FastQCFastQC Report
Wed 25 May 2016
SRR1294614_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294614_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3786872
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC286360.7561913896218303No Hit
CCCATGTACTCTGCGTTGATACCAC266420.7035357941858082No Hit
GAGTACATGGGAAGCAGTGGTATCA130840.345509433643387No Hit
CATGTACTCTGCGTTGATACCACTG99270.2621424753728143No Hit
GCGTTGATACCACTGCTTCCCATGT92770.24497791316949713No Hit
GTATCAACGCAGAGTACTTTTTTTT81460.21511157493572533No Hit
GTATCAACGCAGAGTACATGGGAAG77930.20578989730838537No Hit
ATTCCATTCCATTCCATTCCATTCC69460.18342315240652443No Hit
ACGCAGAGTACATGGGAAGCAGTGG66920.17671576963784358No Hit
TATCAACGCAGAGTACATGGGAAGC62860.16599452001546394No Hit
GCTTCCCATGTACTCTGCGTTGATA61840.16330100410048187No Hit
GGTATCAACGCAGAGTACATGGGAA58570.15466590896127463No Hit
ACTCTGCGTTGATACCACTGCTTCC55470.14647973314123106No Hit
GGTATCAACGCAGAGTACTTTTTTT54400.14365418213237732No Hit
GAATGGAATGGAATGGAATGGAATG52940.13979875739132455No Hit
TATCAACGCAGAGTACTTTTTTTTT47090.12435065140833912No Hit
GTGGTATCAACGCAGAGTACATGGG41480.10953631387593771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG1052.0030711E-812.66384719
CCGGCAC600.00589152411.0799883
CCCCGTA4000.010.93080315
AAATCGC1158.764182E-710.74511817
CCGTTCA1158.8722845E-710.7348969
TGTCCCG3550.010.43250411
CCCGTAC4200.010.41042716
CCGTACA4250.010.28808817
TGGACCG750.002656880510.1297395
CCGTGCA856.621568E-410.0548579
CCCGCCC4100.09.96870614
GTACTGG10150.09.6402861
CGCCCTA4050.09.6225216
TATTCCG2201.6370905E-119.496635
GTATTAG5750.09.4172861
TAGGACT5150.09.4044294
CTAGGAC3550.09.363373
TAGACAG5500.09.3239645
GTCCCGC4200.09.2784912
GTCCTAG3350.09.0745261
Produced by FastQC (version 0.11.5)