##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294606_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4573822
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.888089873195764	31.0	30.0	33.0	25.0	34.0
2	29.907220482126327	31.0	30.0	34.0	25.0	34.0
3	29.852938308486863	31.0	30.0	34.0	25.0	34.0
4	33.267061988857456	35.0	33.0	37.0	26.0	37.0
5	33.815013133436324	35.0	35.0	37.0	28.0	37.0
6	33.871552063023	37.0	35.0	37.0	28.0	37.0
7	33.78760017333425	37.0	35.0	37.0	28.0	37.0
8	33.60565824380573	37.0	35.0	37.0	27.0	37.0
9	34.63227799420266	38.0	34.0	39.0	25.0	39.0
10	34.77160326746428	38.0	34.0	39.0	27.0	39.0
11	35.046467046596916	39.0	35.0	39.0	27.0	39.0
12	34.80799843107143	38.0	35.0	39.0	27.0	39.0
13	34.64435454637282	38.0	34.0	39.0	26.0	39.0
14	35.519597395788466	39.0	34.0	41.0	25.0	41.0
15	35.489363600070135	39.0	34.0	41.0	25.0	41.0
16	35.59615831136411	39.0	34.0	41.0	26.0	41.0
17	35.63822619244912	39.0	34.0	41.0	26.0	41.0
18	35.523497197748405	39.0	34.0	41.0	25.0	41.0
19	35.75365460221233	39.0	34.0	41.0	26.0	41.0
20	35.893332534584864	39.0	34.0	41.0	27.0	41.0
21	35.92715217164113	39.0	34.0	41.0	27.0	41.0
22	35.98158695288098	39.0	35.0	41.0	27.0	41.0
23	35.972177316913516	39.0	35.0	41.0	27.0	41.0
24	35.97369617794484	39.0	34.0	41.0	27.0	41.0
25	34.78078049386268	38.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.1379045109128008
1101	2	1.1359692000904396
1101	3	1.4015568642228544
1101	4	1.1891996526029942
1101	5	0.8891811046338134
1101	6	0.8388485523886047
1101	7	0.8087741521560758
1101	8	1.0409788803457616
1101	9	1.306481166614894
1101	10	1.284964272571365
1101	11	0.9337796289653468
1101	12	1.2496558578821322
1101	13	1.3183572015276042
1101	14	1.3833824224976539
1101	15	1.577309077151689
1101	16	1.4367204747973261
1101	17	1.4212107282949589
1101	18	1.502180216359676
1101	19	1.30447676042305
1101	20	1.2321177742571479
1101	21	1.1644977662269156
1101	22	1.0306667985744582
1101	23	1.1777444619370598
1101	24	1.2020601226617487
1101	25	1.2092864609906115
1102	1	0.9968640727837936
1102	2	0.9283231392297431
1102	3	1.1625100962600783
1102	4	1.0628248915742091
1102	5	0.8685165651069511
1102	6	0.7150117367905935
1102	7	0.6632410154134618
1102	8	0.725458895474631
1102	9	1.0708215865819142
1102	10	1.0410121070674734
1102	11	0.8497531614653866
1102	12	1.018582777286106
1102	13	1.0152610033159988
1102	14	1.265225900492247
1102	15	1.3375209026518107
1102	16	1.2223872809373262
1102	17	1.1692489575428553
1102	18	1.2823830593711278
1102	19	1.1097155192580246
1102	20	0.34111321971063546
1102	21	0.6485871874285039
1102	22	0.6824388493754583
1102	23	0.8013110976880071
1102	24	0.8874855271625393
1102	25	0.9474033015571734
1103	1	0.6439330486268204
1103	2	0.6573829513197538
1103	3	0.9088788579545444
1103	4	0.6978505876655277
1103	5	0.6039181061346568
1103	6	0.4250700141022392
1103	7	0.3647539915923943
1103	8	0.4943334361857481
1103	9	0.5869811612185103
1103	10	0.5712679146727098
1103	11	0.46831902142407955
1103	12	0.7309871899746199
1103	13	0.5427445320607092
1103	14	0.5871265400302477
1103	15	0.7421566309381049
1103	16	0.7571450851865791
1103	17	0.7465708224602281
1103	18	0.817865207184191
1103	19	0.6890791352244889
1103	20	-0.205925686584564
1103	21	0.20121586697445792
1103	22	-0.31756039115553847
1103	23	0.15635160125501812
1103	24	0.32564242716629366
1103	25	0.5196324656877565
1104	1	0.3360494382136494
1104	2	0.3259417080326834
1104	3	0.616634016241921
1104	4	0.4764860731506104
1104	5	0.2802520361730032
1104	6	0.20137140632473916
1104	7	0.19754010896935625
1104	8	0.3677582018519274
1104	9	0.6206516863991993
1104	10	0.5097809284611472
1104	11	0.31007100620408323
1104	12	0.44064566331957167
1104	13	0.3243127290215284
1104	14	0.6513096160847169
1104	15	0.6382581537399972
1104	16	0.5410246042905058
1104	17	0.35629855907517083
1104	18	0.5530052589343057
1104	19	-0.17573168520475946
1104	20	0.17318396531967295
1104	21	-0.4517775184300774
1104	22	-0.0928512915379116
1104	23	-0.418596702149415
1104	24	-0.7677928142851727
1104	25	-1.9213074249787567
1105	1	0.10407645251110864
1105	2	0.2097560406182808
1105	3	0.5975795166530453
1105	4	0.4387809446384452
1105	5	0.3413675334707733
1105	6	0.2762150203651501
1105	7	0.26524254988888174
1105	8	0.3711470432866264
