##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294605_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3572120 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.427334188101185 31.0 31.0 34.0 28.0 34.0 2 31.607795090870408 31.0 31.0 34.0 28.0 34.0 3 31.66885882893072 31.0 31.0 34.0 28.0 34.0 4 35.29034802862166 37.0 35.0 37.0 32.0 37.0 5 35.39514098070614 37.0 35.0 37.0 33.0 37.0 6 34.98302576621166 37.0 35.0 37.0 32.0 37.0 7 34.8803794385407 37.0 35.0 37.0 32.0 37.0 8 34.90511936889018 37.0 35.0 37.0 32.0 37.0 9 36.40036448943484 39.0 37.0 39.0 32.0 39.0 10 36.17055893978926 39.0 37.0 39.0 31.0 39.0 11 36.30847032014602 39.0 37.0 39.0 32.0 39.0 12 36.275784688084386 39.0 37.0 39.0 32.0 39.0 13 36.13713677032126 39.0 35.0 39.0 30.0 39.0 14 37.25848487732775 40.0 37.0 41.0 31.0 41.0 15 37.28619167329205 40.0 37.0 41.0 31.0 41.0 16 37.32222489725989 40.0 37.0 41.0 31.0 41.0 17 37.38137184641054 40.0 37.0 41.0 31.0 41.0 18 37.38722271368263 40.0 37.0 41.0 31.0 41.0 19 37.40011561761643 40.0 37.0 41.0 31.0 41.0 20 37.476455718172964 40.0 37.0 41.0 31.0 41.0 21 37.3207319462952 40.0 37.0 41.0 31.0 41.0 22 37.33627453724959 40.0 37.0 41.0 31.0 41.0 23 37.342423826747144 40.0 37.0 41.0 31.0 41.0 24 37.278583306271905 40.0 37.0 41.0 31.0 41.0 25 36.24896839971782 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5256185082429283 1101 2 0.4724953780879595 1101 3 0.37787137705793583 1101 4 0.338316542806389 1101 5 0.38049451219249875 1101 6 0.48462394532286623 1101 7 0.588796562019148 1101 8 0.5252129808316823 1101 9 0.70288949972241 1101 10 0.702587922269025 1101 11 0.7173742946130801 1101 12 0.7266932581697105 1101 13 0.7775450027639224 1101 14 0.9854721920191736 1101 15 0.9546546751702181 1101 16 0.9877000237058056 1101 17 0.9664441159150243 1101 18 0.9152956486158175 1101 19 1.0578227320444924 1101 20 0.9683846541863446 1101 21 1.1797389682301969 1101 22 1.118678662059395 1101 23 1.0353778922904695 1101 24 1.2357657564570275 1101 25 1.137481899252485 1102 1 0.35973812179319253 1102 2 0.3346640883550762 1102 3 0.2972635062465123 1102 4 0.2283200112759971 1102 5 0.2947236936386375 1102 6 0.26593337865006106 1102 7 0.38624240482830885 1102 8 0.35810191649637346 1102 9 0.4788781169862233 1102 10 0.6843172290665791 1102 11 0.5748708306941808 1102 12 0.6234382748509617 1102 13 0.7055345017074117 1102 14 0.8912816055714572 1102 15 0.8089658372713657 1102 16 0.7527126970237958 1102 17 0.8050022866679853 1102 18 0.690375943852743 1102 19 0.7333867017278166 1102 20 0.6922827655748591 1102 21 0.9517998663487006 1102 22 0.9138036006041048 1102 23 0.8914824898164326 1102 24 0.9666092465648575 1102 25 0.9039676379016512 1103 1 0.3549282931402864 1103 2 0.3230326042421581 1103 3 0.2818500807207265 1103 4 0.2240424377735195 1103 5 0.22331198044440725 1103 6 0.2663582061554379 1103 7 0.30216236797736684 1103 8 0.3198991809978864 1103 9 0.1484249839240661 1103 10 0.41160808290475615 1103 11 0.4460838027450862 1103 12 0.5095112572865474 1103 13 0.5973288200871423 1103 14 0.7468003752970418 1103 15 0.6363629793704675 1103 16 0.6242137704863993 1103 17 0.6408447065896254 1103 18 0.599135691690762 1103 19 0.705878713831332 1103 20 0.640881078960831 1103 21 0.8745715972522135 1103 22 0.8194951497921323 1103 23 0.7221594304724306 1103 24 0.8515615633396862 1103 25 0.9932994216725888 1104 1 0.20755032083011216 1104 2 0.18707780198227297 1104 3 0.1027731576438029 1104 4 0.141897407662384 1104 5 0.0990474784680373 1104 6 0.10617847063672059 1104 7 0.206203799239141 1104 8 0.1644146711574379 1104 9 -0.04718106727521132 1104 10 0.40677016564311685 1104 11 0.42100627951502645 1104 12 0.47416507239798733 1104 13 -0.08220637165143785 1104 14 0.2945069831900895 1104 15 0.4797156417739785 1104 16 