##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294614_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3786872 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.963677673816278 31.0 30.0 33.0 25.0 34.0 2 30.213888401826097 31.0 30.0 34.0 25.0 34.0 3 30.16570826793195 31.0 30.0 34.0 25.0 34.0 4 33.813422793271066 35.0 35.0 37.0 28.0 37.0 5 34.13071843991558 35.0 35.0 37.0 30.0 37.0 6 34.187998960619744 37.0 35.0 37.0 30.0 37.0 7 34.098257083946855 37.0 35.0 37.0 29.0 37.0 8 33.9530382859521 37.0 35.0 37.0 28.0 37.0 9 35.14246824291922 39.0 35.0 39.0 27.0 39.0 10 35.24732708155966 39.0 35.0 39.0 28.0 39.0 11 35.472731848343436 39.0 35.0 39.0 28.0 39.0 12 35.265857942914366 39.0 35.0 39.0 27.0 39.0 13 35.12324921465526 38.0 35.0 39.0 27.0 39.0 14 36.03329502555143 39.0 35.0 41.0 27.0 41.0 15 35.997211946957805 39.0 35.0 41.0 27.0 41.0 16 36.1038514108742 39.0 35.0 41.0 27.0 41.0 17 36.094697945956455 39.0 35.0 41.0 27.0 41.0 18 35.980600083657436 39.0 35.0 41.0 27.0 41.0 19 36.246038154973284 39.0 35.0 41.0 27.0 41.0 20 36.36495582633899 39.0 36.0 41.0 27.0 41.0 21 36.398134925078004 39.0 36.0 41.0 27.0 41.0 22 36.40470551948943 39.0 36.0 41.0 27.0 41.0 23 36.38000201749623 39.0 36.0 41.0 27.0 41.0 24 36.39097967927091 39.0 36.0 41.0 27.0 41.0 25 35.145724228334096 38.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9611412411877112 1101 2 0.9667904684996422 1101 3 1.2499586967912606 1101 4 1.1041682763273144 1101 5 0.7406339380068587 1101 6 0.7315868250569508 1101 7 0.7901315914228704 1101 8 0.9042409598666552 1101 9 1.1310301782040213 1101 10 1.1052096036653367 1101 11 0.9165699216583789 1101 12 1.1224499495262492 1101 13 1.1763708423721368 1101 14 1.2588101093278894 1101 15 1.4770158159394455 1101 16 1.3334083127884284 1101 17 1.2907285582715957 1101 18 1.2838916782292813 1101 19 1.1728858568238039 1101 20 1.0613922811467873 1101 21 0.9978257693849528 1101 22 0.948152660174614 1101 23 0.9393236423919902 1101 24 1.0788885576665805 1101 25 0.9745671585044491 1102 1 0.8405167672031801 1102 2 0.844883734371269 1102 3 1.1609087466263937 1102 4 0.9834993717439175 1102 5 0.8193948352595157 1102 6 0.6958281367884851 1102 7 0.693502836614968 1102 8 0.816921173379697 1102 9 1.1756725209226673 1102 10 1.058292187840273 1102 11 0.8258560976116911 1102 12 1.0485707752984226 1102 13 0.9729305626576874 1102 14 1.3326953281624725 1102 15 1.4243535949014685 1102 16 1.1979310347482652 1102 17 1.2847739084176766 1102 18 1.2967710186198786 1102 19 1.069798795804921 1102 20 0.39552634530168973 1102 21 0.6281629859428932 1102 22 0.6812141152531694 1102 23 0.809314761202863 1102 24 0.9214710737164253 1102 25 0.9444098666971854 1103 1 0.6317659508705269 1103 2 0.5867509625851426 1103 3 0.9266299575904 1103 4 0.6995837519067223 1103 5 0.556356844515939 1103 6 0.4381208592318302 1103 7 0.36084936792349964 1103 8 0.5597906305013041 1103 9 0.6529811148383473 1103 10 0.6215533132036981 1103 11 0.5994447491579891 1103 12 0.7898399691836886 1103 13 0.6616203781218033 1103 14 0.5481461868949111 1103 15 0.867716162020308 1103 16 0.8092762408601573 1103 17 0.8345410494821763 1103 18 0.9093366965443366 1103 19 0.7076467522872747 1103 20 -0.1682258543074795 1103 21 0.22708966460287883 1103 22 -0.22989577498177027 1103 23 0.300093361146736 1103 24 