1105	9	0.6847780192189603
1105	10	-1.3166340624515982
1105	11	-0.33424697059746933
1105	12	0.30721688053293406
1105	13	0.3533635697590469
1105	14	0.7846335295389082
1105	15	-0.22474190480162548
1105	16	0.28443985597956356
1105	17	0.08960459072489613
1105	18	-0.4237300211740518
1105	19	-1.651586752115172
1105	20	-0.2729744935455187
1105	21	-1.4988609083092967
1105	22	-0.6510602638397387
1105	23	-0.17437690230853775
1105	24	-0.4290065284583662
1105	25	-0.5507686690946585
1106	1	-0.4034854891201647
1106	2	-0.3824520739589268
1106	3	0.08796272723096621
1106	4	0.033293797189244856
1106	5	-0.23138394551809682
1106	6	-0.5037446349844714
1106	7	-0.3671128408944213
1106	8	-0.22134927332420773
1106	9	-0.004514142533736276
1106	10	-0.4803151304975657
1106	11	-0.6433009483306762
1106	12	-0.4318454949156916
1106	13	-0.5070677508119914
1106	14	-0.7502831341597229
1106	15	-0.8579875236824819
1106	16	-0.43628895111599775
1106	17	-0.3356372070258402
1106	18	-0.49001009855127364
1106	19	-0.49313990061639146
1106	20	-0.5817035928408743
1106	21	-0.5790026070367773
1106	22	-0.693428930815081
1106	23	-1.0832859491872213
1106	24	-0.6120792988055612
1106	25	-0.8042990834344508
1107	1	0.35087791905510457
1107	2	0.43179957692436943
1107	3	0.6955812067037463
1107	4	0.4902700934795732
1107	5	0.325641124178496
1107	6	0.20332908378704673
1107	7	0.14782444401237171
1107	8	0.329060155679997
1107	9	0.4505805766290152
1107	10	-1.1620967179979331
1107	11	-0.24671528214680905
1107	12	0.1993437485056404
1107	13	0.30890171507007125
1107	14	0.4161769550083392
1107	15	0.5888872957410598
1107	16	0.47036595459585584
1107	17	0.0985071494683254
1107	18	-0.25664240437888
1107	19	0.13999657871808324
1107	20	0.18240093855077788
1107	21	0.3065591917937027
1107	22	0.3261157181063865
1107	23	0.4462811966010918
1107	24	0.2930643003331639
1107	25	0.21738708391126238
1108	1	-0.023003191541537404
1108	2	0.1609371038836258
1108	3	0.499046038493276
1108	4	-0.04994142160835935
1108	5	0.0929791747280504
1108	6	0.10858343524969882
1108	7	0.07617412371674703
1108	8	0.12403031059070457
1108	9	0.42990354265734254
1108	10	0.3745029052357012
1108	11	0.09149124020394339
1108	12	-0.05913894592453772
1108	13	0.12751690875467148
1108	14	0.41158426079520183
1108	15	0.07568836478648677
1108	16	0.20596488510830113
1108	17	0.19461578269949342
1108	18	0.36019925627897464
1108	19	0.1825621551175729
1108	20	0.07871912144685211
1108	21	0.04950444350852479
1108	22	-0.10700061484029533
1108	23	0.13448373987456108
1108	24	0.19461660269159609
1108	25	-0.04231478070846606
1109	1	-0.10295309023014454
1109	2	-0.1378607480694356
1109	3	0.29671682573184555
1109	4	0.14662656745769453
1109	5	0.15875493415367004
1109	6	-0.008066293721242346
1109	7	-0.12208248732351734
1109	8	-0.05102595314841807
1109	9	0.16416942816306346
1109	10	0.1724976331572492
1109	11	-0.03858075777927894
1109	12	0.07966308187846494
1109	13	0.18513593510615323
1109	14	0.39285801550917654
1109	15	-0.4301813781735788
1109	16	-0.013327428066418179
1109	17	0.15637688235874236
1109	18	0.1822672875750868
1109	19	0.23000826884037906
1109	20	0.3005696796921171
1109	21	0.19124971226732868
1109	22	0.17551097793291603
1109	23	0.10106785487126047
1109	24	0.25402912869591177
1109	25	0.11546709490290397
1110	1	0.020602917862795778
1110	2	0.06316669549392984
1110	3	0.14108334560478752
1110	4	-0.051942551956365435
1110	5	-0.15850458288225866
1110	6	-0.045293020194854705
1110	7	-0.1417864895769796
1110	8	-4.5053571484032773E-4
1110	9	0.29002961521217685
1110	10	0.3419440994500249
1110	11	-0.1110544856213096
1110	12	0.16719580923259514
1110	13	0.12586988504997976
1110	14	0.10874210958359498
1110	15	0.168914257506259
1110	16	-0.2767341782171471
1110	17	0.08745720618082942
1110	18	-5.134478068246903
1110	19	-1.9799690723766616
1110	20	-0.84672615291705
1110	21	-0.45106826358797036
1110	22	-0.48979478189672676
1110	23	-0.3924216760373511
1110	24	-0.08498075655558779
1110	25	-0.13790638513117415
1111	1	-0.6935448441742871
1111	2	-0.4661929452999978
1111	3	-1.6692429637656474
1111	4	-1.0355015586063558
1111	5	-0.716166601371917
1111	6	-0.7551138851941062
1111	7	-0.9106855588265574
1111	8	-2.39694063669403
1111	9	-0.6966934618922238
1111	10	-0.3605516475235291
