FastQCFastQC Report
Wed 25 May 2016
SRR1294609_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294609_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986359
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT254192.5770535879938237No Hit
GGTATCAACGCAGAGTACTTTTTTT174991.774100504988549No Hit
TATCAACGCAGAGTACTTTTTTTTT159751.619592866289049No Hit
ACGCAGAGTACTTTTTTTTTTTTTT95850.9717557197734294No Hit
GTACTTTTTTTTTTTTTTTTTTTTT54590.5534496060764894No Hit
GAGTACTTTTTTTTTTTTTTTTTTT44550.45166110919046715No Hit
CCCATGTACTCTGCGTTGATACCAC43180.4377716429819163No Hit
GTACATGGGAAGCAGTGGTATCAAC37540.3805916507072982No Hit
GCAGAGTACTTTTTTTTTTTTTTTT33500.33963293283682716No Hit
ATCAACGCAGAGTACTTTTTTTTTT28220.2861027273031422No Hit
GTACTGGTTCACTATCGGTCAGTCA24700.2504159236140188No Hit
GTGGTATCAACGCAGAGTACTTTTT20200.2047935893523555No Hit
GAGTACATGGGAAGCAGTGGTATCA19600.19871061145080035No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA18680.18938337866841584No Hit
GTTAATGATAGTGTGTCGAAACACA18630.18887646384328627No Hit
ATACAGGGTGACAGCCCCGTACACA17830.18076582664121277No Hit
CATGTACTCTGCGTTGATACCACTG17140.1737704020544244No Hit
GGTATCAACGCAGAGTACATGGGGG17110.17346625315934663No Hit
ACTTAGATGTTTCAGTTCCCCCGGT15740.1595767869507958No Hit
GGATACCACGTGTCCCGCCCTACTC15650.15866434026556253No Hit
AGCGTACACGGTGGATGCCCTGGCA13450.13636008795986046No Hit
ACGCAGAGTACATGGGAAGCAGTGG13220.13402827976426432No Hit
GTGGTATCAACGCAGAGTACATGGG12720.1289591315129684No Hit
GGTATCAACGCAGAGTACATGGGAA12680.12855359965286473No Hit
GCGTTGATACCACTGCTTCCCATGT12620.12794530186270922No Hit
CAACGCAGAGTACTTTTTTTTTTTT12510.12683008924742412No Hit
ACCCTGTATCGCGCGCCTTTCCAGA11930.12094987727592084No Hit
GTATCAACGCAGAGTACATGGGGGT11900.12064572838084307No Hit
GTATCAACGCAGAGTACATGGGAAG11610.11770562239509144No Hit
TATCAACGCAGAGTACATGGGAAGC11590.11750285646503962No Hit
ACTCTGCGTTGATACCACTGCTTCC10890.11040604891322532No Hit
GTGGATGCCCTGGCAGTCAGAGGCG10660.10807424071762918No Hit
AACGCAGAGTACTTTTTTTTTTTTT10530.10675626217229225No Hit
TCATTAACCTATGGATTCAGTTAAT10060.10199126281607407No Hit
GTACTTAGATGTTTCAGTTCCCCCG10050.10188987985104815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC402.7584765E-416.6260683
GTCTTAG400.005243119314.2639561
AGGACCG500.001474220713.32928855
GTCCTAA853.8962826E-612.3061581
CCGATAG701.10978E-412.19091917
GTACTAA550.00304466512.1027521
CTGTTAG802.8353092E-511.886631
GCGTACT802.9190247E-511.85288312
GCACCCG657.872184E-411.7180586
GGACCGT657.872184E-411.7180586
GGTATCA43500.011.6079091
ATAGGGT752.0711089E-411.4007313
CGATAGT752.1095325E-411.37819118
ATTGGAC600.00587026411.0840443
ATCGCCG1553.2196112E-1011.01171119
AATCGCC1553.2196112E-1011.01115218
TAGACAG700.001463450510.8810535
GTGTAAA700.001478436310.8677761
CTAGAGC700.001489763110.857843
ATTATAC700.001489763110.857843
Produced by FastQC (version 0.11.5)