FastQCFastQC Report
Wed 25 May 2016
SRR1294613_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294613_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670553
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC114261.7039667259709523No Hit
CCCATGTACTCTGCGTTGATACCAC72621.0829867288640869No Hit
GTATCAACGCAGAGTACTTTTTTTT51430.7669788965227209No Hit
GAGTACATGGGAAGCAGTGGTATCA50460.7525132241597607No Hit
GGTATCAACGCAGAGTACTTTTTTT38320.5714686236583835No Hit
TATCAACGCAGAGTACTTTTTTTTT30150.44962888839510073No Hit
GTATCAACGCAGAGTACATGGGAAG28350.4227853726700201No Hit
CATGTACTCTGCGTTGATACCACTG26230.3911696763715918No Hit
GTATTTAGCCTTGGACGGAGTCTAC24900.37133530086361555No Hit
ACGCAGAGTACATGGGAAGCAGTGG24440.3644752912894283No Hit
GCGTTGATACCACTGCTTCCCATGT23910.3565713672148212No Hit
GTACTTGTTCGCTATCGGTCTCTCG23650.3526939704989762No Hit
GCTTCCCATGTACTCTGCGTTGATA21870.3261487160597298No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA21680.3233152338443046No Hit
TATCAACGCAGAGTACATGGGAAGC20820.3104899985534328No Hit
GGTATCAACGCAGAGTACATGGGAA20270.3022878131929915No Hit
GTGGTATCAACGCAGAGTACATGGG19800.29527867297588706No Hit
TTCCCAAACAACCCGACTCGTTGAC18130.27037385560872895No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16170.24114424959697442No Hit
ACTCTGCGTTGATACCACTGCTTCC15310.22831901430610257No Hit
GCCTTGGACGGAGTCTACCGCCCGA14670.21877465315940725No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12860.19178200679140947No Hit
CAGTGGTATCAACGCAGAGTACATG12280.1831324295022168No Hit
CTATCGGTCTCTCGCCTGTATTTAG11940.17806198764303494No Hit
GGGAAGCAGTGGTATCAACGCAGAG11400.17000893292551075No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11290.16836849585342248No Hit
CATGGGAAGCAGTGGTATCAACGCA11220.16732458135300265No Hit
GCAGTGGTATCAACGCAGAGTACAT10020.1494289042029489No Hit
GAGTCTACCGCCCGATTTGGGCTGC9740.1452532462012697No Hit
ACATGGGAAGCAGTGGTATCAACGC9730.1451041155583526No Hit
CGCCTGTATTTAGCCTTGGACGGAG8760.13063844319539245No Hit
CTGCTTCCCATGTACTCTGCGTTGA8250.12303278040661961No Hit
CCTGTATTTAGCCTTGGACGGAGTC8080.12049755947702867No Hit
ATGGGAAGCAGTGGTATCAACGCAG7950.11855886111910617No Hit
CGGTACTTGTTCGCTATCGGTCTCT7920.11811146919035481No Hit
GCAGAGTACATGGGAAGCAGTGGTA7770.11587450954659811No Hit
AAGCAGTGGTATCAACGCAGAGTAC7550.1125936354024216No Hit
GTACATGGGGCGACCCCAGGTCAGG7520.11214624347367023No Hit
ATACCACTGCTTCCCATGTACTCTG7400.11035667575866487No Hit
GTACTCTGCGTTGATACCACTGCTT7350.1096110225440793No Hit
GTCTACCGCCCGATTTGGGCTGCAT6930.10334753554156048No Hit
GTCTCTCGCCTGTATTTAGCCTTGG6760.10081231461196952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAT250.006031309718.9985081
GTCTTAA453.5364217E-516.8875621
CAGGCGA653.3497345E-614.62187616
TCAATCT400.00525943614.25632913
AGAACTG954.9021764E-913.9957665
CTTTCGC500.001492989613.30590617
TACACCG500.001501412813.29597957
AACTGGT802.0013504E-613.0585517
ACCGTGC802.0013504E-613.0585518
AGGCGAG751.4688894E-512.67229217
ACAGGCG751.4688894E-512.67229215
ACGGACA751.4688894E-512.67229214
GTACCGT604.0970708E-412.6628366
TACCGTG701.092195E-412.2105917
TTCGCCG550.003068833112.08905719
TACAGGG550.00307226612.0872532
CACCCAC550.00307226612.0872537
AAATCGG951.0294261E-612.00712218
CAGAACT951.0406275E-611.996374
TACGGAC802.8476872E-511.88027413
Produced by FastQC (version 0.11.5)