##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294610_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 145115 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.587423767356924 31.0 28.0 33.0 16.0 34.0 2 28.58969782586225 31.0 28.0 33.0 16.0 34.0 3 28.62555214829618 31.0 28.0 34.0 16.0 34.0 4 31.73448644178755 35.0 32.0 37.0 17.0 37.0 5 32.591689349826 35.0 32.0 37.0 22.0 37.0 6 32.4699583089274 35.0 32.0 37.0 19.0 37.0 7 32.418454329325016 35.0 32.0 37.0 18.0 37.0 8 32.216428349929366 35.0 32.0 37.0 17.0 37.0 9 32.83798366812528 37.0 32.0 39.0 15.0 39.0 10 33.1626365296489 37.0 32.0 39.0 17.0 39.0 11 33.34762774351376 37.0 32.0 39.0 17.0 39.0 12 33.239024222168624 37.0 32.0 39.0 17.0 39.0 13 32.956503462770904 37.0 32.0 39.0 16.0 39.0 14 33.63890707370017 38.0 31.0 40.0 16.0 41.0 15 33.70049960376253 38.0 31.0 40.0 17.0 41.0 16 33.91048478792682 38.0 32.0 40.0 17.0 41.0 17 34.00866209557937 38.0 32.0 40.0 17.0 41.0 18 33.97679082107294 38.0 32.0 40.0 17.0 41.0 19 34.27784171174586 39.0 32.0 40.0 18.0 41.0 20 34.417723874168765 39.0 32.0 41.0 18.0 41.0 21 34.49356717086449 39.0 32.0 41.0 19.0 41.0 22 34.610267718705856 39.0 32.0 41.0 21.0 41.0 23 34.63187816559281 38.0 32.0 41.0 22.0 41.0 24 34.6800055128691 38.0 32.0 41.0 23.0 41.0 25 33.590517865141436 38.0 30.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.27050574626974466 1101 2 0.16016082294408918 1101 3 0.3502597784716315 1101 4 -0.24533558581687132 1101 5 -0.1599371158249454 1101 6 -0.3279693616210153 1101 7 -0.4330156869897408 1101 8 0.0979354095093754 1101 9 -0.4723774613091436 1101 10 -0.12016245280894111 1101 11 -0.18193282936980637 1101 12 -0.10741225617589123 1101 13 -0.5920596080549814 1101 14 -0.2971856626983893 1101 15 -0.049178020878549944 1101 16 -0.27207608987625775 1101 17 0.0917754156012407 1101 18 -0.9104960035462071 1101 19 -0.2621200544553446 1101 20 -0.2911958412089888 1101 21 0.21221563202290383 1101 22 -0.1257167232671179 1101 23 -0.1661897306147253 1101 24 -0.04837139435098692 1101 25 -0.47777679406370765 1102 1 0.8404281976091283 1102 2 1.1012459041003098 1102 3 1.114163999140235 1102 4 0.6905980173557857 1102 5 0.09228088164019965 1102 6 0.6085775127401618 1102 7 0.8876297468655565 1102 8 0.881518821205141 1102 9 0.826776224450704 1102 10 1.1390877209512311 1102 11 0.9431472365909102 1102 12 1.0921870733142782 1102 13 0.6937520222924007 1102 14 1.09712045502242 1102 15 1.217976537089207 1102 16 0.9753175075725338 1102 17 0.9801055462961443 1102 18 1.2588077292310942 1102 19 0.9556457304636297 1102 20 0.5131706496532757 1102 21 0.7280857275569304 1102 22 0.620130067961739 1102 23 0.5944300821331439 1102 24 0.9701630237909384 1102 25 1.178982135846212 1103 1 0.9600041908982284 1103 2 1.030438308584408 1103 3 1.3379495554583904 1103 4 1.3533754138325627 1103 5 0.021358895824597823 1103 6 0.8563469018959609 1103 7 0.8213596332378543 1103 8 0.260341363720201 1103 9 0.7560830192345023 1103 10 0.45857982802054664 1103 11 0.687980608054346 1103 12 0.7942003425890434 1103 13 1.1701494904537668 1103 14 1.2527293927178391 1103 15 1.3039217822657463 1103 16 1.6043497656370533 1103 17 1.0940001547000193 1103 18 1.422500248105493 1103 19 1.0772807491473841 1103 20 -0.08509061779124494 1103 21 0.3606259548123276 1103 22 -0.11292110263232757 1103 23 0.7407352754527494 1103 24 0.3209218127789626 1103 25 1.0608932164532376 1104 1 -0.2953149580379417 1104 2 -0.30289502474892416 