##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294612_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2633756 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.740507472977754 31.0 30.0 33.0 25.0 34.0 2 29.89289516568733 31.0 30.0 34.0 25.0 34.0 3 29.85190085945699 31.0 30.0 34.0 25.0 34.0 4 33.36222451890001 35.0 33.0 37.0 26.0 37.0 5 33.862838850675615 35.0 35.0 37.0 28.0 37.0 6 33.86179737226987 37.0 35.0 37.0 28.0 37.0 7 33.81843458543616 37.0 35.0 37.0 28.0 37.0 8 33.66107300752234 37.0 35.0 37.0 27.0 37.0 9 34.67687933126683 39.0 35.0 39.0 25.0 39.0 10 34.87929026075309 39.0 35.0 39.0 27.0 39.0 11 35.08096649803551 39.0 35.0 39.0 27.0 39.0 12 34.90304492899114 38.0 35.0 39.0 27.0 39.0 13 34.695182089760785 38.0 35.0 39.0 26.0 39.0 14 35.5831474138075 39.0 34.0 41.0 25.0 41.0 15 35.554130299086175 39.0 34.0 41.0 25.0 41.0 16 35.667199619099115 39.0 34.0 41.0 26.0 41.0 17 35.7105692402789 39.0 34.0 41.0 26.0 41.0 18 35.596006615647006 39.0 34.0 41.0 25.0 41.0 19 35.87570792434835 39.0 34.0 41.0 27.0 41.0 20 35.99679089482853 39.0 35.0 41.0 27.0 41.0 21 36.04818821485362 39.0 35.0 41.0 27.0 41.0 22 36.08952993367647 39.0 35.0 41.0 27.0 41.0 23 36.08884422095289 39.0 35.0 41.0 27.0 41.0 24 36.114654128932216 39.0 35.0 41.0 27.0 41.0 25 34.95597124410917 38.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.993419171944101 1101 2 1.0004396974191714 1101 3 1.284728701429426 1101 4 1.090512134222955 1101 5 0.8305782100906072 1101 6 0.6821915895169255 1101 7 0.6918299418620677 1101 8 0.8452238833226389 1101 9 1.1368986336538 1101 10 0.9816427711553288 1101 11 0.8751324130421878 1101 12 1.0934147035779418 1101 13 1.15226256326509 1101 14 1.2323351154012059 1101 15 1.4943173283839428 1101 16 1.3833623100433456 1101 17 1.2080908370279602 1101 18 1.2556411722502432 1101 19 1.207141361607917 1101 20 0.990279870314744 1101 21 0.9146573428854197 1101 22 0.9396348967673092 1101 23 0.9220206996800968 1101 24 0.9458755127353058 1101 25 1.1341297757768345 1102 1 0.8714587692517597 1102 2 0.836744566567333 1102 3 1.175239481211399 1102 4 1.0189186843451736 1102 5 0.7606153398071243 1102 6 0.7244041962431353 1102 7 0.6412906631401185 1102 8 0.7979530043736247 1102 9 1.1194111743458848 1102 10 1.0491525314630223 1102 11 0.7697283305111071 1102 12 0.9491257868852117 1102 13 1.026405278078748 1102 14 1.2567098185372458 1102 15 1.259105497848374 1102 16 1.1461694556312239 1102 17 1.1862952300352916 1102 18 1.2635276167178517 1102 19 1.0582199190518153 1102 20 0.27033553592718107 1102 21 0.5376560453726995 1102 22 0.7143499073675272 1102 23 0.7634156996221719 1102 24 0.9707787785284694 1102 25 0.9153975152084755 1103 1 0.6724678316106996 1103 2 0.8011015864679614 1103 3 0.9221574934415351 1103 4 0.8069040232555906 1103 5 0.5772370675001923 1103 6 0.405683966809832 1103 7 0.4573304692090048 1103 8 0.45729766505430547 1103 9 0.7322868918260639 1103 10 0.7629362086336258 1103 11 0.6650381608815152 1103 12 0.9223828883254228 1103 13 0.7938571941787629 1103 14 0.5436106489260908 1103 15 0.9348388930780231 1103 16 0.8892883005753305 1103 17 0.9114670284040187 1103 18 0.9705965052379284 1103 19 0.8416367963824456 1103 20 0.06503622472418868 1103 21 0.3915226812515584 1103 22 -0.1536758325220049 1103 23 0.3760148479865322 1103 24 0.5262860276940344 1103 25 0.7011914277213904 1104 1 0.5209869935622642 1104 2 0.4449159242180549 