##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294607_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 742229 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.300493513457436 31.0 26.0 31.0 15.0 34.0 2 27.131091617277146 31.0 26.0 31.0 12.0 34.0 3 27.35925435411443 31.0 26.0 33.0 15.0 34.0 4 29.941954571971724 35.0 27.0 37.0 10.0 37.0 5 31.46952220945288 35.0 30.0 37.0 17.0 37.0 6 30.85593664488992 35.0 29.0 37.0 13.0 37.0 7 30.96334554429967 35.0 30.0 37.0 13.0 37.0 8 30.541245356891203 35.0 28.0 37.0 13.0 37.0 9 30.80902255233897 37.0 27.0 39.0 10.0 39.0 10 31.349103848003782 37.0 28.0 39.0 12.0 39.0 11 31.54328246403738 37.0 28.0 39.0 12.0 39.0 12 31.541103891117164 37.0 28.0 39.0 12.0 39.0 13 30.99242147639071 37.0 27.0 39.0 10.0 39.0 14 31.586899730406653 37.0 27.0 40.0 10.0 41.0 15 31.515699332685735 36.0 27.0 40.0 10.0 41.0 16 31.7272553349438 36.0 27.0 40.0 11.0 41.0 17 31.993636734754368 37.0 27.0 40.0 12.0 41.0 18 31.96731466973131 37.0 27.0 40.0 12.0 41.0 19 32.30334034374836 37.0 28.0 40.0 13.0 41.0 20 32.48837353431353 37.0 28.0 40.0 13.0 41.0 21 32.581963248539196 37.0 28.0 40.0 15.0 41.0 22 32.782988808036336 37.0 29.0 40.0 16.0 41.0 23 32.91129152862526 37.0 29.0 40.0 17.0 41.0 24 33.10235116116455 37.0 29.0 40.0 18.0 41.0 25 31.879232689641608 35.0 27.0 40.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2418301643124252 1101 2 1.0427200987168952 1101 3 1.1157498141286162 1101 4 1.0996804883031146 1101 5 0.613042426001865 1101 6 0.794474044531686 1101 7 1.0340080980761854 1101 8 0.9087826197238051 1101 9 1.0380494493861683 1101 10 1.1187256394147482 1101 11 0.7714699456919547 1101 12 0.9035755055182264 1101 13 0.8212902462728238 1101 14 1.0216158321208546 1101 15 1.2203131909709413 1101 16 1.2701574170126086 1101 17 1.3119749346979965 1101 18 1.1764584480744986 1101 19 1.1145163615570937 1101 20 0.9879988836947788 1101 21 0.9786109596329098 1101 22 0.6460412404380094 1101 23 0.9668725440153025 1101 24 0.9420568400200011 1101 25 0.7386141716833272 1102 1 1.0874467346399754 1102 2 0.9565481653744854 1102 3 0.966136408344969 1102 4 1.2002491366261552 1102 5 0.7117925961940905 1102 6 0.6633718796865935 1102 7 0.44861182868974225 1102 8 0.8493066261106534 1102 9 1.1094125962713406 1102 10 1.192436407528355 1102 11 0.9314351645900985 1102 12 1.0443286009537758 1102 13 0.9814947657531299 1102 14 1.0218499753628798 1102 15 1.0657162474170008 1102 16 1.16917463427691 1102 17 0.9446578242937811 1102 18 1.1528811519235234 1102 19 0.8090893956821787 1102 20 0.23591841268522273 1102 21 0.42176675668951447 1102 22 0.4999564867732076 1102 23 0.7105321100560786 1102 24 0.5998513344562681 1102 25 0.7592357962042122 1103 1 0.7246805226275335 1103 2 0.645416694827091 1103 3 0.8555211314916491 1103 4 0.8530722406237601 1103 5 0.5926945055573647 1103 6 0.6985293126144647 1103 7 0.295641409140174 1103 8 0.6120496775563176 1103 9 0.7380544288665583 1103 10 0.7120037558432983 1103 11 0.45087543722891255 1103 12 0.7717717717378463 1103 13 0.606899755420546 1103 14 0.45909534363382676 1103 15 0.8709481784599973 1103 16 0.5248640633740642 1103 17 0.9875586750346947 1103 18 0.9420390347402758 1103 19 0.45790859107793835 1103 20 -0.5116836744302802 1103 21 0.07133303408443936 1103 22 -0.351846629962715 1103 23 0.3595419189660305 1103 24 0.5513694020041626 1103 25 0.5617642947179924 1104 1 0.26033746769668653 