FastQCFastQC Report
Wed 25 May 2016
SRR1294612_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294612_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2633756
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT303381.1518910635609372No Hit
CCCATGTACTCTGCGTTGATACCAC242860.9221051608425382No Hit
GGTATCAACGCAGAGTACTTTTTTT226060.8583179307422556No Hit
GTACATGGGAAGCAGTGGTATCAAC218470.8294997714290921No Hit
TATCAACGCAGAGTACTTTTTTTTT217840.8271077503003316No Hit
ACGCAGAGTACTTTTTTTTTTTTTT122950.46682380600177087No Hit
GAGTACATGGGAAGCAGTGGTATCA103600.3933545856184096No Hit
CATGTACTCTGCGTTGATACCACTG92980.3530319437335881No Hit
GTACTTTTTTTTTTTTTTTTTTTTT84310.3201131767711208No Hit
GAGTACTTTTTTTTTTTTTTTTTTT67370.25579438642000246No Hit
GCGTTGATACCACTGCTTCCCATGT67110.2548072030970219No Hit
ACGCAGAGTACATGGGAAGCAGTGG64520.24497333845656166No Hit
TATCAACGCAGAGTACATGGGAAGC57450.2181295457893594No Hit
GTATCAACGCAGAGTACATGGGAAG55980.21254816315558464No Hit
ACTCTGCGTTGATACCACTGCTTCC51420.19523448641407937No Hit
GGTATCAACGCAGAGTACATGGGAA49510.18798248584910676No Hit
GCAGAGTACTTTTTTTTTTTTTTTT42590.16170822202208557No Hit
GTACTGGTTCACTATCGGTCAGTCA38520.1462550061585052No Hit
GCTTCCCATGTACTCTGCGTTGATA34760.1319788165646324No Hit
GTTAATGATAGTGTGTCGAAACACA33890.12867554929158206No Hit
GTGGTATCAACGCAGAGTACATGGG32680.1240813499807879No Hit
CAGTGGTATCAACGCAGAGTACATG30170.1145512340550909No Hit
ATCAACGCAGAGTACTTTTTTTTTT29050.1102987520484054No Hit
GGTATCAACGCAGAGTACATGGGGG28710.10900782001066157No Hit
GGATACCACGTGTCCCGCCCTACTC26910.10217347392848844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTCG500.001520651513.2759214
CACGTCA1001.8857681E-611.4225139
CTCGTCG600.00595393411.06410817
TACTCCG1303.169407E-810.9846515
CAGTACT2100.010.8800354
GACTCGT750.00268872710.11498615
TCACGTC856.4696645E-410.0800338
ACTCACG951.6102119E-410.0210866
CGCCCTA6200.09.942416
TGTCCCG6300.09.940411
CCAGTAC2251.8189894E-129.7104883
TTAGACT1401.0292206E-69.5200314
GTATTAA3700.09.5095191
GTACTGG12500.09.5095191
CCCCCGG7050.09.14722518
GCACCGA1054.400442E-49.0666966
GTATAGG2850.09.0090181
TACTGGT11950.08.9826882
TAATCAG1701.6323429E-78.960035
ACGGTGA850.00731020658.960038
Produced by FastQC (version 0.11.5)