1104 3 0.7300678673063494 1104 4 0.5537621014774956 1104 5 0.4243197829511729 1104 6 0.3194143125519986 1104 7 0.33825510115907775 1104 8 0.42196671206775704 1104 9 0.7627606096301918 1104 10 0.7353824948607297 1104 11 0.3922265042209432 1104 12 0.6083610780780901 1104 13 0.6321828569725696 1104 14 0.8601849331823743 1104 15 0.8754975516694259 1104 16 0.7147251374016008 1104 17 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0.21386085213547545 2207 8 0.3337224094053681 2207 9 -1.2021820269210437 2207 10 -0.1311803244448626 2207 11 0.21206924270712335 2207 12 0.5122690294285945 2207 13 0.5885338774451654 2207 14 0.8231638498306708 2207 15 0.8449073550303083 2207 16 0.7965124473208576 2207 17 0.7650387065140976 2207 18 0.825369197887639 2207 19 -0.3540502648011028 2207 20 0.20175887841040918 2207 21 -0.2930423194223266 2207 22 0.2421490612686128 2207 23 -0.5885035263217802 2207 24 -1.6279721407969774 2207 25 -0.28024954536917335 2208 1 0.5839072949961803 2208 2 0.26199164673294817 2208 3 0.7224727516577154 2208 4 0.6053254803302792 2208 5 0.46634104923023045 2208 6 0.4311024111043622 2208 7 0.4918364192386946 2208 8 0.6006500796413832 2208 9 -0.06535805423757068 2208 10 0.2536607677177969 2208 11 0.3853774763020539 2208 12 0.4378841160497515 2208 13 0.7141803713810901 2208 14 0.9482226139136642 2208 15 0.9345418372707499 2208 16 0.7216056455006594 2208 17 0.7038210783930836 2208 18 0.9272389301741057 2208 19 -1.2362766445953 2208 20 0.04652794028246632 2208 21 -0.05596402582945359 2208 22 0.34554418487348926 2208 23 0.5964017336200484 2208 24 0.7274481670588031 2208 25 0.7867845963760942 2209 1 0.0010056564796094847 2209 2 -1.5961161913031319 2209 3 -0.3185391091097465 2209 4 -0.07033105529340133 2209 5 -0.06767405266690218 2209 6 0.04845290330447227 2209 7 -0.04200452979099367 2209 8 0.16716707839177047 2209 9 -2.1326263260885483 2209 10 -0.5585593500063126 2209 11 -0.1694008758689165 2209 12 0.25747850501056035 2209 13 0.29359467498700553 2209 14 0.5147425228086107 2209 15 0.5010639550705989 2209 16 0.5055303926687031 2209 17 0.19186978088936257 2209 18 0.3791558038233447 2209 19 0.16581480266516735 2209 20 0.08610001579756243 2209 21 0.24625543272938444 2209 22 0.1573385449432223 2209 23 0.22102397581980426 2209 24 0.29648493024877354 2209 25 0.37645999955383047 2210 1 0.13282263904977754 2210 2 0.16382457853541155 2210 3 0.07674509236493421 2210 4 0.25869982033024286 2210 5 0.0590911959936804 2210 6 0.12531810199004667 2210 7 0.11330098784124942 2210 8 0.19685600487824928 2210 9 0.33109523491865644 2210 10 -1.5369834759321748 2210 11 -0.5373944628742962 2210 12 -2.306537392068833 2210 13 -0.7496281968173122 2210 14 -0.5541931908987792 2210 15 -0.6883642358618687 2210 16 -0.22595407459487404 2210 17 0.02542883934895457 2210 18 0.18100034244788787 2210 19 0.17053074078548747 2210 20 0.23918436747646865 2210 21 0.13677183653864944 2210 22 0.12480411383475598 2210 23 0.05308273694203791 2210 24 0.061060742901105414 2210 25 0.0538092439260609 2211 1 -0.289357394652658 2211 2 -0.14449725213670916 2211 3 -1.4409196569496245 2211 4 -0.5199364357676188 2211 5 -0.43836312356403084 2211 6 -0.3900889325585766 2211 7 -0.521144003312223 2211 8 -0.12932107659542424 2211 9 0.05538834740089271 2211 10 -0.01650452979365724 2211 11 -0.2959103510611669 2211 12 -0.23266331028600007 2211 13 -0.3134511298580378 2211 14 -0.7993438576176999 