1104 3 -0.4634688842579209 1104 4 0.059049172697811514 1104 5 0.2518553497253109 1104 6 0.06911285934276634 1104 7 0.40291991692579643 1104 8 0.15750448428757835 1104 9 -0.08079641338961352 1104 10 -0.4421293918376108 1104 11 -0.05670024300948029 1104 12 -0.43984221060244266 1104 13 -0.10290015493630023 1104 14 0.2598215912993993 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-0.8426893022519408 2207 11 -0.35128777567092584 2207 12 -0.020320000822678708 2207 13 0.41897367858366863 2207 14 0.09979919488282718 2207 15 -0.18036339877195218 2207 16 -0.14126426945066584 2207 17 -0.3488889830379449 2207 18 -0.3014639176279914 2207 19 -0.88434672379028 2207 20 -0.8707569111843014 2207 21 -0.8061153665762504 2207 22 -0.08667996788055632 2207 23 -0.5300747282799847 2207 24 -1.5041248464222292 2207 25 -0.626639444987596 2208 1 0.8294454406586276 2208 2 -0.1796680743943142 2208 3 0.4427990858398374 2208 4 0.443249862749564 2208 5 0.5168304311897529 2208 6 0.18381000329522834 2208 7 0.06702284964575256 2208 8 0.2768738536569444 2208 9 -0.36906074154755686 2208 10 -0.14171727759570274 2208 11 -0.07517350350401841 2208 12 0.42715510586295835 2208 13 0.26340231774914713 2208 14 0.6444056434366416 2208 15 0.556964718819323 2208 16 0.3577281440154323 2208 17 0.7796672047287743 2208 18 0.6549135124336516 2208 19 -1.3685453961468497 2208 20 -0.10289299463617851 2208 21 -0.20277255007554373 2208 22 0.3597164285158385 2208 23 0.8775236595815556 2208 24 0.5754981976744986 2208 25 0.13155164083507032 2209 1 -0.2721019258204791 2209 2 -1.6604701221758837 2209 3 -0.8238276091145025 2209 4 -0.5077578856131311 2209 5 -0.8781400512380699 2209 6 -0.3661738542824864 2209 7 -0.8844230501789845 2209 8 -0.14852509477954356 2209 9 -2.3098526426636887 2209 10 -0.7967998345553937 2209 11 -0.6352348447087053 2209 12 -0.8491685727644764 2209 13 -0.19951623220011783 2209 14 -0.1618737588325203 2209 15 -0.4915663207526748 2209 16 0.10434976563705334 2209 17 -0.16135530242001295 2209 18 -0.10608639532393482 2209 19 -0.2636773027975252 2209 20 -0.6801575458768383 2209 21 -0.6060794405837981 2209 22 -0.5244501612821466 2209 23 -0.6221650222743591 2209 24 -0.575006829537017 2209 25 -0.2979405646810278 2210 1 0.10414059035377576 2210 2 -0.31561083533707546 2210 3 -0.40218225914150807 2210 4 -0.008326713827010224 2210 5 -0.3353285459692259 2210 6 -0.06406286957763996 2210 7 -0.3316599240370195 2210 8 -0.2398019130188196 2210 9 -0.16181940930622574 2210 10 -1.5525599384383675 2210 11 -0.9078357111662285 2210 12 -2.507791204083354 2210 13 -1.3969398425930137 2210 14 -0.9153700413390453 2210 15 -0.8243825375279386 2210 16 -0.38386572257843454 2210 17 -0.19258914252757364 2210 18 -0.26992041240027476 2210 19 -0.23692513952659056 2210 20 -0.45066121740440224 2210 21 -0.7255908228938139 2210 22 -0.25974394094452435 2210 23 -0.003050095992200852 2210 24 -0.31440306722677036 2210 25 0.03485266913610019 2211 1 0.15273651523836307 2211 2 0.09481646168816127 2211 3 -1.4582411296333682 2211 4 -0.14234733238371078 2211 5 0.2331743524305807 2211 6 0.3490719146911587 2211 7 0.4520534988516616 2211 8 0.5836213227755493 2211 9 0.6764602949528609 2211 10 0.9273595311718239 2211 11 1.256453226318584 2211 12 0.4456955535271163 2211 13 0.7397188404574209 2211 14 0.17109599358060024 2211 15 1.0700254599728467 2211 16 0.9256556075614526 2211 17 1.0298413479441564 2211 18 1.03422065462739 2211 19 1.138478380206827 