FastQCFastQC Report
Wed 25 May 2016
SRR1294607_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294607_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742229
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT359544.8440575617498105No Hit
GGTATCAACGCAGAGTACTTTTTTT242863.272035988892916No Hit
TATCAACGCAGAGTACTTTTTTTTT226253.0482506072923585No Hit
CCCATGTACTCTGCGTTGATACCAC192382.59192243903162No Hit
GTACATGGGAAGCAGTGGTATCAAC161812.180054942612051No Hit
ACGCAGAGTACTTTTTTTTTTTTTT139131.8744888706854625No Hit
GTACTTTTTTTTTTTTTTTTTTTTT91141.2279229186679583No Hit
GAGTACATGGGAAGCAGTGGTATCA82261.1082832926226271No Hit
CATGTACTCTGCGTTGATACCACTG79271.0679992293483547No Hit
GAGTACTTTTTTTTTTTTTTTTTTT71050.9572517376712578No Hit
ACGCAGAGTACATGGGAAGCAGTGG57110.7694390814694656No Hit
GCGTTGATACCACTGCTTCCCATGT55350.7457267231541748No Hit
GCAGAGTACTTTTTTTTTTTTTTTT49460.6663711603831163No Hit
TATCAACGCAGAGTACATGGGAAGC46960.6326888332307146No Hit
ACTCTGCGTTGATACCACTGCTTCC46110.6212368419988978No Hit
GTATCAACGCAGAGTACATGGGAAG46050.6204284661472403No Hit
GTACTGGTTCACTATCGGTCAGTCA44630.6012969043246761No Hit
GGTATCAACGCAGAGTACATGGGAA43450.5853988459087425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA43440.5852641166001329No Hit
ATCAACGCAGAGTACTTTTTTTTTT39950.53824358789538No Hit
GTGGTATCAACGCAGAGTACATGGG32750.44123848569646296No Hit
GTTAATGATAGTGTGTCGAAACACA30380.4093076395559861No Hit
ATACAGGGTGACAGCCCCGTACACA29470.39704727247251187No Hit
GCTTCCCATGTACTCTGCGTTGATA28040.37778098134133803No Hit
GTGGTATCAACGCAGAGTACTTTTT26300.3543380816432664No Hit
GGATACCACGTGTCCCGCCCTACTC25550.3442333834975459No Hit
ACTTAGATGTTTCAGTTCCCCCGGT25400.34221244386840177No Hit
CAGTGGTATCAACGCAGAGTACATG25080.33790110599289436No Hit
CATGGGAAGCAGTGGTATCAACGCA23360.31472766491204196No Hit
ATACCACTGCTTCCCATGTACTCTG22680.3055660719265887No Hit
ACATGGGAAGCAGTGGTATCAACGC21980.2961350203239162No Hit
GGGAAGCAGTGGTATCAACGCAGAG21460.28912909627621663No Hit
AGCGTACACGGTGGATGCCCTGGCA21350.28764707388151095No Hit
GTACTCTGCGTTGATACCACTGCTT20900.28158425499407863No Hit
GATACCACTGCTTCCCATGTACTCT19360.26083594146819916No Hit
GCAGAGTACATGGGAAGCAGTGGTA18990.2558509570496437No Hit
GCAGTGGTATCAACGCAGAGTACAT18730.2523479950257939No Hit
CTGCTTCCCATGTACTCTGCGTTGA18710.2520785364085747No Hit
ACCCTGTATCGCGCGCCTTTCCAGA18410.2480366571502865No Hit
CAACGCAGAGTACTTTTTTTTTTTT17650.23779722969595635No Hit
GGTATCAACGCAGAGTACATGGGGG17300.2330817038946201No Hit
TCATTAACCTATGGATTCAGTTAAT16790.22621050915553015No Hit
GTTCACTATCGGTCAGTCAGGAGTA16480.22203390058863234No Hit
CTATCGGTCAGTCAGGAGTATTTAG16460.22176444197141315No Hit
CCATGTACTCTGCGTTGATACCACT16400.2209560661197555No Hit
TACCACTGCTTCCCATGTACTCTGC16330.22001296095948822No Hit
GTTGATACCACTGCTTCCCATGTAC16210.21839620925617298No Hit
CTTCCCATGTACTCTGCGTTGATAC16020.21583635239259044No Hit
GTGGATGCCCTGGCAGTCAGAGGCG15770.21246811967735027No Hit
GTACTTAGATGTTTCAGTTCCCCCG15730.2119292024429118No Hit
CCACTGCTTCCCATGTACTCTGCGT15400.2074831352587948No Hit
CCCATATTCAGACAGGATACCACGT15350.20680948871574675No Hit
GTACATGGGGGTTAAGCGACTAAGC14910.20088139913692404No Hit
