##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294605_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3572120 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.19137794922903 31.0 30.0 34.0 26.0 34.0 2 30.256012116054332 31.0 30.0 34.0 26.0 34.0 3 30.31680962565647 31.0 30.0 34.0 26.0 34.0 4 33.839419728340594 35.0 35.0 37.0 28.0 37.0 5 34.22590394499625 37.0 35.0 37.0 30.0 37.0 6 34.27621860407825 37.0 35.0 37.0 30.0 37.0 7 34.24577197854495 37.0 35.0 37.0 30.0 37.0 8 34.15251111384836 37.0 35.0 37.0 29.0 37.0 9 35.27787672306641 39.0 35.0 39.0 28.0 39.0 10 35.40633321388979 39.0 35.0 39.0 28.0 39.0 11 35.621237248468695 39.0 35.0 39.0 29.0 39.0 12 35.42007687311737 39.0 35.0 39.0 28.0 39.0 13 35.26089157139178 39.0 35.0 39.0 27.0 39.0 14 36.20757617325286 39.0 36.0 41.0 27.0 41.0 15 36.17575921301636 39.0 36.0 41.0 27.0 41.0 16 36.279883934470284 39.0 36.0 41.0 27.0 41.0 17 36.274706056907384 39.0 36.0 41.0 27.0 41.0 18 36.149798439022206 39.0 36.0 41.0 27.0 41.0 19 36.40561879220183 39.0 36.0 41.0 27.0 41.0 20 36.54185133758104 39.0 36.0 41.0 27.0 41.0 21 36.57083720591694 39.0 36.0 41.0 28.0 41.0 22 36.586319048632184 39.0 36.0 41.0 28.0 41.0 23 36.56131512939095 39.0 36.0 41.0 28.0 41.0 24 36.61981540373784 39.0 36.0 41.0 28.0 41.0 25 35.408074756727096 39.0 34.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9894194101380762 1101 2 0.9120678803192774 1101 3 1.211420580878663 1101 4 1.0015281840010175 1101 5 0.7115079256700199 1101 6 0.7585018129479124 1101 7 0.7676364684965051 1101 8 0.8635267885225559 1101 9 1.1439175166326123 1101 10 1.1238207345010451 1101 11 0.8443589280817392 1101 12 0.972628865433812 1101 13 1.0470012295567273 1101 14 1.2205772684209961 1101 15 1.2824425958565797 1101 16 1.2645855571650841 1101 17 1.2652154616384124 1101 18 1.2961112070421734 1101 19 1.2354435375867254 1101 20 1.120459542104669 1101 21 0.9798802636072779 1101 22 0.9957300420487272 1101 23 1.0374068675529884 1101 24 1.030638601147217 1101 25 0.9782194174028973 1102 1 0.8562113537569118 1102 2 0.8093703591663299 1102 3 1.0503195999272776 1102 4 0.9629932549730853 1102 5 0.7978578429546346 1102 6 0.6726107314986152 1102 7 0.6505749159029364 1102 8 0.8130901767076892 1102 9 1.1409082138198272 1102 10 0.9824330223158455 1102 11 0.8126211486866666 1102 12 1.0151452947436397 1102 13 1.0174290924217786 1102 14 1.1807590387338465 1102 15 1.2892143856150327 1102 16 1.1066764039300523 1102 17 1.156536154253402 1102 18 1.3216999408703956 1102 19 1.0171604664488427 1102 20 0.29680644102641907 1102 21 0.648519705104242 1102 22 0.7000756075292429 1102 23 0.8351496236062204 1102 24 0.8197335159173562 1102 25 0.8009410705655355 1103 1 0.67449342294357 1103 2 0.6089421135086042 1103 3 0.9442828173526934 1103 4 0.7140768906501336 1103 5 0.559820969229321 1103 6 0.39420954784129236 1103 7 0.31554634177704344 1103 8 0.4554236679271071 1103 9 0.46471792365124287 1103 10 0.62894154246748 1103 11 0.5460581659743795 1103 12 0.6796870100375898 1103 13 0.5815601411271913 1103 14 0.45714432635508473 1103 15 0.785119927416595 1103 16 0.8404121026248035 1103 17 0.7911096258103072 1103 18 0.9130818022008356 1103 19 0.6858169860861238 1103 20 -0.20253434387902303 1103 21 0.24533398712134158 1103 22 -0.2205752412305344 1103 23 0.24425200052425566 1103 24 