##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294609_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 986359 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.01885824532447 31.0 28.0 33.0 18.0 34.0 2 28.96729791080124 31.0 28.0 34.0 16.0 34.0 3 28.97974368358782 31.0 28.0 34.0 16.0 34.0 4 32.2565860908655 35.0 32.0 37.0 19.0 37.0 5 32.99464697944663 35.0 32.0 37.0 26.0 37.0 6 32.92141299466016 35.0 33.0 37.0 23.0 37.0 7 32.857291310770215 35.0 33.0 37.0 23.0 37.0 8 32.61408067448059 35.0 32.0 37.0 19.0 37.0 9 33.41459549717699 37.0 32.0 39.0 17.0 39.0 10 33.67696041704897 37.0 32.0 39.0 17.0 39.0 11 33.88667919084228 38.0 33.0 39.0 19.0 39.0 12 33.7529915578405 37.0 32.0 39.0 18.0 39.0 13 33.477952753510635 37.0 32.0 39.0 17.0 39.0 14 34.21826637157465 38.0 32.0 40.0 17.0 41.0 15 34.224048242070076 38.0 32.0 40.0 17.0 41.0 16 34.394669689230795 38.0 32.0 40.0 17.0 41.0 17 34.4813369168832 38.0 32.0 40.0 18.0 41.0 18 34.408198232083855 38.0 32.0 40.0 18.0 41.0 19 34.73248685316401 39.0 32.0 40.0 20.0 41.0 20 34.85241479015247 39.0 33.0 41.0 21.0 41.0 21 34.92128829361318 39.0 33.0 41.0 22.0 41.0 22 35.00211282099114 39.0 33.0 41.0 23.0 41.0 23 35.02706823783227 39.0 33.0 41.0 24.0 41.0 24 35.089557655985296 39.0 33.0 41.0 24.0 41.0 25 33.9268521907338 38.0 31.0 40.0 20.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.156702900313288 1101 2 0.8880922168467897 1101 3 1.3631805213445745 1101 4 1.0505955696618443 1101 5 0.6052578599715659 1101 6 0.7435403188265752 1101 7 0.5740850483927247 1101 8 0.7651952293152746 1101 9 0.9185627392367834 1101 10 0.8563531154841826 1101 11 0.8546249294041104 1101 12 0.9014125232601415 1101 13 0.9220326238029415 1101 14 0.933266936113462 1101 15 1.1191422379759146 1101 16 1.027415930250605 1101 17 1.101644865951286 1101 18 0.9330409663838068 1101 19 0.8661977215390309 1101 20 0.849663103634974 1101 21 0.9036770486074346 1101 22 0.7079945946775652 1101 23 0.7810191938661859 1101 24 0.9033488071373412 1101 25 0.8883655280556795 1102 1 0.9196284726711461 1102 2 0.810455181843551 1102 3 1.012981815309825 1102 4 1.1058222951835148 1102 5 0.834057562880524 1102 6 0.8133734339176755 1102 7 0.7730577258729454 1102 8 0.9887659186791353 1102 9 1.2125271094319316 1102 10 0.9201590488569451 1102 11 0.7483172008129699 1102 12 1.1147425477595903 1102 13 0.8923147194916012 1102 14 1.3574682399348106 1102 15 1.269067613211753 1102 16 1.2868945073112315 1102 17 1.3841136784319232 1102 18 1.3026253909283128 1102 19 1.2596462304517573 1102 20 0.6122753927848095 1102 21 0.727095320410541 1102 22 0.7743481541380177 1102 23 0.7244794948996685 1102 24 0.8691586970306133 1102 25 0.9719417438761297 1103 1 0.8013834218418268 1103 2 0.8168757275899416 1103 3 1.0969839554917407 1103 4 0.9442385605660704 1103 5 0.7222330577975953 1103 6 0.5297993301188484 1103 7 0.559039534326665 1103 8 0.7538809534570916 1103 9 0.8925699130702398 1103 10 0.9255095036455998 1103 11 0.7927259755588238 1103 12 0.9654648591560573 1103 13 0.899270310716858 1103 14 0.797810669041283 1103 15 1.2754881589581402 1103 16 1.0134862676108582 1103 17 1.0218878892398422 1103 18 1.1271304738603618 1103 19 1.1788543631430386 1103 20 0.32281578871846506 1103 21 0.5291284932302318 1103 22 -0.22832707325630963 1103 23 0.4189685264673457 1103 24 0.6733320517657617 1103 25 0.7450824608370752 1104 1 0.40775515440197907 1104 2 0.3940556781493214 1104 3 0.7488926921022916 1104 4 0.4005765182004808 1104 5 0.34785462723938565 1104 6 0.2158747067733131 