##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294608_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	762288
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.210824517767563	31.0	27.0	33.0	16.0	34.0
2	28.094912946288016	31.0	27.0	33.0	16.0	34.0
3	28.214754790840207	31.0	27.0	34.0	16.0	34.0
4	30.995232772915223	35.0	30.0	37.0	12.0	37.0
5	32.30247229393615	35.0	32.0	37.0	20.0	37.0
6	31.978576338601684	35.0	32.0	37.0	17.0	37.0
7	31.94211636546817	35.0	32.0	37.0	17.0	37.0
8	31.607054026824507	35.0	31.0	37.0	16.0	37.0
9	31.960458514367275	37.0	30.0	39.0	10.0	39.0
10	32.46633162269379	37.0	31.0	39.0	13.0	39.0
11	32.6364707302227	37.0	31.0	39.0	13.0	39.0
12	32.614304567302646	37.0	31.0	39.0	15.0	39.0
13	32.182232961820205	37.0	30.0	39.0	12.0	39.0
14	32.84001191150851	38.0	30.0	40.0	12.0	41.0
15	32.882683447725796	38.0	30.0	40.0	13.0	41.0
16	33.144167296349934	38.0	30.0	40.0	13.0	41.0
17	33.37812873874441	38.0	31.0	40.0	16.0	41.0
18	33.36111023655101	38.0	31.0	40.0	16.0	41.0
19	33.77929207858447	38.0	31.0	40.0	17.0	41.0
20	33.91050626534853	38.0	31.0	40.0	18.0	41.0
21	34.064076831853576	38.0	31.0	41.0	18.0	41.0
22	34.218673257351554	38.0	31.0	41.0	19.0	41.0
23	34.345275014167875	38.0	31.0	40.0	21.0	41.0
24	34.48662316604748	38.0	31.0	41.0	23.0	41.0
25	33.55706898180215	37.0	30.0	40.0	20.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.446011775244802
1101	2	1.302407571661643
1101	3	1.640477591110674
1101	4	1.6542772374391816
1101	5	1.224717859140938
1101	6	1.3194152863627373
1101	7	1.023323632695849
1101	8	1.3114278675201163
1101	9	1.3925121670330576
1101	10	1.4612552045562452
1101	11	1.3421500838239737
1101	12	1.4595072979179733
1101	13	1.385039993494786
1101	14	1.4343692372926142
1101	15	1.598613204103657
1101	16	1.578191848058168
1101	17	1.5594088756603455
1101	18	1.6834435782171653
1101	19	1.4979059719722727
1101	20	1.1626648448401014
1101	21	1.131299149317023
1101	22	1.0626588447930914
1101	23	1.2699479937870066
1101	24	1.0765939217401552
1101	25	1.2138431231122766
1102	1	1.1855309956164781
1102	2	1.013362709047744
1102	3	1.2063094881665428
1102	4	1.3391438015618888
1102	5	0.9292505742680888
1102	6	0.670201137944705
1102	7	0.7239668128238037
1102	8	0.7287060095471745
1102	9	0.9917107573110933
1102	10	0.9413137517128618
1102	11	0.8305730622036762
1102	12	0.9069069920515602
1102	13	1.0718535319454574
1102	14	1.0965087794640027
1102	15	0.9912432800847597
1102	16	1.1900151517243742
1102	17	0.9408199879239092
1102	18	0.9676775549880858
1102	19	0.9511405203117462
1102	20	0.1178689874983121
1102	21	0.43685384666154903
1102	22	0.5491116771469606
1102	23	0.47588756199896665
1102	24	0.6511963064388908
1102	25	0.8715143895448705
1103	1	0.8734912560741286
1103	2	0.9413047445759055
1103	3	1.2707456909857662
1103	4	1.000837277894039
1103	5	0.9259321531907005
1103	6	0.49629831736516294
1103	7	0.3744499720066514
1103	8	0.6342347258287049
1103	9	0.817029928703807
1103	10	0.8142962342533977
1103	11	0.5670138296153269
1103	12	0.7936715550137805
1103	13	0.6148179672077703
1103	14	0.6832757459432983
1103	15	1.0010417922031891
1103	16	0.8652268441034465
1103	17	0.9293060961177062
1103	18	0.9470679185566198
1103	19	0.6667563797624254
1103	20	0.2598180935467127
1103	21	0.29080517648574755
1103	22	0.04322776284175234
1103	23	0.20280308673376624
1103	24	0.6058685868854425
1103	25	0.6055939626802385
1104	1	0.9502824632620559
1104	2	1.0915669564771662
1104	3	1.189154020165045
1104	4	1.0623547431519746
1104	5	1.0143222855935292
1104	6	0.6069014857927435
1104	7	0.4986601954338852
1104	8	0.5849615837033681
1104	9	1.138414788499471
1104	10	1.0789387628353069
1104	11	0.9095610541003296
1104	12	1.0678663780511428
1104	13	0.9087384606686868
1104	14	1.1520159940516876
1104	15	1.3718730485102597
1104	16	0.888698254090194
1104	17	1.0296852293999734
1104	18	0.9463840196642366
1104	19	0.4932801064946659
1104	20	0.6820755070107083
1104	21	0.38052460569866753
1104	22	0.6677571390854311
1104	23	0.22888648389766786
1104	24	-0.171745730613857
1104	25	-1.3079703359738488
1105	1	-0.1539198133861639
1105	2	-0.24203432329078822
1105	3	0.4241022039065747
1105	4	-0.015201306460948416
1105	5	0.054793154138408795
1105	6	0.10882193458807521
1105	7	0.06559102341824996
1105	8	0.2049717039107115
1105	9	0.3201990129290131
1105	10	-1.535000229048606
1105	11	-0.29443682623419676
1105	12	-0.1059710930126343
1105	13	0.01909653272934264