2211 15 -0.35350028658098154 2211 16 -0.17677094497113188 2211 17 -0.13963311536321754 2211 18 -0.045129276941025864 2211 19 -0.07365275895542567 2211 20 0.03477931994906669 2211 21 -0.20277779617448033 2211 22 -0.23602478897828405 2211 23 -0.6162584254581205 2211 24 -0.4844255692949062 2211 25 -0.3036243055266752 2212 1 -0.4305053737195763 2212 2 -0.44861999892313165 2212 3 -2.4965744961781198 2212 4 -1.600858351169272 2212 5 -0.6936655212183425 2212 6 -0.6131529009362637 2212 7 -0.40059011794929944 2212 8 -0.4891187667808836 2212 9 -1.0843344006227085 2212 10 -0.38628626036066294 2212 11 -0.44349537623777735 2212 12 -0.22603836750556638 2212 13 -0.521265157001757 2212 14 -0.21350972011754266 2212 15 -0.06897632240971063 2212 16 -1.0277142945115187 2212 17 -1.364752503206688 2212 18 -0.3606430467513704 2212 19 -0.1873835981344385 2212 20 -0.2014270540008738 2212 21 -0.03931666217554408 2212 22 -0.3005738678979739 2212 23 0.07235426305714299 2212 24 -0.13264990709446067 2212 25 0.08867889010855379 2213 1 -3.2039078162075043 2213 2 -1.4587280017001234 2213 3 -2.4982777686070357 2213 4 -1.1938026693869475 2213 5 -0.8838896526204039 2213 6 -0.7674308049408083 2213 7 -0.5360235491083785 2213 8 -0.29304481367412194 2213 9 -0.42778796495102256 2213 10 -0.2515537822136693 2213 11 -0.6449217386979385 2213 12 -0.4168975773260044 2213 13 -0.6714229404597418 2213 14 -0.32150736914402245 2213 15 -0.4790425125703308 2213 16 -1.5623335630274084 2213 17 -1.1442950889619965 2213 18 -0.46202641321922755 2213 19 -0.2787023522762553 2213 20 -0.28279992786877983 2213 21 -0.06838826430877987 2213 22 -0.12966164283565007 2213 23 -0.0921768045057405 2213 24 -0.09435980642861352 2213 25 -0.1373151456767303 2214 1 -1.0195475815117838 2214 2 -0.9203010956042306 2214 3 -6.323026516380139 2214 4 -2.4048987552096754 2214 5 -1.4679891142919317 2214 6 -1.0228119918453515 2214 7 -0.8311456735048566 2214 8 -0.5489762703883869 2214 9 -0.4437499850382949 2214 10 -0.4391091319335345 2214 11 -1.060125967868366 2214 12 -0.7146837499009493 2214 13 -5.323231246467849 2214 14 -3.836065636423463 2214 15 -2.5129878664212555 2214 16 -2.796040412837634 2214 17 -3.343143556597738 2214 18 -1.3322610121193321 2214 19 -0.9400892258400617 2214 20 -0.5329656542894767 2214 21 -0.6969014385103236 2214 22 -0.5008354119095202 2214 23 -0.5660836878808126 2214 24 -0.5118075550021075 2214 25 -0.3275548761252125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1009.0 3 132.0 4 140.0 5 297.0 6 37.0 7 39.0 8 63.0 9 200.0 10 614.0 11 1565.0 12 3588.0 13 7176.0 14 11598.0 15 17437.0 16 22247.0 17 25057.0 18 25580.0 19 24802.0 20 23985.0 21 23133.0 22 22486.0 23 22218.0 24 22464.0 25 23923.0 26 25546.0 27 28558.0 28 33241.0 29 40327.0 30 50281.0 31 63292.0 32 83187.0 33 111566.0 34 149563.0 35 205789.0 36 288748.0 37 438212.0 38 835656.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.69594689355336 20.664529912093567 12.75492732487981 22.884595869473255 2 14.919901077736034 22.66535479434882 38.89969875055559 23.515045377359566 3 18.701154438212406 28.581425352052747 28.983168938211644 23.7342512715232 4 12.03067756870885 19.001508882348205 36.528210629643084 32.439602919299865 5 12.180103248255454 40.36068513080772 34.15914210500325 13.300069515933583 6 29.644747235612133 38.53425416904607 18.006297215342045 13.81470137999975 7 25.164537609709154 35.16570399073278 