FastQCFastQC Report
Wed 25 May 2016
SRR1294609_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294609_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986359
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT105211.066650175037689No Hit
GGTATCAACGCAGAGTACTTTTTTT70070.7103904359366113No Hit
TATCAACGCAGAGTACTTTTTTTTT62870.6373947011179499No Hit
CCCATGTACTCTGCGTTGATACCAC39000.39539356360108235No Hit
GTACATGGGAAGCAGTGGTATCAAC37050.37562388542102826No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA34940.35423207980055943No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28930.29330091781998235No Hit
GTACTGGTTCACTATCGGTCAGTCA26130.26491368761272516No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20100.2037797597020963No Hit
GTTAATGATAGTGTGTCGAAACACA19560.19830507959069668No Hit
GAGTACATGGGAAGCAGTGGTATCA17890.1813741244313683No Hit
ATACAGGGTGACAGCCCCGTACACA16340.1656597648523509No Hit
GGTATCAACGCAGAGTACATGGGGG16200.16424040334198806No Hit
CATGTACTCTGCGTTGATACCACTG15650.15866434026556253No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15210.15420348980442214No Hit
GGATACCACGTGTCCCGCCCTACTC14350.14548455481219313No Hit
ACTTAGATGTTTCAGTTCCCCCGGT14350.14548455481219313No Hit
GTGGTATCAACGCAGAGTACATGGG12240.1240927491917243No Hit
GTATCAACGCAGAGTACATGGGGGT11650.11811115425519511No Hit
GTACATGGGGGTTAAGCGACTAAGC11630.11790838832514328No Hit
AGCGTACACGGTGGATGCCCTGGCA11440.11598211198965082No Hit
GGTATCAACGCAGAGTACATGGGAA11350.11506966530441756No Hit
GCGTTGATACCACTGCTTCCCATGT11260.11415721861918429No Hit
ACGCAGAGTACATGGGAAGCAGTGG10910.11060881484327714No Hit
TCATTAACCTATGGATTCAGTTAAT10740.10888530443783652No Hit
GTATCAACGCAGAGTACATGGGAAG10700.10847977257773286No Hit
GTACTTAGATGTTTCAGTTCCCCCG10680.10827700664768102No Hit
TATCAACGCAGAGTACATGGGAAGC10630.10777009182255143No Hit
GCAGAGTACTTTTTTTTTTTTTTTT10580.10726317699742184No Hit
ACCCTGTATCGCGCGCCTTTCCAGA10560.10706041106736999No Hit
GTTCACTATCGGTCAGTCAGGAGTA10270.10412030508161836No Hit
ATCAACGCAGAGTACTTTTTTTTTT9910.1004705183406853No Hit
GTGGATGCCCTGGCAGTCAGAGGCG9880.10016636944560754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGAC400.00528936614.2450913
ATACCAT853.9543793E-612.2898846
TAAGACA701.0936307E-412.2100794
TAGATTG550.003075000412.0867445
GTATTAC752.0760516E-411.3978081
TAGAGCT752.0789889E-411.3960734
TAATACC855.3367396E-511.1726224
CTAGAAC600.00588815311.0795153
TTAGACA600.00588815311.0795154
TAGTAGA600.00588815311.0795154
TCCGAAT2150.011.05223917
TTCCGAA2150.011.05223916
CCGAATG2350.010.922770518
GTGTAGA700.001492956110.8550561
TTAGGAC700.001494853210.8534043
ACCATTC700.001494853210.8534048
TATACAG1158.8413435E-710.7354315
GTCATAT803.774738E-410.6854451
GTCCAAA803.774738E-410.6854451
CGCAGAA750.002652557810.1303572
Produced by FastQC (version 0.11.5)