##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294611_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2858534 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.771561226838653 31.0 30.0 33.0 25.0 34.0 2 29.92682647818777 31.0 30.0 34.0 25.0 34.0 3 29.920526045868268 31.0 30.0 34.0 25.0 34.0 4 33.42126663527529 35.0 35.0 37.0 26.0 37.0 5 33.93096216452209 35.0 35.0 37.0 28.0 37.0 6 33.91909384320774 37.0 35.0 37.0 28.0 37.0 7 33.897188558890676 37.0 35.0 37.0 28.0 37.0 8 33.67789433324914 37.0 35.0 37.0 27.0 37.0 9 34.739076393703904 39.0 35.0 39.0 25.0 39.0 10 34.95558107757333 39.0 35.0 39.0 27.0 39.0 11 35.14116886487969 39.0 35.0 39.0 27.0 39.0 12 34.975411871959544 39.0 35.0 39.0 27.0 39.0 13 34.74616079430925 38.0 35.0 39.0 26.0 39.0 14 35.67628476694697 39.0 35.0 41.0 25.0 41.0 15 35.653838296133614 39.0 34.0 41.0 25.0 41.0 16 35.73028167585203 39.0 34.0 41.0 26.0 41.0 17 35.80026055313668 39.0 35.0 41.0 27.0 41.0 18 35.698143873747874 39.0 34.0 41.0 26.0 41.0 19 35.941948215413916 39.0 34.0 41.0 27.0 41.0 20 36.10386093011313 39.0 35.0 41.0 27.0 41.0 21 36.15017418019166 39.0 35.0 41.0 27.0 41.0 22 36.22456615873731 39.0 35.0 41.0 27.0 41.0 23 36.23596570829663 39.0 35.0 41.0 27.0 41.0 24 36.27102983557306 39.0 35.0 41.0 27.0 41.0 25 35.168160672568526 39.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9696386471885496 1101 2 0.9825691144577888 1101 3 1.2164771422624874 1101 4 1.0530160604915295 1101 5 0.850270553346725 1101 6 0.7040263340233466 1101 7 0.7007990069094205 1101 8 0.8644847004597551 1101 9 1.1068039053964682 1101 10 1.0868469834107017 1101 11 0.7749759984788085 1101 12 0.9589265492012657 1101 13 1.0715864805587074 1101 14 1.1399739393017612 1101 15 1.2852059340502962 1101 16 1.3088342708654466 1101 17 1.216888173078594 1101 18 1.1925593572927724 1101 19 1.1532214333781425 1101 20 1.009004298532858 1101 21 0.9774389631963629 1101 22 0.9441866022826346 1101 23 0.862006214276434 1101 24 0.997819062067073 1101 25 1.0184362656357848 1102 1 0.8737584896361099 1102 2 0.908368878046069 1102 3 1.1271454617244352 1102 4 1.0182602555242113 1102 5 0.713991929907607 1102 6 0.6812917884848133 1102 7 0.6348079441193093 1102 8 0.7315645456869646 1102 9 1.1464239893916144 1102 10 0.8581059310290655 1102 11 0.748666001711257 1102 12 1.0474912946403023 1102 13 1.0282035420941327 1102 14 1.2411358292624257 1102 15 1.2001977982171823 1102 16 1.1565584003292813 1102 17 1.1940296375042436 1102 18 1.230338736270852 1102 19 1.0703001592508627 1102 20 0.3003238513461852 1102 21 0.5564825603823138 1102 22 0.5633078512674956 1102 23 0.695969374446129 1102 24 0.836356684685768 1102 25 0.7776380939620537 1103 1 0.664411827361036 1103 2 0.7137940226017676 1103 3 0.9595939860683309 1103 4 0.7304876666802755 1103 5 0.5711135680233923 1103 6 0.46324154421513697 1103 7 0.46630740578712704 1103 8 0.5185173339699105 1103 9 0.5803709479481398 1103 10 0.6116714571600852 1103 11 0.5206641343793734 1103 12 0.7394225913984442 1103 13 0.600575181392351 1103 14 0.48699659871869017 1103 15 0.7970438026089752 1103 16 0.7630102553991236 1103 17 0.7120054239779208 1103 18 0.8204384557786 1103 19 0.7310221774747561 1103 20 -0.022634800978778458 1103 21 0.231184309058726 1103 22 -0.20325171250381402 1103 23 0.31800664802484846 1103 24 0.5240098029317721 1103 25 0.5612810877994363 1104 1 0.29377598075838307 1104 2 0.28753001107077125 1104 3 0.5115957054511959 1104 4 0.32239454774121157 