1104 2 0.2405450733329353 1104 3 0.3459703147722095 1104 4 0.3522878404565546 1104 5 0.1570436172489984 1104 6 0.17004168578241874 1104 7 0.03479360163793288 1104 8 0.18051788303738903 1104 9 0.35583261042839 1104 10 0.5526474008615132 1104 11 0.6367330993788229 1104 12 0.6775603470983498 1104 13 0.5863087545755796 1104 14 0.5509193123213656 1104 15 0.676801690239401 1104 16 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-0.46866146763091265 2207 10 0.2064058167250522 2207 11 0.8973913453313145 2207 12 0.7925316451578155 2207 13 0.8597380474628729 2207 14 1.0158422425525053 2207 15 1.0722246246282339 2207 16 0.9756512563290052 2207 17 1.0153788523599125 2207 18 0.8392117002435739 2207 19 0.02374425858428708 2207 20 0.5972520721526209 2207 21 -0.34854045301898395 2207 22 0.5652476687088068 2207 23 -0.43165089978945304 2207 24 -1.2048086636269808 2207 25 -0.02237167283808361 2208 1 0.785509526891147 2208 2 0.3753014463833253 2208 3 0.6822653114044783 2208 4 0.752292552508596 2208 5 0.6662639943794666 2208 6 0.5152812659108612 2208 7 0.5608720379018841 2208 8 0.6923174157126972 2208 9 0.004284424152654509 2208 10 0.15897762701229112 2208 11 0.47654966196457593 2208 12 0.5046224935212109 2208 13 0.9599384482644773 2208 14 1.2703512605885798 2208 15 1.1443689132053905 2208 16 0.7137942089008185 2208 17 0.976634697383286 2208 18 1.3001335439309472 2208 19 -0.878059348630142 2208 20 0.44895547896624777 2208 21 0.4717668282101428 2208 22 0.592635778261247 2208 23 0.9945348049533678 2208 24 0.8018713630931273 2208 25 1.048436610395047 2209 1 0.10903739337538099 2209 2 -1.3774333689181297 2209 3 -0.4203678023665738 2209 4 -0.08683359580035699 2209 5 -0.015470481401791858 2209 6 -0.050864906142432886 2209 7 0.3826694110133886 2209 8 0.24189420754266777 2209 9 -2.192486292870729 2209 10 -0.664538097982529 2209 11 -0.3548333066604883 2209 12 0.11068899974361202 2209 13 0.059057152736652796 2209 14 0.13740901566692187 2209 15 0.5411735380973894 2209 16 0.6188406975600067 2209 17 0.3117084580352376 2209 18 0.6947469611156194 2209 19 0.18101804828751256 2209 20 0.40626600219380293 2209 21 0.41261834839084344 2209 22 0.35578225186498713 2209 23 0.3787480430621031 2209 24 0.44948428004843066 2209 25 0.5656397763656749 2210 1 -0.13072719726199722 2210 2 -0.10532278190408917 2210 3 -0.025743636441333706 2210 4 0.07695445492979047 2210 5 0.12907423859376976 2210 6 -0.14506219263929054 2210 7 -0.017560906929439568 2210 8 0.07733642358313375 2210 9 0.2786596765007694 2210 10 -1.3461936350520673 2210 11 -0.6221530568541063 2210 12 -1.9976894389535857 2210 13 -0.7265179605409884 2210 14 -0.5555134377953941 2210 15 -0.6751408501947544 2210 16 -0.201730616503621 2210 17 -0.12816914148073266 2210 18 -0.044937861078157226 2210 19 -0.031956385818809 2210 20 0.40180326953763057 2210 21 0.2569137866249136 2210 22 0.171751937276305 2210 23 0.04026452072844222 2210 24 0.09438656047987593 2210 25 -0.14035911160631542 2211 1 -0.26549444183061155 2211 2 0.10746760425688251 2211 3 -1.178319956494402 2211 4 -0.48381455013482366 2211 5 -0.38874121549938323 2211 6 -0.4223275555183399 2211 7 -0.30148553728906435 2211 8 0.23782221842156304 2211 9 0.4296923732489546 2211 10 0.3252059592684198 2211 11 0.3168929584290403 2211 12 0.12668067343749811 2211 13 -0.03344385386513693 2211 14 -0.4999940276891266 2211 15 -0.04835324510717598 2211 16 0.26712263722467355 