1111	11	-0.6638942523774389
1111	12	-0.3266695658829377
1111	13	-1.1243783339111033
1111	14	-0.5408612013015173
1111	15	-0.40462397776521897
1111	16	-2.5298961673779345
1111	17	-2.3683034637191227
1111	18	-0.9994150288946457
1111	19	-0.6487072313998965
1111	20	-0.5280713648618942
1111	21	-0.4967957901643203
1111	22	-0.45287219695865133
1111	23	-0.621499793765814
1111	24	-0.5810078020635672
1111	25	-0.6121684405050303
1112	1	-0.4787725368098954
1112	2	-0.5386203161009568
1112	3	-0.11617105529680671
1112	4	-0.6631165430947661
1112	5	-2.0874266573814815
1112	6	-1.0756711779557335
1112	7	-0.846134064680534
1112	8	-0.3606702856677728
1112	9	-0.608250362250736
1112	10	-0.45725774829344346
1112	11	-1.0419723831887993
1112	12	-1.6746538191182339
1112	13	-1.771515210176105
1112	14	-2.9659505564313235
1112	15	-4.120193352595376
1112	16	-1.5552188645213434
1112	17	-0.9055552430814018
1112	18	-0.47737737910723865
1112	19	-0.3473676560064902
1112	20	-0.43449619399744677
1112	21	-0.4375228640170974
1112	22	-0.45715430554756864
1112	23	-0.360526794432559
1112	24	-0.4193148643233471
1112	25	-0.36225375798241544
1113	1	-0.8659443061709062
1113	2	-0.5823975615815193
1113	3	-0.9273519842139706
1113	4	-3.0549443603480313
1113	5	-1.1437187991545414
1113	6	-0.9075865629267099
1113	7	-0.9281747631179584
1113	8	-1.4132305237837954
1113	9	-2.7333631011675905
1113	10	-1.2432687741503727
1113	11	-1.3250885292228602
1113	12	-2.8485327799518068
1113	13	-2.0249759904259506
1113	14	-1.5849092850231372
1113	15	-0.8979153616479891
1113	16	-1.1514584269805255
1113	17	-0.8144287325942727
1113	18	-0.6000649040673309
1113	19	-0.47033410582871227
1113	20	-0.5380362400205456
1113	21	-0.5573824903362095
1113	22	-0.5553878002527668
1113	23	-0.38437179608939687
1113	24	-0.4950674072902572
1113	25	-0.641179085380152
1201	1	1.136718432129907
1201	2	1.1395982185600317
1201	3	1.3584146832678847
1201	4	1.1452714553255703
1201	5	0.7998456985321525
1201	6	0.8071305100977213
1201	7	0.842717526937605
1201	8	0.9247180510997381
1201	9	1.3082335023651055
1201	10	1.215625054756842
1201	11	1.0033993448468692
1201	12	1.312196166028265
1201	13	1.2003318409632442
1201	14	1.5268754771216067
1201	15	1.5716292902058768
1201	16	1.4872711046984222
1201	17	1.3760684741665798
1201	18	1.5311846251771257
1201	19	1.2392228188034764
1201	20	1.098834562317542
1201	21	1.1046096354559936
1201	22	1.0827284832798014
1201	23	1.0703854409111813
1201	24	1.166905082317136
1201	25	1.2068504560387368
1202	1	1.0310145334663332
1202	2	1.003717103100204
1202	3	1.2098620208290143
1202	4	1.2108270579660285
1202	5	0.8657230266002927
1202	6	0.7797955417036491
1202	7	0.844926848147523
1202	8	0.9661901450500423
1202	9	1.314239555542315
1202	10	1.113933292088639
1202	11	1.054122654385921
1202	12	1.1679118421782704
1202	13	1.2471415452964933
1202	14	1.4189937437100255
1202	15	1.525139634839924
1202	16	1.3401502098709273
1202	17	1.4610850911292488
1202	18	1.477369260550084
1202	19	1.3140675616660076
1202	20	1.2265130106782252
1202	21	0.9551848332416881
1202	22	1.031024599380352
1202	23	0.9686368899082964
1202	24	1.0605103270065328
1202	25	1.0266365430742468
1203	1	0.7288946779258154
1203	2	0.6083923919160057
1203	3	0.8978552969749849
1203	4	0.75081735212202
1203	5	0.5588014450788847
1203	6	0.5391184306825778
1203	7	0.5267527320663348
1203	8	0.6186933287512488
1203	9	0.9523428839312729
1203	10	0.654733558900574
1203	11	0.5362703873404442
1203	12	0.8393244660967909
1203	13	0.8329214961219691
1203	14	0.9172303068667134
1203	15	0.9902249352052834
1203	16	1.025146515855667
1203	17	0.9715621678220998
1203	18	1.0241083208435313
1203	19	0.709706582407371
1203	20	-0.11385246856264075
1203	21	0.3633728605936426
1203	22	0.47966577024490675
1203	23	0.5163584968027379
1203	24	0.65486415747867
1203	25	0.6654318682902556
1204	1	0.32640667766520437
1204	2	0.36465529693485266
1204	3	0.6411577391638446
1204	4	0.4842659204914668
1204	5	0.2869620704007332
1204	6	0.33890383873196583
1204	7	0.28851435356772726
1204	8	0.38274476523585577
1204	9	-0.672411582275835
1204	10	-0.3248066400376146
1204	11	0.11700443333392485
1204	12	0.3463467056066065
1204	13	0.4859255892687244
1204	14	-1.5520724408034
1204	15	-0.03950156199263688
1204	16	0.3267372512305968
