FastQCFastQC Report
Wed 25 May 2016
SRR1294613_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294613_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670553
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT129831.9361631369928998No Hit
GTACATGGGAAGCAGTGGTATCAAC118481.7668998572819747No Hit
GGTATCAACGCAGAGTACTTTTTTT108391.6164270385786061No Hit
TATCAACGCAGAGTACTTTTTTTTT85251.2713387308684025No Hit
CCCATGTACTCTGCGTTGATACCAC79071.1791759935456259No Hit
GAGTACATGGGAAGCAGTGGTATCA57650.8597381564171662No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53230.7938224122478015No Hit
ACGCAGAGTACATGGGAAGCAGTGG31580.4709545703322482No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30710.4579802043984592No Hit
CATGTACTCTGCGTTGATACCACTG29850.44515496910758734No Hit
GTATCAACGCAGAGTACATGGGAAG28580.42621537745711374No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27140.40474056487704924No Hit
GCGTTGATACCACTGCTTCCCATGT25200.375809220151129No Hit
GCTTCCCATGTACTCTGCGTTGATA24500.365370075146931No Hit
TATCAACGCAGAGTACATGGGAAGC23800.3549309301427329No Hit
GGTATCAACGCAGAGTACATGGGAA23450.34971135764063394No Hit
GTGGTATCAACGCAGAGTACATGGG21500.32063088227179654No Hit
GTACTTGTTCGCTATCGGTCTCTCG20330.3031825970504941No Hit
GTATTTAGCCTTGGACGGAGTCTAC20010.29841041647714645No Hit
TTCCCAAACAACCCGACTCGTTGAC17310.2581451428895255No Hit
GTGGTATCAACGCAGAGTACTTTTT17280.2576977509607742No Hit
ACTCTGCGTTGATACCACTGCTTCC16910.25217991717284094No Hit
CATGGGAAGCAGTGGTATCAACGCA16170.24114424959697442No Hit
CAGTGGTATCAACGCAGAGTACATG15370.22921379816360524No Hit
GCCTTGGACGGAGTCTACCGCCCGA14440.2153446483723136No Hit
GCAGAGTACTTTTTTTTTTTTTTTT14060.20967768394146322No Hit
GGGAAGCAGTGGTATCAACGCAGAG14030.2092302920127119No Hit
ATCAACGCAGAGTACTTTTTTTTTT13520.20162462922393903No Hit
CTATCGGTCTCTCGCCTGTATTTAG13090.19521201157850313No Hit
ACATGGGAAGCAGTGGTATCAACGC12640.18850113264723298No Hit
GCAGTGGTATCAACGCAGAGTACAT12320.1837289520738853No Hit
ATGGGAAGCAGTGGTATCAACGCAG10330.15405195413337947No Hit
CGCCTGTATTTAGCCTTGGACGGAG9730.1451041155583526No Hit
GCAGAGTACATGGGAAGCAGTGGTA9610.1433145478433472No Hit
CTGCTTCCCATGTACTCTGCGTTGA9510.14182324141417604No Hit
CCTGTATTTAGCCTTGGACGGAGTC8940.13332279476790054No Hit
GAGTCTACCGCCCGATTTGGGCTGC8920.1330245334820663No Hit
AAGCAGTGGTATCAACGCAGAGTAC8680.12944539805205554No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA8310.1239275642641223No Hit
ATACCACTGCTTCCCATGTACTCTG8250.12303278040661961No Hit
GGAAGCAGTGGTATCAACGCAGAGT7990.11915538369077464No Hit
CGGTACTTGTTCGCTATCGGTCTCT7550.1125936354024216No Hit
GTACTCTGCGTTGATACCACTGCTT7520.11214624347367023No Hit
GTACATGGGGCGACCCCAGGTCAGG7240.10797058547199104No Hit
GATACCACTGCTTCCCATGTACTCT7180.10707580161448833No Hit
GTCCGGGCCGGACGGGGCTCTCACC7130.10633014839990278No Hit
GGTATCAACGCAGAGTACATGGGGA6890.10275101296989202No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCA250.005953255619.0413937
TTAGGGT453.4686702E-516.9256824
CCAGTAC402.769027E-416.6164443
AAGCCCC350.002192264716.25912717
TAGGGTG400.005199324314.2810445
CCGTGCA801.2532291E-714.2789079
TTAATAC602.5764355E-514.2426653
TTCGGTA602.5764355E-514.2426652
CGGTACC551.9217162E-413.8482854
AGTACTC1400.013.6009955
TAAAACC500.001473621213.3289764
ACCGTGC801.946395E-613.0909588
TACCGTG751.442949E-512.6942627
GGGAAAC604.012264E-412.6942617
CTTCGGT701.08448425E-412.2198581
GTACTAG701.08448425E-412.2198581
AAAGTAC1251.4151738E-912.1537423
AATACCG550.003015742212.11724955
CCTTTGG550.003054854712.0964251
GTACCGT657.867957E-411.717786
Produced by FastQC (version 0.11.5)