1105	14	0.44521413894596407
1105	15	-0.48877488381982204
1105	16	-0.3303026344161921
1105	17	-0.5064158212687957
1105	18	-0.8748718024432378
1105	19	-1.8474471539173933
1105	20	-0.7704481698917149
1105	21	-1.5315570890774737
1105	22	-0.8058755372312874
1105	23	-0.2952924445556562
1105	24	-0.9726824474432831
1105	25	-0.6379697781671467
1106	1	-0.36185862858680196
1106	2	-0.2977311987744713
1106	3	0.29113024581802804
1106	4	0.2439323350844198
1106	5	-0.006637272715067866
1106	6	-0.4614780001355747
1106	7	-0.07037893600274359
1106	8	-0.04242228931938996
1106	9	0.20057169376805106
1106	10	0.0018539893512468097
1106	11	-0.47453561559347435
1106	12	-0.07175821136470972
1106	13	-0.11588755195494116
1106	14	-0.5103912150604586
1106	15	-0.15995032914583618
1106	16	0.08233809667889602
1106	17	-0.006436009829954514
1106	18	-0.15517315036617418
1106	19	0.060842942247454346
1106	20	-0.20251702634431723
1106	21	-0.11797086036799698
1106	22	-0.16127833267405833
1106	23	-0.5637431070769381
1106	24	-0.41391018114270395
1106	25	-0.3010361514267217
1107	1	0.6678110721702453
1107	2	0.8116443929993977
1107	3	1.0063055371285792
1107	4	0.8370507151073951
1107	5	0.5990950961057635
1107	6	0.5958204390542683
1107	7	0.6162239348106553
1107	8	0.5922212576627217
1107	9	0.8295563044913088
1107	10	-0.8446898408406867
1107	11	0.1886614151219561
1107	12	0.8591278608558994
1107	13	0.6054821652139708
1107	14	0.861546815431943
1107	15	1.0241486806718285
1107	16	0.8702547580853519
1107	17	0.365640890263883
1107	18	0.22647102363323057
1107	19	0.5406763408074156
1107	20	0.678723163359976
1107	21	0.5761751547056733
1107	22	0.7347367409899519
1107	23	0.6873447048010419
1107	24	0.6887470676400795
1107	25	0.583523232160573
1108	1	-0.003454277425330332
1108	2	0.09304557635768873
1108	3	0.4689037036494277
1108	4	0.020516205101415608
1108	5	0.23192723697450646
1108	6	0.2484034591423132
1108	7	0.2420430698041045
1108	8	0.3894570336753347
1108	9	0.6693896937304196
1108	10	0.7753724393544914
1108	11	0.3355316710551719
1108	12	0.2869722260716685
1108	13	0.4711189551384791
1108	14	0.8116336134829609
1108	15	0.5114592205833688
1108	16	0.5765228914431262
1108	17	0.4497200182719894
1108	18	0.47442210549658625
1108	19	0.30149670342269985
1108	20	0.3219373539768
1108	21	0.4700567566311378
1108	22	0.4174526900453728
1108	23	0.40492857212105804
1108	24	0.5619207640267234
1108	25	0.2433042659219069
1109	1	-0.23779409662753537
1109	2	-0.2362786162692565
1109	3	0.3058370302616531
1109	4	-0.025730896420917304
1109	5	0.049407997803889714
1109	6	0.20242520192552504
1109	7	0.06686496045168155
1109	8	0.18501606180317154
1109	9	0.3785161177651055
1109	10	0.2825833477121833
1109	11	0.0010624705309254523
1109	12	0.007793377397405266
1109	13	0.2631947692326406
1109	14	0.34286912109461554
1109	15	-0.48533985190377393
1109	16	-0.013484501775280933
1109	17	0.16662864481364892
1109	18	0.0714984236738232
1109	19	0.36942777846386576
1109	20	0.31461890789688596
1109	21	0.18250760853153025
1109	22	0.16719056684747358
1109	23	0.1893608065532888
1109	24	0.29530954649807484
1109	25	0.3180320610625458
1110	1	-0.25782721486304894
1110	2	-0.15039654450169948
1110	3	-0.1624659162370321
1110	4	-0.22297303510779543
1110	5	-0.2613460395480196
1110	6	-0.21228765955831008
1110	7	-0.20268418769267527
1110	8	-0.1088536503754618
1110	9	-0.04435851093509058
1110	10	-4.1624257768546613E-4
1110	11	-0.40186695689916974
1110	12	-0.1050843241287609
1110	13	-0.21884968431647067
1110	14	-0.22391619079721892
1110	15	0.025952783556633108
1110	16	-0.45602436315668626
1110	17	-0.09283224494018327
1110	18	-4.824875641674943
1110	19	-2.2188553653785306
1110	20	-0.9633292979141714
1110	21	-0.5414604340783242
1110	22	-0.389200911181824
1110	23	-0.616947022324446
1110	24	-0.010219833132502743
1110	25	-0.2629189785769128
1111	1	-0.9815376160735916
1111	2	-0.8971176173412942
1111	3	-1.9764712771494644
1111	4	-1.3143677393586515
1111	5	-1.0799319962208216
1111	6	-0.6670977414322401
1111	7	-0.8652702392811875
1111	8	-2.136302544196724
1111	9	-0.7192708279827755
1111	10	-0.4828157665388808
1111	11	-0.8895475363230858
1111	12	-0.5929460647916578
1111	13	-1.033916002107052
1111	14	-0.5578123256286389
1111	15	-0.5572937718700359
1111	16	-2.238286350600429
1111	17	-1.877564778852019
1111	18	-0.92015550156475
1111	19	-0.5717072630995474
1111	20	-0.6721374759979497
1111	21	-0.5061832756357134
1111	22	-0.5823981925767896