2211 20 1.2147558397652318 2211 21 0.6645303198046477 2211 22 0.977924843116007 2211 23 0.659833030808457 2211 24 0.618465133682669 2211 25 0.5184862558624701 2212 1 -0.5321382653495377 2212 2 -0.9287687938187759 2212 3 -2.2109049779361705 2212 4 -1.7469358265368449 2212 5 -1.081580029630782 2212 6 -1.189828923401251 2212 7 -1.1115451067999018 2212 8 -1.4564043886141036 2212 9 -1.4262987349366263 2212 10 -0.9209803536213741 2212 11 -0.6528275964458814 2212 12 -0.7806759266083674 2212 13 -0.8042624693401024 2212 14 -1.008253310481308 2212 15 -1.0053286896971088 2212 16 -1.7769451983917222 2212 17 -1.6603240455142796 2212 18 -1.261769357486628 2212 19 -1.5291277410817088 2212 20 -1.1598393294602118 2212 21 -0.8107348289011327 2212 22 -1.2063442364656893 2212 23 -0.8075580048166557 2212 24 -0.08488970821700548 2212 25 -0.10602856527031435 2213 1 -2.1972062109930235 2213 2 -0.7620754818017232 2213 3 -1.7351788921381406 2213 4 -0.30462301012330784 2213 5 -0.49290430354498227 2213 6 0.46556676005198483 2213 7 0.06935017697308155 2213 8 0.4649118916949817 2213 9 0.8038371563503404 2213 10 0.4343087484303183 2213 11 0.731558228227712 2213 12 0.5666195703274184 2213 13 0.06029921464604371 2213 14 0.024360598391595545 2213 15 0.42275550961011277 2213 16 -1.232687271399982 2213 17 -0.4582457081841369 2213 18 0.5273859849061253 2213 19 -0.10325510585655451 2213 20 0.43957447283128914 2213 21 0.4868670895641003 2213 22 0.4836904024898132 2213 23 0.6656367726946684 2213 24 0.8451928923691909 2213 25 1.389734824018248 2214 1 0.14117762739081385 2214 2 0.03624101651477574 2214 3 -4.395448252407501 2214 4 -0.9855994410997404 2214 5 -0.6122645729052536 2214 6 0.14890009338532195 2214 7 0.38551179313469675 2214 8 0.2787166054996959 2214 9 0.5831300856432691 2214 10 0.1940730581946326 2214 11 -0.648576451906969 2214 12 -0.13068682697897316 2214 13 -3.1839768796300483 2214 14 -1.7604878864568896 2214 15 -0.7562007193461184 2214 16 -1.0547411434538532 2214 17 -1.139558839497269 2214 18 0.03984389736403671 2214 19 0.3135799109784614 2214 20 0.5493387825955978 2214 21 1.050503453200463 2214 22 0.8042291230285343 2214 23 0.6956031026610034 2214 24 0.5172467644230636 2214 25 0.7680176523010829 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55.0 3 9.0 4 11.0 5 13.0 6 4.0 7 5.0 8 6.0 9 30.0 10 57.0 11 203.0 12 476.0 13 866.0 14 1269.0 15 1792.0 16 2363.0 17 2612.0 18 2725.0 19 2624.0 20 2362.0 21 2220.0 22 2022.0 23 1839.0 24 1774.0 25 1786.0 26 1679.0 27 1755.0 28 2025.0 29 2243.0 30 2756.0 31 3404.0 32 4460.0 33 5606.0 34 7877.0 35 10505.0 36 14749.0 37 22336.0 38 38597.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.57412402640856 22.54730843790746 13.055266189730464 20.823301345953517 2 16.761702509084138 24.80952610785132 36.42619265960161 22.002578723462936 3 18.98940168111265 31.032319011467145 26.843328299649023 23.134951007771182 4 12.350641560429407 22.075056709667187 32.95986541365307 32.614436316250334 5 13.03277671295211 41.874077853311455 29.9961390493788 15.097006384357634 6 27.785478343022252 40.56726731075573 16.43047768789954 15.216776658322473 7 24.303022362586972 38.81629303746406 19.13128624525062 17.74939835469835 8 23.37061451897617 38.81081751985878 19.414028022947928 18.404539938217123 9 23.62859014584698 22.39216632762128 19.640037237527153 34.339206289004586 10 16.635175019998343 34.44542520618983 26.92604749951728 21.99335227429454 11 29.20924523878477 