1104 5 0.20312193995435734 1104 6 0.15403415667270082 1104 7 0.07814623203805127 1104 8 0.18023904834709725 1104 9 0.5490260445068742 1104 10 0.46773400333089654 1104 11 0.1466164496610105 1104 12 0.325073832726531 1104 13 0.1873830483591803 1104 14 0.5260893500012145 1104 15 0.5112407605988807 1104 16 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0.3686575188773844 2207 7 0.26224639276693296 2207 8 0.2601361667941333 2207 9 -1.2350081045348347 2207 10 -0.14917867431877596 2207 11 0.1104186286744735 2207 12 0.3200499760776765 2207 13 0.5127167989608736 2207 14 0.6879867952108043 2207 15 0.6185245680073521 2207 16 0.6695023670623641 2207 17 0.6255901720187751 2207 18 0.675083691998843 2207 19 -0.523292160920029 2207 20 0.16701807553918968 2207 21 -0.480977821709601 2207 22 0.08118972080940523 2207 23 -0.5338212945881367 2207 24 -1.5036143339295123 2207 25 -0.2404514083804159 2208 1 0.40862462565784696 2208 2 0.08369821197456773 2208 3 0.6563853510831841 2208 4 0.4127763153762558 2208 5 0.3219630988005022 2208 6 0.14811564079862194 2208 7 0.28066824627345 2208 8 0.4035216859719739 2208 9 -0.15809197529953423 2208 10 0.127874805359383 2208 11 0.2663617075155855 2208 12 0.22340363090288662 2208 13 0.4390349074558486 2208 14 0.7698989057728625 2208 15 0.7154077877677594 2208 16 0.4432220848674788 2208 17 0.4472583177907623 2208 18 0.591466458526547 2208 19 -1.4247061935272498 2208 20 -0.17907214285870765 2208 21 -0.4092492102703247 2208 22 0.020638395062903214 2208 23 0.22201050248474985 2208 24 0.34198179698036313 2208 25 0.43015203393166246 2209 1 0.26122040722842854 2209 2 -1.2784551276376348 2209 3 -0.13975592667039294 2209 4 0.26483426834358426 2209 5 0.26890857461286544 2209 6 0.22562583931381397 2209 7 0.3134279194914882 2209 8 0.4486660988691611 2209 9 -1.9242890753495345 2209 10 -0.21212802185191038 2209 11 0.23420532298614205 2209 12 0.47127694929498887 2209 13 0.5705985267395164 2209 14 0.8265678825744871 2209 15 0.8502416529185624 2209 16 0.8199341982933461 2209 17 0.7133148877617828 2209 18 0.8519241891372076 2209 19 0.56334649558314 2209 20 0.5042159477776806 2209 21 0.5078334457968126 2209 22 0.4774001549360918 2209 23 0.4604835509333398 2209 24 0.5678122695369296 2209 25 0.5995917128041128 2210 1 0.15112524568067087 2210 2 0.13352670976033565 2210 3 0.07661412472887008 2210 4 0.22681332476916083 2210 5 0.06574466588247674 2210 6 0.09304945735177483 2210 7 0.17471456132826546 2210 8 0.14597508023343408 2210 9 0.3601712727973094 2210 10 -1.5668367132106837 2210 11 -0.47977074496370875 2210 12 -2.1072729233447944 2210 13 -0.650846710150006 2210 14 -0.4372104095115219 2210 15 -0.6332078177043243 2210 16 -0.08158532977801514 2210 17 0.060563026441506906 2210 18 0.27735054251109403 2210 19 0.25075465070150216 2210 20 0.1818508270187067 2210 21 0.21188646349057194 2210 22 0.22710262654313595 2210 23 0.17937332376473591 2210 24 0.19930059604875794 2210 25 0.10867173623559268 2211 1 -0.3220762916799629 2211 2 -0.08872948882826392 2211 3 -1.5305327246703442 2211 4 -0.7010168319448908 2211 5 -0.5341637253785407 2211 6 -0.4450698497417349 2211 7 -0.37924879890638863 2211 8 -0.060074888960912176 2211 9 0.04781410254059182 2211 10 0.08806667018883019 2211 11 -0.27766287357529507 2211 12 -0.38693136916072746 2211 13 -0.24850787407525132 2211 14 -0.7395581981878436 2211 15 -0.18528180598263333 2211 16 -0.058220718292950835 2211 17 -0.1327192894970466 2211 18 -0.09729901598265656 2211 19 -0.19758047626068986 2211 20 -0.12482834220473649 2211 21 -0.2074310747180803 2211 22 -0.2303928147797123 2211 23 -0.3421573410716192 2211 24 -0.2803040409014912 2211 25 -0.23408069759753403 2212 1 -0.39454712283085414 2212 2 -0.3186415078364071 2212 3 -2.2616236434226664 2212 4 -1.270612908858034 2212 5 -0.5959098330356909 2212 6 -0.2696407621175254 2212 7 -0.17611822418327705 2212 8 -0.27033503638030965 2212 9 -0.7348766431343776 2212 10 -0.1506085288880925 2212 11 -0.18752600582006096 2212 12 0.001692589907996478 2212 13 -0.25781002311914847 2212 14 0.09337356530345886 2212 15 0.24723092810828717 2212 16 -1.0155525941327141 2212 17 -1.2538977337744868 2212 18 -0.22624924105913635 2212 19 -0.08443494704629018 2212 20 -0.17224546623592119 2212 21 -0.03412446210013087 2212 22 -0.34145230632700674 2212 23 -0.10967952609738774 2212 24 -0.20051841391356362 2212 25 -0.08703022429168072 2213 1 -3.0372470098031847 2213 2 -1.4533164304370274 2213 3 -2.26572826772853 2213 4 -1.085044821974158 2213 5 -0.8454626366132629 2213 6 -0.5970426000456754 2213 7 -0.43397487561577464 2213 8 -0.1991128633005559 2213 9 -0.42889477670359355 2213 10 -0.31838458667962755 2213 11 -0.5940508964780804 2213 12 -0.3834356719230527 2213 13 -0.73774638362827 2213 14 -0.30403171259717965 2213 15 -0.3626233644859411 2213 16 -1.5294979968552056 2213 17 -1.0400248161863956 2213 18 -0.41466390517039997 2213 19 -0.23318795702220996 2213 20 -0.21987065666296957 2213 21 -0.1431900181471093 2213 22 -0.08182406906718853 2213 23 -0.14876498484229472 2213 24 -0.12117790578049181 2213 25 -0.16121323082762729 2214 1 -0.8283294582693834 2214 2 -0.6525769042503882 2214 3 -6.30074219350638 2214 4 -1.9552721546823335 2214 5 -1.1514116952194513 2214 6 -0.6073139842567628 2214 7 -0.45443467897031553 2214 8 -0.3587107003127983 2214 9 -0.14991357650369252 2214 10 -0.12461061665374018 2214 11 -0.5863112804477097 2214 12 -0.5012740037496286 2214 13 -5.150895153611476 2214 14 -3.58467937357187 2214 15 -2.152099996693792 2214 16 -2.7835927651426147 2214 17 -3.459732599460864 2214 18 -1.0114281271517882 2214 19 -0.5153195836990605 2214 20 -0.2592274036461859 2214 21 -0.2529592601468096 2214 22 -0.23499878142688146 2214 23 -0.16925525083588155 2214 24 0.0013054871679045732 2214 25 -0.013475046681676872 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1130.0 3 133.0 4 150.0 5 303.0 6 36.0 7 43.0 8 74.0 9 231.0 10 566.0 11 1520.0 12 3455.0 13 6704.0 14 11316.0 15 17770.0 16 23413.0 17 26879.0 18 28608.0 19 27896.0 20 27373.0 21 26023.0 22 25036.0 23 24544.0 24 24617.0 25 25184.0 26 27064.0 27 29960.0 28 34681.0 29 41934.0 30 52002.0 31 65465.0 32 86177.0 33 115269.0 34 157059.0 35 217196.0 36 309059.0 37 475011.0 38 944653.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.75453268558754 21.788022118980393 13.112054816299073 21.345390379132994 2 13.314311055543907 22.642939221932366 40.67821467335853 23.36453504916519 3 19.163063981655622 30.534212468982957 28.84563216664794 21.457091382713482 4 11.484900318405684 20.358958043042342 37.17341716814599 30.98272447040598 5 10.407267692355077 42.84325783353832 34.442158551669536 12.307315922437068 6 27.596837025896953 38.55655349526282 19.641441850174616 14.205167628665608 7 23.840457089088545 36.89845506296457 22.650266777657748 16.610821070289138 8 25.053548406744564 37.77330340199968 20.790118599350805 16.383029591904958 9 25.939077907129793 19.236849548699308 