FastQCFastQC Report
Wed 25 May 2016
SRR1294608_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294608_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762288
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT369344.8451503893541545No Hit
GGTATCAACGCAGAGTACTTTTTTT262473.443186827026006No Hit
TATCAACGCAGAGTACTTTTTTTTT235833.0937126125558843No Hit
ACGCAGAGTACTTTTTTTTTTTTTT132831.7425172638163005No Hit
GTACTTTTTTTTTTTTTTTTTTTTT75630.9921447012152886No Hit
GAGTACTTTTTTTTTTTTTTTTTTT65470.8588617425435007No Hit
GCAGAGTACTTTTTTTTTTTTTTTT48620.63781667821086No Hit
GTACTGGTTCACTATCGGTCAGTCA44090.5783903196692064No Hit
ATCAACGCAGAGTACTTTTTTTTTT38350.5030906953802238No Hit
ATACAGGGTGACAGCCCCGTACACA31380.41165543731503057No Hit
GTGGTATCAACGCAGAGTACTTTTT28280.37098839283840224No Hit
GGATACCACGTGTCCCGCCCTACTC26880.3526226308166992No Hit
GTTAATGATAGTGTGTCGAAACACA26450.3469817181957476No Hit
CCCATGTACTCTGCGTTGATACCAC26010.3412096215603551No Hit
ACTTAGATGTTTCAGTTCCCCCGGT22180.2909661440295531No Hit
GTACATGGGAAGCAGTGGTATCAAC21330.27981550280209055No Hit
ACCCTGTATCGCGCGCCTTTCCAGA20550.2695831496757131No Hit
GGTATCAACGCAGAGTACATGGGGG19680.2581701404193691No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA19440.2550217240727914No Hit
AGCGTACACGGTGGATGCCCTGGCA17220.22589887286694793No Hit
CAACGCAGAGTACTTTTTTTTTTTT16490.2163224398127742No Hit
GTTCACTATCGGTCAGTCAGGAGTA16410.21527296769724827No Hit
CCCATATTCAGACAGGATACCACGT15140.1986125978632748No Hit
AACGCAGAGTACTTTTTTTTTTTTT14670.19244694918456015No Hit
GTGGTATCAACGCAGAGTACATGGG14580.19126629305459353No Hit
CTATCGGTCAGTCAGGAGTATTTAG14540.1907415569968306No Hit
GTATCAACGCAGAGTACATGGGGGT14430.18929853283798248No Hit
GTACTTAGATGTTTCAGTTCCCCCG14420.18916734882354175No Hit
TCATTAACCTATGGATTCAGTTAAT14330.18798669269357512No Hit
GCCTTGGAGGATGGTCCCCCCATAT13720.17998446781269023No Hit
GTCAGGAGTATTTAGCCTTGGAGGA13580.17814789161051992No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG13220.1734252670906534No Hit
GTGGATGCCCTGGCAGTCAGAGGCG12970.170145666729635No Hit
GTATTTAGCCTTGGAGGATGGTCCC12880.16896501059966837No Hit
GCCCAGAGCCTGAATCAGTGTGTGT12790.16778435446970175No Hit
TCAACGCAGAGTACTTTTTTTTTTT12630.16568541023864997No Hit
CGGTACTGGTTCACTATCGGTCAGT12290.1612251537476649No Hit
GTACATGGGGGTTAAGCGACTAAGC12270.16096278571878345No Hit
CCTATGGATTCAGTTAATGATAGTG12100.15873265747329093No Hit
GGGTACTTAGATGTTTCAGTTCCCC11870.155715425141154No Hit
ACGCAGAGTACATGGGGGTTAAGCG11790.1546659530256281No Hit
GAGTACATGGGAAGCAGTGGTATCA11690.15335411288122075No Hit
ATCATTAACTGAATCCATAGGTTAA11470.15046806456352455No Hit
GGGTTTCCCCATTCGGAAATCGCCG11310.14836912033247276No Hit
TCACGGTACTGGTTCACTATCGGTC11260.14771320026026907No Hit
GGTATCAACGCAGAGTACATGGGAA11180.1466637281447432No Hit
CACACACACTGATTCAGGCTCTGGG11020.1445647839136914No Hit
TATTCAGACAGGATACCACGTGTCC10970.1439088638414877No Hit
GGTATCAACGCAGAGTACATGGGGA10870.14259702369708036No Hit
CATGTACTCTGCGTTGATACCACTG10590.13892387129273975No Hit
GGTTAATGAGGCGAACCGGGGGAAC10490.1376120311483324No Hit
GGTTCACTATCGGTCAGTCAGGAGT9970.1307904623974141No Hit
TCTAAGTACCCCGAGGAAAAGAAAT9870.12947862225300674No Hit
GTGCTAATCTGCGATAAGCGTCGGT9860.129347438238566No Hit
ATGATAGTGTGTCGAAACACACTGG9460.12410007766093654No Hit
CATCTAAGTACCCCGAGGAAAAGAA9340.12252586948764772No Hit
AGCTCACAGCATGTGCATTTTTGTG9280.12173876540100328No Hit
CCCCAGTAGCGGCGAGCGAACGGGG8770.11504838066452575No Hit
CATTAACCTATGGATTCAGTTAATG8380.10993220410133703No Hit
GCCTGAATCAGTGTGTGTGTTAGTG8300.10888273198581115No Hit
TAATGATAGTGTGTCGAAACACACT8250.10822681191360746No Hit
ACGCAGAGTACATGGGAAGCAGTGG8240.10809562789916671No Hit
GGGTGACAGCCCCGTACACAAAAAT8040.10547194761035199No Hit
GTATCAACGCAGAGTACATGGGAAG7970.10455365950926684No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT7930.10402892345150389No Hit
GTCCCGCCCTACTCATCGAGCTCAC7870.10324181936485947No Hit
GCGTTGATACCACTGCTTCCCATGT7670.10061813907604475No Hit
GTTATAACCGGCGATTTCCGAATGG7630.1000934030182818No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGT402.7287917E-416.6488421
CATGCTA350.002197762316.2534415
AAGGCGA1004.1836756E-1115.16987819
GTACATA1650.014.9911661
GTACAAA2150.014.6023071
TCTTACT400.005267113414.2535512
GGTATCA54050.013.9580111
CGCAGCG551.9214909E-413.8489388
AATACTG500.00147342113.3296035
CATCTTA957.560811E-812.97423913
ACAGGGT6300.012.8206543
CAACCCC1001.47631E-712.32552612
AATCGCC1700.012.26975518
ACGCAGC550.003015328412.1178217
CTACACA550.003015328412.1178214
CCTAATA550.003060254712.0939212
CTAGGAG550.003060254712.0939213
GAGTACC550.003099653612.07328511
AGGGTGA6600.011.973565
ATCGCCG1750.011.9191919
Produced by FastQC (version 0.11.5)