FastQCFastQC Report
Wed 25 May 2016
SRR1294606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294606_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4573822
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC664741.4533578263430453No Hit
GTACATGGGAAGCAGTGGTATCAAC597521.3063910226502038No Hit
GTATCAACGCAGAGTACTTTTTTTT401960.8788273789404135No Hit
GAGTACATGGGAAGCAGTGGTATCA290300.6346989454333816No Hit
GGTATCAACGCAGAGTACTTTTTTT275860.6031279748096887No Hit
CATGTACTCTGCGTTGATACCACTG247510.5411448018746685No Hit
TATCAACGCAGAGTACTTTTTTTTT243180.5316778833981733No Hit
GCGTTGATACCACTGCTTCCCATGT209200.4573855300884031No Hit
ACGCAGAGTACATGGGAAGCAGTGG175310.3832899487562043No Hit
GTATCAACGCAGAGTACATGGGAAG172220.3765341108595831No Hit
ACGCAGAGTACTTTTTTTTTTTTTT154580.33796680325557055No Hit
ACTCTGCGTTGATACCACTGCTTCC142180.3108559974568315No Hit
TATCAACGCAGAGTACATGGGAAGC141850.3101345002057361No Hit
GGTATCAACGCAGAGTACATGGGAA125720.27486858911431183No Hit
GCTTCCCATGTACTCTGCGTTGATA119870.2620784105721648No Hit
GTACTGGTTCACTATCGGTCAGTCA96590.21118005904033868No Hit
CAGTGGTATCAACGCAGAGTACATG94260.20608585117654338No Hit
GTACTTTTTTTTTTTTTTTTTTTTT89390.19543830083461927No Hit
GTGGTATCAACGCAGAGTACATGGG88580.19366735303647584No Hit
GAGTACTTTTTTTTTTTTTTTTTTT76990.16832749503588026No Hit
CATGGGAAGCAGTGGTATCAACGCA74980.16393292087011693No Hit
ATACCACTGCTTCCCATGTACTCTG72850.1592759840675916No Hit
GCAGTGGTATCAACGCAGAGTACAT70570.1542910939691138No Hit
GGGAAGCAGTGGTATCAACGCAGAG65700.14364354362718967No Hit
GGATACCACGTGTCCCGCCCTACTC64760.14158836963922078No Hit
ACATGGGAAGCAGTGGTATCAACGC64180.1403202835615378No Hit
GTACTCTGCGTTGATACCACTGCTT62730.13715006836733043No Hit
CTGCTTCCCATGTACTCTGCGTTGA60410.13207772405659862No Hit
CCATGTACTCTGCGTTGATACCACT57360.12540934037223136No Hit
TACCACTGCTTCCCATGTACTCTGC56730.12403193652923092No Hit
GCAGAGTACATGGGAAGCAGTGGTA55660.12169253635143651No Hit
GTTAATGATAGTGTGTCGAAACACA55330.12097103910034103No Hit
GATACCACTGCTTCCCATGTACTCT51570.11275034314846533No Hit
ATACAGGGTGACAGCCCCGTACACA50270.10990808125021043No Hit
CTTCCCATGTACTCTGCGTTGATAC50170.10968944571957545No Hit
AGCGTACACGGTGGATGCCCTGGCA49670.10859626806640048No Hit
AAGCAGTGGTATCAACGCAGAGTAC49280.107743589496924No Hit
GGTATCAACGCAGAGTACATGGGGG49070.10728445488259054No Hit
GTACATGGTAAGCAGTGGTATCAAC48930.10697836513970155No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA48720.10651923052536806No Hit
CCACTGCTTCCCATGTACTCTGCGT47850.10461710140884364No Hit
ATGGGAAGCAGTGGTATCAACGCAG47640.10415796679451016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCT400.00520853914.27977854
ACCGCGT655.3511612E-513.18133458
ATCGCCG2400.010.66897219
TGTCCCG14700.010.39422311
TGGTTCG1151.0023561E-59.933765
CTAGGAC3350.09.9243323
CGCCCTA15500.09.91171116
TCCCGCC15850.09.87190413
AATCGCC2700.09.83466418
CCGTACA15050.09.82999717
CCCGTAC15150.09.82770916
CCCCGTA15200.09.79495315
CCCGCCC16100.09.77751514
GTCCCGC16100.09.7187212
GTACTGG25200.09.5835231
CCCCGGT8950.09.21862719
CCGCCCT16700.09.19908915
CTACACT5200.09.1537054
CGAAACC1252.691049E-59.13865810
GCGCCTT10100.09.10744713
Produced by FastQC (version 0.11.5)