21.461427303201813 33.36264524096909 10 15.546550314218821 32.60527870817417 31.47705250422879 20.371118473378218 11 28.567208064967797 30.049075889106692 21.57308877065248 19.81062727527303 12 21.295091556166035 31.90574253239059 29.480661908147344 17.318504003296027 13 26.507743980453206 25.40487654612696 25.773409154156756 22.313970319263074 14 20.11623913911766 27.174110519946638 27.203060037518856 25.50659030341685 15 26.18432337630968 29.89105968263081 23.541715529946337 20.38290141111317 16 18.52549225518509 32.52601732738252 29.080738601557716 19.867751815874684 17 21.219895724158402 31.88223004956148 28.469041052924155 18.42883317335596 18 21.84111482584262 29.677259782350134 29.934476620488855 18.547148771318394 19 20.474448772919366 31.940749215133295 30.889475924686405 16.695326087260934 20 22.268312909324685 29.24716638539679 29.125837027297706 19.358683677980824 21 20.232390279009753 29.894502346375912 30.989594454669756 18.883512919944582 22 20.851144419539168 29.629969323682236 26.923501041972607 22.595385214805987 23 18.718168181571546 30.353162746817475 29.64890263476177 21.279766436849208 24 20.082861980257086 32.77932133559452 27.742308624115818 19.395508060032572 25 20.555525993721066 28.226460300167755 27.290816569439347 23.92719713667183 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4952.0 1 4952.0 2 5596.0 3 6240.0 4 6240.0 5 6240.0 6 9018.0 7 11796.0 8 11796.0 9 11796.0 10 15812.0 11 19828.0 12 19828.0 13 19828.0 14 27510.0 15 35192.0 16 35192.0 17 35192.0 18 51419.0 19 67646.0 20 67646.0 21 67646.0 22 93618.5 23 119591.0 24 119591.0 25 119591.0 26 155067.5 27 190544.0 28 190544.0 29 190544.0 30 221106.5 31 251669.0 32 251669.0 33 251669.0 34 270073.0 35 288477.0 36 288477.0 37 288477.0 38 293300.5 39 298124.0 40 298124.0 41 298124.0 42 304990.5 43 311857.0 44 311857.0 45 311857.0 46 405848.0 47 499839.0 48 499839.0 49 499839.0 50 429494.0 51 359149.0 52 359149.0 53 359149.0 54 270600.5 55 182052.0 56 182052.0 57 182052.0 58 140624.5 59 99197.0 60 99197.0 61 99197.0 62 79864.5 63 60532.0 64 60532.0 65 60532.0 66 45084.0 67 29636.0 68 29636.0 69 29636.0 70 21811.5 71 13987.0 72 13987.0 73 13987.0 74 9680.5 75 5374.0 76 5374.0 77 5374.0 78 3670.5 79 1967.0 80 1967.0 81 1967.0 82 1288.0 83 609.0 84 609.0 85 609.0 86 397.5 87 186.0 88 186.0 89 186.0 90 120.5 91 55.0 92 55.0 93 55.0 94 35.5 95 16.0 96 16.0 97 16.0 98 17.5 99 19.0 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10099582513274287 2 0.053628888094386845 3 0.05726711664090754 4 0.05201967162188731 5 0.04883622164368169 6 0.06933624018465409 7 0.06464852263432934 8 0.06202480012481923 9 0.06653760284117663 10 0.06737719404421987 11 0.060660464419873966 12 0.06167497045688454 13 0.0629343572614494 14 0.0642637099996012 15 0.06730722811063293 16 0.06240961275954738 17 0.06576797757172033 18 0.1445846017574043 19 0.06989596765334959 20 0.06461353966753587 21 0.06254954462672126 22 0.06762207481177414 23 0.06713231327666559 24 0.0775922203479126 25 0.06989596765334959 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2858534.