0.4135765698316547 1103 25 0.369608228696805 1104 1 0.2793455429256433 1104 2 0.35056989076080214 1104 3 0.641860444669831 1104 4 0.5497268752469395 1104 5 0.38486898868643493 1104 6 0.28189290301167347 1104 7 0.30066745120549143 1104 8 0.39683505415167986 1104 9 0.6760363454253806 1104 10 0.6573877074312549 1104 11 0.3783098730852785 1104 12 0.5097749360368979 1104 13 0.41849572235202714 1104 14 0.7171391039285027 1104 15 0.7305181200040423 1104 16 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0.41279149458316056 2207 7 0.35046136023076 2207 8 0.349460321201164 2207 9 -1.2957820952079189 2207 10 -0.21658058669081015 2207 11 0.19240441110483886 2207 12 0.5318290232860932 2207 13 0.6705266612312641 2207 14 0.8616279137752159 2207 15 0.8995817108398398 2207 16 0.8438561552155832 2207 17 0.7647605933734383 2207 18 0.8679509129459007 2207 19 -0.29773496155740986 2207 20 0.24321206601217682 2207 21 -0.2131505783534351 2207 22 0.24314318313370364 2207 23 -0.5098077347063921 2207 24 -1.5704972955959988 2207 25 -0.15753600091839104 2208 1 0.4801543507681707 2208 2 0.20684569174620648 2208 3 0.6009949970696162 2208 4 0.5054285861752987 2208 5 0.3833845237945184 2208 6 0.30139452166176994 2208 7 0.3700334065356543 2208 8 0.584830237871472 2208 9 -0.21400366754838984 2208 10 0.04673547595302807 2208 11 0.2730407762144793 2208 12 0.31775309338387814 2208 13 0.4351128447206918 2208 14 0.7587460226029208 2208 15 0.697025796495403 2208 16 0.4385347765882841 2208 17 0.4863649356010029 2208 18 0.6422116775867508 2208 19 -1.445103648211628 2208 20 -0.21673199656520126 2208 21 -0.3717005440643817 2208 22 0.07574606620442381 2208 23 0.24502899173585746 2208 24 0.3897863753250519 2208 25 0.469161502308971 2209 1 0.3133559821177663 2209 2 -1.2596828782469593 2209 3 -0.1207153290730254 2209 4 0.1354076755568272 2209 5 0.10082497603625029 2209 6 0.2089416033531748 2209 7 0.19223704048125967 2209 8 0.38127041137425266 2209 9 -2.041791690517684 2209 10 -0.41977861099645253 2209 11 -0.08105112067422482 2209 12 0.25361285567350933 2209 13 0.4569838253033467 2209 14 0.6677746685468691 2209 15 0.8111767678450263 2209 16 0.7094537228136133 2209 17 0.4760806625899079 2209 18 0.668320260441206 2209 19 0.39700931472416556 2209 20 0.33784315348637506 2209 21 0.4698558610608359 2209 22 0.3391584168090631 2209 23 0.41596121294864474 2209 24 0.44357861463210213 2209 25 0.5744201063401846 2210 1 0.19197680431947362 2210 2 0.1839189503799865 2210 3 0.13335806182837473 2210 4 0.21572760763486087 2210 5 0.1380555508789456 2210 6 0.12940099640354674 2210 7 0.17421918797080593 2210 8 0.21526618589639668 2210 9 0.41409922078292993 2210 10 -1.331629592182125 2210 11 -0.4059184418316377 2210 12 -2.2460121893018155 2210 13 -0.7234063453077795 2210 14 -0.4401847779339221 2210 15 -0.6710601969263692 2210 16 0.09167463839685297 2210 17 0.06074374155904394 2210 18 0.4031397984950047 2210 19 0.29647612890587993 2210 20 0.2960573588026847 2210 21 0.3667488732715327 2210 22 0.2786176828651534 2210 23 0.17682230780963692 2210 24 0.18620825744947211 2210 25 0.06654158447547331 2211 1 -0.30545413436016133 2211 2 -0.10597654830368342 2211 3 -1.5047787290022399 2211 4 -0.5365499255553559 2211 5 -0.43160269857552436 2211 6 -0.4335500681101365 2211 7 -0.43552224078793245 2211 8 -0.05108550376894527 