FastQCFastQC Report
Wed 25 May 2016
SRR1294607_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294607_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742229
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC164092.2107732249750414No Hit
GTACATGGGAAGCAGTGGTATCAAC162042.183153716710072No Hit
GTATCAACGCAGAGTACTTTTTTTT161832.18032440122927No Hit
GGTATCAACGCAGAGTACTTTTTTT101781.3712749030285802No Hit
TATCAACGCAGAGTACTTTTTTTTT94811.277368574927684No Hit
GAGTACATGGGAAGCAGTGGTATCA76721.033643255652905No Hit
CATGTACTCTGCGTTGATACCACTG65710.8853062868737277No Hit
GCGTTGATACCACTGCTTCCCATGT50030.6740507309738639No Hit
ACGCAGAGTACATGGGAAGCAGTGG47290.6371349004148316No Hit
GTACTGGTTCACTATCGGTCAGTCA46310.62393142817109No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA46280.6235272402452612No Hit
GTATCAACGCAGAGTACATGGGAAG44130.5945604388941957No Hit
TATCAACGCAGAGTACATGGGAAGC43900.5914616647961748No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43630.5878239734637154No Hit
GGTATCAACGCAGAGTACATGGGAA40430.5447105947086411No Hit
ACTCTGCGTTGATACCACTGCTTCC39960.5383783172039895No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34280.4618520699137328No Hit
GTTAATGATAGTGTGTCGAAACACA31380.4227805704169468No Hit
GTGGTATCAACGCAGAGTACATGGG30660.4130800601970551No Hit
GGATACCACGTGTCCCGCCCTACTC26110.3517782247796839No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25880.34867945068166295No Hit
ATACAGGGTGACAGCCCCGTACACA25340.34140406801674417No Hit
GCTTCCCATGTACTCTGCGTTGATA23900.32200304757696074No Hit
ACTTAGATGTTTCAGTTCCCCCGGT21950.2957308323980874No Hit
ATACCACTGCTTCCCATGTACTCTG21320.28724288595568215No Hit
GTACTCTGCGTTGATACCACTGCTT21090.2841441118576612No Hit
CAGTGGTATCAACGCAGAGTACATG20190.27201847408279656No Hit
CATGGGAAGCAGTGGTATCAACGCA18070.24345586065755986No Hit
ACATGGGAAGCAGTGGTATCAACGC17760.23927925209066203No Hit
GATACCACTGCTTCCCATGTACTCT17560.2365846659184699No Hit
GGGAAGCAGTGGTATCAACGCAGAG17550.23644993660986033No Hit
GTTCACTATCGGTCAGTCAGGAGTA17400.2344289969807162No Hit
AGCGTACACGGTGGATGCCCTGGCA17320.23335116251183932No Hit
GTACATGGGGGTTAAGCGACTAAGC16890.22755780224162622No Hit
GCAGAGTACTTTTTTTTTTTTTTTT16720.2252674039952629No Hit
ACCCTGTATCGCGCGCCTTTCCAGA16600.22365065229194764No Hit
TCATTAACCTATGGATTCAGTTAAT16590.22351592298333803No Hit
GCAGAGTACATGGGAAGCAGTGGTA16580.22338119367472845No Hit
GGTATCAACGCAGAGTACATGGGGG16140.21745310409590574No Hit
GTACTTAGATGTTTCAGTTCCCCCG15920.21448905930649434No Hit
TACCACTGCTTCCCATGTACTCTGC15850.2135459541462271No Hit
CTGCTTCCCATGTACTCTGCGTTGA15760.21233339036874063No Hit
GTATTTAGCCTTGGAGGATGGTCCC15250.20546219562965068No Hit
GCAGTGGTATCAACGCAGAGTACAT15210.20492327839521227No Hit
GTTGATACCACTGCTTCCCATGTAC15140.20398017323494502No Hit
ATCAACGCAGAGTACTTTTTTTTTT15010.20222869222302015No Hit
GTGGATGCCCTGGCAGTCAGAGGCG14760.19886045950777992No Hit
GTATCAACGCAGAGTACATGGGGGT14490.19522276817532055No Hit