1111	23	-0.8494530982989943
1111	24	-0.7473320590976087
1111	25	-0.837523640946209
1112	1	-0.3948054808173431
1112	2	-0.50849906525319
1112	3	0.0018286251121715225
1112	4	-0.5023534094542192
1112	5	-1.6117713829813027
1112	6	-0.7485030883181203
1112	7	-0.8187691495600227
1112	8	-0.1775384652741785
1112	9	-0.5494895211326529
1112	10	-0.26827578623823456
1112	11	-0.5650509457899631
1112	12	-1.5407570003023494
1112	13	-1.3293257116695791
1112	14	-2.482040443752709
1112	15	-3.3461495125059706
1112	16	-1.2371838314912047
1112	17	-0.6482825159481393
1112	18	-0.2407581993711787
1112	19	-0.1163659229937366
1112	20	-0.22432938447464323
1112	21	-0.09978838040346716
1112	22	-0.05971123121614852
1112	23	-0.1295507797856814
1112	24	0.03159532158053224
1112	25	0.26486417086145764
1113	1	-0.6271905045564701
1113	2	-0.06317647887059863
1113	3	-0.4960705301557411
1113	4	-2.326024740247288
1113	5	-0.589267862392397
1113	6	-0.1847610737252019
1113	7	-0.4874069275191495
1113	8	-0.8158625603311229
1113	9	-1.7984040498131577
1113	10	-0.7499634927393437
1113	11	-0.32208427682157037
1113	12	-1.8907024913329558
1113	13	-1.2450910448020345
1113	14	-0.39060870969789363
1113	15	-0.12542480477239337
1113	16	-0.22569405218452943
1113	17	-0.0361080788761754
1113	18	0.2992070236971003
1113	19	0.11932207226890057
1113	20	0.3964461703973683
1113	21	0.2517965646586049
1113	22	0.03352944733158836
1113	23	0.1399620711485028
1113	24	0.26122560220233026
1113	25	-0.40075939710107633
1201	1	1.3812732086888424
1201	2	1.2907518526440889
1201	3	1.6852942730435814
1201	4	1.503788120514706
1201	5	1.0783976577609309
1201	6	1.0347706239855476
1201	7	0.8323099244326002
1201	8	1.0879099433108408
1201	9	1.0733057054939046
1201	10	1.2039453252453995
1201	11	1.149765213303347
1201	12	1.5556650202097444
1201	13	1.3212263595844718
1201	14	1.5946810045410444
1201	15	1.615461311351055
1201	16	1.7888771716390295
1201	17	1.4114605240784357
1201	18	1.667512565203161
1201	19	1.2670675306069583
1201	20	1.0057067982620183
1201	21	0.921721841296538
1201	22	1.0314233318585906
1201	23	1.0605464901555806
1201	24	1.187388199251302
1201	25	1.021335077541984
1202	1	0.949984910935644
1202	2	1.0196520238340447
1202	3	1.0570591295053369
1202	4	0.8245319702088558
1202	5	0.49517907325122223
1202	6	0.4648813908205618
1202	7	0.46730307150130557
1202	8	0.7137100297057728
1202	9	0.9053976894308455
1202	10	0.39983569967677113
1202	11	0.4219257798834448
1202	12	0.17335784889712613
1202	13	0.5700016314351899
1202	14	0.7915547879457492
1202	15	0.8452910280076793
1202	16	0.6474913329415628
1202	17	0.45654747897708603
1202	18	0.7429428066289958
1202	19	0.5425831003601971
1202	20	0.5166618888966994
1202	21	0.3767522128480749
1202	22	0.3603560344733694
1202	23	0.3467579657490134
1202	24	0.4652689635797955
1202	25	0.5438068258660849
1203	1	0.6032223210075891
1203	2	0.540515333186562
1203	3	0.7325986978506585
1203	4	0.5720139845973442
1203	5	0.3779128862008605
1203	6	0.2548094483745196
1203	7	0.21046853912719854
1203	8	0.22162369877052157
1203	9	0.47374301317185186
1203	10	0.1703392967990709
1203	11	0.20681786621437936
1203	12	0.2445808704798651
1203	13	0.408850552298496
1203	14	0.4469504713989849
1203	15	0.7532046970724267
1203	16	0.6532467286250139
1203	17	0.46302229912097204
1203	18	0.4817917274923076
1203	19	0.34905792050408024
1203	20	-0.2099568161392753
1203	21	0.11847883155311223
1203	22	0.13445675389782963
1203	23	0.01798098733174669
1203	24	0.2868516973927413
1203	25	0.13229603449917704
1204	1	0.5667303517922626
1204	2	0.5784649734743326
1204	3	0.7686985389080476
1204	4	0.5430414649386535
1204	5	0.2569251415128946
1204	6	0.39903000216404294
1204	7	-0.03348536751492759
1204	8	0.31159253765290273
1204	9	-0.4111417534334656
1204	10	-0.23132541794076644
1204	11	-0.08803636667838077
1204	12	0.30646287650294823
1204	13	0.3573249123453479
1204	14	-1.3794810577962622
1204	15	0.016952187452218936
1204	16	0.14371958289973463
1204	17	0.03259838244036217
1204	18	0.16598634853965422
1204	19	0.3857590247369771
1204	20	-0.6625762322343007
1204	21	-0.616329600770797
1204	22	-1.4131873401292694
1204	23	-0.981595514257684
1204	24	-0.11756314282810365
1204	25	-2.5266235740000695
1205	1	-0.526362695738932
1205	2	-0.5100005797615772
1205	3	-0.2410889908292937
1205	4	-0.08880166351047336
1205	5	-0.0859742087161095
1205	6	-0.21437395229534317
1205	7	-0.17326806240576786
1205	8	-5.387800147182986E-4