0.2891540739876959 1103 25 0.3434384703282163 1104 1 0.40036935680255326 1104 2 0.35606361347756277 1104 3 0.580972972771665 1104 4 0.4973875423336622 1104 5 0.30939181834254015 1104 6 0.17470448792945348 1104 7 0.2770720966248348 1104 8 0.3756483114568354 1104 9 0.6493075223764393 1104 10 0.5739927210440783 1104 11 0.4233826418751292 1104 12 0.5042433726956475 1104 13 0.4564715668013335 1104 14 0.779233459444221 1104 15 0.7204792742844148 1104 16 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0.2642688517465217 2207 8 0.36206725824632713 2207 9 -1.206405663379094 2207 10 -0.17555134605340328 2207 11 0.11482386282933987 2207 12 0.49821504875779254 2207 13 0.597646460242828 2207 14 0.8451489001151913 2207 15 0.7866985785605038 2207 16 0.8247923656791514 2207 17 0.7677378128428884 2207 18 0.7994371449272037 2207 19 -0.39196284923436764 2207 20 0.21155704316549873 2207 21 -0.36320534497878043 2207 22 0.23778860916606703 2207 23 -0.5165063336341902 2207 24 -1.594026358824884 2207 25 -0.14470796233788263 2208 1 0.4582485076448961 2208 2 0.1069096549616404 2208 3 0.5959262301482333 2208 4 0.47786217472241077 2208 5 0.3487474518024669 2208 6 0.2325734138229194 2208 7 0.3336643646158777 2208 8 0.4025959207664016 2208 9 -0.17125007816889593 2208 10 -0.013016919622003797 2208 11 0.13226919363284395 2208 12 0.2573515623927918 2208 13 0.4240385158713451 2208 14 0.6793560614502567 2208 15 0.7735213426455019 2208 16 0.5058892802367438 2208 17 0.44655400004432266 2208 18 0.7119733661958634 2208 19 -1.4719424658814972 2208 20 -0.18335321075382893 2208 21 -0.2667755340346645 2208 22 0.13929869994734645 2208 23 0.31660926672575584 2208 24 0.38491914101199143 2208 25 0.4947106566009225 2209 1 0.15602279413677778 2209 2 -1.3522552658277007 2209 3 -0.22284228156552643 2209 4 0.15939948802790838 2209 5 0.061213320592180764 2209 6 0.13541981496248212 2209 7 0.1780269269684709 2209 8 0.3019509422602198 2209 9 -2.10710204989698 2209 10 -0.4341890185476416 2209 11 -0.09650599596206888 2209 12 0.2581794396544055 2209 13 0.41796409091866593 2209 14 0.533766477107342 2209 15 0.672935352245787 2209 16 0.6560537803655109 2209 17 0.35114641913513367 2209 18 0.5944848457670133 2209 19 0.35882641296103657 2209 20 0.2253753789869819 2209 21 0.3273980123121092 2209 22 0.23773724951868047 2209 23 0.24543392870430836 2209 24 0.2600534134862258 2209 25 0.2540696137602012 2210 1 0.11419216964862144 2210 2 0.21074910627933363 2210 3 0.15008703700200599 2210 4 0.21139972395049966 2210 5 0.08266535101142125 2210 6 0.10415853001140363 2210 7 0.08914518632582968 2210 8 0.3017003601832968 2210 9 0.4184415201616005 2210 10 -1.4371911106281203 2210 11 -0.5131101598967405 2210 12 -2.335578332730705 2210 13 -0.7161031840292367 2210 14 -0.4270699735568897 2210 15 -0.6971364510754938 2210 16 -0.1153387553727967 2210 17 -0.00536331941115975 2210 18 0.2980390142175722 2210 19 0.11547399639942313 2210 20 0.2259661564090223 2210 21 0.15772997702028846 2210 22 0.11359852307724339 2210 23 0.11896672323857871 2210 24 0.13013526902879846 2210 25 0.10315167461178021 2211 1 -0.25896801676594805 2211 2 -0.04684318247662844 2211 3 -1.4675946942015052 2211 4 -0.5151563011838505 2211 5 -0.38531594726809004 2211 6 -0.35991375164255857 2211 7 -0.2933247937872707 2211 8 -0.05332226448565791 2211 9 