211 23 -0.631321774666965 2211 24 -0.1294444118653857 2211 25 -0.7780814748461253 2212 1 -0.17277820868711302 2212 2 -0.34993171765178843 2212 3 -0.20115037438489836 2212 4 -0.06730592846134442 2212 5 -0.10464669202903565 2212 6 -0.17372949340354182 2212 7 -0.17033041851449582 2212 8 -0.35144680204640366 2212 9 -0.1097409895786825 2212 10 -0.3888631634870805 2212 11 -0.13842628558263925 2212 12 -0.2724601056402278 2212 13 -0.20880339367680278 2212 14 -0.258749104549743 2212 15 -0.11942399761444733 2212 16 -0.2979197443192305 2212 17 -0.5484177816589622 2212 18 -0.3622199567875768 2212 19 -0.5099246985722274 2212 20 -0.6741473209262523 2212 21 -0.11400167871172329 2212 22 -0.38442426945845654 2212 23 -0.33641189955698536 2212 24 -0.49441924014333694 2212 25 -0.26993551353322687 2213 1 -0.17073983159090744 2213 2 -0.25025127892708454 2213 3 -0.14634497177497607 2213 4 -0.046748871198950326 2213 5 -0.09738669113027498 2213 6 -0.3046499066051567 2213 7 -0.3899492780988183 2213 8 -0.35459435155935637 2213 9 -0.3776353325676638 2213 10 -0.33346179286571953 2213 11 -0.07602753764081172 2213 12 0.038499816406798004 2213 13 0.013130018044876124 2213 14 -0.7008572552454595 2213 15 -0.17522442244607106 2213 16 -0.39618625089004667 2213 17 -0.34206052371637696 2213 18 -0.2785440381627353 2213 19 -0.3408913214539311 2213 20 -0.3959206475738384 2213 21 -0.033292587506771554 2213 22 -0.38414844465392406 2213 23 -0.5091253474898991 2213 24 -0.6667121886086633 2213 25 -0.45294521745827865 2214 1 -0.2035420741867 2214 2 -0.07592203673990738 2214 3 -0.11395872063342694 2214 4 -0.004125461139906861 2214 5 -0.11214094632654081 2214 6 -0.09568257199460106 2214 7 -0.10992655681741326 2214 8 -0.11716516433515523 2214 9 -0.15842923161094546 2214 10 -0.08325072085269625 2214 11 0.004370390749457442 2214 12 -0.22414039294471166 2214 13 -0.29174066819857103 2214 14 -0.17385239815030218 2214 15 -0.02972640889533551 2214 16 -0.24519155185722497 2214 17 -0.30300294473454414 2214 18 -0.2001193116085247 2214 19 -0.4548613399160786 2214 20 -0.39960759008708635 2214 21 -0.5326690714682911 2214 22 -0.7561419710398098 2214 23 -0.9800603765533182 2214 24 -0.6327973282299197 2214 25 -0.7312572466341081 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 10.0 9 52.0 10 119.0 11 313.0 12 791.0 13 1915.0 14 3899.0 15 6901.0 16 10347.0 17 13267.0 18 15700.0 19 17598.0 20 19214.0 21 21068.0 22 22889.0 23 25279.0 24 28286.0 25 32168.0 26 36839.0 27 41488.0 28 48828.0 29 60508.0 30 78327.0 31 101714.0 32 136220.0 33 184652.0 34 253790.0 35 354287.0 36 506421.0 37 805755.0 38 1788379.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.513244772519194 21.381294608410755 12.200197766980546 24.905262852089507 2 14.154739436165183 22.25974036100295 39.20320136815344 24.382318834678426 3 18.228463970293383 27.525965856560425 29.82785142960499 24.417718743541204 4 11.928924278969824 18.017084426787914 38.2307968075278 31.823194486714456 5 12.806384203874421 38.24787944934357 35.30904775501308 13.636688591768923 6 30.688236763307092 38.43862012554829 17.513811113486703 13.359331997657911 7 24.896999254330492 33.62601186398745 23.87931852479622 17.597670356885832 8 