2211 17 0.16986497131858513 2211 18 0.4078540559489028 2211 19 0.34265930300713876 2211 20 0.36539417109018757 2211 21 0.0894497938710117 2211 22 0.1978787185027855 2211 23 -0.23364569397552515 2211 24 -0.024408647909268666 2211 25 0.15748364293206052 2212 1 -0.30886237724006804 2212 2 -0.4955391968408378 2212 3 -1.9643279905396618 2212 4 -1.1769856116628823 2212 5 -0.6328648284334015 2212 6 -0.634108170401209 2212 7 -0.489808330909554 2212 8 -0.26471128991077464 2212 9 -0.7181579230561788 2212 10 -0.26131116279338684 2212 11 -0.23374936746683517 2212 12 -0.17768236995430087 2212 13 -0.653481240016351 2212 14 -0.10234060526538968 2212 15 -0.17634910876704168 2212 16 -1.1108354425589617 2212 17 -1.255470112004808 2212 18 -0.04617290980697675 2212 19 -0.018196601220672903 2212 20 -0.09677820293041606 2212 21 -0.07868447809335066 2212 22 -0.29409916798649505 2212 23 0.19296210938922798 2212 24 -0.19119065482968978 2212 25 0.1695665288247561 2213 1 -2.943728325312062 2213 2 -1.4255981946253122 2213 3 -2.5549928980600143 2213 4 -1.4051278037915331 2213 5 -0.8828063285205303 2213 6 -0.43701325118087553 2213 7 -0.28447861544317377 2213 8 -0.3784351205348564 2213 9 -0.4940335547307697 2213 10 -0.232623801263923 2213 11 -0.8304907978519367 2213 12 -0.4154185477940331 2213 13 -0.3286598514524641 2213 14 -0.4215932999954184 2213 15 -0.4238908677474029 2213 16 -1.7359306605022553 2213 17 -1.223474393700556 2213 18 -0.4597747618893244 2213 19 -0.16349544864792165 2213 20 0.08533327052057871 2213 21 0.2942761153695912 2213 22 0.041217353882515795 2213 23 0.15439697533729912 2213 24 0.23547285865018353 2213 25 0.1950401291832229 2214 1 -1.291212377730183 2214 2 -1.1445967068636804 2214 3 -5.863630067644461 2214 4 -2.5317098242941114 2214 5 -1.4252523716859962 2214 6 -0.926900548142175 2214 7 -0.48340253579697645 2214 8 -0.3797578017666403 2214 9 -0.6867700171189348 2214 10 -0.7559816588871726 2214 11 -1.0925895607679514 2214 12 -0.914560537318561 2214 13 -5.064457841955246 2214 14 -3.7122958088098947 2214 15 -2.0273758984197485 2214 16 -2.7181508899843934 2214 17 -3.2418125843378505 2214 18 -1.0570397261219036 2214 19 -0.6187484700268548 2214 20 -0.3424715677617698 2214 21 -0.4895561955522112 2214 22 -0.28216292206669635 2214 23 -0.559278203499801 2214 24 -0.27728316882244997 2214 25 -0.10958008399467545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 274.0 3 46.0 4 46.0 5 67.0 6 15.0 7 11.0 8 35.0 9 111.0 10 424.0 11 1106.0 12 2605.0 13 5132.0 14 9116.0 15 14419.0 16 19243.0 17 22022.0 18 21561.0 19 20396.0 20 18587.0 21 16627.0 22 14433.0 23 13074.0 24 12015.0 25 11166.0 26 10681.0 27 10925.0 28 11549.0 29 12973.0 30 15512.0 31 18943.0 32 23855.0 33 30288.0 34 38815.0 35 51643.0 36 69971.0 37 101184.0 38 143359.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.80060176862253 20.51421412011906 11.61958296975917 18.065601141499243 2 14.91336383415247 28.00678854412958 33.760875015839034 23.318972605878912 3 21.448503263621337 37.00752479732116 23.61090515951438 17.93306677954312 4 11.99416588932444 25.664964601722197 27.46534311796011 34.87552639099326 5 10.69549639897934 46.795610569464046 27.014866425924655 15.49402660563196 6 25.48788034443847 38.99901446485172 17.30578149162295 18.207323699086864 7 21.50971935931364 44.20579508120475 16.469592203481685 17.81489335599993 8 25.73446841782433 38.25854852081775 18.01184963500698 