FastQCFastQC Report
Wed 25 May 2016
SRR1294605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294605_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3572120
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC340990.9545871919196444No Hit
GTACATGGGAAGCAGTGGTATCAAC328110.9185301725585926No Hit
GTATCAACGCAGAGTACTTTTTTTT219400.6142010906688465No Hit
GAGTACATGGGAAGCAGTGGTATCA164780.4612946933473679No Hit
GGTATCAACGCAGAGTACTTTTTTT157040.4396268882344378No Hit
TATCAACGCAGAGTACTTTTTTTTT135800.38016639978500166No Hit
CATGTACTCTGCGTTGATACCACTG127740.357602768104095No Hit
GCGTTGATACCACTGCTTCCCATGT108560.3039091631860072No Hit
ACGCAGAGTACATGGGAAGCAGTGG98180.27485078888727144No Hit
GTATCAACGCAGAGTACATGGGAAG97150.27196734712159726No Hit
ACGCAGAGTACTTTTTTTTTTTTTT83870.23479054455057502No Hit
TATCAACGCAGAGTACATGGGAAGC77230.2162021432650639No Hit
GCTTCCCATGTACTCTGCGTTGATA77110.2158662083020727No Hit
ACTCTGCGTTGATACCACTGCTTCC73300.2052002732271032No Hit
GGTATCAACGCAGAGTACATGGGAA69860.1955701376213565No Hit
CAGTGGTATCAACGCAGAGTACATG54290.15198257617325286No Hit
GTGGTATCAACGCAGAGTACATGGG54010.15119872792627348No Hit
CATGGGAAGCAGTGGTATCAACGCA43870.12281222355352003No Hit
ACATGGGAAGCAGTGGTATCAACGC41830.11710132918267024No Hit
GCAGTGGTATCAACGCAGAGTACAT38920.10895490633013448No Hit
ATACCACTGCTTCCCATGTACTCTG36690.10271211493454867No Hit
GAGTACTTTTTTTTTTTTTTTTTTT36570.1023761799715575No Hit
GGGAAGCAGTGGTATCAACGCAGAG36360.10178829378632297No Hit
GTACTTTTTTTTTTTTTTTTTTTTT36270.10153634256407959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGG1209.726136E-911.89963610
GTATTAG5050.011.1103261
TTAGACG600.00578759411.1064834
GGACCGC600.00578759411.1064836
ATCGCCG600.00595988711.06279819
TGTACCG951.3303261E-511.0229775
CGTGCAA1303.1726813E-810.9842810
AGGACCG803.701197E-410.7098235
TAGGCGT803.701197E-410.7098236
AAGCGTG951.6108988E-410.0208887
TATCGCG1701.6112608E-89.5198447
TGGACAG5200.09.5198435
TGTATCG2201.6370905E-119.5198435
TCGCGCG1606.434493E-89.5197099
GTCGGCA800.0045864529.48266216
GTCCTGG4300.09.2885311
GTATCGC1951.6225385E-99.2757456
GCGCGCC1856.850314E-99.23180311
TAGGACA3300.09.2313624
GTGTAGA4900.09.1215361
Produced by FastQC (version 0.11.5)