1205	9	0.17929364573469897
1205	10	0.029145362593617108
1205	11	-0.37903495466044745
1205	12	-0.041731540968847014
1205	13	-0.2931940750476407
1205	14	0.014451209847891278
1205	15	-0.5429518641086943
1205	16	-1.1402148695286094
1205	17	-1.3463337233679304
1205	18	-1.2178918659252318
1205	19	-0.9502031299457485
1205	20	-0.4534301794110789
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2111	19	-0.2786366715637527
2111	20	-0.3490707091805021
2111	21	-0.04250976464428646
2111	22	-0.025755923771015432
2111	23	0.04853027965604895
2111	24	0.15214834124513743
2111	25	0.23330842819784436
2112	1	-1.0079892407164834
2112	2	-0.761987715429683
2112	3	-0.38167715172155425
2112	4	-0.38347425925277534
2112	5	-0.9291545163780945
2112	6	-0.9193299603163645
2112	7	-0.43653304272508464
2112	8	-0.0124992547386924
2112	9	-2.0187439230790076
2112	10	-0.9208033098780106
2112	11	-1.200707440973499
2112	12	-0.7989288826434375
2112	13	-0.32707071749301875
2112	14	-1.0867258414649505
2112	15	-1.0062724591195717
2112	16	-1.0940722031594383
2112	17	-0.9390101117100684
2112	18	-0.3287057968843783
2112	19	-0.03383407767409352
2112	20	-0.08258440501439424
2112	21	-0.10803017227173939
2112	22	-0.04666239435061925
2112	23	0.06164353857053584
2112	24	-0.06263236409870387
2112	25	0.16658549877527662
2113	1	-0.23479595321843405
2113	2	-0.6052434413984642
2113	3	-0.36246669797358066
2113	4	-0.4404795039777447
2113	5	-0.2551820468546495
2113	6	-0.6665860379761277
2113	7	-0.4229150084378759
2113	8	-0.16897309972023677
2113	9	-1.1193048968367343
2113	10	-0.23238353472044793
2113	11	-0.4355324414064512
2113	12	-0.7201194631866663
2113	13	-0.03767833027059453
2113	14	0.0862794778611331
2113	15	-1.6938676255000438
2113	16	-0.19119286646994738
2113	17	-1.4623968958557292
2113	18	0.03171218251771535
2113	19	0.09215721797412613
2113	20	0.4199014107821242
2113	21	0.37585398817927995
2113	22	0.31327667252261904
2113	23	0.49984050949874614
2113	24	0.4352488309579101
2113	25	0.6471265966639308
2114	1	-0.8144775377969573
2114	2	-0.8019867399213076
2114	3	-2.7273824760886924
2114	4	-1.577953518036061
2114	5	-1.00330587710625
2114	6	-1.645867393329688
2114	7	-3.102623417214268
2114	8	-1.413306487921247
2114	9	-1.0621800240992627
2114	10	-0.8850238892875737
2114	11	-0.7500278721482054
2114	12	-0.546370293822342
2114	13	-2.604574221900833
2114	14	-2.491189110393524
2114	15	-1.3379638634062943
2114	16	-1.6793963158179324
2114	17	-1.4066879140169313
2114	18	-0.31750229523343876
2114	19	-0.6763606231656567
2114	20	-0.3255140899463953
2114	21	-0.3757478431818697
2114	22	-0.30430476025533437
2114	23	0.15871139366088727
2114	24	-0.18846711297951657
2114	25	-0.2947944426518134
2201	1	1.3327187238852858
2201	2	1.5646601323245584
2201	3	1.5636874123556055
2201	4	1.4841114170900838
2201	5	1.166405359997924
2201	6	1.0686428597089908
2201	7	1.0458759771707165
2201	8	1.232548937399585
2201	9	1.4247426975393402
2201	10	1.2288564770034682
2201	11	1.0077190514688965
2201	12	1.4617937265187848
2201	13	1.3895534332615043
2201	14	1.893808839470033
2201	15	1.6491591589189838
2201	16	1.6827779599183472
2201	17	1.4684304990360744
2201	18	1.4565443913219056
2201	19	1.3288530858846386
2201	20	1.3064310418043235
2201	21	0.9756997416631492
2201	22	1.0214265728989105
2201	23	1.0650755355963142
2201	24	0.9926930757272956
2201	25	1.2974251717340195
2202	1	1.1802572984628021
2202	2	1.037961667081973
2202	3	1.0192853808334448
2202	4	0.7496082207440651
2202	5	0.8405509168490823
2202	6	0.5553750502647894
2202	7	0.3491409076247649
2202	8	0.4930164009203395
2202	9	0.7883519285377254
2202	10	0.9162703990884395
2202	11	0.6082630583340105
2202	12	0.886091291826844
2202	13	0.8906349998323151
2202	14	-1.2342673170447611
2202	15	0.4294710902767491
2202	16	0.7696962288338156
2202	17	1.0355721064463097
2202	18	0.8452903187735785
2202	19	0.7767644841290107
2202	20	0.6093291511192476
2202	21	0.41454287671668766
2202	22	0.6599532413515163
2202	23	0.4143754074312298
2202	24	0.7253543853232216
2202	25	1.0686071472548235
2203	1	1.0054124508225541
2203	2	0.9586310251348493
2203	3	1.0744315046679382
2203	4	1.0180173295228805
2203	5	0.7190952908306656
2203	6	0.2888453930505115
2203	7	0.561242384995154
2203	8	0.6644228174091893
2203	9	1.0357329783952487
2203	10	0.9170355191569755
2203	11	0.8187746476526954
2203	12	0.8016835278373691
2203	13	0.6684459755908634
2203	14	0.7540172902686137
2203	15	1.0969621634240667
