##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294612_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2633756 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.132597704570962 31.0 31.0 34.0 28.0 34.0 2 31.319484796617456 31.0 31.0 34.0 28.0 34.0 3 31.45285554166749 31.0 31.0 34.0 28.0 34.0 4 35.027875019553825 37.0 35.0 37.0 32.0 37.0 5 35.21529557028062 37.0 35.0 37.0 32.0 37.0 6 34.68952401057653 37.0 35.0 37.0 32.0 37.0 7 34.54882798558408 37.0 35.0 37.0 31.0 37.0 8 34.57223903808857 37.0 35.0 37.0 31.0 37.0 9 35.99640513396078 39.0 35.0 39.0 30.0 39.0 10 35.830658193090024 39.0 35.0 39.0 30.0 39.0 11 35.956376368957486 39.0 35.0 39.0 30.0 39.0 12 35.9301700689054 39.0 35.0 39.0 30.0 39.0 13 35.76112593573588 39.0 35.0 39.0 30.0 39.0 14 36.796504307916145 40.0 36.0 41.0 30.0 41.0 15 36.825794796480764 40.0 36.0 41.0 30.0 41.0 16 36.85676615449571 39.0 36.0 41.0 30.0 41.0 17 36.95749112674067 40.0 36.0 41.0 30.0 41.0 18 36.97409327211784 40.0 36.0 41.0 30.0 41.0 19 36.97702938313192 40.0 36.0 41.0 30.0 41.0 20 37.06133028268374 40.0 36.0 41.0 30.0 41.0 21 36.903135294233785 40.0 36.0 41.0 30.0 41.0 22 36.93393161705185 40.0 36.0 41.0 30.0 41.0 23 36.935225586576735 40.0 36.0 41.0 30.0 41.0 24 36.84823157498265 40.0 36.0 41.0 29.0 41.0 25 35.7514614110039 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5512471205657654 1101 2 0.49498547780205016 1101 3 0.4308634085813132 1101 4 0.41615544661397763 1101 5 0.3866053873437565 1101 6 0.5090717372544731 1101 7 0.5590058347522913 1101 8 0.5638326913106937 1101 9 0.7569015825194398 1101 10 0.7546543695323322 1101 11 0.8437527896562003 1101 12 0.8860694840371863 1101 13 0.7385034075803105 1101 14 0.9660235522646019 1101 15 0.9977514528717535 1101 16 0.9541924605347845 1101 17 1.00712897055962 1101 18 1.090305295019931 1101 19 1.1344523088351863 1101 20 1.087079759237362 1101 21 1.2855011665677836 1101 22 1.2046096210321195 1101 23 1.160064204368183 1101 24 1.2485099702862712 1101 25 1.1784048845560946 1102 1 0.4049757318796132 1102 2 0.4215413345659158 1102 3 0.3655982294202964 1102 4 0.3318460825110563 1102 5 0.34265069602435005 1102 6 0.3650246560788375 1102 7 0.48499113346984046 1102 8 0.412818719879148 1102 9 0.5951773387260673 1102 10 0.6242557898453569 1102 11 0.6482729416505464 1102 12 0.7250316476396392 1102 13 0.7906167893386709 1102 14 0.9169671452163257 1102 15 0.9516694778720449 1102 16 0.833791679710167 1102 17 0.7928757354665379 1102 18 0.8705608007602663 1102 19 0.9608115113282665 1102 20 0.8620047467835548 1102 21 1.1741670454588728 1102 22 1.0896378303487282 1102 23 0.9630392156634926 1102 24 1.1234057058717113 1102 25 1.0905679077607147 1103 1 0.40374341783115497 1103 2 0.40241473013231044 1103 3 0.27741986539135866 1103 4 0.3075294074659354 1103 5 0.23537314795247966 1103 6 0.380041328798832 1103 7 0.3293987768636484 1103 8 0.398094050266792 1103 9 0.4067703110884011 1103 10 0.562339946391738 1103 11 0.6011634677452165 1103 12 0.5970793655335314 1103 13 0.6447955125586873 1103 14 0.8469656539319246 1103 15 0.8089901609444397 1103 16 0.7408621717618615 1103 17 0.7264642307685918 1103 18 0.7595618165975466 1103 19 0.8309625319360521 1103 20 0.716954488093819 1103 21 0.9255158159910977 1103 22 0.959148681046301 1103 23 0.9328964692788588 1103 24 1.0435875944631476 1103 25 0.8819259146350547 1104 1 0.17426890977748855 