FastQCFastQC Report
Wed 25 May 2016
SRR1294608_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294608_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762288
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT175522.3025418214638034No Hit
GGTATCAACGCAGAGTACTTTTTTT114181.4978590768843272No Hit
TATCAACGCAGAGTACTTTTTTTTT101771.3350597149633734No Hit
GTACTGGTTCACTATCGGTCAGTCA46840.6144659236404089No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43030.5644848141384883No Hit
ATACAGGGTGACAGCCCCGTACACA28890.3789906177192872No Hit
GTTAATGATAGTGTGTCGAAACACA27030.35459039103331025No Hit
GGATACCACGTGTCCCGCCCTACTC26620.3492118464412401No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26560.34842474235459564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA25510.3346504208383183No Hit
CCCATGTACTCTGCGTTGATACCAC22590.29634468862162333No Hit
GTACATGGGAAGCAGTGGTATCAAC22560.29595113657830113No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21950.2879489116974162No Hit
ACTTAGATGTTTCAGTTCCCCCGGT19640.2576454043616061No Hit
GGTATCAACGCAGAGTACATGGGGG18720.24557647503305838No Hit
ACCCTGTATCGCGCGCCTTTCCAGA18560.24347753080200657No Hit
GTTCACTATCGGTCAGTCAGGAGTA17500.22957202527128856No Hit
GCAGAGTACTTTTTTTTTTTTTTTT17310.22707952899691455No Hit
AGCGTACACGGTGGATGCCCTGGCA15340.2012362781520895No Hit
GTATTTAGCCTTGGAGGATGGTCCC15280.20044917406544507No Hit
ATCAACGCAGAGTACTTTTTTTTTT15010.1969072056755452No Hit
TCATTAACCTATGGATTCAGTTAAT14850.19480826144449342No Hit
GTGGTATCAACGCAGAGTACATGGG14740.19336523728564534No Hit
GTACTTAGATGTTTCAGTTCCCCCG14640.19205339714123798No Hit
GTATCAACGCAGAGTACATGGGGGT14150.18562538043364188No Hit
GTACATGGGGGTTAAGCGACTAAGC13980.18339525218814937No Hit
CTATCGGTCAGTCAGGAGTATTTAG13620.17867262766828285No Hit
GCCTTGGAGGATGGTCCCCCCATAT13570.17801670759607918No Hit
GTGGATGCCCTGGCAGTCAGAGGCG13360.1752618432928237No Hit
CCCATATTCAGACAGGATACCACGT13020.17080158680183868No Hit
GTCAGGAGTATTTAGCCTTGGAGGA12280.16109396973322418No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG12200.1600444976176983No Hit
ATCATTAACTGAATCCATAGGTTAA12160.15951976155993536No Hit
GTGGTATCAACGCAGAGTACTTTTT12030.15781436937220578No Hit
GCCCAGAGCCTGAATCAGTGTGTGT11630.15256700879457633No Hit
GGGTACTTAGATGTTTCAGTTCCCC11480.15059924857796528No Hit
TATTCAGACAGGATACCACGTGTCC11440.15007451252020235No Hit
GGTTAATGAGGCGAACCGGGGGAAC11130.14600780807253952No Hit
GGGTTTCCCCATTCGGAAATCGCCG11040.1448271519425729No Hit
GAGTACATGGGAAGCAGTGGTATCA10800.14167873559599523No Hit
CCTATGGATTCAGTTAATGATAGTG10740.1408916315093508No Hit
GGTATCAACGCAGAGTACATGGGAA10660.1398421593938249No Hit
CGGTACTGGTTCACTATCGGTCAGT10540.13826795122053606No Hit
GTGCTAATCTGCGATAAGCGTCGGT10480.13748084713389166No Hit
TCACGGTACTGGTTCACTATCGGTC10310.13525071888839912No Hit
ACGCAGAGTACATGGGGGTTAAGCG10240.134332430787314No Hit
TCTAAGTACCCCGAGGAAAAGAAAT10220.1340700627584325No Hit
GGTATCAACGCAGAGTACATGGGGA10150.13315177465734734No Hit
ATGATAGTGTGTCGAAACACACTGG9630.12633020590642904No Hit
CACACACACTGATTCAGGCTCTGGG9580.12567428583422538No Hit
GGTTCACTATCGGTCAGTCAGGAGT9340.12252586948764772No Hit
CATGTACTCTGCGTTGATACCACTG9160.12016455722771446No Hit
CATCTAAGTACCCCGAGGAAAAGAA8560.11229351636127029No Hit
GTCCCGCCCTACTCATCGAGCTCAC8490.11137522826018512No Hit
TAATGATAGTGTGTCGAAACACACT8250.10822681191360746No Hit
AGCTCACAGCATGTGCATTTTTGTG8140.10678378775475934No Hit
GTATCAACGCAGAGTACATGGGAAG7900.10363537140818167No Hit
GTTATAACCGGCGATTTCCGAATGG7760.10179879520601139No Hit
TATCAACGCAGAGTACATGGGGGTT7630.1000934030182818No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA401.2880961E-518.9926471
GTACAAG2600.015.3402141
CAAGGAC508.7432214E-515.19212154
TCTGAAC400.00529400814.2426153
GTGGGTC400.00529400814.24261511
GTACAAA2150.014.1340631
GTATCAC551.9645267E-413.8128341
GTGTTAC500.001502700313.2948521
ATCGCCG1800.013.19107119
AATCGCC1850.012.85143318
CCGAATG1950.012.68008118
CGAATGG1950.012.66342719
TCCAAGA751.4841295E-512.6601022
GGTATCA32000.012.6419791
CATCGCC951.0275271E-612.00954816
AAATCGC2000.011.88444917
GAAATCG2050.011.59458416
TACAAGA2300.011.5592242
TACATGA2150.011.4824182
GTATAAA1001.9341242E-611.3955871
Produced by FastQC (version 0.11.5)