22.438891322543107 17.23086707701496 8 24.464616516192958 37.57638294735978 20.924748454864726 17.034252081582537 9 25.338952881132737 18.189492945106824 20.594695370787953 35.87685880297249 10 15.415150412190723 31.5729204106026 32.162122525772844 20.849806651433834 11 31.092358437449423 27.797381464182617 21.98842166769624 19.121838430671716 12 20.88720609332069 29.76139458263428 30.25213819388723 19.099261130157803 13 27.22779649941302 24.897402483919866 25.509606288443198 22.365194728223912 14 20.35031849553696 25.81945258128418 27.269266453449802 26.560962469729056 15 24.51135012691884 30.563180733108737 23.482929501819342 21.442539638153082 16 20.61502304788253 31.34648795708721 26.670691292106124 21.367797702924136 17 20.868463075444836 30.7826015594813 27.5106391304959 20.838296234577964 18 21.55185000981014 28.915044191256655 28.692186718135677 20.840919080797526 19 21.361773752011352 30.17623915484235 29.024007051845885 19.437980041300413 20 22.74829316849476 28.02942170990893 28.368660407967873 20.853624713628438 21 22.03019056306506 28.90332492828098 28.56933902247861 20.497145486175356 22 22.106892515153092 28.466954993045135 27.144258048435944 22.281894443365825 23 21.270685695961873 28.93303677946757 28.267234343916364 21.52904318065419 24 21.628558672378908 29.610851991526633 27.402000550958956 21.358588785135506 25 21.870674545745377 27.939903090827368 27.437043802900433 22.752378560526818 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5995.0 1 5995.0 2 6140.5 3 6286.0 4 6286.0 5 6286.0 6 8690.5 7 11095.0 8 11095.0 9 11095.0 10 14053.5 11 17012.0 12 17012.0 13 17012.0 14 23578.5 15 30145.0 16 30145.0 17 30145.0 18 44382.5 19 58620.0 20 58620.0 21 58620.0 22 79670.0 23 100720.0 24 100720.0 25 100720.0 26 129658.0 27 158596.0 28 158596.0 29 158596.0 30 184720.5 31 210845.0 32 210845.0 33 210845.0 34 230781.5 35 250718.0 36 250718.0 37 250718.0 38 255090.0 39 259462.0 40 259462.0 41 259462.0 42 268310.5 43 277159.0 44 277159.0 45 277159.0 46 320355.5 47 363552.0 48 363552.0 49 363552.0 50 332328.5 51 301105.0 52 301105.0 53 301105.0 54 255738.0 55 210371.0 56 210371.0 57 210371.0 58 179571.5 59 148772.0 60 148772.0 61 148772.0 62 128658.0 63 108544.0 64 108544.0 65 108544.0 66 84794.5 67 61045.0 68 61045.0 69 61045.0 70 46439.5 71 31834.0 72 31834.0 73 31834.0 74 22959.0 75 14084.0 76 14084.0 77 14084.0 78 9710.5 79 5337.0 80 5337.0 81 5337.0 82 3496.5 83 1656.0 84 1656.0 85 1656.0 86 1122.0 87 588.0 88 588.0 89 588.0 90 377.0 91 166.0 92 166.0 93 166.0 94 95.5 95 25.0 96 25.0 97 25.0 98 24.5 99 24.0 100 24.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10111035342681707 2 0.05262446483273318 3 0.05619351223120138 4 0.05102978408022611 5 0.048068234111284414 6 0.068305492232386 7 0.06390113586831886 8 0.06166098909693988 9 0.0665589371224973 10 0.0653819108527897 11 0.06067380577395932 12 0.06181286345432151 13 0.061736926275630696 14 0.06431879035111832 15 0.06678674865856973 16 0.06177489486497611 17 0.06557175379951674 18 0.1451918856568338 19 0.06914080119798494 20 0.06401504163635507 21 0.06109146025675879 22 0.06777393198155031 23 0.06621721981838864 24 0.07521577549324995 25 0.06860924094714924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2633756.