17.995133426350947 9 25.587393090952233 26.15429415761602 16.136120967654957 32.122191783776785 10 18.147856543884295 38.41166652735573 21.793444116988834 21.647032811771144 11 25.6497327464765 37.55663550393972 14.258464151630147 22.535167597953638 12 22.25140136267185 39.85828644149597 21.890781338891152 15.999530856941025 13 28.236141031067923 31.671865774055867 19.835426007759565 20.25656718711664 14 21.657146631902926 36.02792783198004 22.36154382294218 19.953381713174856 15 29.29656797649879 31.131016017547715 21.396591030119353 18.175824975834143 16 17.254400250210306 35.370381355018225 25.887195056189473 21.488023338582 17 21.20182216008747 34.45427545678706 22.896582034278072 21.4473203488474 18 21.575553157042634 28.43051807879115 28.20372369131139 21.790205072854828 19 20.536856651724023 33.55151841989956 29.74768276652398 16.163942161852436 20 23.827736360327325 26.88596195586233 28.871617886946087 20.414683796864256 21 22.53827197774051 29.427632394890242 29.687137703627414 18.34695792374184 22 23.253926525109538 28.924165824064712 24.40701044826424 23.41489720256151 23 20.76242681287689 29.631701769972352 28.6780278876137 20.927843529537064 24 21.56583591510749 32.07183668626928 26.09438912392246 20.267938274700768 25 21.706597926747225 28.454713488546417 26.245293117604312 23.593395467102045 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5889.0 1 5889.0 2 6334.0 3 6779.0 4 6779.0 5 6779.0 6 8801.5 7 10824.0 8 10824.0 9 10824.0 10 11066.5 11 11309.0 12 11309.0 13 11309.0 14 11073.0 15 10837.0 16 10837.0 17 10837.0 18 13249.0 19 15661.0 20 15661.0 21 15661.0 22 19709.0 23 23757.0 24 23757.0 25 23757.0 26 31571.5 27 39386.0 28 39386.0 29 39386.0 30 44241.5 31 49097.0 32 49097.0 33 49097.0 34 51037.5 35 52978.0 36 52978.0 37 52978.0 38 47369.5 39 41761.0 40 41761.0 41 41761.0 42 51220.0 43 60679.0 44 60679.0 45 60679.0 46 106948.0 47 153217.0 48 153217.0 49 153217.0 50 136347.0 51 119477.0 52 119477.0 53 119477.0 54 90584.0 55 61691.0 56 61691.0 57 61691.0 58 46950.5 59 32210.0 60 32210.0 61 32210.0 62 29329.0 63 26448.0 64 26448.0 65 26448.0 66 18677.0 67 10906.0 68 10906.0 69 10906.0 70 8412.5 71 5919.0 72 5919.0 73 5919.0 74 4166.5 75 2414.0 76 2414.0 77 2414.0 78 1590.5 79 767.0 80 767.0 81 767.0 82 469.0 83 171.0 84 171.0 85 171.0 86 106.0 87 41.0 88 41.0 89 41.0 90 22.5 91 4.0 92 4.0 93 4.0 94 3.0 95 2.0 96 2.0 97 2.0 98 3.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10077752283998605 2 0.052679159666356334 3 0.05591266307298691 4 0.0518707838146987 5 0.047963633865020096 6 0.0673646543048035 7 0.062244940577638436 8 0.05847251993656945 9 0.06426588020678255 10 0.06507425605844018 11 0.05712522685047337 12 0.06076291818293276 13 0.05860724924517905 14 0.06197548196041923 15 0.06467006813261136 16 0.06103237680015198 17 0.06359223366373451 18 0.1386364585592856 19 0.06790357153924192 20 0.06184075265180961 21 0.05833779062795983 22 0.06453533882400175 23 0.06278385781207686 24 0.06615209052731705 25 0.06682573707036507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 742229.