32.0708010977342 19.490298291340864 19.22965537214017 12 20.552441235890754 33.481579546159736 25.58729633383668 20.378682884112834 13 26.31687824521966 29.24886740541025 24.03376062777115 20.40049372159894 14 20.7338601790172 30.167432110003173 24.755540844332273 24.34316686664736 15 23.67101788066227 33.0409538192074 23.215209251329156 20.072819048801176 16 19.807202995386938 34.26422015818181 23.222523323886556 22.7060535225447 17 19.855740056269653 32.601919788161304 24.891046505213218 22.65129365035582 18 19.3139237186963 30.880333446046194 28.05377093526371 21.751971899993787 19 21.150013447626666 30.95505734205935 28.983428386215838 18.911500824098145 20 22.15334330890009 28.434502589280175 29.308169161707614 20.10398494011212 21 23.51558028726478 28.96368162291499 28.02314115691994 19.4975969329003 22 22.992365991076415 28.971994841699484 27.567254446275747 20.468384720948354 23 22.389788783384017 28.93384913492901 28.324955005275243 20.351407076411732 24 22.500086209869306 28.528569950687952 27.974757750267248 20.99658608917549 25 21.759189021446797 29.921384732087443 28.41941935038963 19.900006896076132 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1431.0 1 1431.0 2 1232.5 3 1034.0 4 1034.0 5 1034.0 6 1114.5 7 1195.0 8 1195.0 9 1195.0 10 1378.5 11 1562.0 12 1562.0 13 1562.0 14 1797.0 15 2032.0 16 2032.0 17 2032.0 18 2692.0 19 3352.0 20 3352.0 21 3352.0 22 4502.5 23 5653.0 24 5653.0 25 5653.0 26 7161.0 27 8669.0 28 8669.0 29 8669.0 30 9974.0 31 11279.0 32 11279.0 33 11279.0 34 12012.5 35 12746.0 36 12746.0 37 12746.0 38 12889.5 39 13033.0 40 13033.0 41 13033.0 42 13588.5 43 14144.0 44 14144.0 45 14144.0 46 16025.0 47 17906.0 48 17906.0 49 17906.0 50 17031.5 51 16157.0 52 16157.0 53 16157.0 54 14321.5 55 12486.0 56 12486.0 57 12486.0 58 10628.5 59 8771.0 60 8771.0 61 8771.0 62 7964.0 63 7157.0 64 7157.0 65 7157.0 66 5234.0 67 3311.0 68 3311.0 69 3311.0 70 2645.5 71 1980.0 72 1980.0 73 1980.0 74 1387.0 75 794.0 76 794.0 77 794.0 78 553.0 79 312.0 80 312.0 81 312.0 82 195.5 83 79.0 84 79.0 85 79.0 86 51.0 87 23.0 88 23.0 89 23.0 90 14.5 91 6.0 92 6.0 93 6.0 94 4.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11163559935223788 2 0.056506908314095716 3 0.06339799469386348 4 0.053750473762188604 5 0.05030493057230472 6 0.07166729834958481 7 0.0661544292457706 8 0.06270888605588672 9 0.0689108637976777 10 0.07097818971160803 11 0.06133066877993316 12 0.05926334286600282 13 0.06477621196981705 14 0.06959997243565448 15 0.06753264652172415 16 0.06339799469386348 17 0.06822175515970093 18 0.14057816214726251 19 0.07442373290149192 20 0.06477621196981705 21 0.06339799469386348 22 0.07304551562553838 23 0.06753264652172415 24 0.08269303655721325 25 0.07235640698756159 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 145115.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.30651552217208 #Duplication Level Percentage of deduplicated Percentage of total 1 91.27346831714132 62.34572580367295 2 5.835174480191277 7.971608724115358 3 1.2761922056434936 2.615167281121869 4 0.4479283314669653 1.2238569410467561 5 0.18764565237129627 0.6408710333184026 6 0.13115018714122856 0.5375047376218861 7 0.10189360693280067 0.4871998070495814 8 0.08272550265831341 0.45205526651276573 9 0.04943353207630923 0.30389690934775865 >10 0.4590256549943 6.749130000344554 >50 0.08978743581207188 4.365503221582882 >100 0.05750431282346176 6.747062674430623 >500 