24.124013509210133 37.612600219665474 21.51014598872211 16.75324028240228 9 24.56491948592804 16.428349114439424 22.285678269896657 36.72105312973588 10 14.74860038818858 30.340740167385942 33.67408727089775 21.236572173527733 11 31.87033824193949 26.202138922692974 22.51820273276687 19.40932010260067 12 20.819206065985437 28.234239947601864 32.01945956623387 18.92709442017883 13 26.976375479881526 23.58033582230982 26.835960385746233 22.60732831206242 14 20.101642176998347 23.39112939819332 28.768799351784207 27.738429073024122 15 24.247569945163217 29.199016498685072 24.306071465856206 22.247342090295504 16 20.872221176573532 30.907380241398286 27.22151553697983 20.99888304504835 17 20.287349537851757 30.192667356143836 29.273807267666953 20.246175838337457 18 20.661024922612288 29.08649576825878 30.217833525232336 20.0346457838966 19 20.62298610631301 28.915425182949324 30.700831212723273 19.760757498014396 20 22.017636062418656 27.771567659378338 29.594584555352515 20.61621172285049 21 21.546249450673454 29.105329263461666 29.055231982035153 20.293189303829724 22 20.56168044948298 28.723353101458564 29.196878397872144 21.518088051186304 23 19.908897966892507 29.160599442800887 29.754705469175903 21.175797121130703 24 20.459754928769065 29.040648469523124 29.31112567075119 21.188470930956623 25 21.39474580221254 27.72924411668641 28.162906469110816 22.71310361199024 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3313.0 1 3313.0 2 3646.5 3 3980.0 4 3980.0 5 3980.0 6 7171.0 7 10362.0 8 10362.0 9 10362.0 10 14900.0 11 19438.0 12 19438.0 13 19438.0 14 29466.5 15 39495.0 16 39495.0 17 39495.0 18 64215.0 19 88935.0 20 88935.0 21 88935.0 22 130415.5 23 171896.0 24 171896.0 25 171896.0 26 225401.5 27 278907.0 28 278907.0 29 278907.0 30 332682.0 31 386457.0 32 386457.0 33 386457.0 34 431471.5 35 476486.0 36 476486.0 37 476486.0 38 502886.0 39 529286.0 40 529286.0 41 529286.0 42 531000.0 43 532714.0 44 532714.0 45 532714.0 46 593147.5 47 653581.0 48 653581.0 49 653581.0 50 590489.5 51 527398.0 52 527398.0 53 527398.0 54 441369.0 55 355340.0 56 355340.0 57 355340.0 58 296026.5 59 236713.0 60 236713.0 61 236713.0 62 197800.5 63 158888.0 64 158888.0 65 158888.0 66 120470.0 67 82052.0 68 82052.0 69 82052.0 70 60458.5 71 38865.0 72 38865.0 73 38865.0 74 27169.5 75 15474.0 76 15474.0 77 15474.0 78 10377.0 79 5280.0 80 5280.0 81 5280.0 82 3430.0 83 1580.0 84 1580.0 85 1580.0 86 1015.5 87 451.0 88 451.0 89 451.0 90 279.0 91 107.0 92 107.0 93 107.0 94 65.0 95 23.0 96 23.0 97 23.0 98 14.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09805013010539686 2 0.0 3 0.1314049346917258 4 0.011717499533249326 5 0.021832235729233495 6 0.013753442150856417 7 0.02252532257778059 8 0.019839611039660598 9 1.5161274811967705E-4 10 0.3836885475628684 11 0.0654317302956492 12 0.001472809553162577 13 2.165896401709672E-5 14 9.746533807693525E-4 15 0.0011262661288890296 16 1.9493067615387048E-4 17 2.165896401709672E-5 18 3.032254962393541E-4 19 8.013816686325785E-4 20 1.9493067615387048E-4 21 1.9493067615387048E-4 22 0.0035953880268380554 23 1.082948200854836E-4 24 2.165896401709672E-5 25 0.0015161274811967703 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4617026.