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.426734767987014 #Duplication Level Percentage of deduplicated Percentage of total 1 83.43582376886101 43.74267802878583 2 10.112444990482329 10.60324942743752 3 2.809686496417816 4.419080661866746 4 1.1182501874951063 2.345048239362308 5 0.567346052764466 1.4872050514973514 6 0.35223530915594975 1.1079928277423332 7 0.25926277431817996 0.9514610493074187 8 0.18773678602921534 0.7873941349878403 9 0.13619296726281802 0.642613731475761 >10 0.8953015081959191 8.970413397725618 >50 0.07567887339442937 2.712923927156282 >100 0.0396663312880147 3.9561396095209673 >500 0.004782797128357004 1.7663497213265933 >1k 0.004109163729994591 4.660528660897093 >5k 6.736333983597689E-4 2.3109639181181536 >10k+ 8.083600780317227E-4 9.535957612792242 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 59895 2.0953047960947813 No Hit CCCATGTACTCTGCGTTGATACCAC 55039 1.9254275093456996 No Hit GAGTACATGGGAAGCAGTGGTATCA 27621 0.9662645258023869 No Hit CATGTACTCTGCGTTGATACCACTG 20392 0.7133726588524048 No Hit GCGTTGATACCACTGCTTCCCATGT 17176 0.6008674376446108 No Hit GTATCAACGCAGAGTACATGGGAAG 16193 0.5664791812866316 No Hit ACGCAGAGTACATGGGAAGCAGTGG 15011 0.525129314536752 No Hit GTATCAACGCAGAGTACTTTTTTTT 12944 0.45281952217465316 No Hit TATCAACGCAGAGTACATGGGAAGC 12738 0.4456130310151987 No Hit ACTCTGCGTTGATACCACTGCTTCC 11558 0.4043331301989061 No Hit GGTATCAACGCAGAGTACATGGGAA 10911 0.3816991506835322 No Hit GCTTCCCATGTACTCTGCGTTGATA 10537 0.368615521102775 No Hit GGTATCAACGCAGAGTACTTTTTTT 8094 0.2831521332263321 No Hit GTGGTATCAACGCAGAGTACATGGG 8032 0.28098318928513705 No Hit TATCAACGCAGAGTACTTTTTTTTT 7925 0.27724001183823593 No Hit CAGTGGTATCAACGCAGAGTACATG 7596 0.2657306157631849 No Hit ATACCACTGCTTCCCATGTACTCTG 6151 0.21518022874662326 No Hit CATGGGAAGCAGTGGTATCAACGCA 5899 0.20636452111466927 No Hit GCAGTGGTATCAACGCAGAGTACAT 5677 0.1985983024865193 No Hit GGGAAGCAGTGGTATCAACGCAGAG 5500 0.19240631736407543 No Hit ACATGGGAAGCAGTGGTATCAACGC 5378 0.1881383954152723 No Hit GTACTCTGCGTTGATACCACTGCTT 5184 0.18135169985733945 No Hit CTGCTTCCCATGTACTCTGCGTTGA 4724 0.16525953513234407 No Hit TACCACTGCTTCCCATGTACTCTGC 4599 0.16088666428316054 No Hit GCAGAGTACATGGGAAGCAGTGGTA 4557 0.15941737967783484 No Hit GTTGATACCACTGCTTCCCATGTAC 4370 0.1528755648874563 No Hit AAGCAGTGGTATCAACGCAGAGTAC 4258 0.14895747260658784 No Hit GATACCACTGCTTCCCATGTACTCT 4210 0.14727829020050137 No Hit CTTCCCATGTACTCTGCGTTGATAC 4193 0.14668357976501242 No Hit ATGGGAAGCAGTGGTATCAACGCAG 3947 0.13807776993381923 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3938 0.137762923232678 No Hit GTACATGGTAAGCAGTGGTATCAAC 3875 0.1355589963246895 No Hit CCATGTACTCTGCGTTGATACCACT 3809 0.13325012051632062 No Hit TTCCCATGTACTCTGCGTTGATACC 3485 0.1219156392752369 No Hit CCACTGCTTCCCATGTACTCTGCGT 3461 0.12107604807219365 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.498296679346826E-5 0.0 10 0.0 0.0 0.0 6.996593358693652E-5 0.0 11 0.0 0.0 0.0 6.996593358693652E-5 0.0 12 0.0 0.0 0.0 1.0494890038040478E-4 0.0 13 0.0 0.0 0.0 1.0494890038040478E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCTAG 105 1.378794E-9 13.56704 9 AGGACCG 55 0.0030765685 12.087211 5 TGGATCG 55 0.0030765685 12.087211 5 CCGCCCG 55 0.003076972 12.086999 9 AGAGCCG 625 0.0 11.702673 19 TAGACCC 230 0.0 11.148958 4 TACACCG 205 0.0 11.118549 5 AGAGCGC 180 3.6379788E-12 11.079943 6 AGTACGC 525 0.0 11.036844 19 AAGAGCC 690 0.0 10.883392 18 AAGTACG 535 0.0 10.838329 18 GTGATCG 80 3.7834406E-4 10.684231 7 CGTCGTT 125 2.2295353E-7 10.644385 12 TATACCG 270 0.0 10.552327 5 CGCTAGA 145 1.4526449E-8 10.479185 10 CCGCTCC 110 6.073182E-6 10.360285 9 CCCCCGG 240 0.0 10.297893 18 TACCGTT 260 0.0 10.22764 7 TAAGGCC 140 9.738869E-8 10.175635 4 GACTGGT 235 0.0 10.1032915 7 >>END_MODULE