1204	17	0.34897875428914915
1204	18	0.5896652772406128
1204	19	0.5175122216862178
1204	20	-0.6002776453955079
1204	21	-0.5457904767372668
1204	22	-1.5504062271127665
1204	23	-1.100938483830049
1204	24	-0.056432112090412545
1204	25	-2.8005786501023664
1205	1	-0.2772656413160739
1205	2	-0.31071878429049704
1205	3	0.11413687579968013
1205	4	0.035224724149834685
1205	5	0.13482661474640878
1205	6	-0.005388095567617768
1205	7	0.05939106476375855
1205	8	0.26979152587961863
1205	9	0.5861009628883522
1205	10	0.46992935400355407
1205	11	0.2864721492898852
1205	12	0.39024815379077893
1205	13	0.48049296980416756
1205	14	0.5925501128518107
1205	15	0.05571186878560752
1205	16	-0.7185914156675821
1205	17	-0.9551792784724569
1205	18	-0.6081870518129122
1205	19	-0.6132501152346634
1205	20	-0.04854671645318831
1205	21	-0.3552516775123138
1205	22	-0.005647840161451256
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2111	22	-0.40251645661783897
2111	23	-0.37152606523842735
2111	24	-0.4318505713887717
2111	25	-0.3076062752464068
2112	1	-0.9065389755927988
2112	2	-0.31387322975682963
2112	3	-0.15280402939945859
2112	4	0.04194503414032624
2112	5	-0.5750107313366826
2112	6	-0.6851021841822273
2112	7	-0.26476918639974656
2112	8	0.02477814952472812
2112	9	-2.3454663813140826
2112	10	-0.7764045693975064
2112	11	-1.0840346247073072
2112	12	-0.6344694427628781
2112	13	-0.21919734543053693
2112	14	-0.5112463324014769
2112	15	-0.7361100452149429
2112	16	-0.9465159923638993
2112	17	-0.8854787442203289
2112	18	-0.22191535883879965
2112	19	-0.12413355898612366
2112	20	0.022808697143631207
2112	21	-0.07373480725810566
2112	22	-0.009984458308188948
2112	23	-0.14252599440472835
2112	24	-0.012498718235697481
2112	25	0.15208430780298698
2113	1	0.0023698428308023267
2113	2	-0.38984084207720926
2113	3	-0.1688921223559383
2113	4	-0.07211434292536723
2113	5	-0.15078693769125806
2113	6	-0.6268268737095752
2113	7	-0.30550077143369947
2113	8	0.05443165893073143
2113	9	-0.9276535426262242
2113	10	-0.12457379058963625
2113	11	-0.34765335692952704
2113	12	-0.537927354422834
2113	13	-0.038777447161919554
2113	14	0.18996666517727334
2113	15	-2.1826325818362946
2113	16	-0.6114440288207561
2113	17	-1.700815597383432
2113	18	-0.2004742478140784
2113	19	-0.06529152448745634
2113	20	0.04840937517246857
2113	21	0.01726447031187206
2113	22	0.1776199810196033
2113	23	0.337016152949559
2113	24	0.27711058149220236
2113	25	0.3641251169287756
2114	1	-0.6809145944578674
2114	2	-0.5598545407930828
2114	3	-3.0775214919020755
2114	4	-1.31725865275574
2114	5	-1.1062346611696512
2114	6	-1.5218244954059585
2114	7	-3.4722914384696253
2114	8	-1.4828454728977007
2114	9	-0.8256261170303603
2114	10	-0.7394450336131442
2114	11	-0.7257817649838358
2114	12	-0.6043280657233723
2114	13	-2.971826714034183
2114	14	-2.423290030451497
2114	15	-1.4635078914460493
2114	16	-1.836199431246932
2114	17	-1.6149776521751704
2114	18	-0.6568322591767384
2114	19	-0.5685859052757749
2114	20	-0.21327677950176138
2114	21	-0.22313930931326098
2114	22	-0.3564605229930038
2114	23	-0.20487625697610667
2114	24	-0.330805296970226
2114	25	-0.16668580636936525
2201	1	1.2657847746117348
2201	2	1.2336686820689877
2201	3	1.4026458254359149
2201	4	1.2235679855463317
2201	5	0.9884868489073142
2201	6	0.9299512139037063
2201	7	0.8419373536714048
2201	8	1.0773606509454439
2201	9	1.4405070251276868
2201	10	1.3685776398797245
2201	11	1.180909694727788
2201	12	1.2797328895816094
2201	13	1.3880421906301308
2201	14	1.6929150621879927
2201	15	1.7885506196205583
2201	16	1.626052670772097
2201	17	1.627377089673253
2201	18	1.713981495573421
2201	19	1.4894071568276246
2201	20	1.3735053715838603
2201	21	1.3334792258580563
2201	22	1.3134629419551658
2201	23	1.2019817668013246
2201	24	1.2852247897599796
2201	25	1.5080433403290954
2202	1	1.1813360327839995
2202	2	1.1072412491466643
2202	3	1.3598529795143044
2202	4	1.2004432036235215
2202	5	0.9981361398390689
2202	6	0.9596327270194607
2202	7	0.9560165806010943
2202	8	1.0385158825864167
2202	9	1.389096260264452
2202	10	1.3107537266617513
2202	11	1.0377648947526907
2202	12	1.3083179966093894
2202	13	1.3047189318484769
2202	14	-0.8675511684062087
2202	15	0.7267270274777289
2202	16	1.216937740614128