AAGCAGTGGTATCAACGCAGAGTAC14790.19926464743360875No Hit
GTATCAACGCAGAGTACATGGGGGT14760.19886045950777992No Hit
AACGCAGAGTACTTTTTTTTTTTTT14640.19724370780446468No Hit
ATGGGAAGCAGTGGTATCAACGCAG14250.19198926476869No Hit
GTCAGGAGTATTTAGCCTTGGAGGA13860.1867348217329153No Hit
CCTATGGATTCAGTTAATGATAGTG13790.18579171657264806No Hit
GCCTTGGAGGATGGTCCCCCCATAT13530.18228875454879828No Hit
GTATTTAGCCTTGGAGGATGGTCCC13110.1766301235871948No Hit
ATCATTAACTGAATCCATAGGTTAA13070.17609120635275635No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG12870.17339662018056423No Hit
GTACATGGTAAGCAGTGGTATCAAC12790.17231878571168738No Hit
GGGTACTTAGATGTTTCAGTTCCCC12790.17231878571168738No Hit
TCAACGCAGAGTACTTTTTTTTTTT12720.17137568055142013No Hit
CGGTACTGGTTCACTATCGGTCAGT12560.1692200116136664No Hit
GCCCAGAGCCTGAATCAGTGTGTGT12060.16248354618318606No Hit
ACGCAGAGTACATGGGGGTTAAGCG12030.16207935825735723No Hit
TCACGGTACTGGTTCACTATCGGTC11610.15642072729575374No Hit
ATGATAGTGTGTCGAAACACACTGG11230.1513010135685887No Hit
GGGTTTCCCCATTCGGAAATCGCCG11010.14833696877917732No Hit
GTGCTAATCTGCGATAAGCGTCGGT10960.14766332223612927No Hit
TATTCAGACAGGATACCACGTGTCC10880.14658548776725241No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA10680.14389090159506027No Hit
TTCCCATGTACTCTGCGTTGATACC10490.14133104473147776No Hit
GGTTCACTATCGGTCAGTCAGGAGT10470.14106158611425854No Hit
ACCATGTACTCTGCGTTGATACCAC10310.1389059171765048No Hit
ATCAACGCAGAGTACATGGGAAGCA10300.13877118786789522No Hit
CACACACACTGATTCAGGCTCTGGG10220.13769335339901836No Hit
TGATACCACTGCTTCCCATGTACTC9940.13392093275794936No Hit
GGTTAATGAGGCGAACCGGGGGAAC9550.12866648972217468No Hit
TAATGATAGTGTGTCGAAACACACT9330.12570244493276334No Hit
TCTAAGTACCCCGAGGAAAAGAAAT9050.12193002429169435No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT8940.12044800189698866No Hit
CATTAACCTATGGATTCAGTTAATG8930.12031327258837905No Hit
GGTATCAACGCAGAGTACATGGGGA8730.1176186864161869No Hit
GCCTGAATCAGTGTGTGTGTTAGTG8460.11398099508372753No Hit
AGCTCACAGCATGTGCATTTTTGTG8200.11047803305987774No Hit
CATCTAAGTACCCCGAGGAAAAGAA8070.10872655204795287No Hit
CCCCAGTAGCGGCGAGCGAACGGGG8040.10832236412212402No Hit
GAGTACATGGGGGTTAAGCGACTAA7920.10670561241880876No Hit
TATCAACGCAGAGTACATGGGGGTT7830.10549304864132228No Hit
GATATGAACCGTTATAACCGGCGAT7650.10306792108634937No Hit
GTTATAACCGGCGATTTCCGAATGG7530.10145116938303407No Hit
AAAAAGTACTCTGCGTTGATACCAC7510.10118171076581486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA2300.016.13151
GCTAAAA508.599711E-515.2215191
GTACATA2200.014.7026041
TACATAA1650.014.3977752
TAGGACC400.005207636414.2778944
TTAGGAC400.005266334414.2537983
CTTAGGA400.005266334414.2537982
TAACAAC954.789399E-914.0198211
CGCAAAA903.615969E-813.725882
AATCGCC1950.013.12987318
TACATGA957.340532E-813.0034652
GAATAAA1251.0004442E-1012.93829251
AAATCGC1850.012.81444217
ACGAAGA751.44672085E-512.6914627
GCTTGGT751.5040623E-512.64358115
ACATAAA3050.012.4623373
AACCAGA1155.1932147E-912.4088471
ATCGCCG2000.012.32749219
TACAAAA3200.012.1751182
CATGAAA1103.6992787E-812.1145764
Produced by FastQC (version 0.11.5)