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.53231903015259 #Duplication Level Percentage of deduplicated Percentage of total 1 87.04099566563974 31.798094223592805 2 6.829198616247767 4.989729251380801 3 2.187838509620223 2.3978044325969865 4 0.9783618918348234 1.4296731503781366 5 0.5907521877801494 1.0790773695872513 6 0.3924644086404205 0.860258099065921 7 0.2640936449030872 0.6753567302604788 8 0.21178033468128452 0.6189461400711341 9 0.14976568582301378 0.4924159032830347 >10 1.037030887033396 7.200448467570794 >50 0.13356068179193212 3.4583096423889765 >100 0.13876917632230465 11.14134307676154 >500 0.017857695612521776 4.472765258656959 >1k 0.02455433146721744 17.46606067108533 >5k 0.0014881413010434815 3.9043765409291815 >10k+ 0.0014881413010434815 8.015341042390697 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 16409 2.2107732249750414 No Hit GTACATGGGAAGCAGTGGTATCAAC 16204 2.183153716710072 No Hit GTATCAACGCAGAGTACTTTTTTTT 16183 2.18032440122927 No Hit GGTATCAACGCAGAGTACTTTTTTT 10178 1.3712749030285802 No Hit TATCAACGCAGAGTACTTTTTTTTT 9481 1.277368574927684 No Hit GAGTACATGGGAAGCAGTGGTATCA 7672 1.033643255652905 No Hit CATGTACTCTGCGTTGATACCACTG 6571 0.8853062868737277 No Hit GCGTTGATACCACTGCTTCCCATGT 5003 0.6740507309738639 No Hit ACGCAGAGTACATGGGAAGCAGTGG 4729 0.6371349004148316 No Hit GTACTGGTTCACTATCGGTCAGTCA 4631 0.62393142817109 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 4628 0.6235272402452612 No Hit GTATCAACGCAGAGTACATGGGAAG 4413 0.5945604388941957 No Hit TATCAACGCAGAGTACATGGGAAGC 4390 0.5914616647961748 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4363 0.5878239734637154 No Hit GGTATCAACGCAGAGTACATGGGAA 4043 0.5447105947086411 No Hit ACTCTGCGTTGATACCACTGCTTCC 3996 0.5383783172039895 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3428 0.4618520699137328 No Hit GTTAATGATAGTGTGTCGAAACACA 3138 0.4227805704169468 No Hit GTGGTATCAACGCAGAGTACATGGG 3066 0.4130800601970551 No Hit GGATACCACGTGTCCCGCCCTACTC 2611 0.3517782247796839 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2588 0.34867945068166295 No Hit ATACAGGGTGACAGCCCCGTACACA 2534 0.34140406801674417 No Hit GCTTCCCATGTACTCTGCGTTGATA 2390 0.32200304757696074 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 2195 0.2957308323980874 No Hit ATACCACTGCTTCCCATGTACTCTG 2132 0.28724288595568215 No Hit GTACTCTGCGTTGATACCACTGCTT 2109 0.2841441118576612 No Hit CAGTGGTATCAACGCAGAGTACATG 2019 0.27201847408279656 No Hit CATGGGAAGCAGTGGTATCAACGCA 1807 0.24345586065755986 No Hit ACATGGGAAGCAGTGGTATCAACGC 1776 0.23927925209066203 No Hit GATACCACTGCTTCCCATGTACTCT 1756 0.2365846659184699 No Hit GGGAAGCAGTGGTATCAACGCAGAG 1755 0.23644993660986033 No Hit GTTCACTATCGGTCAGTCAGGAGTA 1740 0.2344289969807162 No Hit AGCGTACACGGTGGATGCCCTGGCA 1732 0.23335116251183932 No Hit GTACATGGGGGTTAAGCGACTAAGC 1689 0.22755780224162622 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1672 0.2252674039952629 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 1660 0.22365065229194764 No Hit TCATTAACCTATGGATTCAGTTAAT 1659 0.22351592298333803 No Hit GCAGAGTACATGGGAAGCAGTGGTA 1658 0.22338119367472845 No Hit GGTATCAACGCAGAGTACATGGGGG 1614 0.21745310409590574 No Hit GTACTTAGATGTTTCAGTTCCCCCG 1592 0.21448905930649434 No Hit TACCACTGCTTCCCATGTACTCTGC 1585 0.2135459541462271 No Hit CTGCTTCCCATGTACTCTGCGTTGA 1576 0.21233339036874063 No Hit GTATTTAGCCTTGGAGGATGGTCCC 1525 0.20546219562965068 No Hit GCAGTGGTATCAACGCAGAGTACAT 1521 0.20492327839521227 No Hit GTTGATACCACTGCTTCCCATGTAC 1514 0.20398017323494502 No Hit ATCAACGCAGAGTACTTTTTTTTTT 1501 0.20222869222302015 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 1476 0.19886045950777992 No Hit