1104 2 0.23561160944649018 1104 3 0.16628686511461055 1104 4 0.17887898250685197 1104 5 0.18546257092634022 1104 6 0.15120050009262087 1104 7 0.3168820800427241 1104 8 0.26454403818909356 1104 9 0.047965347553457605 1104 10 0.4383958653447664 1104 11 0.5081118647704272 1104 12 0.4713078324334319 1104 13 -0.1490496593205819 1104 14 0.40735043666929016 1104 15 0.5078274223298749 1104 16 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0.30322076452784685 2207 3 0.23426256707845283 2207 4 0.18829286265831513 2207 5 0.16421666365374676 2207 6 0.3558333794845794 2207 7 0.28364052762029957 2207 8 0.33274558767307383 2207 9 0.42369153536267135 2207 10 0.5424396287658482 2207 11 0.49508821877791576 2207 12 0.4319269612326906 2207 13 0.5289792018068695 2207 14 0.6182209119022559 2207 15 0.5601592372636119 2207 16 0.6003094133724929 2207 17 0.6034194588433763 2207 18 0.5749412628922741 2207 19 0.60017691280229 2207 20 0.5775486420822347 2207 21 0.9187585819746147 2207 22 0.7566694886155645 2207 23 0.6426957949299634 2207 24 0.8917859008402189 2207 25 0.8157004777610553 2208 1 0.3245443613539578 2208 2 0.2492211925504364 2208 3 0.2447482302802264 2208 4 0.19588577685473751 2208 5 0.23555104366018043 2208 6 0.37991691439565756 2208 7 0.3886995979424128 2208 8 0.21248633845033282 2208 9 0.46614656895460627 2208 10 0.5531890137059321 2208 11 0.23587264673213326 2208 12 0.03949341779321003 2208 13 0.1928756744663005 2208 14 -0.24375871749855094 2208 15 0.21161168313496148 2208 16 0.43813884743903486 2208 17 0.3503758158512511 2208 18 0.4420787781463602 2208 19 0.6207232077896165 2208 20 0.5559613121206226 2208 21 0.8898629265463356 2208 22 0.8057674897643139 2208 23 0.8254408939466913 2208 24 0.8921478683624855 2208 25 0.8225003038457217 2209 1 -0.02516606173197289 2209 2 -0.012226194139756785 2209 3 0.03845544589599115 2209 4 -0.5389364518812201 2209 5 -0.08868474536766513 2209 6 0.14201221146181098 2209 7 0.15093903481892568 2209 8 0.15101826519042305 2209 9 0.20995283467114234 2209 10 0.27251349552489046 2209 11 0.34729962734846964 2209 12 0.26636544716673427 2209 13 0.33369192443299056 2209 14 0.3639495892461113 2209 15 -0.27183597563621476 2209 16 -0.19651690700366942 2209 17 0.13628661396014508 2209 18 -0.28457670842279015 2209 19 0.26968330444849187 2209 20 0.26975231184994186 2209 21 0.6015792386428558 2209 22 0.5840171394951099 2209 23 0.4924605062172631 2209 24 0.5907138683372466 2209 25 0.6019569973885623 2210 1 -0.11362302040347672 2210 2 -0.18013776907854506 2210 3 -0.09236210622180607 2210 4 -0.009984587031517833 2210 5 -0.0597509580713691 2210 6 -0.5771596969973629 2210 7 -0.28394142997660765 2210 8 -0.3388101446270113 2210 9 -0.08114819302729614 2210 10 0.15352243239723862 2210 11 0.06094280575926092 2210 12 0.06745268287848916 2210 13 0.12279730191185223 2210 14 0.11412896883005885 2210 15 0.07662135994180375 2210 16 0.13127101467794233 2210 17 -0.9368267595207129 2210 18 -1.5096728893825926 2210 19 -0.37117161725267067 2210 20 -0.180966462840928 2210 21 -0.35201491400149365 2210 22 -0.37541403877050783 2210 23 -0.19527949249846444 2210 24 0.21255539170963544 2210 25 0.15013276983753343 2211 1 -0.26112023001026685 2211 2 -0.2873409750189957 2211 3 -0.2653361501220566 2211 4 -0.22011904842394614 2211 5 -0.12848338657706648 2211 6 -0.24814388936540155 2211 7 -0.2247724664063 2211 8 -0.22422114160657003 