FastQCFastQC Report
Wed 25 May 2016
SRR1294610_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294610_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145115
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18491.2741618716190608No Hit
GGTATCAACGCAGAGTACTTTTTTT12560.865520449298832No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA12410.8551838197291803No Hit
TATCAACGCAGAGTACTTTTTTTTT10740.7401026771870586No Hit
GTACTGGTTCACTATCGGTCAGTCA8800.6064156014195637No Hit
ATACAGGGTGACAGCCCCGTACACA6480.4465423974089515No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5700.39279192364676296No Hit
GGATACCACGTGTCCCGCCCTACTC5510.37969885952520416No Hit
ACCCTGTATCGCGCGCCTTTCCAGA3490.24049891465389517No Hit
GTTCACTATCGGTCAGTCAGGAGTA3240.22327119870447576No Hit
GTATTTAGCCTTGGAGGATGGTCCC2840.19570685318540468No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG2660.1833028977018227No Hit
GGTATCAACGCAGAGTACATGGGGG2500.17227715949419425No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2490.1715880508562175No Hit
GCCTTGGAGGATGGTCCCCCCATAT2430.16745339902835682No Hit
CCCATATTCAGACAGGATACCACGT2360.16262963856251939No Hit
GCCCAGAGCCTGAATCAGTGTGTGT2300.1584949867346587No Hit
GTCAGGAGTATTTAGCCTTGGAGGA2260.15573855218275162No Hit
TATTCAGACAGGATACCACGTGTCC2250.15504944354477482No Hit
TCACGGTACTGGTTCACTATCGGTC2240.15436033490679807No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2230.15367122626882126No Hit
CATCTAAGTACCCCGAGGAAAAGAA2150.14815835716500705No Hit
CTATCGGTCAGTCAGGAGTATTTAG2110.14540192261309995No Hit
GGTTAATGAGGCGAACCGGGGGAAC1980.13644351031940186No Hit
CGGTACTGGTTCACTATCGGTCAGT1930.13299796712951797No Hit
ATCATTAACTGAATCCATAGGTTAA1920.1323088584915412No Hit
GTTAATGATAGTGTGTCGAAACACA1910.1316197498535644No Hit
TCTAAGTACCCCGAGGAAAAGAAAT1910.1316197498535644No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1900.13093064121558765No Hit
CACACACACTGATTCAGGCTCTGGG1850.12748509802570376No Hit
ATCAACGCAGAGTACTTTTTTTTTT1800.12403955483581987No Hit
GGTATCAACGCAGAGTACATGGGGA1690.11645935981807531No Hit
ACTTAGATGTTTCAGTTCCCCCGGT1680.11577025118009855No Hit
GCCCTACTCATCGAGCTCACAGCAT1660.114392033904145No Hit
GGTTCACTATCGGTCAGTCAGGAGT1650.11370292526616821No Hit
GTGGTATCAACGCAGAGTACTTTTT1650.11370292526616821No Hit
GTGGTATCAACGCAGAGTACATGGG1610.11094649071426112No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA1590.10956827343830755No Hit
AAACAAAAAAAAAAAAAAAAAAAAA1530.10543362161044689No Hit
GTACTTAGATGTTTCAGTTCCCCCG1480.10198807842056301No Hit
GGGTGACAGCCCCGTACACAAAAAT1470.10129896978258622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAAAG250.00600253919.002072
GGTATCA2600.014.6169761
CATCTAA500.00148692313.3014491
GTACTGG1950.012.6680471
GGGTGAC1207.166818E-1012.6636796
GGTGACA1301.9099389E-1012.4201477
ATCTAAG550.003042958712.0922262
TACTGGT1900.012.0013072
ACTGGTT1900.012.0013073
ACAGGGT1353.5652192E-1011.9642663
AGGGTGA1302.5320332E-911.6895495
TGGTTCA2050.011.5826335
CTGGTTC2100.011.3107554
ATACAGG1354.5529305E-911.2604861
GTGACAG1354.5765773E-911.2566038
TACAGGG1451.1659722E-911.1391442
GGTTCAC2150.011.0439066
AGCCCCG1408.041752E-910.85832613
TGACAGC1408.085408E-910.8545839
CAGCCCC1502.044544E-910.76783912
Produced by FastQC (version 0.11.5)