2202	17	1.409055657416019
2202	18	1.526331831146095
2202	19	1.4446617317388757
2202	20	1.293376155220166
2202	21	1.2522824346437176
2202	22	1.194742786790691
2202	23	1.2142701807392555
2202	24	1.248497806834294
2202	25	1.4336562088897722
2203	1	0.9210980798460433
2203	2	0.947948440182568
2203	3	1.239310802430591
2203	4	0.9547706863882581
2203	5	0.7158199814549491
2203	6	0.6504085609321564
2203	7	0.677228923579662
2203	8	0.8344909570229433
2203	9	1.033805473317571
2203	10	0.9093221872588373
2203	11	0.8234480099602379
2203	12	1.049611057648022
2203	13	1.0716356401887595
2203	14	0.9149823703814164
2203	15	1.1638398525149611
2203	16	1.0870066003292322
2203	17	1.1313188530434743
2203	18	1.3151287444527853
2203	19	1.0581192592129653
2203	20	0.29495893934272743
2203	21	0.7202634125031722
2203	22	-0.19630802520101298
2203	23	0.15322504720074193
2203	24	0.4893161275029172
2203	25	0.8996060894254185
2204	1	0.7601642312460122
2204	2	0.6104989151257314
2204	3	0.9077732592286942
2204	4	0.7844880901804032
2204	5	0.592827289412142
2204	6	0.5809012450407351
2204	7	0.5172104473966428
2204	8	0.6327918146605924
2204	9	0.9693495395165783
2204	10	-1.5732687840076025
2204	11	-0.04602114227230203
2204	12	0.47592155484935006
2204	13	0.6780816223462551
2204	14	0.9471902768971177
2204	15	1.0050866748547946
2204	16	0.8164252538159005
2204	17	0.7300091492653991
2204	18	1.0256185405006875
2204	19	0.9110918194628823
2204	20	-0.1480472197375846
2204	21	0.4226933770318624
2204	22	0.5534445325311097
2204	23	0.6065399960355222
2204	24	0.7712596389403785
2204	25	0.11148723250502002
2205	1	0.4534166995967688
2205	2	0.2821046810717256
2205	3	0.6117357122563973
2205	4	0.418396870671522
2205	5	0.25560792649329755
2205	6	0.2036108305545099
2205	7	0.3716442844010075
2205	8	0.4747886715775991
2205	9	0.6060359960619408
2205	10	0.5406722381953912
2205	11	0.41108648634796197
2205	12	0.4324279668328259
2205	13	0.3889191901399087
2205	14	0.758639877446214
2205	15	0.2770209395339833
2205	16	0.3119263239184775
2205	17	0.5879686485902766
2205	18	-0.02293079172495993
2205	19	-0.6007023928280972
2205	20	0.02539852886076943
2205	21	-0.8143154704747246
2205	22	-1.5265734271957854
2205	23	-1.7271504087201208
2205	24	-0.941129185223275
2205	25	-1.6118442994497428
2206	1	-0.11707422234745835
2206	2	-0.3834839293199188
2206	3	0.29328507682440375
2206	4	0.06959589635873442
2206	5	-0.18436472727388775
2206	6	-0.14839352742284007
2206	7	-0.13549107757428658
2206	8	-0.04206834374102186
2206	9	0.4283484046215662
2206	10	-0.2445916563301438
2206	11	-0.1524385046323644
2206	12	0.11671233011123405
2206	13	0.16656173012589193
2206	14	-0.011585015659825615
2206	15	-0.12920126700365842
2206	16	-0.6930057591368808
2206	17	-0.46338768175851897
2206	18	0.0901422768534843
2206	19	-0.5780409856684443
2206	20	-0.37990905806694997
2206	21	-2.4828437633512266
2206	22	-1.3702785188736186
2206	23	-2.1151704010538595
2206	24	-1.2120355942056378
2206	25	-1.8111837700425895
2207	1	0.6029671424539309
2207	2	0.47727517462074687
2207	3	0.8390433924832799
2207	4	0.5096386692141976
2207	5	0.4314757953226689
2207	6	0.43768818655311037
2207	7	0.42997271201616627
2207	8	0.3994075454037116
2207	9	-1.125936920827428
2207	10	-0.0770077885741216
2207	11	0.20436729472250192
2207	12	0.5992954724195201
2207	13	0.6553909917172049
2207	14	0.90653015687964
2207	15	0.9122445617575465
2207	16	0.9209927117106815
2207	17	0.8663170038912611
2207	18	0.8767029005786
2207	19	-0.25485584740781775
2207	20	0.33317159287101816
2207	21	-0.2935683228416579
2207	22	0.2738674035103301
2207	23	-0.4946721184228551
2207	24	-1.5246149293413538
2207	25	-0.25694854047265636
2208	1	0.6088947984066806
2208	2	0.2576120960656212
2208	3	0.7771784466040188
2208	4	0.6498991360528521
2208	5	0.5810860265551838
2208	6	0.3968617106597563
2208	7	0.4727659138880611
2208	8	0.6961968224432695
2208	9	0.030189672159423253
2208	10	0.24983791507360564
2208	11	0.4059548145404861
2208	12	0.4383245812117025
2208	13	0.7443980276134923
2208	14	0.9748930823388733
2208	15	0.8898573116336834
2208	16	0.7555090205091162
2208	17	0.6679086716040317
2208	18	0.8529708943938061
2208	19	-1.216808365775229
2208	20	0.08424404511593764
2208	21	-0.04661907326296699
2208	22	0.3694274226430281
2208	23	0.5529535475499898