1104 7 0.21105591322289996 1104 8 0.31477238124566753 1104 9 0.5375296781682977 1104 10 0.5515161676816476 1104 11 0.2567632347559794 1104 12 0.48547783379306253 1104 13 0.45345337916094763 1104 14 0.5035750861280874 1104 15 0.7456722531698929 1104 16 0.5421943788600245 1104 17 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-0.16974789735719753 2207 11 0.19005516091070973 2207 12 0.3321662761991888 2207 13 0.5116894333525934 2207 14 0.6580612027077777 2207 15 0.5976464882924262 2207 16 0.6410813760816794 2207 17 0.6291634045658654 2207 18 0.8529880635080573 2207 19 -0.3475570261549521 2207 20 0.37509717851288826 2207 21 -0.03831178300768556 2207 22 0.37859845613533594 2207 23 -0.4334714463942646 2207 24 -1.3908499629838431 2207 25 -0.19226840213060825 2208 1 0.6660950225313442 2208 2 0.20274448523393218 2208 3 0.5776482936491902 2208 4 0.5154883299195561 2208 5 0.6204040513656182 2208 6 0.41341224240458985 2208 7 0.4843930698451331 2208 8 0.6415399090461378 2208 9 0.04012435245089563 2208 10 0.43021959118513564 2208 11 0.5310479140019169 2208 12 0.5402207276469966 2208 13 0.6890488737360201 2208 14 0.874537170448022 2208 15 0.7377462250544937 2208 16 0.6164754464113003 2208 17 0.8233658775341581 2208 18 1.0647236509691282 2208 19 -1.1699563717307697 2208 20 0.14673302921188736 2208 21 0.0853382049316167 2208 22 0.5751936675122451 2208 23 0.6061065339100864 2208 24 0.5728866522587879 2208 25 0.6313627081881066 2209 1 0.14742489761423272 2209 2 -1.370047121884653 2209 3 -0.18991495773774858 2209 4 0.10149806628802338 2209 5 0.03689584917534461 2209 6 0.019560899123469255 2209 7 -0.01274862232252616 2209 8 0.2804195882896394 2209 9 -2.0319686372814374 2209 10 -0.29082974551446483 2209 11 0.017369531293446983 2209 12 0.38968850166769897 2209 13 0.39242736064505124 2209 14 0.6423593787585347 2209 15 0.5400261785966975 2209 16 0.5735107345690906 2209 17 0.2632508038730137 2209 18 0.3108243671935256 2209 19 0.3191329442110913 2209 20 0.3334687716511695 2209 21 0.20980896493671963 2209 22 0.45474729373290046 2209 23 0.40741089427103816 2209 24 0.5781126397959042 2209 25 0.2771981866251778 2210 1 0.12016044104165502 2210 2 0.08456664691054883 2210 3 -0.01233456315646464 2210 4 0.07377872120704154 2210 5 0.11635731939106364 2210 6 -0.01919871299939757 2210 7 -0.08184149484936398 2210 8 0.17246398219648995 2210 9 0.24650793533312054 2210 10 -1.7111025682218575 2210 11 -0.4959948572340309 2210 12 -2.440693283522556 2210 13 -0.8551915627169038 2210 14 -0.4868881709153925 2210 15 -0.9198856738959833 2210 16 -0.13537441975679343 2210 17 -0.11526360502784883 2210 18 0.26452760626064276 2210 19 0.2512863106369565 2210 20 0.23247825380588694 2210 21 0.39707173543605734 2210 22 0.320057139798422 2210 23 0.2735457118840827 2210 24 0.21838264234509097 2210 25 0.28742201284723023 2211 1 -0.4423779015385172 2211 2 -0.09396928720626008 2211 3 -1.5326871646176592 2211 4 -0.8868991238345068 2211 5 -0.7622736550743241 2211 6 -0.5932229708928887 2211 7 -0.7896743804263906 2211 8 -0.2337672095761434 2211 9 -0.14258745380823967 2211 10 -0.20745842878658038 2211 11 -0.5483160466350725 2211 12 -0.4033679103761685 2211 13 -0.5884156025959655 2211 14 -0.9781194801309212 2211 15 -0.7707059178185673 2211 16 -0.3218997542101647 2211 17 -0.5098810834101499 2211 18 -0.15921343745564798 2211 19 -0.436045086193495 2211 20 -0.4905552785405405 2211 21 -0.5738499084311073 2211 22 -0.5059063781441964 2211 23 -0.7948171669671282 2211 24 -0.5524307014915948 2211 25 -0.596987404709246 