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.00759624529055 #Duplication Level Percentage of deduplicated Percentage of total 1 81.8134410452953 49.912413787292586 2 11.672253526155412 14.241922607927169 3 3.227091894188637 5.906313579813308 4 1.2596342075344273 3.073890206000675 5 0.5937486770655676 1.811158978079578 6 0.35151343072471397 1.2866993673870155 7 0.20887205861047434 0.891994755304134 8 0.1439508978942867 0.7025678606305352 9 0.104683999295671 0.574786724613532 >10 0.5478535644446517 6.016420177133922 >50 0.04009075228267319 1.7120884892001371 >100 0.028997342259973018 3.584960063844189 >500 0.0032733393672328167 1.3991572832545847 >1k 0.0039028277070754557 4.568872779855784 >5k 5.03590671880704E-4 2.1673054999677333 >10k+ 1.8884650195526398E-4 2.1494478396951187 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 22186 0.8423711232171848 No Hit CCCATGTACTCTGCGTTGATACCAC 21803 0.8278291534978943 No Hit GTATCAACGCAGAGTACTTTTTTTT 11981 0.45490166894731326 No Hit GAGTACATGGGAAGCAGTGGTATCA 9859 0.374332322356361 No Hit GGTATCAACGCAGAGTACTTTTTTT 8308 0.31544304028163583 No Hit CATGTACTCTGCGTTGATACCACTG 7937 0.3013566936344901 No Hit TATCAACGCAGAGTACTTTTTTTTT 7832 0.29736999175322243 No Hit GCGTTGATACCACTGCTTCCCATGT 6429 0.2441000609016173 No Hit GTATCAACGCAGAGTACATGGGAAG 5554 0.21087754522438676 No Hit ACGCAGAGTACATGGGAAGCAGTGG 5317 0.2018789895495255 No Hit TATCAACGCAGAGTACATGGGAAGC 5199 0.19739869600676752 No Hit GGTATCAACGCAGAGTACATGGGAA 4506 0.17108646359040092 No Hit ACTCTGCGTTGATACCACTGCTTCC 4497 0.1707447462862923 No Hit GTACTGGTTCACTATCGGTCAGTCA 4273 0.16223978227292124 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 3825 0.1452298542461792 No Hit GTTAATGATAGTGTGTCGAAACACA 3492 0.1325863139941589 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3476 0.1319788165646324 No Hit GCTTCCCATGTACTCTGCGTTGATA 3090 0.11732294107730555 No Hit GTGGTATCAACGCAGAGTACATGGG 3043 0.11553841737807147 No Hit GGTATCAACGCAGAGTACATGGGGG 2661 0.10103441624812624 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.796858934540633E-5 2 0.0 0.0 0.0 0.0 3.796858934540633E-5 3 0.0 0.0 0.0 0.0 3.796858934540633E-5 4 0.0 0.0 0.0 0.0 3.796858934540633E-5 5 0.0 0.0 0.0 3.796858934540633E-5 3.796858934540633E-5 6 0.0 0.0 0.0 3.796858934540633E-5 3.796858934540633E-5 7 0.0 0.0 0.0 3.796858934540633E-5 3.796858934540633E-5 8 3.796858934540633E-5 0.0 0.0 7.593717869081266E-5 3.796858934540633E-5 9 3.796858934540633E-5 0.0 0.0 7.593717869081266E-5 3.796858934540633E-5 10 3.796858934540633E-5 0.0 0.0 1.13905768036219E-4 3.796858934540633E-5 11 3.796858934540633E-5 0.0 0.0 1.13905768036219E-4 3.796858934540633E-5 12 3.796858934540633E-5 0.0 0.0 1.13905768036219E-4 3.796858934540633E-5 13 3.796858934540633E-5 0.0 0.0 1.13905768036219E-4 3.796858934540633E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGTTA 45 6.777213E-4 14.774128 19 GCGTAGA 60 4.0976418E-4 12.665222 1 AATCGCC 195 0.0 12.186436 18 AAATCGC 195 0.0 12.185972 17 ATCGCCG 190 0.0 11.997035 19 GAAATCG 220 0.0 11.665076 16 TCGAATT 60 0.0058567403 11.088602 13 GGTCGGT 60 0.0058916425 11.079754 11 ACCGTCC 70 0.0014960237 10.853637 8 TAGGACA 225 0.0 10.130061 4 CGCAGAA 255 0.0 10.055574 2 ATTAGAC 180 3.965397E-10 10.02454 3 ACGATTC 95 1.6501769E-4 9.996769 8 CTAGACT 200 2.5465852E-11 9.971778 4 GTGTAGA 325 0.0 9.937327 1 TACTGGT 995 0.0 9.926441 2 GTACTGG 1120 0.0 9.922976 1 TAGGACC 155 4.0869054E-8 9.803285 4 GTACTAG 205 4.1836756E-11 9.730598 1 GACAGAT 225 1.8189894E-12 9.707975 7 >>END_MODULE