2208	24	0.7028308808658394
2208	25	0.7972388474127499
2209	1	0.19449148138314243
2209	2	-1.3836552268043008
2209	3	-0.20881645864137965
2209	4	0.16255307092279736
2209	5	0.13835561591739776
2209	6	0.2217941082778836
2209	7	0.14931083175730464
2209	8	0.3289658408620113
2209	9	-1.8326376881949784
2209	10	-0.29807359892738106
2209	11	0.0665731734831283
2209	12	0.32772811425510895
2209	13	0.4575441208438775
2209	14	0.6847172516359521
2209	15	0.7552142468834688
2209	16	0.6618858559825824
2209	17	0.4922892893782418
2209	18	0.6314349446041589
2209	19	0.45276672136955654
2209	20	0.370290297987971
2209	21	0.4316023363299948
2209	22	0.3435822020027004
2209	23	0.4242047713173349
2209	24	0.4553411796870108
2209	25	0.4898872119292861
2210	1	0.1343150907446713
2210	2	0.21001787079786993
2210	3	0.16268716256953297
2210	4	0.4059976923046449
2210	5	0.17614875452910894
2210	6	0.14546960781405005
2210	7	0.15638790271754743
2210	8	0.24796641402686959
2210	9	0.4239635763712073
2210	10	-1.412589353005373
2210	11	-0.45411232186574324
2210	12	-2.2911543862459283
2210	13	-0.6690237026704793
2210	14	-0.4343850147110331
2210	15	-0.6143965731213541
2210	16	-0.03201929207681076
2210	17	0.14577654538099694
2210	18	0.3267057436502654
2210	19	0.2861559321347542
2210	20	0.3181988195186207
2210	21	0.26578383432485
2210	22	0.26617402332970386
2210	23	0.16071095065230168
2210	24	0.20005765612354764
2210	25	0.12563460007468308
2211	1	-0.3478134867880449
2211	2	-0.11919009829406946
2211	3	-1.4517019584623156
2211	4	-0.6700233520026586
2211	5	-0.4284098824216045
2211	6	-0.4016729111462993
2211	7	-0.36470437836805303
2211	8	-0.09633644748529946
2211	9	0.11550688427177391
2211	10	0.09454715114726042
2211	11	-0.24576353269441853
2211	12	-0.25569769443205814
2211	13	-0.31173531962574685
2211	14	-0.84494685143666
2211	15	-0.27104504877832625
2211	16	-0.1095631231200187
2211	17	-0.164839775850659
2211	18	0.0059207184369043375
2211	19	-0.06207114165795957
2211	20	-0.009772182806869978
2211	21	-0.15332116628481174
2211	22	-0.172465395563151
2211	23	-0.478225848558111
2211	24	-0.34277724106931373
2211	25	-0.16080831460271128
2212	1	-0.513729522528358
2212	2	-0.36160040652674397
2212	3	-2.4405135938822085
2212	4	-1.417742898926324
2212	5	-0.6249364905961841
2212	6	-0.4279974420302324
2212	7	-0.3180006812790168
2212	8	-0.3707482297005953
2212	9	-0.951015624482821
2212	10	-0.23348689061708683
2212	11	-0.2513082996416358
2212	12	0.03241719030567225
2212	13	-0.32832335127869783
2212	14	-0.14826790911715193
2212	15	-0.020380513207186368
2212	16	-1.2047382037708303
2212	17	-1.314187146683139
2212	18	-0.30010463768136475
2212	19	-0.178228696534795
2212	20	-0.277413328323469
2212	21	-0.041787515999999414
2212	22	-0.4091798837724241
2212	23	-0.11379920594539072
2212	24	-0.22457216155638804
2212	25	-0.006872532318084268
2213	1	-3.3158722768538382
2213	2	-1.517159387095468
2213	3	-2.625599380749623
2213	4	-1.177620972923087
2213	5	-0.8842208428028329
2213	6	-0.6937470446762362
2213	7	-0.4298422356151903
2213	8	-0.19800462736954216
2213	9	-0.4440631938297983
2213	10	-0.11272891957434439
2213	11	-0.5642856678289263
2213	12	-0.2695523820470527
2213	13	-0.7097482634803569
2213	14	-0.40029675719480196
2213	15	-0.4253141531305502
2213	16	-1.691486597860525
2213	17	-1.0282532269759415
2213	18	-0.4236050931255022
2213	19	-0.34034154473489053
2213	20	-0.34617622361525235
2213	21	-0.1503033334319781
2213	22	-0.20980857686711119
2213	23	-0.16997127949993995
2213	24	-0.10095510383873574
2213	25	-0.0778875635487779
2214	1	-0.9623389076472897
2214	2	-0.7337188747402905
2214	3	-6.453991307676727
2214	4	-2.2316025674969318
2214	5	-1.2643669034535137
2214	6	-0.783190490608888
2214	7	-0.5240944546771544
2214	8	-0.408508076607518
2214	9	-0.17865561460334334
2214	10	-0.07437744423759085
2214	11	-1.0022022177313943
2214	12	-0.6211945577208624
2214	13	-5.135879988131887
2214	14	-3.8549437551450367
2214	15	-2.3106350265551825
2214	16	-2.9395880764849878
2214	17	-3.4344021615509774
2214	18	-1.2583152067823136
2214	19	-0.6703431973973082
2214	20	-0.31884530867341
2214	21	-0.43818617254603254
2214	22	-0.4168360933574675
2214	23	-0.3267985038798429
2214	24	-0.20023518565027132
2214	25	-0.2153210452421206