2211 9 0.11986460419345946 2211 10 0.10708171678763279 2211 11 -0.22176571597221795 2211 12 -0.32794563768813134 2211 13 -0.19176606280336017 2211 14 -0.7340884799423648 2211 15 -0.41807250610957425 2211 16 -0.15154927925580353 2211 17 -0.21302897838782542 2211 18 0.024560042396913673 2211 19 -0.04494135780582553 2211 20 -0.09757457438414008 2211 21 -0.16569002578737724 2211 22 -0.20964166115093974 2211 23 -0.5746035944691172 2211 24 -0.3049603695561345 2211 25 -0.23621643938516712 2212 1 -0.377070336445108 2212 2 -0.35741284629318315 2212 3 -2.322345309560024 2212 4 -1.327480936643333 2212 5 -0.6438383019016314 2212 6 -0.4529661654922137 2212 7 -0.3330697908006215 2212 8 -0.42774608273979453 2212 9 -0.8185543937777666 2212 10 -0.22613106090528845 2212 11 -0.295499700403532 2212 12 0.011066855520468266 2212 13 -0.4091922847752798 2212 14 -0.00556163433396506 2212 15 0.07317651297736205 2212 16 -1.0180472361553043 2212 17 -1.2757607260507982 2212 18 -0.31708533149463136 2212 19 -0.1117514114769591 2212 20 -0.19356368842822747 2212 21 -0.10256761613226928 2212 22 -0.2890200861811678 2212 23 -0.1134089382346346 2212 24 -0.16645758845393033 2212 25 -0.06811121376804863 2213 1 -3.0620414754756773 2213 2 -1.338569924045121 2213 3 -2.2469809649630044 2213 4 -0.9893166047988515 2213 5 -0.7963913639051867 2213 6 -0.6217161227194552 2213 7 -0.38325984371865474 2213 8 -0.21431612164672487 2213 9 -0.28431528230269976 2213 10 -0.14083863796761165 2213 11 -0.4552610776645807 2213 12 -0.1643645817960362 2213 13 -0.6141285362593862 2213 14 -0.16230584223509936 2213 15 -0.27440763167518156 2213 16 -1.625939531600963 2213 17 -0.8888730503966471 2213 18 -0.3038092521731599 2213 19 -0.13977628827890243 2213 20 -0.22678944028147896 2213 21 -0.017320511451437426 2213 22 0.010249830858882092 2213 23 0.02755078727192739 2213 24 0.06628621250094113 2213 25 0.04584329571565604 2214 1 -0.9102232602835016 2214 2 -0.8278179078387105 2214 3 -6.717067854156337 2214 4 -2.2222555687699597 2214 5 -1.340352840303936 2214 6 -0.7997027197234772 2214 7 -0.6172800416598108 2214 8 -0.4994883898601685 2214 9 -0.21459208620879622 2214 10 -0.23808303071944437 2214 11 -1.0371747091055568 2214 12 -0.6765903935767241 2214 13 -5.212672595771895 2214 14 -3.841990174313011 2214 15 -2.2938156938309078 2214 16 -2.9990239894988306 2214 17 -3.334181668062506 2214 18 -1.2924716058073358 2214 19 -0.8806505867992698 2214 20 -0.5033953551544315 2214 21 -0.4905396334150254 2214 22 -0.38313494292384576 2214 23 -0.45137003125864794 2214 24 -0.2956198764173905 2214 25 -0.11956364173647671 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1460.0 3 195.0 4 201.0 5 409.0 6 62.0 7 60.0 8 114.0 9 341.0 10 874.0 11 2142.0 12 4342.0 13 7840.0 14 12274.0 15 17403.0 16 21330.0 17 24132.0 18 25717.0 19 26104.0 20 26986.0 21 27561.0 22 28652.0 23 29999.0 24 32080.0 25 34625.0 26 38428.0 27 44041.0 28 50800.0 29 61833.0 30 76238.0 31 95199.0 32 124049.0 33 163955.0 34 219639.0 35 298529.0 36 418323.0 37 631292.0 38 1239643.