FastQCFastQC Report
Wed 25 May 2016
SRR1294612_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294612_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2633756
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC221860.8423711232171848No Hit
CCCATGTACTCTGCGTTGATACCAC218030.8278291534978943No Hit
GTATCAACGCAGAGTACTTTTTTTT119810.45490166894731326No Hit
GAGTACATGGGAAGCAGTGGTATCA98590.374332322356361No Hit
GGTATCAACGCAGAGTACTTTTTTT83080.31544304028163583No Hit
CATGTACTCTGCGTTGATACCACTG79370.3013566936344901No Hit
TATCAACGCAGAGTACTTTTTTTTT78320.29736999175322243No Hit
GCGTTGATACCACTGCTTCCCATGT64290.2441000609016173No Hit
GTATCAACGCAGAGTACATGGGAAG55540.21087754522438676No Hit
ACGCAGAGTACATGGGAAGCAGTGG53170.2018789895495255No Hit
TATCAACGCAGAGTACATGGGAAGC51990.19739869600676752No Hit
GGTATCAACGCAGAGTACATGGGAA45060.17108646359040092No Hit
ACTCTGCGTTGATACCACTGCTTCC44970.1707447462862923No Hit
GTACTGGTTCACTATCGGTCAGTCA42730.16223978227292124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA38250.1452298542461792No Hit
GTTAATGATAGTGTGTCGAAACACA34920.1325863139941589No Hit
ACGCAGAGTACTTTTTTTTTTTTTT34760.1319788165646324No Hit
GCTTCCCATGTACTCTGCGTTGATA30900.11732294107730555No Hit
GTGGTATCAACGCAGAGTACATGGG30430.11553841737807147No Hit
GGTATCAACGCAGAGTACATGGGGG26610.10103441624812624No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTA456.777213E-414.77412819
GCGTAGA604.0976418E-412.6652221
AATCGCC1950.012.18643618
AAATCGC1950.012.18597217
ATCGCCG1900.011.99703519
GAAATCG2200.011.66507616
TCGAATT600.005856740311.08860213
GGTCGGT600.005891642511.07975411
ACCGTCC700.001496023710.8536378
TAGGACA2250.010.1300614
CGCAGAA2550.010.0555742
ATTAGAC1803.965397E-1010.024543
ACGATTC951.6501769E-49.9967698
CTAGACT2002.5465852E-119.9717784
GTGTAGA3250.09.9373271
TACTGGT9950.09.9264412
GTACTGG11200.09.9229761
TAGGACC1554.0869054E-89.8032854
GTACTAG2054.1836756E-119.7305981
GACAGAT2251.8189894E-129.7079757
Produced by FastQC (version 0.11.5)