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1695.0
3	254.0
4	216.0
5	476.0
6	75.0
7	159.0
8	345.0
9	831.0
10	1828.0
11	3906.0
12	8052.0
13	13855.0
14	21644.0
15	30609.0
16	37945.0
17	41037.0
18	41382.0
19	40553.0
20	39903.0
21	39478.0
22	38865.0
23	38844.0
24	40512.0
25	42990.0
26	47227.0
27	53221.0
28	61028.0
29	74733.0
30	91511.0
31	114105.0
32	148390.0
33	196943.0
34	263683.0
35	360189.0
36	503306.0
37	754735.0
38	1419297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.50249130227254	20.284083087617198	12.569001314644243	22.64442429546602
2	14.44900439532988	22.09483963816239	39.746137051254934	23.7100189152528
3	19.48278573892705	29.237260234896134	28.06796408109121	23.211989945085605
4	12.454325673696314	18.913021032939763	36.4098535796597	32.22279971370423
5	12.366788110412314	40.18858341295423	33.86937447721443	13.57525399941903
6	30.049114402712725	37.14340972403477	18.580685868883425	14.22679000436908
7	25.34684362240031	34.63570699742897	22.53100896621611	17.486440413954604
8	25.31116156337055	36.549030451764544	21.419798919209345	16.72000906565556
9	26.196419656229942	17.86874902370371	20.982439574643294	34.95239174542306
10	16.115132026580778	31.23022511049435	31.38698028494656	21.267662577978317
11	30.825814956598286	28.06028614787625	21.408652953217505	19.705245942307958
12	22.01218965279616	29.55743597260172	29.887707308322526	18.5426670662796
13	27.53643670048947	24.386654526569007	25.39551939389352	22.681389379048007
14	20.923677567371087	25.67081215903167	27.22027990003914	26.185230373558106
15	25.941954252202493	28.97340818750502	23.479060645924612	21.605576914367873
16	20.466779010870756	31.102798447016028	27.224101310174355	21.20632123193886
17	21.727293848839036	30.292924303130516	27.561430444752965	20.418351403277487
18	22.208302426131425	28.316565355267155	28.951275945629014	20.523856272972406
19	21.670875009271043	29.913844386057836	29.351958996062518	19.063321608608604
20	23.259219653659727	27.582391233770498	28.159945884879356	20.99844322769042
21	22.170792775035302	29.012304491593373	28.60119949377556	20.215703239595765
22	22.134290241752538	28.56091157165951	26.607634366704875	22.69716381988308
23	20.89852975376444	29.128281352312374	28.217163177139042	21.756025716784148
24	21.61139469085335	30.118509808572185	27.043486270930888	21.22660922964358
25	22.09721906505505	27.55440105943713	26.604224402715626	23.744155472792194
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8068.0
1	8068.0
2	8409.5
3	8751.0
4	8751.0
5	8751.0
6	12715.5
7	16680.0
8	16680.0
9	16680.0
10	20685.5
11	24691.0
12	24691.0
13	24691.0
14	33753.5
15	42816.0
16	42816.0
17	42816.0
18	64604.5
19	86393.0
20	86393.0
21	86393.0
22	121406.0
23	156419.0
24	156419.0
25	156419.0
26	204535.0
27	252651.0
28	252651.0
29	252651.0
30	298831.0
31	345011.0
32	345011.0
33	345011.0
34	383404.0
35	421797.0
36	421797.0
37	421797.0
38	434451.5
39	447106.0
40	447106.0
41	447106.0
42	467898.5
43	488691.0
44	488691.0
45	488691.0
46	612925.0
47	737159.0
48	737159.0
49	737159.0
50	661255.5
51	585352.0
52	585352.0
53	585352.0
54	476195.0
55	367038.0
56	367038.0
57	367038.0
58	303925.5
59	240813.0
60	240813.0
61	240813.0
62	207615.5
63	174418.0
64	174418.0
65	174418.0
66	133374.5
67	92331.0
68	92331.0
69	92331.0
70	69201.5
71	46072.0
72	46072.0
73	46072.0
74	33049.0
75	20026.0
76	20026.0
77	20026.0
78	13747.0
79	7468.0
80	7468.0
81	7468.0
82	5120.0
83	2772.0
84	2772.0
85	2772.0
86	1788.5
87	805.0
88	805.0
89	805.0
90	574.5
91	344.0
92	344.0
93	344.0
94	218.5
95	93.0
96	93.0
97	93.0
98	75.0
99	57.0
100	57.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09893257761233384
2	0.05201339273806458
3	0.055205471485335465
4	0.050854624425699116
5	0.047181547511031256
6	0.06701179013962502
7	0.06309821414125867
8	0.0595563185449718
9	0.0650659339169736
10	0.06559065919049757
11	0.05879109418774933
12	0.059731226969479786
13	0.059993589606241786
14	0.06193944582889321
15	0.0651315245761641
16	0.060146634477686284
17	0.06296703282287767
18	0.13962064986350584
19	0.06862969306632397
20	0.06178640095744871
21	0.0596219092041623
22	0.06539388721292608
23	0.06460679930264011
24	0.07615075532016768
25	0.067623969625403
