FastQCFastQC Report
Wed 25 May 2016
SRR1294605_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294605_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3572120
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC335510.9392461619430479No Hit
CCCATGTACTCTGCGTTGATACCAC308300.8630729090848012No Hit
GAGTACATGGGAAGCAGTGGTATCA154120.43145247080165283No Hit
CATGTACTCTGCGTTGATACCACTG115770.3240932555457263No Hit
GCGTTGATACCACTGCTTCCCATGT100910.2824933092953204No Hit
GTATCAACGCAGAGTACATGGGAAG93400.2614693795281234No Hit
GTATCAACGCAGAGTACTTTTTTTT86770.24290897282286147No Hit
ACGCAGAGTACATGGGAAGCAGTGG82570.23115124911817073No Hit
GCTTCCCATGTACTCTGCGTTGATA72880.20402450085663415No Hit
TATCAACGCAGAGTACATGGGAAGC71520.20021723794273427No Hit
GGTATCAACGCAGAGTACATGGGAA67080.18778764431206119No Hit
ACTCTGCGTTGATACCACTGCTTCC65780.18414834887965692No Hit
GGTATCAACGCAGAGTACTTTTTTT57410.16071688521102315No Hit
GTGGTATCAACGCAGAGTACATGGG51520.1442280774442068No Hit
TATCAACGCAGAGTACTTTTTTTTT49060.1373414107028879No Hit
CAGTGGTATCAACGCAGAGTACATG44310.12404398508448763No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG456.7769893E-414.7744335
CCGGGTT1800.012.143455517
TAGACCG550.003074995712.0881735
CGCAGGA752.0790784E-411.3977392
CGACTCG1604.5474735E-1111.28486214
CGAGAAC600.005886874611.0811353
TCGCCGA1605.4205884E-1010.6916718
CCCGTAC2850.010.67023116
CGGGTTC2151.8189894E-1210.16675718
ACGCAAA1803.947207E-1010.029021
CCGGCGA951.6387146E-410.00390218
CGGCGAT951.6484749E-49.99801619
CCGTACA3300.09.79156117
GACTCGC1651.0180884E-89.7911515
CAATCGG800.00450335449.50370718
ACCGGCG1106.8132744E-59.50357317
CCCGGGT2002.6375346E-109.50317516
GTCTTAG2700.09.5011781
ACGGTAC1502.6588896E-79.4981163
CCATCCG1002.7633607E-49.4978499
Produced by FastQC (version 0.11.5)