FastQCFastQC Report
Wed 25 May 2016
SRR1294610_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294610_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145115
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48653.3525135237570205No Hit
GGTATCAACGCAGAGTACTTTTTTT36002.480791096716397No Hit
TATCAACGCAGAGTACTTTTTTTTT30142.076973434862006No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19141.3189539330875513No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9660.6656789442855666No Hit
GAGTACTTTTTTTTTTTTTTTTTTT8360.5760948213485856No Hit
GTACTGGTTCACTATCGGTCAGTCA8300.5719601695207249No Hit
ATACAGGGTGACAGCCCCGTACACA6950.47893050339386006No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6500.44792061468490507No Hit
GGATACCACGTGTCCCGCCCTACTC5540.3817661854391345No Hit
ATCAACGCAGAGTACTTTTTTTTTT4620.318368190745271No Hit
GTGGTATCAACGCAGAGTACTTTTT4220.2908038452261999No Hit
ACCCTGTATCGCGCGCCTTTCCAGA3970.2735761292767805No Hit
CCCATATTCAGACAGGATACCACGT3060.21086724322089379No Hit
GGTATCAACGCAGAGTACATGGGGG2980.20535437411707955No Hit
GTTCACTATCGGTCAGTCAGGAGTA2930.20190883092719566No Hit
GCCTTGGAGGATGGTCCCCCCATAT2810.19363952727147435No Hit
GCCCAGAGCCTGAATCAGTGTGTGT2770.19088309271956724No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG2590.17847913723598527No Hit
GTCAGGAGTATTTAGCCTTGGAGGA2560.17641181132205494No Hit
CGGTACTGGTTCACTATCGGTCAGT2360.16262963856251939No Hit
CTATCGGTCAGTCAGGAGTATTTAG2350.16194052992454258No Hit
GTATTTAGCCTTGGAGGATGGTCCC2260.15573855218275162No Hit
GGTTAATGAGGCGAACCGGGGGAAC2260.15573855218275162No Hit
CACACACACTGATTCAGGCTCTGGG2190.1509147917169142No Hit
CAACGCAGAGTACTTTTTTTTTTTT2140.14746924852703028No Hit
CATCTAAGTACCCCGAGGAAAAGAA2080.14333459669916962No Hit
TCTAAGTACCCCGAGGAAAAGAAAT2050.1412672707852393No Hit
TATTCAGACAGGATACCACGTGTCC2020.13919994487130896No Hit
GGGTGACAGCCCCGTACACAAAAAT1970.13575440168142508No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1960.1350652930434483No Hit
GTTAATGATAGTGTGTCGAAACACA1950.13437618440547153No Hit
AACGCAGAGTACTTTTTTTTTTTTT1910.1316197498535644No Hit
TCACGGTACTGGTTCACTATCGGTC1890.13024153257761087No Hit
GTGGTATCAACGCAGAGTACATGGG1850.12748509802570376No Hit
AGCTCACAGCATGTGCATTTTTGTG1810.12472866347379664No Hit
GGTTCACTATCGGTCAGTCAGGAGT1800.12403955483581987No Hit
ACTTAGATGTTTCAGTTCCCCCGGT1780.12266133755986632No Hit
TCAACGCAGAGTACTTTTTTTTTTT1770.12197222892188953No Hit
ATCATTAACTGAATCCATAGGTTAA1750.12059401164593599No Hit
GCCTGAATCAGTGTGTGTGTTAGTG1650.11370292526616821No Hit
GTCCCGCCCTACTCATCGAGCTCAC1650.11370292526616821No Hit
AGCGTACACGGTGGATGCCCTGGCA1600.11025738207628433No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG1600.11025738207628433No Hit
GGAGTATTTAGCCTTGGAGGATGGT1590.10956827343830755No Hit
GGTATCAACGCAGAGTACATGGGGA1560.10750094752437721No Hit
CCCCAGTAGCGGCGAGCGAACGGGG1530.10543362161044689No Hit
GCCCTACTCATCGAGCTCACAGCAT1470.10129896978258622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGTG1250.015.9756644
GGTATCA5500.014.7013031
GGTGACA1300.014.6297287
GGGTGAC1350.014.0878876
ACAGGGT1350.014.0830243
TACAGGG1550.013.4924452
GTACTGG1900.013.0172541
TACTGGT1900.013.0082662
AGGGTGA1501.8189894E-1212.6790975
GTGACAG1501.8189894E-1212.6790978
TGGTTCA2000.012.3621215
TGACAGC1551.8189894E-1212.2700949
ACAGCCC1650.012.08191511
CTGGTTC2050.012.0606054
ACTGGTT2100.011.7693833
GACAGCC1700.011.74681110
CCCCGTA1800.011.59847615
GGTTCAC2150.011.4996466
CCCGTAC1900.011.48748616
CCGTACA1900.011.48748617
Produced by FastQC (version 0.11.5)