2203	16	0.8338567793298424
2203	17	1.1654885180277645
2203	18	1.0040275181955352
2203	19	0.8854061113241158
2203	20	0.1088544079441931
2203	21	0.40169580041344943
2203	22	-0.393879543853636
2203	23	0.055218503591802914
2203	24	0.37416752738557335
2203	25	0.6055923789734123
2204	1	0.5511791657954781
2204	2	0.36829034767248103
2204	3	0.6692775514890599
2204	4	0.6499215516299621
2204	5	0.7236632566813839
2204	6	0.45703848481749176
2204	7	0.2537863011684607
2204	8	0.18668007847075074
2204	9	0.689699570805292
2204	10	-1.378282105437659
2204	11	-0.3359296891269494
2204	12	0.28281804980269243
2204	13	0.4012999285067238
2204	14	0.6246924118452029
2204	15	0.5255845408809137
2204	16	0.5241061921589676
2204	17	0.26632252422955105
2204	18	0.7236778194740339
2204	19	0.446425329499192
2204	20	-0.6700700370379664
2204	21	0.0103051851530509
2204	22	0.13650631880755526
2204	23	0.10831926553313309
2204	24	0.44239454750324825
2204	25	-0.21243698877571404
2205	1	0.296673042229326
2205	2	0.31106668653643155
2205	3	0.5461804662689005
2205	4	0.360865216245859
2205	5	0.4694939254379946
2205	6	0.18991078914344328
2205	7	0.38960009469644774
2205	8	0.43612515970027843
2205	9	0.6641486330014708
2205	10	0.6289197557723654
2205	11	0.36822878030965
2205	12	0.5067076144808098
2205	13	0.41700248899325487
2205	14	0.9093175370612343
2205	15	0.1948748299168983
2205	16	0.20230367150754347
2205	17	0.6390137543507919
2205	18	-0.007856981805780094
2205	19	-0.5268729408025905
2205	20	-0.01311540276034151
2205	21	-0.48358803743563783
2205	22	-1.3933733160932817
2205	23	-1.4535398687884964
2205	24	-0.9867170279267867
2205	25	-1.7929916582588206
2206	1	0.1562959596576441
2206	2	0.04208285965319547
2206	3	0.6264280246331353
2206	4	0.37778692721885676
2206	5	0.14814158129356514
2206	6	-0.061474110144519756
2206	7	0.07313450391634646
2206	8	-0.038217208991074614
2206	9	0.35805020156748313
2206	10	-0.0711533627318417
2206	11	-0.08105135060728941
2206	12	0.3115926269283662
2206	13	0.5756400475315786
2206	14	0.5504832549282597
2206	15	0.1527777215796533
2206	16	-0.47274028664082124
2206	17	-0.10063214317884928
2206	18	0.5654321322416749
2206	19	-0.3272420151571467
2206	20	-0.0228889094889837
2206	21	-2.2313135296798556
2206	22	-1.1397200022582155
2206	23	-1.7930969795630318
2206	24	-0.6470606848965446
2206	25	-1.495679633746711
2207	1	0.5264729213332373
2207	2	0.6022225288806453
2207	3	0.7792424305475123
2207	4	0.45490530753630765
2207	5	0.5019863071149047
2207	6	0.39765216547015214
2207	7	0.4130674539510153
2207	8	0.3261562178190829
2207	9	-1.1173755493320705
2207	10	-0.11761167217726154
2207	11	0.29898053634691024
2207	12	0.32326194174105183
2207	13	0.639735555511507
2207	14	0.7928110025845143
2207	15	0.7935127595546092
2207	16	0.5323998466947018
2207	17	0.474490572453945
2207	18	0.7182050414348424
2207	19	-0.5922531810512055
2207	20	0.2836451860735991
2207	21	-0.5017099871702868
2207	22	0.08045450237467122
2207	23	-0.6988803120773497
2207	24	-1.3756251713473233
2207	25	-0.22495884661942966
2208	1	0.8378207647016112
2208	2	0.4234904836784139
2208	3	0.725954115557311
2208	4	0.8972773453373257
2208	5	0.5645604049205772
2208	6	0.7700403248977885
2208	7	0.7654586286882079
2208	8	1.0129006771877869
2208	9	0.5135141554417153
2208	10	0.7337018720449962
2208	11	0.805399205224802
2208	12	0.731979013190184
2208	13	1.232013450551328
2208	14	1.575787095976878
2208	15	1.3229411528057966
2208	16	0.9174351737872541
2208	17	1.0672892936885816
2208	18	1.1293673404713118
2208	19	-0.5853046914471705
2208	20	0.29264742699682955
2208	21	0.3268953636335681
2208	22	0.6188487301127807
2208	23	0.8079897966108547
2208	24	0.6842878301192172
2208	25	0.7243998004659957
2209	1	0.06077766046440303
2209	2	-1.534683649203437
2209	3	-0.4048657572348553
2209	4	-0.16272025235646126
2209	5	-0.02572341039927295
2209	6	0.017209035431328346
2209	7	0.26292476761014427
2209	8	0.2569349500897715
2209	9	-1.7280119953773578
2209	10	-0.313000104709829
2209	11	-0.03455572293480458
2209	12	0.20922860136626298
2209	13	0.243744712562858
2209	14	0.5702246131362045
2209	15	0.7156038232551438
2209	16	0.5534343043811987
2209	17	0.4891971824046024
2209	18	0.3396549308437855
2209	19	0.3693460589875315
2209	20	0.3189869986999483
2209	21	0.1836607155142289
2209	22	0.11699838235035287
2209	23	0.4459279315060698
2209	24	0.382586768528661
2209	25	0.2886586318012192
2210	1	0.4719825275662686
2210	2	0.3381275501614489