CTATCGGTCAGTCAGGAGTATTTAG14170.19091143029981314No Hit
CTTCCCATGTACTCTGCGTTGATAC13610.18336658901767514No Hit
CCACTGCTTCCCATGTACTCTGCGT13170.17743849943885243No Hit
GTACATGGTAAGCAGTGGTATCAAC13160.17730377013024284No Hit
GTCAGGAGTATTTAGCCTTGGAGGA12780.17218405640307774No Hit
AAGCAGTGGTATCAACGCAGAGTAC12740.17164513916863933No Hit
GGGTACTTAGATGTTTCAGTTCCCC12730.17151040986002972No Hit
CCTATGGATTCAGTTAATGATAGTG12700.17110622193420089No Hit
CCCATATTCAGACAGGATACCACGT12700.17110622193420089No Hit
ATCATTAACTGAATCCATAGGTTAA12680.1708367633169817No Hit
CCATGTACTCTGCGTTGATACCACT12500.16841163576200877No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA12360.16652542544147428No Hit
ATGGGAAGCAGTGGTATCAACGCAG12330.16612123751564542No Hit
GCCTTGGAGGATGGTCCCCCCATAT12300.16571704958981662No Hit
TATTCAGACAGGATACCACGTGTCC11310.15237884803746554No Hit
GTGGTATCAACGCAGAGTACTTTTT11100.14954953255666378No Hit
TTCCCATGTACTCTGCGTTGATACC11040.14874115670500612No Hit
GGGTTTCCCCATTCGGAAATCGCCG11030.14860642739639654No Hit
GTGCTAATCTGCGATAAGCGTCGGT10950.14752859292751969No Hit
TGATACCACTGCTTCCCATGTACTC10640.14335198436062185No Hit
GCCCAGAGCCTGAATCAGTGTGTGT10630.14321725505201224No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG10500.14146577404008737No Hit
ACGCAGAGTACATGGGGGTTAAGCG10400.1401184809539913No Hit
CGGTACTGGTTCACTATCGGTCAGT10370.13971429302816246No Hit
GAACAAAAAAAAAAAAAAAAAAAAA10350.13944483441094324No Hit
ATGATAGTGTGTCGAAACACACTGG10240.13796281201623758No Hit
TCACGGTACTGGTTCACTATCGGTC9860.1328430982890725No Hit
GGTTAATGAGGCGAACCGGGGGAAC9770.13163053451158604No Hit
GGTTCACTATCGGTCAGTCAGGAGT9410.1267802794016402No Hit
AAACAAAAAAAAAAAAAAAAAAAAA9370.12624136216720178No Hit
ACCATGTACTCTGCGTTGATACCAC9020.12152583636586552No Hit
ATCAACGCAGAGTACATGGGAAGCA8850.1192354381195022No Hit
CACACACACTGATTCAGGCTCTGGG8790.11842706226784455No Hit
TCTAAGTACCCCGAGGAAAAGAAAT8710.1173492277989677No Hit
TAATGATAGTGTGTCGAAACACACT8670.11681031056452928No Hit
GGTATCAACGCAGAGTACATGGGGA8040.10832236412212402No Hit
TATCAACGCAGAGTACATGGGGGTT7710.10387629693800701No Hit
GATATGAACCGTTATAACCGGCGAT7660.10320265039495896No Hit
GTCCCGCCCTACTCATCGAGCTCAC7560.1018553573088629No Hit
GTTATAACCGGCGATTTCCGAATGG7500.10104698145720525No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC250.006040055318.9940663
TACATAA1300.016.8024442
TACAAGA1100.015.5406022
GTACAAA2400.015.0400121
CTGTGCA456.7793764E-414.7711739
TACATGA1550.014.7050862
TTCTATA400.005286600414.2455512
CTTAGGA400.005286600414.2455512
ACTGTGC500.001503315413.2940568
GTACATA2250.013.087451
AATCGCC1750.013.03768718
ATCGCCG1900.012.99506519
ACAGGGT4300.012.8099533
CCGAATG1800.012.67552918
TGCTTGG751.4665551E-512.67467314
GCTTGGT751.4665551E-512.67467315
GTACAAG2100.012.6652731
TCCAAGA604.0983263E-412.6627132
TCCAAAA905.4126576E-712.6627112
AGGGTGA4400.012.5171315
Produced by FastQC (version 0.11.5)