2211 9 -0.2260808534730927 2211 10 -0.05428072622627411 2211 11 -0.35468462993832617 2211 12 -0.30612690174844204 2211 13 -0.14740224204572883 2211 14 -0.21940575178953026 2211 15 -0.23896289541872306 2211 16 -0.3203140302658909 2211 17 -0.4263180443498271 2211 18 -0.44891830029248325 2211 19 -0.26961023077921453 2211 20 -0.3212143832904104 2211 21 -0.035832375113784565 2211 22 -0.20595313257851444 2211 23 -0.8571141705908687 2211 24 -0.3028430067715746 2211 25 -0.9175721117902853 2212 1 -0.20249971633324293 2212 2 -0.3312107494811336 2212 3 -0.17809966940350108 2212 4 -0.08856044940626617 2212 5 -0.0861919441492276 2212 6 -0.15553603822286988 2212 7 -0.22110113557207 2212 8 -0.3624267979922706 2212 9 -0.14201433650517714 2212 10 -0.5767005879095848 2212 11 -0.18608100376454928 2212 12 -0.3243802514125491 2212 13 -0.20960160737253375 2212 14 -0.35162655125506603 2212 15 -0.1685557705006886 2212 16 -0.307706550277274 2212 17 -0.5113722946807755 2212 18 -0.3089413118353761 2212 19 -0.46134076684919023 2212 20 -0.643981892476468 2212 21 -0.055021032650067525 2212 22 -0.36416769665824233 2212 23 -0.3204568446311242 2212 24 -0.39772881508294944 2212 25 -0.25295869952488204 2213 1 -0.18160248069942853 2213 2 -0.16746149895255869 2213 3 -0.1272677561212916 2213 4 -0.06343816722633022 2213 5 -0.08140242728217117 2213 6 -0.22884696630516999 2213 7 -0.27594508773212567 2213 8 -0.3121214870010789 2213 9 -0.22485577990824623 2213 10 -0.1957095500099939 2213 11 -0.015001528236766148 2213 12 0.02888113266394754 2213 13 0.1304189833687346 2213 14 -0.5533803143905374 2213 15 -0.19707315823775673 2213 16 -0.3420418085687942 2213 17 -0.30674135018899307 2213 18 -0.14503427784881495 2213 19 -0.21232099658190862 2213 20 -0.3791068569751914 2213 21 0.06344909894133366 2213 22 -0.3383866685412258 2213 23 -0.4672861860429691 2213 24 -0.6773976276046483 2213 25 -0.45795057367679703 2214 1 -0.255854694688221 2214 2 -0.17127207704245961 2214 3 -0.21035075165458395 2214 4 -0.1427631911090046 2214 5 -0.11617931788659774 2214 6 -0.0421521998390304 2214 7 -0.23710943283036556 2214 8 -0.14838459020006667 2214 9 -0.2682228502136894 2214 10 -0.1354036903626863 2214 11 -0.08678446696801245 2214 12 -0.14862267565496978 2214 13 -0.19572173724266406 2214 14 -0.2073895382680533 2214 15 -0.16684220628712154 2214 16 -0.4287924088631314 2214 17 -0.45859451690299835 2214 18 -0.2837248616189214 2214 19 -0.4815968996053215 2214 20 -0.43513448987906145 2214 21 -0.5965524179314556 2214 22 -0.738274839515384 2214 23 -0.8109149975892791 2214 24 -0.5201765062806416 2214 25 -1.0892862608524467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 28.0 10 72.0 11 184.0 12 505.0 13 1329.0 14 2834.0 15 5262.0 16 8012.0 17 10589.0 18 12377.0 19 13230.0 20 13912.0 21 14771.0 22 15180.0 23 16029.0 24 17419.0 25 19529.0 26 21785.0 27 24732.0 28 28752.0 29 35540.0 30 45264.0 31 59336.0 32 78530.0 33 106536.0 34 145835.0 35 203489.0 36 289503.0 37 461032.0 38 982155.