2210	3	0.22393912244350034
2210	4	0.6541298869674748
2210	5	0.5152920626143356
2210	6	0.4664923719742866
2210	7	0.5849355912523748
2210	8	0.4295417814822642
2210	9	0.7365977600491966
2210	10	-0.8814680916460027
2210	11	0.021307869303932137
2210	12	-1.694766792494054
2210	13	-0.17993878433035704
2210	14	-0.08588264860807726
2210	15	-0.2208518858820696
2210	16	0.27192528271426397
2210	17	0.30102379976094795
2210	18	0.6257693061655658
2210	19	0.5590415900101959
2210	20	0.6399571177442951
2210	21	0.5150229459548399
2210	22	0.5957181742094377
2210	23	0.5391091155040542
2210	24	0.38167890311282804
2210	25	0.23788812524376368
2211	1	-0.4177667130079179
2211	2	0.011717400658799448
2211	3	-1.2876220187799206
2211	4	-0.6104520813848211
2211	5	-0.44754868628871947
2211	6	-0.4268068120152293
2211	7	-0.5122550295906407
2211	8	0.11577284547257705
2211	9	0.27324477098889943
2211	10	0.3412154114413397
2211	11	0.052195156615063354
2211	12	-0.03661165123092047
2211	13	-0.06792298033170852
2211	14	-0.509900358616612
2211	15	-0.03880014565532974
2211	16	0.06198226446818467
2211	17	-0.16019485531082722
2211	18	0.1628352421951078
2211	19	-0.011210757757190493
2211	20	-0.032487141393630736
2211	21	-0.3106962749636182
2211	22	-0.21776088946320726
2211	23	-0.46146070203520395
2211	24	-0.19180770518228485
2211	25	0.028568531065033653
2212	1	-0.44975962388286916
2212	2	-0.4442698699633745
2212	3	-2.3743654843012862
2212	4	-1.1166151786942713
2212	5	-0.6304537183169892
2212	6	-0.339730882052681
2212	7	-0.25952556552245554
2212	8	-0.35639479249908845
2212	9	-0.7919484443217115
2212	10	-0.12205330477589627
2212	11	-0.2125284688428266
2212	12	-0.05482000855042202
2212	13	-0.18162150709595792
2212	14	0.1182099195366817
2212	15	0.14709749755555634
2212	16	-1.1551601368219977
2212	17	-1.0240707180043032
2212	18	-0.12766623578397684
2212	19	2.4901749199557344E-5
2212	20	0.08324196261254002
2212	21	0.35559221879135094
2212	22	-0.16348898012121538
2212	23	0.13301461000056491
2212	24	-0.10518670386493056
2212	25	0.2049497569575891
2213	1	-3.2931362692747683
2213	2	-1.5533749718061038
2213	3	-2.6285740329113807
2213	4	-1.171177071313057
2213	5	-0.8887229433024295
2213	6	-0.5916933220493625
2213	7	-0.1977295535614374
2213	8	-0.1925541791733636
2213	9	-0.2668616576742302
2213	10	-0.12770024694213333
2213	11	-0.2330004569656552
2213	12	0.035045297843581125
2213	13	-0.16811113416628132
2213	14	-0.1184088963735519
2213	15	-0.020197265352159377
2213	16	-1.1045276811221498
2213	17	-0.7525845860958924
2213	18	-0.07285599072162796
2213	19	-0.1870008931836793
2213	20	-0.050101882029103706
2213	21	0.1001546673550564
2213	22	-0.020018735478110727
2213	23	0.1270313667272731
2213	24	0.1732200309065064
2213	25	0.1993511182023937
2214	1	-0.8160982126028848
2214	2	-0.8946047650063633
2214	3	-6.661612757393613
2214	4	-2.019291103337288
2214	5	-1.477295549790071
2214	6	-0.9287280055975486
2214	7	-0.5400649817522343
2214	8	-0.6255644535718652
2214	9	-0.5469868039706967
2214	10	-0.4007699428328877
2214	11	-0.8330595718102956
2214	12	-0.50200880671985
2214	13	-5.112042322442804
2214	14	-3.983103194261261
2214	15	-2.651615357220379
2214	16	-2.5289441562760295
2214	17	-3.1668978743293117
2214	18	-1.2241141828976865
2214	19	-0.6124327126431979
2214	20	-0.5093816349392739
2214	21	-0.122100112514552
2214	22	-0.5489364568137987
2214	23	-0.37162364278731985
2214	24	-0.25199543162882065
2214	25	-0.3396195339178192
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	286.0
3	40.0
4	40.0
5	70.0
6	6.0
7	17.0
8	32.0
9	57.0
10	181.0
11	729.0
12	1920.0
13	4035.0
14	7120.0
15	11305.0
16	15413.0
17	18223.0
18	18612.0
19	17220.0
20	15766.0
21	14315.0
22	12596.0
23	10957.0
24	9868.0
25	9127.0
26	8943.0
27	8855.0
28	9414.0
29	10737.0
30	13311.0
31	16419.0
32	21494.0
33	28689.0
34	39008.0
35	54002.0
36	76653.0
37	117303.0
38	189525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.12204614810885	21.73191972140955	13.29318703482906	17.85284709565254
2	16.475913972284857	25.775632830813734	37.20281298973348	20.545640207167924
3	19.48371566165748	34.22644634824714	26.40185229180421	19.88798569829117
4	11.64784770884712	23.74430542230109	32.27708295872604	32.33076391012575
5	11.446735850715749	44.369094758173006	28.543735702407968	15.640433688703279
6	24.518479138362178	42.47635549779799	16.83357289595106	16.171592467888765
7	23.02851704787845	42.53759065402159	17.50218225970531	16.931710038394645
