FastQCFastQC Report
Wed 25 May 2016
SRR1294606_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294606_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4573822
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC616821.3485876800627572No Hit
CCCATGTACTCTGCGTTGATACCAC586021.2812479366271796No Hit
GAGTACATGGGAAGCAGTGGTATCA279150.61032108376758No Hit
CATGTACTCTGCGTTGATACCACTG216500.4733459238247575No Hit
GCGTTGATACCACTGCTTCCCATGT190030.4154730988656752No Hit
GTATCAACGCAGAGTACATGGGAAG171990.3760312491391226No Hit
GTATCAACGCAGAGTACTTTTTTTT167580.3663894222381195No Hit
ACGCAGAGTACATGGGAAGCAGTGG147780.32309958717239107No Hit
TATCAACGCAGAGTACATGGGAAGC134200.29340888211215915No Hit
ACTCTGCGTTGATACCACTGCTTCC121070.2647020369397847No Hit
GGTATCAACGCAGAGTACATGGGAA117840.2576401093002745No Hit
GCTTCCCATGTACTCTGCGTTGATA108460.23713209652671224No Hit
GGTATCAACGCAGAGTACTTTTTTT106210.23221279708742493No Hit
GTACTGGTTCACTATCGGTCAGTCA101640.2222211533374058No Hit
TATCAACGCAGAGTACTTTTTTTTT97960.21417536581003807No Hit
GTGGTATCAACGCAGAGTACATGGG85190.18625560854794962No Hit
CAGTGGTATCAACGCAGAGTACATG79720.17429624502221555No Hit
ATACCACTGCTTCCCATGTACTCTG69100.1510771516687794No Hit
GTACTCTGCGTTGATACCACTGCTT63200.1381776553613149No Hit
GCAGTGGTATCAACGCAGAGTACAT61820.135160485038552No Hit
GGATACCACGTGTCCCGCCCTACTC61720.13494184950791702No Hit
CATGGGAAGCAGTGGTATCAACGCA59080.12916987149915324No Hit
GGGAAGCAGTGGTATCAACGCAGAG58310.12748637791326378No Hit
GTTAATGATAGTGTGTCGAAACACA56040.12252335136784948No Hit
CTGCTTCCCATGTACTCTGCGTTGA54360.11885027445318161No Hit
GTACATGGTAAGCAGTGGTATCAAC51780.11320947776279883No Hit
ACATGGGAAGCAGTGGTATCAACGC51640.11290338801990983No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51230.11200698234430635No Hit
TACCACTGCTTCCCATGTACTCTGC51110.11174461970754436No Hit
GCAGAGTACATGGGAAGCAGTGGTA50690.11082635047887741No Hit
CCATGTACTCTGCGTTGATACCACT48200.1053823257660661No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA47730.10435473877208165No Hit
GTTGATACCACTGCTTCCCATGTAC46650.10199347504122372No Hit
ATACAGGGTGACAGCCCCGTACACA45840.1002225272430803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG2650.011.11186319
CAGTGCG951.365828E-510.99698459
TGCGTTT1053.4554541E-610.86105812
AAATCGC3000.010.77090417
ACCGTCT803.7832622E-410.6845718
TGTCCCG11750.010.50695311
AATCGCC3000.010.45548618
CCGTACA12700.010.40172917
CGCCCTA12300.010.121757516
CCCGTAC13150.010.11793816
CCCCGTA13250.09.89801715
GTCCCGC13000.09.576529512
GTACTGG24700.09.5387691
ACACCGT1502.6617636E-79.4976046
GACCGTT900.00111706999.49757
CCGCCCT13500.09.43313415
TCCCGCC13350.09.32556213
GTCCTAG2650.09.3210551
AGGACCG2054.2564352E-109.2659555
CCCGCCC13750.09.26162214
Produced by FastQC (version 0.11.5)