2212 1 -0.3881588555641855 2212 2 -0.3565412684338938 2212 3 -2.1064527308880265 2212 4 -1.0928134809934882 2212 5 -0.6742279660440573 2212 6 -0.38345165965842654 2212 7 -0.23815918133675495 2212 8 -0.34059636510648517 2212 9 -0.8129955833763418 2212 10 -0.268151657340546 2212 11 -0.5083573636025847 2212 12 -0.1337462757032597 2212 13 -0.452586342582201 2212 14 -0.2069825651636421 2212 15 0.009497776130544366 2212 16 -1.0641061300808872 2212 17 -1.4288051965056 2212 18 -0.2001425170324893 2212 19 -0.10741657456254217 2212 20 -0.21719606074782405 2212 21 -0.10893143527496107 2212 22 -0.31140724080913174 2212 23 -0.13107215632118852 2212 24 -0.10189710519929207 2212 25 0.0075393259936689105 2213 1 -3.018298509964822 2213 2 -1.2933100931887473 2213 3 -2.0648759038824807 2213 4 -0.8004561452363355 2213 5 -0.6019313852475889 2213 6 -0.5954288383912285 2213 7 -0.42636800344909886 2213 8 0.047079854463355275 2213 9 -0.21841933612848408 2213 10 -0.03658914773011901 2213 11 -0.3064200161591586 2213 12 -0.27247124161829106 2213 13 -0.4238980187197612 2213 14 0.035219466152383916 2213 15 -0.323374826389184 2213 16 -1.63701720846435 2213 17 -0.7967445964507007 2213 18 -0.22624642008880613 2213 19 -0.19964816326945822 2213 20 -0.1345629357399929 2213 21 0.03574430477035406 2213 22 0.12129245951651058 2213 23 0.2241268121885156 2213 24 0.16867147876818223 2213 25 0.20386589472798988 2214 1 -0.9057212418030112 2214 2 -0.6580259283589918 2214 3 -5.875030473910861 2214 4 -2.1879395456421413 2214 5 -1.1055546469246025 2214 6 -0.35815407044849934 2214 7 -0.38015540730626896 2214 8 -0.3254790783091295 2214 9 -0.11194937043993036 2214 10 0.02487485221595165 2214 11 -0.6778801737626168 2214 12 -0.6106075032211606 2214 13 -4.795479513458449 2214 14 -3.1786372346909104 2214 15 -1.7160116845662898 2214 16 -2.621779286565019 2214 17 -3.0402783382857237 2214 18 -0.8009530201548642 2214 19 -0.717521797384066 2214 20 -0.3071144534653243 2214 21 -0.18863165160220774 2214 22 -0.24303914139216687 2214 23 -0.43875143947818174 2214 24 -0.15630307413817945 2214 25 -0.16328679457479467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 355.0 3 50.0 4 44.0 5 78.0 6 11.0 7 28.0 8 63.0 9 176.0 10 456.0 11 1114.0 12 2252.0 13 4292.0 14 7049.0 15 10335.0 16 13210.0 17 15226.0 18 15585.0 19 14893.0 20 14261.0 21 13265.0 22 12251.0 23 11404.0 24 11041.0 25 11245.0 26 11212.0 27 11830.0 28 13305.0 29 15904.0 30 19260.0 31 24094.0 32 31148.0 33 41167.0 34 55407.0 35 75700.0 36 105344.0 37 157002.0 38 266302.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36939245436583 20.89657810960319 13.12859565390794 21.605433782123036 2 16.08341600277526 23.899989247900805 36.95466062926153 23.061934120062404 3 19.512004617433654 29.409258603672644 27.663316863269387 23.415419915624312 4 12.753599667293871 20.792408619928896 33.159135978414675 33.294855734362564 5 12.945947234374033 41.12020707835526 30.923403525890375 15.010442161380332 6 28.856450699881712 38.747111301380286 16.985852224424747 15.410585774313256 7 24.990489793392666 37.66011383844112 19.440992591675858 17.908403776490363 8 24.85070788856778 37.344684977668315 19.136271465654445 18.66833566810946 9 24.898530994583922 20.776165612836273 19.37309856568831 34.95220482689149 10 16.907819520345402 32.909324971519176 27.981812851318438 22.201042656816984 11 30.54939882717283 29.8580982590181 20.00501104153585 19.587491872273215 12 21.42222420593057 31.798281206888156 26.835042929251944 19.94445165792933 13 