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.56004618449267 22.006572408572044 13.211376526266372 22.222004880668912 2 13.79183978123163 22.280501740503194 41.620418381299004 22.307240096966176 3 17.834095172668903 29.30752767722673 29.86057019050387 22.997806959600496 4 11.48391474107333 18.057414553749723 39.67976381901316 30.77890688616379 5 12.465201315196758 39.030749131106205 35.36225374737284 13.1417958063242 6 29.475735359403345 39.42702850112254 18.24940173433622 12.847834405137897 7 23.970958833597003 34.131165639340864 25.122046410798376 16.775829116263758 8 23.033523792612886 38.57283957663502 22.43680599154031 15.956830639211784 9 24.133220165565994 17.329281208513372 22.963587953763287 35.573910672157346 10 14.586568128080431 31.176137018242393 33.67219184583334 20.565103007843838 11 30.989049781455392 27.807192445559377 22.839563259562087 18.364194513423147 12 19.86923673071745 29.375838442044216 32.866188282936754 17.88873654430158 13 25.69045028924385 25.313441086234644 27.345824031192308 21.6502845933292 14 19.353921539612813 24.37692102804331 29.294197913980224 26.974959518363654 15 23.594865783587927 30.479111791341996 24.391729487022292 21.534292938047784 16 20.410218360527 32.38037257555184 27.194352997380644 20.01505606654052 17 19.554206507877872 31.365596324699464 29.73897037256652 19.34122679485614 18 19.891577416369167 30.573529022874517 30.409255586407507 19.125637974348802 19 20.05071078337458 29.781658234688656 30.761916968028952 19.40571401390781 20 21.875963646020367 28.879217747337467 29.21440471613482 20.03041389050735 21 21.576144125804888 30.201290676483485 28.833854270295866 19.388710927415765 22 20.029484657962907 29.812958187436067 29.633957405001293 20.523599749599732 23 19.479980350691015 30.540961119682947 29.73025536438555 20.24880316524049 24 19.995000001321355 29.718411808030705 29.421292436233497 20.865295754414444 25 20.977377426154707 28.716153277193317 28.181592604464477 22.1248766921875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4645.0 1 4645.0 2 4863.0 3 5081.0 4 5081.0 5 5081.0 6 7431.0 7 9781.0 8 9781.0 9 9781.0 10 15015.5 11 20250.0 12 20250.0 13 20250.0 14 33047.0 15 45844.0 16 45844.0 17 45844.0 18 69428.0 19 93012.0 20 93012.0 21 93012.0 22 128359.0 23 163706.0 24 163706.0 25 163706.0 26 208871.0 27 254036.0 28 254036.0 29 254036.0 30 300372.5 31 346709.0 32 346709.0 33 346709.0 34 385551.0 35 424393.0 36 424393.0 37 424393.0 38 446860.5 39 469328.0 40 469328.0 41 469328.0 42 459505.0 43 449682.0 44 449682.0 45 449682.0 46 478045.0 47 506408.0 48 506408.0 49 506408.0 50 447643.5 51 388879.0 52 388879.0 53 388879.0 54 320836.5 55 252794.0 56 252794.0 57 252794.0 58 208039.0 59 163284.0 60 163284.0 61 163284.0 62 133270.0 63 103256.0 64 103256.0 65 103256.0 66 76911.5 67 50567.0 68 50567.0 69 50567.0 70 36580.0 71 22593.0 72 22593.0 73 22593.0 74 15621.5 75 8650.0 76 8650.0 77 8650.0 78 5747.0 79 2844.0 80 2844.0 81 2844.0 82 1815.0 83 786.0 84 786.0 85 786.0 86 511.0 87 236.0 88 236.0 89 236.0 90 152.5 91 69.0 92 69.0 93 69.0 94 43.5 95 18.0 96 18.0 97 18.0 98 19.5 99 21.0 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09992415904208012 2 0.05405516743106184 3 0.05735604477785359 4 0.05223308313563278 5 0.04983004442716838 6 0.06865824881326858 7 0.0647236030158928 8 0.06168679585684438 9 0.06654568731132185 10 0.06601754693583517 11 0.06047207299322502 12 0.06134350461277804 13 0.06203008710091072 14 0.06443312580937513 15 0.0669681996117112 16 0.06208290113845939 17 0.06533096444770248 18 0.14185850485572263 19 0.07042751907114896 20 0.06382576437756544 21 0.06192445902581339 22 0.06683616451783952 23 0.06641365221745019 24 0.07581455090111311 25 0.06979375062056493 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3786872.