FastQCFastQC Report
Wed 25 May 2016
SRR1294611_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294611_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2858534
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC610262.134870531538194No Hit
GTACATGGGAAGCAGTGGTATCAAC605842.119408060215481No Hit
GTATCAACGCAGAGTACTTTTTTTT317031.1090649962533243No Hit
GAGTACATGGGAAGCAGTGGTATCA294361.0297586105325318No Hit
CATGTACTCTGCGTTGATACCACTG225940.7904051517316218No Hit
GGTATCAACGCAGAGTACTTTTTTT214750.7512592118897309No Hit
TATCAACGCAGAGTACTTTTTTTTT198010.692697725477465No Hit
ACGCAGAGTACATGGGAAGCAGTGG179670.6285389643782442No Hit
GCGTTGATACCACTGCTTCCCATGT178750.6253205314332452No Hit
GTATCAACGCAGAGTACATGGGAAG166460.5823264652440726No Hit
TATCAACGCAGAGTACATGGGAAGC135470.4739142511511145No Hit
ACTCTGCGTTGATACCACTGCTTCC128550.4497060381300345No Hit
ACGCAGAGTACTTTTTTTTTTTTTT126870.44382889970873185No Hit
GGTATCAACGCAGAGTACATGGGAA116430.407306682376351No Hit
GCTTCCCATGTACTCTGCGTTGATA111680.3906897731494535No Hit
CAGTGGTATCAACGCAGAGTACATG91460.3199542142930607No Hit
GTGGTATCAACGCAGAGTACATGGG87520.3061709253764342No Hit
CATGGGAAGCAGTGGTATCAACGCA76010.2659055305971522No Hit
ACATGGGAAGCAGTGGTATCAACGC68250.23875874836542088No Hit
GCAGTGGTATCAACGCAGAGTACAT67640.2366247873910193No Hit
GGGAAGCAGTGGTATCAACGCAGAG65900.23053775116895583No Hit
ATACCACTGCTTCCCATGTACTCTG64800.22668962482167432No Hit
GTACTTTTTTTTTTTTTTTTTTTTT61450.2149703309458625No Hit
GAGTACTTTTTTTTTTTTTTTTTTT60060.21010769856157036No Hit
GCAGAGTACATGGGAAGCAGTGGTA53990.18887303771793515No Hit
CTGCTTCCCATGTACTCTGCGTTGA51840.18135169985733945No Hit
AAGCAGTGGTATCAACGCAGAGTAC49870.1744600553990262No Hit
GTACTCTGCGTTGATACCACTGCTT49740.17400527683071113No Hit
GATACCACTGCTTCCCATGTACTCT48790.17068189498533165No Hit
TACCACTGCTTCCCATGTACTCTGC47970.16781329170826725No Hit
ATGGGAAGCAGTGGTATCAACGCAG47470.16606414336859382No Hit
CTTCCCATGTACTCTGCGTTGATAC47470.16606414336859382No Hit
CCATGTACTCTGCGTTGATACCACT43930.15368017312370608No Hit
GTTGATACCACTGCTTCCCATGTAC40350.14115627101164444No Hit
CCACTGCTTCCCATGTACTCTGCGT39660.13874244630289512No Hit
GTACATGGTAAGCAGTGGTATCAAC37350.13066138097360397No Hit
ATCAACGCAGAGTACTTTTTTTTTT35090.12275523047828013No Hit
ATCAACGCAGAGTACATGGGAAGCA33080.115723654152793No Hit
TTCCCATGTACTCTGCGTTGATACC32930.11519890965089098No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA31090.10876204376089281No Hit
GTGGTATCAACGCAGAGTACTTTTT30090.10526374708154598No Hit
ACCATGTACTCTGCGTTGATACCAC29030.10155555260143835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG456.6514604E-414.810745
ACGCGGG604.157255E-412.64362318
TCAGGAC2250.011.819533
CGTCGTT1251.8835635E-811.3794612
GTCTCGC600.00584176211.0924691
CGCGAAC600.005957122411.06336312
CCCCGTA3200.010.96439215
TAGGGCC700.001464585910.8813594
AGGGCCG700.001464585910.8813595
TATCGGG1158.582574E-710.76232810
CCGTCTA1605.2568794E-1010.7105879
GCGCTAG803.6982715E-410.7105879
ACACCGT2950.010.6508236
TAGGACC1553.5342964E-910.44259554
ACTGATC1658.949428E-1010.3867538
ATCGGGT1106.141494E-610.35057711
AAGTACG3850.010.34478318
TCGCGCG2700.010.2257459
CACACCG750.002600220710.1559365
GTATAGG3100.010.121241
Produced by FastQC (version 0.11.5)