0.21459950599258804 2211 10 0.19386616416035451 2211 11 -0.07133757880530567 2211 12 -0.15147960649017733 2211 13 -0.06294538546816852 2211 14 -0.6438006457133483 2211 15 -0.2341723181255162 2211 16 0.06297720203333768 2211 17 0.0875343067967762 2211 18 0.13567404781923642 2211 19 0.1110413205843912 2211 20 0.10241085637863279 2211 21 -0.026111010080121844 2211 22 -0.00834886379455213 2211 23 -0.4511492577544942 2211 24 -0.1583820715827855 2211 25 -0.030632677917040496 2212 1 -0.36598745709279257 2212 2 -0.26311275390697375 2212 3 -2.4192724327285795 2212 4 -1.2732175719257341 2212 5 -0.6772456218960627 2212 6 -0.39796641868925775 2212 7 -0.24972881569588168 2212 8 -0.254547091850327 2212 9 -0.8286224745532564 2212 10 -0.17400684034019065 2212 11 -0.28491263423347135 2212 12 0.006898225978687833 2212 13 -0.376341495175069 2212 14 -0.05282106049086366 2212 15 0.059122153358060814 2212 16 -1.111865190810775 2212 17 -1.2210050448234355 2212 18 -0.17001318513430164 2212 19 -0.1353277098497614 2212 20 -0.15318828256916106 2212 21 -0.0035069074343923035 2212 22 -0.3946521277910975 2212 23 -0.10743783312881305 2212 24 -0.1329698209197261 2212 25 -0.038442790465737176 2213 1 -3.1956546518540883 2213 2 -1.4297967981638173 2213 3 -2.4235558714313186 2213 4 -1.0699599181591353 2213 5 -0.7606284030330528 2213 6 -0.5577823580319077 2213 7 -0.42824123325185326 2213 8 -0.20215607998571272 2213 9 -0.3384687124349881 2213 10 -0.1874676815799603 2213 11 -0.5320375434311231 2213 12 -0.31382403188966634 2213 13 -0.5756528048030773 2213 14 -0.17596956587195223 2213 15 -0.47110351613015666 2213 16 -1.7018579082115721 2213 17 -1.1181481060122422 2213 18 -0.3467554141174176 2213 19 -0.3094753160374566 2213 20 -0.18099147399589555 2213 21 -0.04500413250294599 2213 22 -0.006612218701732786 2213 23 0.02770370467842298 2213 24 -0.10269553132441445 2213 25 0.018382098998010576 2214 1 -0.9090445418381883 2214 2 -0.6135990024194591 2214 3 -6.19282709924202 2214 4 -2.1188664859317043 2214 5 -1.3681468440033058 2214 6 -0.6662248486444966 2214 7 -0.5809703914478987 2214 8 -0.3645240712958895 2214 9 -0.11379140642003449 2214 10 -0.2439136611685413 2214 11 -0.6786503043846608 2214 12 -0.5857874131536605 2214 13 -4.959160353263481 2214 14 -3.6517775226423765 2214 15 -1.8800852229700595 2214 16 -2.5890296868823484 2214 17 -3.167621503117232 2214 18 -0.957852919203269 2214 19 -0.8185876904373544 2214 20 -0.42481379992221235 2214 21 -0.3510176443300992 2214 22 -0.3398286164513564 2214 23 -0.29261109864059875 2214 24 -0.28849921338332507 2214 25 0.04490877706589913 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1419.0 3 182.0 4 166.0 5 391.0 6 51.0 7 58.0 8 106.0 9 278.0 10 757.0 11 1771.0 12 4098.0 13 7318.0 14 11406.0 15 15842.0 16 20205.0 17 22545.0 18 23457.0 19 24089.0 20 24018.0 21 24393.0 22 24778.0 23 25714.0 24 27425.0 25 29479.0 26 32953.0 27 37417.0 28 43878.0 29 54289.0 30 67049.0 31 84977.0 32 111992.0 33 149951.0 34 202815.0 35 280058.0 36 398275.0 37 606499.0 38 1212021.