8	23.65376866019382	38.92306743199768	18.305595990806577	19.117567917001928
9	24.447565665979706	24.561636103490372	18.136937031543297	32.85386119898662
10	17.338769488050218	36.336345008551966	23.91334881394778	22.411536689450035
11	27.053881997768592	35.4434599986874	17.568418980114195	19.934239023429807
12	20.685151592316558	37.2612050204372	24.120229022663374	17.93341436458287
13	26.785894768620917	31.50220845447565	22.809889150680256	18.90200762622318
14	21.131768863033155	32.958623532160544	24.685036372753117	21.224571232053176
15	24.72187983633736	33.60111628737312	23.547572823180552	18.129431053108974
16	18.49688979545762	35.70638199008462	24.15603132912514	21.64069688533262
17	18.462770321268007	33.83756111967971	24.53424342860893	23.165425130443342
18	18.54903125114952	30.255337708949693	29.22602563361482	21.969605406285964
19	19.917248295805837	31.722493000103704	30.586757364494744	17.773501339595718
20	22.610674878515567	26.938959814970875	30.999992124185837	19.450373182327723
21	24.045668261923076	28.303375240535438	29.39756537444772	18.253391123093763
22	23.178250716386916	28.1395992958865	28.64077478738109	20.04137520034549
23	22.327190149904176	28.4812685411252	29.97020293507653	19.221338373894095
24	21.545241633540403	28.395419911464927	29.684193390851377	20.375145064143293
25	21.05721910602777	29.48892806557179	30.2558050698945	19.198047758505943
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3437.0
1	3437.0
2	3963.5
3	4490.0
4	4490.0
5	4490.0
6	6247.0
7	8004.0
8	8004.0
9	8004.0
10	9135.0
11	10266.0
12	10266.0
13	10266.0
14	11212.0
15	12158.0
16	12158.0
17	12158.0
18	16368.0
19	20578.0
20	20578.0
21	20578.0
22	26861.0
23	33144.0
24	33144.0
25	33144.0
26	43566.0
27	53988.0
28	53988.0
29	53988.0
30	61027.0
31	68066.0
32	68066.0
33	68066.0
34	70608.5
35	73151.0
36	73151.0
37	73151.0
38	66761.5
39	60372.0
40	60372.0
41	60372.0
42	64790.0
43	69208.0
44	69208.0
45	69208.0
46	85937.0
47	102666.0
48	102666.0
49	102666.0
50	95702.5
51	88739.0
52	88739.0
53	88739.0
54	74642.0
55	60545.0
56	60545.0
57	60545.0
58	49402.5
59	38260.0
60	38260.0
61	38260.0
62	34666.5
63	31073.0
64	31073.0
65	31073.0
66	21763.5
67	12454.0
68	12454.0
69	12454.0
70	9884.0
71	7314.0
72	7314.0
73	7314.0
74	5137.0
75	2960.0
76	2960.0
77	2960.0
78	2014.0
79	1068.0
80	1068.0
81	1068.0
82	651.5
83	235.0
84	235.0
85	235.0
86	154.5
87	74.0
88	74.0
89	74.0
90	48.5
91	23.0
92	23.0
93	23.0
94	15.0
95	7.0
96	7.0
97	7.0
98	7.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09707617068614487
2	0.051686501689650104
3	0.055228470079549986
4	0.04971874147303906
5	0.04709506118422434
6	0.06336187897487564
7	0.06073819868606091
8	0.05758978233948324
9	0.06270595890267196
10	0.06231240685934975
11	0.057458598325042506
12	0.057983334382805445
13	0.058114518397246184
14	0.05955754255609428
15	0.06257477488823122
16	0.05785215036836471
17	0.06073819868606091
18	0.14430241588480994
19	0.06441135109040153
20	0.06060701467162018
21	0.0585080704405684
22	0.06283714291711269
23	0.061394118758264594
24	0.07293831202904939
25	0.06519845517704595
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	762288.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.22275640838793
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.99397286018939	45.43065053675581
2	8.406757382500114	8.780480859414407
3	2.0935482633450353	3.2799258295761415
4	0.7877578246564793	1.6455553994334755
5	0.38921639234525524	1.0162976423798904
6	0.22949170328718355	0.7190813591107575
7	0.16689485659220082	0.6100996609139139
8	0.11111426557788566	0.46421545798166775
9	0.08629911605213776	0.4056099944264992
>10	0.5501712198689698	5.522321770151774
>50	0.07128164355195049	2.61593075592324
>100	0.08922839338040867	10.156808734260684
>500	0.012133039326506488	4.227042476747621
>1k	0.011374724368599834	9.958406234646198
>5k	0.0	0.0
>10k+	7.583149579066555E-4	5.167573288278031
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	17552	2.3025418214638034	No Hit
GGTATCAACGCAGAGTACTTTTTTT	11418	1.4978590768843272	No Hit
TATCAACGCAGAGTACTTTTTTTTT	10177	1.3350597149633734	No Hit
GTACTGGTTCACTATCGGTCAGTCA	4684	0.6144659236404089	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4303	0.5644848141384883	No Hit
ATACAGGGTGACAGCCCCGTACACA	2889	0.3789906177192872	No Hit
GTTAATGATAGTGTGTCGAAACACA	2703	0.35459039103331025	No Hit