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.83092113197921 #Duplication Level Percentage of deduplicated Percentage of total 1 82.92653430466979 54.59130139559084 2 11.913613401626574 15.685682884787388 3 2.858074434316678 5.644490180244819 4 0.9660507950235897 2.5438405478673496 5 0.4123279381595266 1.3571963988745697 6 0.23041352677755791 0.9101000825420755 7 0.1341892098828309 0.6183659504791472 8 0.09214749509144265 0.4852923585499362 9 0.066566843608422 0.394394096843174 >10 0.34845790705219154 4.114627405871835 >50 0.026857010114952666 1.2303951335127585 >100 0.019706500622423403 2.531703544080148 >500 0.002414550792781796 1.1137751311936606 >1k 0.002017637724295963 2.9870139492409162 >5k 2.9768425440419903E-4 1.4284999390579824 >10k+ 3.3076028267133223E-4 4.363321001263472 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 42400 0.9183400743249008 No Hit GTACATGGGAAGCAGTGGTATCAAC 37660 0.8156765848838624 No Hit GTATCAACGCAGAGTACTTTTTTTT 21490 0.4654511367274085 No Hit GAGTACATGGGAAGCAGTGGTATCA 19037 0.4123216979934703 No Hit CATGTACTCTGCGTTGATACCACTG 15991 0.34634849359739367 No Hit GGTATCAACGCAGAGTACTTTTTTT 15685 0.3397208506081621 No Hit TATCAACGCAGAGTACTTTTTTTTT 13689 0.296489558430037 No Hit GCGTTGATACCACTGCTTCCCATGT 12731 0.2757402709016583 No Hit ACGCAGAGTACATGGGAAGCAGTGG 11179 0.24212555874712424 No Hit GTATCAACGCAGAGTACATGGGAAG 10527 0.22800391420797717 No Hit TATCAACGCAGAGTACATGGGAAGC 8904 0.1928514156082292 No Hit ACTCTGCGTTGATACCACTGCTTCC 8870 0.1921150108316479 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 8541 0.18498921167002308 No Hit GCTTCCCATGTACTCTGCGTTGATA 8435 0.18269336148421084 No Hit GGTATCAACGCAGAGTACATGGGAA 7726 0.16733715599608925 No Hit ATTCCATTCCATTCCATTCCATTCC 6230 0.13493534582651256 No Hit CAGTGGTATCAACGCAGAGTACATG 6043 0.13088511955531548 No Hit GTGGTATCAACGCAGAGTACATGGG 5754 0.12462567895437453 No Hit GAATGGAATGGAATGGAATGGAATG 5102 0.11050403441522746 No Hit CATGGGAAGCAGTGGTATCAACGCA 4620 0.10006441375898685 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 8.663585606838687E-5 0.0 4 0.0 0.0 0.0 8.663585606838687E-5 0.0 5 0.0 0.0 0.0 1.2995378410258032E-4 0.0 6 0.0 0.0 0.0 1.5161274811967705E-4 0.0 7 0.0 0.0 0.0 1.5161274811967705E-4 0.0 8 0.0 0.0 0.0 1.9493067615387048E-4 0.0 9 0.0 0.0 0.0 2.5990756820516063E-4 0.0 10 0.0 0.0 0.0 2.5990756820516063E-4 0.0 11 0.0 0.0 0.0 2.8156653222225733E-4 0.0 12 0.0 0.0 0.0 3.032254962393541E-4 0.0 13 0.0 0.0 0.0 3.465434242735475E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCGT 95 1.33023605E-5 11.023219 6 ACCGCGC 175 2.1827873E-11 10.837996 18 TATACCG 115 8.5923784E-7 10.761799 5 CCGTACA 470 0.0 10.693874 17 CCCCGTA 505 0.0 10.328288 15 CCCGTAC 490 0.0 10.257501 16 ACACCGT 130 3.737241E-7 10.252365 6 CAGGACG 160 6.43322E-8 9.520053 4 GGACCGT 130 4.1310377E-6 9.520053 6 GGACCGA 80 0.0044403775 9.520053 6 TAGGACC 300 0.0 9.520053 4 GTCCCGC 585 0.0 9.402295 12 GCCCCGT 455 0.0 9.379035 14 TATTCCG 255 0.0 9.333385 5 CGCCCTA 580 0.0 9.319842 16 TCCCGCC 620 0.0 9.177335 13 CTAGGAC 425 0.0 9.163827 3 CCCGCCC 635 0.0 9.10989 14 GTACCGT 115 1.0863105E-4 9.106137 6 GGTGCGC 105 4.401944E-4 9.066717 8 >>END_MODULE