GTATCAACGCAGAGTACATGGGGGT 1449 0.19522276817532055 No Hit CTATCGGTCAGTCAGGAGTATTTAG 1417 0.19091143029981314 No Hit CTTCCCATGTACTCTGCGTTGATAC 1361 0.18336658901767514 No Hit CCACTGCTTCCCATGTACTCTGCGT 1317 0.17743849943885243 No Hit GTACATGGTAAGCAGTGGTATCAAC 1316 0.17730377013024284 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 1278 0.17218405640307774 No Hit AAGCAGTGGTATCAACGCAGAGTAC 1274 0.17164513916863933 No Hit GGGTACTTAGATGTTTCAGTTCCCC 1273 0.17151040986002972 No Hit CCTATGGATTCAGTTAATGATAGTG 1270 0.17110622193420089 No Hit CCCATATTCAGACAGGATACCACGT 1270 0.17110622193420089 No Hit ATCATTAACTGAATCCATAGGTTAA 1268 0.1708367633169817 No Hit CCATGTACTCTGCGTTGATACCACT 1250 0.16841163576200877 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 1236 0.16652542544147428 No Hit ATGGGAAGCAGTGGTATCAACGCAG 1233 0.16612123751564542 No Hit GCCTTGGAGGATGGTCCCCCCATAT 1230 0.16571704958981662 No Hit TATTCAGACAGGATACCACGTGTCC 1131 0.15237884803746554 No Hit GTGGTATCAACGCAGAGTACTTTTT 1110 0.14954953255666378 No Hit TTCCCATGTACTCTGCGTTGATACC 1104 0.14874115670500612 No Hit GGGTTTCCCCATTCGGAAATCGCCG 1103 0.14860642739639654 No Hit GTGCTAATCTGCGATAAGCGTCGGT 1095 0.14752859292751969 No Hit TGATACCACTGCTTCCCATGTACTC 1064 0.14335198436062185 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 1063 0.14321725505201224 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 1050 0.14146577404008737 No Hit ACGCAGAGTACATGGGGGTTAAGCG 1040 0.1401184809539913 No Hit CGGTACTGGTTCACTATCGGTCAGT 1037 0.13971429302816246 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 1035 0.13944483441094324 No Hit ATGATAGTGTGTCGAAACACACTGG 1024 0.13796281201623758 No Hit TCACGGTACTGGTTCACTATCGGTC 986 0.1328430982890725 No Hit GGTTAATGAGGCGAACCGGGGGAAC 977 0.13163053451158604 No Hit GGTTCACTATCGGTCAGTCAGGAGT 941 0.1267802794016402 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 937 0.12624136216720178 No Hit ACCATGTACTCTGCGTTGATACCAC 902 0.12152583636586552 No Hit ATCAACGCAGAGTACATGGGAAGCA 885 0.1192354381195022 No Hit CACACACACTGATTCAGGCTCTGGG 879 0.11842706226784455 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 871 0.1173492277989677 No Hit TAATGATAGTGTGTCGAAACACACT 867 0.11681031056452928 No Hit GGTATCAACGCAGAGTACATGGGGA 804 0.10832236412212402 No Hit TATCAACGCAGAGTACATGGGGGTT 771 0.10387629693800701 No Hit GATATGAACCGTTATAACCGGCGAT 766 0.10320265039495896 No Hit GTCCCGCCCTACTCATCGAGCTCAC 756 0.1018553573088629 No Hit GTTATAACCGGCGATTTCCGAATGG 750 0.10104698145720525 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.3472930860960702E-4 0.0 11 0.0 0.0 0.0 1.3472930860960702E-4 0.0 12 0.0 0.0 0.0 2.6945861721921403E-4 0.0 13 0.0 0.0 0.0 2.6945861721921403E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTATAC 25 0.0060400553 18.994066 3 TACATAA 130 0.0 16.802444 2 TACAAGA 110 0.0 15.540602 2 GTACAAA 240 0.0 15.040012 1 CTGTGCA 45 6.7793764E-4 14.771173 9 TACATGA 155 0.0 14.705086 2 TTCTATA 40 0.0052866004 14.245551 2 CTTAGGA 40 0.0052866004 14.245551 2 ACTGTGC 50 0.0015033154 13.294056 8 GTACATA 225 0.0 13.08745 1 AATCGCC 175 0.0 13.037687 18 ATCGCCG 190 0.0 12.995065 19 ACAGGGT 430 0.0 12.809953 3 CCGAATG 180 0.0 12.675529 18 TGCTTGG 75 1.4665551E-5 12.674673 14 GCTTGGT 75 1.4665551E-5 12.674673 15 GTACAAG 210 0.0 12.665273 1 TCCAAGA 60 4.0983263E-4 12.662713 2 TCCAAAA 90 5.4126576E-7 12.662711 2 AGGGTGA 440 0.0 12.517131 5 >>END_MODULE