GGATACCACGTGTCCCGCCCTACTC	2662	0.3492118464412401	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2656	0.34842474235459564	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	2551	0.3346504208383183	No Hit
CCCATGTACTCTGCGTTGATACCAC	2259	0.29634468862162333	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2256	0.29595113657830113	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2195	0.2879489116974162	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	1964	0.2576454043616061	No Hit
GGTATCAACGCAGAGTACATGGGGG	1872	0.24557647503305838	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	1856	0.24347753080200657	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	1750	0.22957202527128856	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1731	0.22707952899691455	No Hit
AGCGTACACGGTGGATGCCCTGGCA	1534	0.2012362781520895	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	1528	0.20044917406544507	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1501	0.1969072056755452	No Hit
TCATTAACCTATGGATTCAGTTAAT	1485	0.19480826144449342	No Hit
GTGGTATCAACGCAGAGTACATGGG	1474	0.19336523728564534	No Hit
GTACTTAGATGTTTCAGTTCCCCCG	1464	0.19205339714123798	No Hit
GTATCAACGCAGAGTACATGGGGGT	1415	0.18562538043364188	No Hit
GTACATGGGGGTTAAGCGACTAAGC	1398	0.18339525218814937	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	1362	0.17867262766828285	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	1357	0.17801670759607918	No Hit
GTGGATGCCCTGGCAGTCAGAGGCG	1336	0.1752618432928237	No Hit
CCCATATTCAGACAGGATACCACGT	1302	0.17080158680183868	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	1228	0.16109396973322418	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	1220	0.1600444976176983	No Hit
ATCATTAACTGAATCCATAGGTTAA	1216	0.15951976155993536	No Hit
GTGGTATCAACGCAGAGTACTTTTT	1203	0.15781436937220578	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	1163	0.15256700879457633	No Hit
GGGTACTTAGATGTTTCAGTTCCCC	1148	0.15059924857796528	No Hit
TATTCAGACAGGATACCACGTGTCC	1144	0.15007451252020235	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	1113	0.14600780807253952	No Hit
GGGTTTCCCCATTCGGAAATCGCCG	1104	0.1448271519425729	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1080	0.14167873559599523	No Hit
CCTATGGATTCAGTTAATGATAGTG	1074	0.1408916315093508	No Hit
GGTATCAACGCAGAGTACATGGGAA	1066	0.1398421593938249	No Hit
CGGTACTGGTTCACTATCGGTCAGT	1054	0.13826795122053606	No Hit
GTGCTAATCTGCGATAAGCGTCGGT	1048	0.13748084713389166	No Hit
TCACGGTACTGGTTCACTATCGGTC	1031	0.13525071888839912	No Hit
ACGCAGAGTACATGGGGGTTAAGCG	1024	0.134332430787314	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	1022	0.1340700627584325	No Hit
GGTATCAACGCAGAGTACATGGGGA	1015	0.13315177465734734	No Hit
ATGATAGTGTGTCGAAACACACTGG	963	0.12633020590642904	No Hit
CACACACACTGATTCAGGCTCTGGG	958	0.12567428583422538	No Hit
GGTTCACTATCGGTCAGTCAGGAGT	934	0.12252586948764772	No Hit
CATGTACTCTGCGTTGATACCACTG	916	0.12016455722771446	No Hit
CATCTAAGTACCCCGAGGAAAAGAA	856	0.11229351636127029	No Hit
GTCCCGCCCTACTCATCGAGCTCAC	849	0.11137522826018512	No Hit
TAATGATAGTGTGTCGAAACACACT	825	0.10822681191360746	No Hit
AGCTCACAGCATGTGCATTTTTGTG	814	0.10678378775475934	No Hit
GTATCAACGCAGAGTACATGGGAAG	790	0.10363537140818167	No Hit
GTTATAACCGGCGATTTCCGAATGG	776	0.10179879520601139	No Hit
TATCAACGCAGAGTACATGGGGGTT	763	0.1000934030182818	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.3118401444073632E-4	0.0	0.0	0.0	0.0
9	1.3118401444073632E-4	0.0	0.0	0.0	0.0
10	1.3118401444073632E-4	0.0	0.0	1.3118401444073632E-4	0.0
11	1.3118401444073632E-4	0.0	0.0	1.3118401444073632E-4	0.0
12	1.3118401444073632E-4	0.0	0.0	1.3118401444073632E-4	0.0
13	1.3118401444073632E-4	0.0	0.0	1.3118401444073632E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAGA	40	1.2880961E-5	18.992647	1
GTACAAG	260	0.0	15.340214	1
CAAGGAC	50	8.7432214E-5	15.1921215	4
TCTGAAC	40	0.005294008	14.242615	3
GTGGGTC	40	0.005294008	14.242615	11
GTACAAA	215	0.0	14.134063	1
GTATCAC	55	1.9645267E-4	13.812834	1
GTGTTAC	50	0.0015027003	13.294852	1
ATCGCCG	180	0.0	13.191071	19
AATCGCC	185	0.0	12.851433	18
CCGAATG	195	0.0	12.680081	18
CGAATGG	195	0.0	12.663427	19
TCCAAGA	75	1.4841295E-5	12.660102	2
GGTATCA	3200	0.0	12.641979	1
CATCGCC	95	1.0275271E-6	12.009548	16
AAATCGC	200	0.0	11.884449	17
GAAATCG	205	0.0	11.594584	16
TACAAGA	230	0.0	11.559224	2
TACATGA	215	0.0	11.482418	2
GTATAAA	100	1.9341242E-6	11.395587	1
>>END_MODULE