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4573822.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.213246261214174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.81578584869168	40.108015432664295
2	13.649579533367945	14.25377715075539
3	4.562487032975617	7.14666777049057
4	1.9662651491177137	4.106603457829048
5	0.9584604249579844	2.5022165099979614
6	0.5381980252224101	1.6860639616942115
7	0.34027810995117913	1.2436917326527002
8	0.22414228243906706	0.9362556952433303
9	0.15602451289027666	0.7331891682893402
>10	0.708074590829437	6.356412097919096
>50	0.04106242276563069	1.47111343360805
>100	0.028536753041798035	3.1796925282638875
>500	0.005210955111087315	1.8495924289782788
>1k	0.004612976325626535	5.3538377291583314
>5k	6.834039000928155E-4	2.2367500126880047
>10k+	5.979784125812135E-4	6.836120889767599
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	61682	1.3485876800627572	No Hit
CCCATGTACTCTGCGTTGATACCAC	58602	1.2812479366271796	No Hit
GAGTACATGGGAAGCAGTGGTATCA	27915	0.61032108376758	No Hit
CATGTACTCTGCGTTGATACCACTG	21650	0.4733459238247575	No Hit
GCGTTGATACCACTGCTTCCCATGT	19003	0.4154730988656752	No Hit
GTATCAACGCAGAGTACATGGGAAG	17199	0.3760312491391226	No Hit
GTATCAACGCAGAGTACTTTTTTTT	16758	0.3663894222381195	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	14778	0.32309958717239107	No Hit
TATCAACGCAGAGTACATGGGAAGC	13420	0.29340888211215915	No Hit
ACTCTGCGTTGATACCACTGCTTCC	12107	0.2647020369397847	No Hit
GGTATCAACGCAGAGTACATGGGAA	11784	0.2576401093002745	No Hit
GCTTCCCATGTACTCTGCGTTGATA	10846	0.23713209652671224	No Hit
GGTATCAACGCAGAGTACTTTTTTT	10621	0.23221279708742493	No Hit
GTACTGGTTCACTATCGGTCAGTCA	10164	0.2222211533374058	No Hit
TATCAACGCAGAGTACTTTTTTTTT	9796	0.21417536581003807	No Hit
GTGGTATCAACGCAGAGTACATGGG	8519	0.18625560854794962	No Hit
CAGTGGTATCAACGCAGAGTACATG	7972	0.17429624502221555	No Hit
ATACCACTGCTTCCCATGTACTCTG	6910	0.1510771516687794	No Hit
GTACTCTGCGTTGATACCACTGCTT	6320	0.1381776553613149	No Hit
GCAGTGGTATCAACGCAGAGTACAT	6182	0.135160485038552	No Hit
GGATACCACGTGTCCCGCCCTACTC	6172	0.13494184950791702	No Hit
CATGGGAAGCAGTGGTATCAACGCA	5908	0.12916987149915324	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	5831	0.12748637791326378	No Hit
GTTAATGATAGTGTGTCGAAACACA	5604	0.12252335136784948	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	5436	0.11885027445318161	No Hit
GTACATGGTAAGCAGTGGTATCAAC	5178	0.11320947776279883	No Hit
ACATGGGAAGCAGTGGTATCAACGC	5164	0.11290338801990983	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5123	0.11200698234430635	No Hit
TACCACTGCTTCCCATGTACTCTGC	5111	0.11174461970754436	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	5069	0.11082635047887741	No Hit
CCATGTACTCTGCGTTGATACCACT	4820	0.1053823257660661	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	4773	0.10435473877208165	No Hit
GTTGATACCACTGCTTCCCATGTAC	4665	0.10199347504122372	No Hit
ATACAGGGTGACAGCCCCGTACACA	4584	0.1002225272430803	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.372710612699838E-5	0.0
4	0.0	0.0	0.0	6.559065919049757E-5	0.0
5	2.186355306349919E-5	0.0	0.0	8.745421225399676E-5	0.0
6	2.186355306349919E-5	0.0	0.0	8.745421225399676E-5	0.0
7	2.186355306349919E-5	0.0	0.0	8.745421225399676E-5	0.0
8	6.559065919049757E-5	0.0	0.0	8.745421225399676E-5	0.0
9	6.559065919049757E-5	0.0	0.0	1.5304487144449433E-4	0.0
10	1.0931776531749596E-4	0.0	0.0	1.967719775714927E-4	0.0
11	1.0931776531749596E-4	0.0	0.0	1.967719775714927E-4	0.0
12	1.0931776531749596E-4	0.0	0.0	1.967719775714927E-4	0.0
13	1.0931776531749596E-4	0.0	0.0	2.1863553063499193E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGCCG	265	0.0	11.111863	19
CAGTGCG	95	1.365828E-5	10.9969845	9
TGCGTTT	105	3.4554541E-6	10.861058	12
AAATCGC	300	0.0	10.770904	17
ACCGTCT	80	3.7832622E-4	10.684571	8
TGTCCCG	1175	0.0	10.506953	11
AATCGCC	300	0.0	10.455486	18
CCGTACA	1270	0.0	10.401729	17
CGCCCTA	1230	0.0	10.1217575	16
CCCGTAC	1315	0.0	10.117938	16
CCCCGTA	1325	0.0	9.898017	15
GTCCCGC	1300	0.0	9.5765295	12
GTACTGG	2470	0.0	9.538769	1
ACACCGT	150	2.6617636E-7	9.497604	6
GACCGTT	90	0.0011170699	9.4975	7
CCGCCCT	1350	0.0	9.433134	15
TCCCGCC	1335	0.0	9.325562	13
GTCCTAG	265	0.0	9.321055	1
AGGACCG	205	4.2564352E-10	9.265955	5
CCCGCCC	1375	0.0	9.261622	14
>>END_MODULE