27.24113340643757 26.98023041021214 24.022653521429653 21.75598266192064 14 21.239947087800168 27.849001403958678 25.474951916444173 25.436099591796985 15 24.598204639451875 31.503515550898136 22.957343702289496 20.940936107360493 16 20.94719885738863 32.075385570473884 24.96206173185151 22.01535384028598 17 20.59715028271971 31.406639601859233 25.674134849287167 22.322075266133893 18 21.03865411088236 28.926119036307007 28.07529409853773 21.959932754272906 19 21.632569832062185 30.04812715958455 28.705659404984367 19.613643603368903 20 23.244497284400467 27.266972750678136 28.48244953772928 21.00608042719211 21 23.499146890970437 28.080474251526162 27.980150252686638 20.440228604816767 22 23.34842161431757 27.749316766657806 26.939885610405838 21.962376008618783 23 22.656008277834978 27.942460931357882 28.131552652001236 21.269978138805904 24 22.4327823082183 28.090410864399935 27.495388960695077 21.981417866686687 25 22.403510106774203 27.810291917116842 28.023941768748887 21.762256207360064 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4547.0 1 4547.0 2 4442.0 3 4337.0 4 4337.0 5 4337.0 6 5617.5 7 6898.0 8 6898.0 9 6898.0 10 7716.0 11 8534.0 12 8534.0 13 8534.0 14 10184.0 15 11834.0 16 11834.0 17 11834.0 18 16242.5 19 20651.0 20 20651.0 21 20651.0 22 27251.5 23 33852.0 24 33852.0 25 33852.0 26 44362.0 27 54872.0 28 54872.0 29 54872.0 30 64128.5 31 73385.0 32 73385.0 33 73385.0 34 80443.5 35 87502.0 36 87502.0 37 87502.0 38 87138.0 39 86774.0 40 86774.0 41 86774.0 42 92046.5 43 97319.0 44 97319.0 45 97319.0 46 112240.0 47 127161.0 48 127161.0 49 127161.0 50 120352.0 51 113543.0 52 113543.0 53 113543.0 54 100408.0 55 87273.0 56 87273.0 57 87273.0 58 75048.5 59 62824.0 60 62824.0 61 62824.0 62 56012.0 63 49200.0 64 49200.0 65 49200.0 66 38480.5 67 27761.0 68 27761.0 69 27761.0 70 21753.5 71 15746.0 72 15746.0 73 15746.0 74 11590.5 75 7435.0 76 7435.0 77 7435.0 78 5247.5 79 3060.0 80 3060.0 81 3060.0 82 2111.0 83 1162.0 84 1162.0 85 1162.0 86 772.5 87 383.0 88 383.0 89 383.0 90 289.0 91 195.0 92 195.0 93 195.0 94 134.5 95 74.0 96 74.0 97 74.0 98 55.5 99 37.0 100 37.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09813871014508915 2 0.051198397338088876 3 0.05373297146373683 4 0.05109701437306295 5 0.045825100191715186 6 0.06295882128109542 7 0.05839658785492909 8 0.05474680111399602 9 0.05981594936529194 10 0.06164084273575848 11 0.05434126925389234 12 0.055862013729281124 13 0.05596339669430705 14 0.05829520488990317 15 0.060424247155447454 16 0.055456481869177454 17 0.058497970819955 18 0.13331859900908288 19 0.06458094872151011 20 0.058092438959851334 21 0.056064779659332965 22 0.06133669384068073 23 0.060221481225395626 24 0.06904179918265053 25 0.06427679982643236 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 986359.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.48599617977043 #Duplication Level Percentage of deduplicated Percentage of total 1 85.30023449168385 53.30070126580879 2 9.380915925225418 11.72351753332766 3 2.4991216311498023 4.684803140904246 4 0.9733280208751074 2.432774839762619 5 0.5039878804662201 1.5746092386731414 6 0.29923081694445686 1.1218641410676549 7 0.2004667006154345 0.8768453042179056 8 0.13876248349235856 0.6936569610719179 9 0.10115946858636209 0.5688945150871526 >10 0.506664100964682 5.634631773407364 >50 0.03946987326426984 1.7340500255489124 >100 0.0458508959133341 5.938406975187218 >500 0.00573136179774741 2.465775188288954 >1k 0.004585089438197928 4.812651265913464 >5k 