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83790278398574 21.14744657687032 13.342954704360022 22.67169593478392 2 14.052234765420286 21.69073520979925 41.2779459468241 22.979084077956365 3 18.743856832294917 27.925982549692257 29.0946304827728 24.235530135240026 4 11.96008533935052 18.23149685299241 38.16104175180388 31.647376055853186 5 12.358710301922178 39.40922448494871 34.89033488277254 13.341730330356572 6 30.054285829543954 38.36521717693516 18.161492639000635 13.419004354520252 7 25.334448254224373 33.905108466675635 23.326442113755547 17.434001165344448 8 23.924099707162174 38.40382935739829 21.16368161071449 16.508389324725048 9 24.52005164569529 18.14056447770083 22.046823450776213 35.292560425827666 10 14.80608395908357 31.373653078747182 32.94481492219967 20.875448039969577 11 31.150789426801406 27.601029935584208 22.115351216773877 19.13282942084051 12 20.83633646966746 29.1487750705276 31.258510442510794 18.756378017294143 13 26.34482623389829 24.535944435184888 26.456286406985097 22.662942923931723 14 19.984021478002077 24.622050808562516 28.104662680626767 27.289265032808636 15 24.02734469436676 30.122416895374858 23.993532206044957 21.85670620421342 16 20.46307776172359 31.899876017338524 27.066877244839706 20.570168976098184 17 20.410006992098033 30.91277693862641 28.643174412506887 20.034041656768665 18 20.684370075462038 29.897820524429914 29.72419924086596 19.693610159242088 19 20.6646583909571 29.97448147014074 30.144978530985444 19.215881607916714 20 22.036325457317712 28.821919987853605 28.940722727403877 20.201031827424803 21 21.523910741002293 29.559528734991446 28.940264395367798 19.976296128638463 22 20.75254141566265 29.186472452309236 28.565517650960988 21.495468481067125 23 20.10571190536353 29.742314624582107 29.156831578658387 20.995141891395978 24 20.491775493309312 30.10414580132035 28.556848759565373 20.847229945804962 25 21.27858778125536 28.439918734444742 27.648134265220204 22.6333592190797 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5011.0 1 5011.0 2 5104.5 3 5198.0 4 5198.0 5 5198.0 6 7296.0 7 9394.0 8 9394.0 9 9394.0 10 13438.5 11 17483.0 12 17483.0 13 17483.0 14 27607.0 15 37731.0 16 37731.0 17 37731.0 18 57708.0 19 77685.0 20 77685.0 21 77685.0 22 108920.0 23 140155.0 24 140155.0 25 140155.0 26 180724.0 27 221293.0 28 221293.0 29 221293.0 30 262513.5 31 303734.0 32 303734.0 33 303734.0 34 336809.5 35 369885.0 36 369885.0 37 369885.0 38 387753.5 39 405622.0 40 405622.0 41 405622.0 42 407728.0 43 409834.0 44 409834.0 45 409834.0 46 458467.5 47 507101.0 48 507101.0 49 507101.0 50 456080.0 51 405059.0 52 405059.0 53 405059.0 54 335427.0 55 265795.0 56 265795.0 57 265795.0 58 221258.0 59 176721.0 60 176721.0 61 176721.0 62 143395.0 63 110069.0 64 110069.0 65 110069.0 66 83736.5 67 57404.0 68 57404.0 69 57404.0 70 42220.5 71 27037.0 72 27037.0 73 27037.0 74 20143.5 75 13250.0 76 13250.0 77 13250.0 78 9042.0 79 4834.0 80 4834.0 81 4834.0 82 3112.5 83 1391.0 84 1391.0 85 1391.0 86 850.0 87 309.0 88 309.0 89 309.0 90 191.5 91 74.0 92 74.0 93 74.0 94 48.0 95 22.0 96 22.0 97 22.0 98 25.5 99 29.0 100 29.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10288008241604424 2 0.05512132851080031 3 0.05870463478270607 4 0.0532736862143489 5 0.05027826612767768 6 0.0698744723021623 7 0.06606720938826242 8 0.06231593563486108 9 0.06755092214147342 10 0.0673269654994793 11 0.06125214158538907 12 0.06273585433860004 13 0.06256788685710447 14 0.06517138282028599 15 0.06811081374645868 16 0.06343571884483164 17 0.06651512267225065 18 0.14448002866645018 19 0.07158214169736739 20 0.06542333404252937 21 0.06267986517810152 22 0.06875468909219175 23 0.06769089504271973 24 0.07292588154933205 25 0.07244997368509457 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3572120.