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.760161047462184 20.58550344813957 12.243422767830337 24.410912736567912 2 14.8339481713568 22.57236433443341 37.83463616219574 24.75905133201405 3 18.290770383623524 27.69219480500224 30.071198257511327 23.945836553862907 4 11.980241782978588 18.35153768688512 36.84509515325225 32.823125376884036 5 12.27665889916117 39.15920297494448 34.657974048227395 13.906164077666952 6 30.19004557190058 38.161197924676685 17.41586324404643 14.232893259376302 7 25.06551936414791 34.69015350576544 22.592854069454134 17.651473060632515 8 25.364608413374267 36.23287088586059 20.963331809226364 17.439188891538777 9 25.693619945666928 15.955432452904695 21.349518083496758 37.00142951793162 10 15.679218070606602 30.37946169936394 32.67179029022009 21.269529939809363 11 31.796560611770996 25.886821757261135 22.339458743146146 19.977158887821723 12 21.44497407091561 28.54598928816329 31.087411655444274 18.921624985476825 13 28.235455372479457 23.024000704697016 26.46106928660058 22.279474636222947 14 20.92871910044101 23.942803660960575 28.624557899712766 26.50391933888565 15 24.93207326519144 28.782140850204275 24.86380480840788 21.421981076196406 16 20.428499776174398 29.93089313438231 27.952912713331994 21.687694376111295 17 20.57871745853354 29.494467025216846 28.809589350566018 21.117226165683597 18 21.169931029769394 27.188888825607645 30.710680039259685 20.930500105363276 19 20.986352133558867 28.49953203518184 31.42217640659973 19.09193942465956 20 22.15737174850622 26.352832898782 30.862712534328416 20.627082818383364 21 21.35062173706692 27.494295206454677 30.879240626483153 20.275842429995254 22 21.395467337767172 27.131311121607602 29.6141070834618 21.859114457163425 23 20.597771011762312 27.44800096896024 30.74033953855167 21.213888480725778 24 21.030058236266562 28.230085269922707 29.898995881923675 20.840860611887063 25 21.324722929187804 26.492712000130613 29.785819487544767 22.396745583136813 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3305.0 1 3305.0 2 3676.5 3 4048.0 4 4048.0 5 4048.0 6 8737.5 7 13427.0 8 13427.0 9 13427.0 10 15113.0 11 16799.0 12 16799.0 13 16799.0 14 19840.5 15 22882.0 16 22882.0 17 22882.0 18 37558.0 19 52234.0 20 52234.0 21 52234.0 22 75604.0 23 98974.0 24 98974.0 25 98974.0 26 131126.0 27 163278.0 28 163278.0 29 163278.0 30 190149.5 31 217021.0 32 217021.0 33 217021.0 34 236533.0 35 256045.0 36 256045.0 37 256045.0 38 254771.5 39 253498.0 40 253498.0 41 253498.0 42 262736.0 43 271974.0 44 271974.0 45 271974.0 46 318928.0 47 365882.0 48 365882.0 49 365882.0 50 335539.0 51 305196.0 52 305196.0 53 305196.0 54 259284.0 55 213372.0 56 213372.0 57 213372.0 58 181604.0 59 149836.0 60 149836.0 61 149836.0 62 129957.0 63 110078.0 64 110078.0 65 110078.0 66 85874.0 67 61670.0 68 61670.0 69 61670.0 70 47297.0 71 32924.0 72 32924.0 73 32924.0 74 23366.0 75 13808.0 76 13808.0 77 13808.0 78 9484.0 79 5160.0 80 5160.0 81 5160.0 82 3381.0 83 1602.0 84 1602.0 85 1602.0 86 1083.5 87 565.0 88 565.0 89 565.0 90 358.5 91 152.0 92 152.0 93 152.0 94 86.5 95 21.0 96 21.0 97 21.0 98 13.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09423803875529851 2 0.0 3 0.13270021976219512 4 0.012149948590530027 5 0.021414284390809172 6 0.01271947743071112 7 0.021452252980154577 8 0.019743666459611293 9 3.796858934540633E-5 10 0.38317900367384067 11 0.06302785831337451 12 0.0012909320377438153 13 0.0 14 7.97340376253533E-4 15 0.001366869216434628 16 3.41717304108657E-4 17 3.796858934540633E-5 18 4.1765448279946967E-4 19 4.1765448279946967E-4 20 1.8984294672703167E-4 21 2.27811536072438E-4 22 0.002733738432869256 23 1.8984294672703167E-4 24 7.593717869081266E-5 25 0.0016326493418524722 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2633756.