0.0040353903735762635 1.8254487820004823 >1k 0.0040353903735762635 3.7349688178341314 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1849 1.2741618716190608 No Hit GGTATCAACGCAGAGTACTTTTTTT 1256 0.865520449298832 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1241 0.8551838197291803 No Hit TATCAACGCAGAGTACTTTTTTTTT 1074 0.7401026771870586 No Hit GTACTGGTTCACTATCGGTCAGTCA 880 0.6064156014195637 No Hit ATACAGGGTGACAGCCCCGTACACA 648 0.4465423974089515 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 570 0.39279192364676296 No Hit GGATACCACGTGTCCCGCCCTACTC 551 0.37969885952520416 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 349 0.24049891465389517 No Hit GTTCACTATCGGTCAGTCAGGAGTA 324 0.22327119870447576 No Hit GTATTTAGCCTTGGAGGATGGTCCC 284 0.19570685318540468 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 266 0.1833028977018227 No Hit GGTATCAACGCAGAGTACATGGGGG 250 0.17227715949419425 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 249 0.1715880508562175 No Hit GCCTTGGAGGATGGTCCCCCCATAT 243 0.16745339902835682 No Hit CCCATATTCAGACAGGATACCACGT 236 0.16262963856251939 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 230 0.1584949867346587 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 226 0.15573855218275162 No Hit TATTCAGACAGGATACCACGTGTCC 225 0.15504944354477482 No Hit TCACGGTACTGGTTCACTATCGGTC 224 0.15436033490679807 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 223 0.15367122626882126 No Hit CATCTAAGTACCCCGAGGAAAAGAA 215 0.14815835716500705 No Hit CTATCGGTCAGTCAGGAGTATTTAG 211 0.14540192261309995 No Hit GGTTAATGAGGCGAACCGGGGGAAC 198 0.13644351031940186 No Hit CGGTACTGGTTCACTATCGGTCAGT 193 0.13299796712951797 No Hit ATCATTAACTGAATCCATAGGTTAA 192 0.1323088584915412 No Hit GTTAATGATAGTGTGTCGAAACACA 191 0.1316197498535644 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 191 0.1316197498535644 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 190 0.13093064121558765 No Hit CACACACACTGATTCAGGCTCTGGG 185 0.12748509802570376 No Hit ATCAACGCAGAGTACTTTTTTTTTT 180 0.12403955483581987 No Hit GGTATCAACGCAGAGTACATGGGGA 169 0.11645935981807531 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 168 0.11577025118009855 No Hit GCCCTACTCATCGAGCTCACAGCAT 166 0.114392033904145 No Hit GGTTCACTATCGGTCAGTCAGGAGT 165 0.11370292526616821 No Hit GTGGTATCAACGCAGAGTACTTTTT 165 0.11370292526616821 No Hit GTGGTATCAACGCAGAGTACATGGG 161 0.11094649071426112 No Hit GTGTGTGTTAGTGGAAGCGTCTGGA 159 0.10956827343830755 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 153 0.10543362161044689 No Hit GTACTTAGATGTTTCAGTTCCCCCG 148 0.10198807842056301 No Hit GGGTGACAGCCCCGTACACAAAAAT 147 0.10129896978258622 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAAAG 25 0.006002539 19.00207 2 GGTATCA 260 0.0 14.616976 1 CATCTAA 50 0.001486923 13.301449 1 GTACTGG 195 0.0 12.668047 1 GGGTGAC 120 7.166818E-10 12.663679 6 GGTGACA 130 1.9099389E-10 12.420147 7 ATCTAAG 55 0.0030429587 12.092226 2 TACTGGT 190 0.0 12.001307 2 ACTGGTT 190 0.0 12.001307 3 ACAGGGT 135 3.5652192E-10 11.964266 3 AGGGTGA 130 2.5320332E-9 11.689549 5 TGGTTCA 205 0.0 11.582633 5 CTGGTTC 210 0.0 11.310755 4 ATACAGG 135 4.5529305E-9 11.260486 1 GTGACAG 135 4.5765773E-9 11.256603 8 TACAGGG 145 1.1659722E-9 11.139144 2 GGTTCAC 215 0.0 11.043906 6 AGCCCCG 140 8.041752E-9 10.858326 13 TGACAGC 140 8.085408E-9 10.854583 9 CAGCCCC 150 2.044544E-9 10.767839 12 >>END_MODULE