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.58240815279223 #Duplication Level Percentage of deduplicated Percentage of total 1 86.53061602211743 61.940698738077536 2 9.80329321093331 14.034866717330507 3 2.0486825173181664 4.399488843904765 4 0.649658042488958 1.8601634862875454 5 0.27151231560667594 0.9717752697133409 6 0.16151872874862616 0.6937139739362578 7 0.10009479958734147 0.5015518758623111 8 0.07145182045414115 0.40917547000066884 9 0.04749215619441855 0.3059642617888526 >10 0.26969639847200155 3.590488752138095 >50 0.022833271761853163 1.151840206685137 >100 0.018826511809519806 2.683019814730858 >500 0.0020502694045861376 1.0191504875080086 >1k 0.00171477073348275 2.3887448173091435 >5k 4.473314956911514E-4 2.2251705400037083 >10k+ 1.1183287392278785E-4 1.8241867447233269 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 28636 0.7561913896218303 No Hit CCCATGTACTCTGCGTTGATACCAC 26642 0.7035357941858082 No Hit GAGTACATGGGAAGCAGTGGTATCA 13084 0.345509433643387 No Hit CATGTACTCTGCGTTGATACCACTG 9927 0.2621424753728143 No Hit GCGTTGATACCACTGCTTCCCATGT 9277 0.24497791316949713 No Hit GTATCAACGCAGAGTACTTTTTTTT 8146 0.21511157493572533 No Hit GTATCAACGCAGAGTACATGGGAAG 7793 0.20578989730838537 No Hit ATTCCATTCCATTCCATTCCATTCC 6946 0.18342315240652443 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6692 0.17671576963784358 No Hit TATCAACGCAGAGTACATGGGAAGC 6286 0.16599452001546394 No Hit GCTTCCCATGTACTCTGCGTTGATA 6184 0.16330100410048187 No Hit GGTATCAACGCAGAGTACATGGGAA 5857 0.15466590896127463 No Hit ACTCTGCGTTGATACCACTGCTTCC 5547 0.14647973314123106 No Hit GGTATCAACGCAGAGTACTTTTTTT 5440 0.14365418213237732 No Hit GAATGGAATGGAATGGAATGGAATG 5294 0.13979875739132455 No Hit TATCAACGCAGAGTACTTTTTTTTT 4709 0.12435065140833912 No Hit GTGGTATCAACGCAGAGTACATGGG 4148 0.10953631387593771 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 2.6407018774334067E-5 0.0 0.0 0.0 5 0.0 2.6407018774334067E-5 0.0 0.0 0.0 6 0.0 2.6407018774334067E-5 0.0 2.6407018774334067E-5 0.0 7 0.0 2.6407018774334067E-5 0.0 5.2814037548668133E-5 0.0 8 0.0 2.6407018774334067E-5 0.0 5.2814037548668133E-5 0.0 9 0.0 2.6407018774334067E-5 0.0 7.92210563230022E-5 0.0 10 0.0 2.6407018774334067E-5 0.0 1.0562807509733627E-4 0.0 11 0.0 2.6407018774334067E-5 0.0 1.0562807509733627E-4 0.0 12 0.0 2.6407018774334067E-5 0.0 1.3203509387167033E-4 0.0 13 0.0 2.6407018774334067E-5 0.0 1.3203509387167033E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGCCG 105 2.0030711E-8 12.663847 19 CCGGCAC 60 0.005891524 11.079988 3 CCCCGTA 400 0.0 10.930803 15 AAATCGC 115 8.764182E-7 10.745118 17 CCGTTCA 115 8.8722845E-7 10.734896 9 TGTCCCG 355 0.0 10.432504 11 CCCGTAC 420 0.0 10.410427 16 CCGTACA 425 0.0 10.288088 17 TGGACCG 75 0.0026568805 10.129739 5 CCGTGCA 85 6.621568E-4 10.054857 9 CCCGCCC 410 0.0 9.968706 14 GTACTGG 1015 0.0 9.640286 1 CGCCCTA 405 0.0 9.62252 16 TATTCCG 220 1.6370905E-11 9.49663 5 GTATTAG 575 0.0 9.417286 1 TAGGACT 515 0.0 9.404429 4 CTAGGAC 355 0.0 9.36337 3 TAGACAG 550 0.0 9.323964 5 GTCCCGC 420 0.0 9.27849 12 GTCCTAG 335 0.0 9.074526 1 >>END_MODULE