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.66566388133357 #Duplication Level Percentage of deduplicated Percentage of total 1 80.00339355576631 45.33445408597102 2 12.68151482994733 14.372129137198849 3 3.679375165789249 6.254827093138187 4 1.4004143385813932 3.174216328186131 5 0.6591008912290455 1.8674194783136242 6 0.36126959227824723 1.2282948771951347 7 0.2505637054232633 0.993885109866328 8 0.15650535959995995 0.7094784082174858 9 0.11463099647914544 0.5846077365182629 >10 0.6066868480802382 6.127899539472695 >50 0.04417812023405625 1.7404384960178705 >100 0.03332052878379794 3.8147256640260356 >500 0.003712938279412397 1.4541814222908538 >1k 0.004320509997857014 4.783336491975217 >5k 5.400637497321268E-4 2.0700682244905466 >10k+ 4.7255578101561085E-4 5.49003790712173 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 30338 1.1518910635609372 No Hit CCCATGTACTCTGCGTTGATACCAC 24286 0.9221051608425382 No Hit GGTATCAACGCAGAGTACTTTTTTT 22606 0.8583179307422556 No Hit GTACATGGGAAGCAGTGGTATCAAC 21847 0.8294997714290921 No Hit TATCAACGCAGAGTACTTTTTTTTT 21784 0.8271077503003316 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 12295 0.46682380600177087 No Hit GAGTACATGGGAAGCAGTGGTATCA 10360 0.3933545856184096 No Hit CATGTACTCTGCGTTGATACCACTG 9298 0.3530319437335881 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 8431 0.3201131767711208 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 6737 0.25579438642000246 No Hit GCGTTGATACCACTGCTTCCCATGT 6711 0.2548072030970219 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6452 0.24497333845656166 No Hit TATCAACGCAGAGTACATGGGAAGC 5745 0.2181295457893594 No Hit GTATCAACGCAGAGTACATGGGAAG 5598 0.21254816315558464 No Hit ACTCTGCGTTGATACCACTGCTTCC 5142 0.19523448641407937 No Hit GGTATCAACGCAGAGTACATGGGAA 4951 0.18798248584910676 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 4259 0.16170822202208557 No Hit GTACTGGTTCACTATCGGTCAGTCA 3852 0.1462550061585052 No Hit GCTTCCCATGTACTCTGCGTTGATA 3476 0.1319788165646324 No Hit GTTAATGATAGTGTGTCGAAACACA 3389 0.12867554929158206 No Hit GTGGTATCAACGCAGAGTACATGGG 3268 0.1240813499807879 No Hit CAGTGGTATCAACGCAGAGTACATG 3017 0.1145512340550909 No Hit ATCAACGCAGAGTACTTTTTTTTTT 2905 0.1102987520484054 No Hit GGTATCAACGCAGAGTACATGGGGG 2871 0.10900782001066157 No Hit GGATACCACGTGTCCCGCCCTACTC 2691 0.10217347392848844 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.796858934540633E-5 0.0 9 0.0 0.0 0.0 3.796858934540633E-5 0.0 10 0.0 0.0 0.0 3.796858934540633E-5 0.0 11 0.0 0.0 0.0 3.796858934540633E-5 0.0 12 0.0 0.0 0.0 3.796858934540633E-5 0.0 13 0.0 0.0 0.0 1.13905768036219E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACTCG 50 0.0015206515 13.27592 14 CACGTCA 100 1.8857681E-6 11.422513 9 CTCGTCG 60 0.005953934 11.064108 17 TACTCCG 130 3.169407E-8 10.984651 5 CAGTACT 210 0.0 10.880035 4 GACTCGT 75 0.002688727 10.114986 15 TCACGTC 85 6.4696645E-4 10.080033 8 ACTCACG 95 1.6102119E-4 10.021086 6 CGCCCTA 620 0.0 9.9424 16 TGTCCCG 630 0.0 9.9404 11 CCAGTAC 225 1.8189894E-12 9.710488 3 TTAGACT 140 1.0292206E-6 9.520031 4 GTATTAA 370 0.0 9.509519 1 GTACTGG 1250 0.0 9.509519 1 CCCCCGG 705 0.0 9.147225 18 GCACCGA 105 4.400442E-4 9.066696 6 GTATAGG 285 0.0 9.009018 1 TACTGGT 1195 0.0 8.982688 2 TAATCAG 170 1.6323429E-7 8.96003 5 ACGGTGA 85 0.0073102065 8.96003 8 >>END_MODULE