FastQCFastQC Report
Wed 25 May 2016
SRR1294611_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294611_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2858534
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC598952.0953047960947813No Hit
CCCATGTACTCTGCGTTGATACCAC550391.9254275093456996No Hit
GAGTACATGGGAAGCAGTGGTATCA276210.9662645258023869No Hit
CATGTACTCTGCGTTGATACCACTG203920.7133726588524048No Hit
GCGTTGATACCACTGCTTCCCATGT171760.6008674376446108No Hit
GTATCAACGCAGAGTACATGGGAAG161930.5664791812866316No Hit
ACGCAGAGTACATGGGAAGCAGTGG150110.525129314536752No Hit
GTATCAACGCAGAGTACTTTTTTTT129440.45281952217465316No Hit
TATCAACGCAGAGTACATGGGAAGC127380.4456130310151987No Hit
ACTCTGCGTTGATACCACTGCTTCC115580.4043331301989061No Hit
GGTATCAACGCAGAGTACATGGGAA109110.3816991506835322No Hit
GCTTCCCATGTACTCTGCGTTGATA105370.368615521102775No Hit
GGTATCAACGCAGAGTACTTTTTTT80940.2831521332263321No Hit
GTGGTATCAACGCAGAGTACATGGG80320.28098318928513705No Hit
TATCAACGCAGAGTACTTTTTTTTT79250.27724001183823593No Hit
CAGTGGTATCAACGCAGAGTACATG75960.2657306157631849No Hit
ATACCACTGCTTCCCATGTACTCTG61510.21518022874662326No Hit
CATGGGAAGCAGTGGTATCAACGCA58990.20636452111466927No Hit
GCAGTGGTATCAACGCAGAGTACAT56770.1985983024865193No Hit
GGGAAGCAGTGGTATCAACGCAGAG55000.19240631736407543No Hit
ACATGGGAAGCAGTGGTATCAACGC53780.1881383954152723No Hit
GTACTCTGCGTTGATACCACTGCTT51840.18135169985733945No Hit
CTGCTTCCCATGTACTCTGCGTTGA47240.16525953513234407No Hit
TACCACTGCTTCCCATGTACTCTGC45990.16088666428316054No Hit
GCAGAGTACATGGGAAGCAGTGGTA45570.15941737967783484No Hit
GTTGATACCACTGCTTCCCATGTAC43700.1528755648874563No Hit
AAGCAGTGGTATCAACGCAGAGTAC42580.14895747260658784No Hit
GATACCACTGCTTCCCATGTACTCT42100.14727829020050137No Hit
CTTCCCATGTACTCTGCGTTGATAC41930.14668357976501242No Hit
ATGGGAAGCAGTGGTATCAACGCAG39470.13807776993381923No Hit
ACGCAGAGTACTTTTTTTTTTTTTT39380.137762923232678No Hit
GTACATGGTAAGCAGTGGTATCAAC38750.1355589963246895No Hit
CCATGTACTCTGCGTTGATACCACT38090.13325012051632062No Hit
TTCCCATGTACTCTGCGTTGATACC34850.1219156392752369No Hit
CCACTGCTTCCCATGTACTCTGCGT34610.12107604807219365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG1051.378794E-913.567049
AGGACCG550.003076568512.0872115
TGGATCG550.003076568512.0872115
CCGCCCG550.00307697212.0869999
AGAGCCG6250.011.70267319
TAGACCC2300.011.1489584
TACACCG2050.011.1185495
AGAGCGC1803.6379788E-1211.0799436
AGTACGC5250.011.03684419
AAGAGCC6900.010.88339218
AAGTACG5350.010.83832918
GTGATCG803.7834406E-410.6842317
CGTCGTT1252.2295353E-710.64438512
TATACCG2700.010.5523275
CGCTAGA1451.4526449E-810.47918510
CCGCTCC1106.073182E-610.3602859
CCCCCGG2400.010.29789318
TACCGTT2600.010.227647
TAAGGCC1409.738869E-810.1756354
GACTGGT2350.010.10329157
Produced by FastQC (version 0.11.5)