FastQCFastQC Report
Wed 25 May 2016
SRR1294614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294614_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3786872
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC305860.8076850762317819No Hit
GTACATGGGAAGCAGTGGTATCAAC282510.7460246873937117No Hit
GTATCAACGCAGAGTACTTTTTTTT180840.4775445275150573No Hit
GAGTACATGGGAAGCAGTGGTATCA142330.37585109821509677No Hit
GGTATCAACGCAGAGTACTTTTTTT141480.3736065016192784No Hit
TATCAACGCAGAGTACTTTTTTTTT120490.3181781692119512No Hit
CATGTACTCTGCGTTGATACCACTG113270.29911230165688196No Hit
GCGTTGATACCACTGCTTCCCATGT92330.24381600434342643No Hit
ACGCAGAGTACATGGGAAGCAGTGG85140.22482935784468022No Hit
GTATCAACGCAGAGTACATGGGAAG78840.2081929360168498No Hit
ACGCAGAGTACTTTTTTTTTTTTTT74140.1957816371929128No Hit
TATCAACGCAGAGTACATGGGAAGC68830.18175951022374137No Hit
ATTCCATTCCATTCCATTCCATTCC67650.17864348200836996No Hit
ACTCTGCGTTGATACCACTGCTTCC65560.17312441508453416No Hit
GCTTCCCATGTACTCTGCGTTGATA65020.1716984360707201No Hit
GGTATCAACGCAGAGTACATGGGAA62990.1663378112595303No Hit
GAATGGAATGGAATGGAATGGAATG55590.14679661736652308No Hit
GTGGTATCAACGCAGAGTACATGGG43840.11576837030668055No Hit
CAGTGGTATCAACGCAGAGTACATG43530.11494975272467621No Hit
GTACTTTTTTTTTTTTTTTTTTTTT38790.10243282582564185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC400.00521114614.2786110
TGGACCG551.9267153E-413.847215
ATCGCCG604.1576926E-412.64366519
GGACCGT853.8559956E-612.3199456
GCGCAGC658.114486E-411.67739711
TATACCG1156.881055E-811.5895135
TAGTCCG855.2146337E-511.199955
CGCCCTA3600.011.06291516
TGTCCCG3600.010.54209511
CTAGTAC2600.010.2327283
AGGACCG750.002603350710.1546225
CCGCCCT4100.09.94479715
GTCCTAG3200.09.8087441
CCGTACA4450.09.80213317
GTCCCGC4000.09.71968812
CCCGTAC4450.09.58904316
AACGGTT900.00109310519.5199586
CGCCCGC1106.693156E-59.51907410
CAGTGCG800.00444383459.51907359
CCCCGTA4300.09.48224815
Produced by FastQC (version 0.11.5)