3.2750638844270913E-4 1.3602794984278097 >10k+ 1.6375319422135456E-4 1.0765383333051741 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 10521 1.066650175037689 No Hit GGTATCAACGCAGAGTACTTTTTTT 7007 0.7103904359366113 No Hit TATCAACGCAGAGTACTTTTTTTTT 6287 0.6373947011179499 No Hit CCCATGTACTCTGCGTTGATACCAC 3900 0.39539356360108235 No Hit GTACATGGGAAGCAGTGGTATCAAC 3705 0.37562388542102826 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 3494 0.35423207980055943 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2893 0.29330091781998235 No Hit GTACTGGTTCACTATCGGTCAGTCA 2613 0.26491368761272516 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2010 0.2037797597020963 No Hit GTTAATGATAGTGTGTCGAAACACA 1956 0.19830507959069668 No Hit GAGTACATGGGAAGCAGTGGTATCA 1789 0.1813741244313683 No Hit ATACAGGGTGACAGCCCCGTACACA 1634 0.1656597648523509 No Hit GGTATCAACGCAGAGTACATGGGGG 1620 0.16424040334198806 No Hit CATGTACTCTGCGTTGATACCACTG 1565 0.15866434026556253 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1521 0.15420348980442214 No Hit GGATACCACGTGTCCCGCCCTACTC 1435 0.14548455481219313 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 1435 0.14548455481219313 No Hit GTGGTATCAACGCAGAGTACATGGG 1224 0.1240927491917243 No Hit GTATCAACGCAGAGTACATGGGGGT 1165 0.11811115425519511 No Hit GTACATGGGGGTTAAGCGACTAAGC 1163 0.11790838832514328 No Hit AGCGTACACGGTGGATGCCCTGGCA 1144 0.11598211198965082 No Hit GGTATCAACGCAGAGTACATGGGAA 1135 0.11506966530441756 No Hit GCGTTGATACCACTGCTTCCCATGT 1126 0.11415721861918429 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1091 0.11060881484327714 No Hit TCATTAACCTATGGATTCAGTTAAT 1074 0.10888530443783652 No Hit GTATCAACGCAGAGTACATGGGAAG 1070 0.10847977257773286 No Hit GTACTTAGATGTTTCAGTTCCCCCG 1068 0.10827700664768102 No Hit TATCAACGCAGAGTACATGGGAAGC 1063 0.10777009182255143 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1058 0.10726317699742184 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 1056 0.10706041106736999 No Hit GTTCACTATCGGTCAGTCAGGAGTA 1027 0.10412030508161836 No Hit ATCAACGCAGAGTACTTTTTTTTTT 991 0.1004705183406853 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 988 0.10016636944560754 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.0138296502591855E-4 0.0 2 0.0 0.0 0.0 1.0138296502591855E-4 0.0 3 0.0 0.0 0.0 1.0138296502591855E-4 0.0 4 0.0 0.0 0.0 1.0138296502591855E-4 0.0 5 0.0 0.0 0.0 1.0138296502591855E-4 0.0 6 0.0 0.0 0.0 1.0138296502591855E-4 0.0 7 0.0 0.0 0.0 1.0138296502591855E-4 0.0 8 0.0 0.0 0.0 1.0138296502591855E-4 0.0 9 0.0 0.0 0.0 1.0138296502591855E-4 0.0 10 0.0 0.0 0.0 1.0138296502591855E-4 0.0 11 0.0 0.0 0.0 1.0138296502591855E-4 0.0 12 0.0 0.0 0.0 1.0138296502591855E-4 0.0 13 0.0 0.0 0.0 1.0138296502591855E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGAC 40 0.005289366 14.245091 3 ATACCAT 85 3.9543793E-6 12.289884 6 TAAGACA 70 1.0936307E-4 12.210079 4 TAGATTG 55 0.0030750004 12.086744 5 GTATTAC 75 2.0760516E-4 11.397808 1 TAGAGCT 75 2.0789889E-4 11.396073 4 TAATACC 85 5.3367396E-5 11.172622 4 CTAGAAC 60 0.005888153 11.079515 3 TTAGACA 60 0.005888153 11.079515 4 TAGTAGA 60 0.005888153 11.079515 4 TCCGAAT 215 0.0 11.052239 17 TTCCGAA 215 0.0 11.052239 16 CCGAATG 235 0.0 10.9227705 18 GTGTAGA 70 0.0014929561 10.855056 1 TTAGGAC 70 0.0014948532 10.853404 3 ACCATTC 70 0.0014948532 10.853404 8 TATACAG 115 8.8413435E-7 10.735431 5 GTCATAT 80 3.774738E-4 10.685445 1 GTCCAAA 80 3.774738E-4 10.685445 1 CGCAGAA 75 0.0026525578 10.130357 2 >>END_MODULE