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.93962417688529 #Duplication Level Percentage of deduplicated Percentage of total 1 89.19045687344659 66.83899318260784 2 8.215610499285681 12.313495264002837 3 1.458426096608283 3.2788171070875958 4 0.42670886020604376 1.27909606467152 5 0.1961432811071325 0.7349451885494838 6 0.10928496802657672 0.49138646592567514 7 0.07090342154270476 0.37194330342858967 8 0.04261435354347994 0.2554802911271445 9 0.03668952083853194 0.24745490125826264 >10 0.21566924279628605 2.989072446485951 >50 0.01980879212339532 1.023591661034208 >100 0.013950317674663306 2.081994572360133 >500 0.0016971694646670153 0.8441991067931077 >1k 0.0015085950636383766 2.547803000926222 >5k 3.3943388931863467E-4 1.8340959580576328 >10k+ 1.8857438295479702E-4 2.8676314856839022 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 33551 0.9392461619430479 No Hit CCCATGTACTCTGCGTTGATACCAC 30830 0.8630729090848012 No Hit GAGTACATGGGAAGCAGTGGTATCA 15412 0.43145247080165283 No Hit CATGTACTCTGCGTTGATACCACTG 11577 0.3240932555457263 No Hit GCGTTGATACCACTGCTTCCCATGT 10091 0.2824933092953204 No Hit GTATCAACGCAGAGTACATGGGAAG 9340 0.2614693795281234 No Hit GTATCAACGCAGAGTACTTTTTTTT 8677 0.24290897282286147 No Hit ACGCAGAGTACATGGGAAGCAGTGG 8257 0.23115124911817073 No Hit GCTTCCCATGTACTCTGCGTTGATA 7288 0.20402450085663415 No Hit TATCAACGCAGAGTACATGGGAAGC 7152 0.20021723794273427 No Hit GGTATCAACGCAGAGTACATGGGAA 6708 0.18778764431206119 No Hit ACTCTGCGTTGATACCACTGCTTCC 6578 0.18414834887965692 No Hit GGTATCAACGCAGAGTACTTTTTTT 5741 0.16071688521102315 No Hit GTGGTATCAACGCAGAGTACATGGG 5152 0.1442280774442068 No Hit TATCAACGCAGAGTACTTTTTTTTT 4906 0.1373414107028879 No Hit CAGTGGTATCAACGCAGAGTACATG 4431 0.12404398508448763 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.799458024926374E-5 0.0 3 0.0 0.0 0.0 2.799458024926374E-5 0.0 4 0.0 0.0 0.0 2.799458024926374E-5 0.0 5 0.0 0.0 0.0 2.799458024926374E-5 0.0 6 0.0 0.0 0.0 2.799458024926374E-5 0.0 7 0.0 0.0 0.0 5.598916049852748E-5 0.0 8 2.799458024926374E-5 0.0 0.0 5.598916049852748E-5 0.0 9 2.799458024926374E-5 0.0 0.0 1.6796748149558246E-4 0.0 10 2.799458024926374E-5 0.0 0.0 1.959620617448462E-4 0.0 11 2.799458024926374E-5 0.0 0.0 1.959620617448462E-4 0.0 12 2.799458024926374E-5 0.0 0.0 1.959620617448462E-4 0.0 13 2.799458024926374E-5 0.0 0.0 2.519512222433737E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGACCG 45 6.7769893E-4 14.774433 5 CCGGGTT 180 0.0 12.1434555 17 TAGACCG 55 0.0030749957 12.088173 5 CGCAGGA 75 2.0790784E-4 11.397739 2 CGACTCG 160 4.5474735E-11 11.284862 14 CGAGAAC 60 0.0058868746 11.081135 3 TCGCCGA 160 5.4205884E-10 10.69167 18 CCCGTAC 285 0.0 10.670231 16 CGGGTTC 215 1.8189894E-12 10.166757 18 ACGCAAA 180 3.947207E-10 10.02902 1 CCGGCGA 95 1.6387146E-4 10.003902 18 CGGCGAT 95 1.6484749E-4 9.998016 19 CCGTACA 330 0.0 9.791561 17 GACTCGC 165 1.0180884E-8 9.79115 15 CAATCGG 80 0.0045033544 9.503707 18 ACCGGCG 110 6.8132744E-5 9.503573 17 CCCGGGT 200 2.6375346E-10 9.503175 16 GTCTTAG 270 0.0 9.501178 1 ACGGTAC 150 2.6588896E-7 9.498116 3 CCATCCG 100 2.7633607E-4 9.497849 9 >>END_MODULE