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0.18959890744081775 2207 3 0.20835379836977452 2207 4 0.19129793014279528 2207 5 0.1497975128417508 2207 6 0.3257694234759896 2207 7 0.26312418972449336 2207 8 0.31399132453294243 2207 9 0.3872256424731759 2207 10 0.5694745517286322 2207 11 0.4838583350629406 2207 12 0.43316672233611087 2207 13 0.5033374204563543 2207 14 0.4657426493526131 2207 15 0.4708098257420801 2207 16 0.6135337095950888 2207 17 0.5346997825624484 2207 18 0.5258059448157226 2207 19 0.6486292707969881 2207 20 0.5644878441851873 2207 21 0.8850384744096829 2207 22 0.7071690128986958 2207 23 0.5943810680975687 2207 24 0.7804435055819283 2207 25 0.7286491349182569 2208 1 0.1215282154644477 2208 2 0.17408802693114822 2208 3 0.16381746575559575 2208 4 0.1852093172933138 2208 5 0.11183986331563744 2208 6 0.25175301538304495 2208 7 0.18123992739182881 2208 8 0.155432589163091 2208 9 0.31619608726759196 2208 10 0.36934656061828264 2208 11 -0.06485222666863422 2208 12 -0.12887251646169062 2208 13 0.015152670124784606 2208 14 -0.39116435773891567 2208 15 0.08716368929564311 2208 16 0.18873262020853332 2208 17 0.20955947361987626 2208 18 0.30513050678405307 2208 19 0.48141544588256835 2208 20 0.36318817581300067 2208 21 0.6552551979628518 2208 22 0.6068922892949189 2208 23 0.5791407909372026 2208 24 0.7176009576103368 2208 25 0.6113232381158653 2209 1 0.0032024614277901264 2209 2 -0.007273456441602377 2209 3 0.04547374903425805 2209 4 -0.548363118498294 2209 5 -0.08042586966642773 2209 6 0.08557931940973162 2209 7 0.06868681395513221 2209 8 0.1491430244984997 2209 9 0.18401448452548408 2209 10 0.2200570194851892 2209 11 0.2790047273606717 2209 12 0.26336072243629616 2209 13 0.343687885377463 2209 14 0.28369559941501876 2209 15 -0.2741423066316244 2209 16 -0.2968264219453687 2209 17 0.08382735593654189 2209 18 -0.247852316070599 2209 19 0.1775644281642954 2209 20 0.23177651223683426 2209 21 0.5524865351225401 2209 22 0.5007037787942963 2209 23 0.45212698507651083 2209 24 0.5614602272884497 2209 25 0.5240874224726397 2210 1 -0.14447847208240105 2210 2 -0.11552906401994179 2210 3 -0.08544057436062502 2210 4 -0.05279805191679543 2210 5 -0.061633584654885 2210 6 -0.43376062127911297 2210 7 -0.16558246875643334 2210 8 -0.24061744401421237 2210 9 0.002060350222023999 2210 10 0.21841357649582704 2210 11 0.13446475127582147 2210 12 0.08541534925291216 2210 13 0.1748645032101379 2210 14 0.09167420356661182 2210 15 0.08121951774297287 2210 16 0.2345748706484443 2210 17 -0.8100107161814449 2210 18 -1.3193761932184387 2210 19 -0.26800355508444085 2210 20 -0.19458335149769113 2210 21 -0.29901969123856276 2210 22 -0.22778580032681361 2210 23 -0.11949922292252779 2210 24 0.24838762192175068 2210 25 0.22020212852208232 2211 1 -0.22232905725230978 2211 2 -0.24622354383399525 2211 3 -0.12645212029651276 2211 4 -0.07742550465057718 2211 5 -0.12338794742847625 2211 6 -0.03581057893278228 2211 7 -0.15148724815699666 2211 8 -0.07471840582993394 2211 9 -0.09402216064643909 2211 10 0.01617574952675227 2211 11 -0.3451633267710221 2211 12 -0.20025697892693017 2211 13 -0.1317374257348618 2211 14 -0.22561280281676233 2211 15 -0.3427521338675845 2211 16 -0.24192720908447995 2211 17 -0.4113375138592943 2211 18 -0.3578721500673012 2211 19 -0.2740703877668693 2211 20 -0.2951500536095608 2211 21 0.010586251373901234 2211 22 -0.07890540230371101 2211 23 -0.7069621334340752 2211 24 -0.0888792707648065 2211 25 -0.8413412315927928 2212 1 -0.22407961907953222 2212 2 -0.3706059560772914 2212 3 -0.18175493569362544 2212 4 -0.09272823381311923 2212 5 -0.11933424880070476 2212 6 -0.1768008267008767 2212 7 -0.21134670736202565 2212 8 -0.3634342257239993 2212 9 -0.18386600959723864 2212 10 -0.5340479842757446 2212 11 -0.2510473631886043 2212 12 -0.3468842280099267 2212 13 -0.35543853095666833 2212 14 -0.2574728843479548 2212 15 -0.2733672127371847 2212 16 -0.3960950876762581 2212 17 -0.614110566202065 2212 18 -0.4115853748396958 2212 19 -0.4945519791109305 2212 20 -0.5487580722031709 2212 21 -0.05422536291394664 2212 22 -0.38328357647706923 2212 23 -0.2923278030554144 2212 24 -0.4813471838683796 2212 25 -0.1850346457686456 2213 1 -0.23861327618515205 2213 2 -0.19525074464862513 2213 3 -0.10469536058248607 2213 4 -0.04135582167101148 2213 5 -0.03823661269722578 2213 6 -0.22963714738893515 2213 7 -0.21895668679525926 2213 8 -0.19940793530348344 2213 9 -0.18254209885842698 2213 10 -0.28373081499216113 2213 11 -0.028622679658987238 2213 12 0.06554623518344016 2213 13 0.10758104465641338 2213 14 -0.540537742795614 2213 15 -0.2487695810669308 2213 16 -0.19362199334583607 2213 17 -0.19882045581286434 2213 18 -0.14375261211633017 2213 19 -0.2638902693857119 2213 20 -0.33079955855109944 2213 21 0.04520093920253032 2213 22 -0.3262347486625501 2213 23 -0.47003061225107245 2213 24 -0.6999779728528068 2213 25 -0.4307832207537601 2214 1 -0.2785979373389402 2214 2 -0.16472812114312063 2214 3 -0.1884858215622458 2214 4 -0.12540207924012492 2214 5 -0.14121132070380327 2214 6 -0.10834983974224599 2214 7 -0.13169286112633216 2214 8 -0.20326531804050774 2214 9 -0.08750692918896874 2214 10 -0.13159359347111632 2214 11 0.05870175165473057 2214 12 -0.14197256375287992 2214 13 -0.22499330116804828 2214 14 -0.10170320674599509 2214 15 -0.06541677338490359 2214 16 -0.1991141872493003 2214 17 -0.3529347438397181 2214 18 -0.22332951411685542 2214 19 -0.4299809586981027 2214 20 -0.32282213547129146 2214 21 -0.43272021061630994 2214 22 -0.7001296154191223 2214 23 -0.9050462966339765 2214 24 -0.4473731482059691 2214 25 -0.7561173547378743 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 10.0 9 20.0 10 69.0 11 210.0 12 528.0 13 1290.0 14 2725.0 15 4701.0 16 7225.0 17 9218.0 18 10903.0 19 12541.0 20 13397.0 21 14594.0 22 15930.0 23 17638.0 24 19942.0 25 22421.0 26 25685.0 27 29297.0 28 35001.0 29 44076.0 30 56275.0 31 74317.0 32 100867.0 33 138331.0 34 191672.0 35 271853.0 36 392489.0 37 631315.0 38 1427578.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.517835751196465 21.481502653526675 12.597743626646176 24.40291796863069 2 13.916637738933742 21.776676035519525 40.08832290068643 24.218363324860306 3 18.540772460004575 27.38243004444684 29.440731197552957 24.636066297995633 4 11.881834845740617 18.129064789976926 38.25668544121687 31.732414923065594 5 12.585266879979123 38.722314952564716 35.03280470037832 13.659613467077836 6 30.417301992520002 38.36447946583911 17.626295686765083 13.5919228548758 7 25.202648990304155 34.04640241165529 23.161836663891787 17.589111934148775 8 24.345224511853143 37.896352006137626 20.923555509510937 16.834867972498298 9 24.634508838051225 17.01696839767857 22.305549855533606 36.0429729087366 10 14.90309246363403 30.821689121320333 33.06356646489424 21.2116519501514 11 31.56532564355831 26.60421058882917 22.086486438031443 19.743977329581075 12 21.122180027519097 28.627978494232327 31.451891272975157 18.79795020527342 13 26.928597243595327 23.546933093176957 26.75127942337835 22.773190239849367 14 20.223057651828164 23.825215609612435 28.67348192832133 27.278244810238068 15 24.28409017422145 29.24102962349768 24.492569140017967 21.982311062262898 16 20.410943871395066 31.26419675945755 27.549733799387365 20.775125569760014 17 20.230932956040938 30.441399068732032 29.175511790060742 20.152156185166284 18 20.408072996713983 29.236030509732913 30.50617758823506 19.84971890531804 19 20.502780440391298 29.291486007094996 31.100423952771706 19.105309599742 20 21.77428317637208 27.976634557753354 30.166346921276006 20.08273534459856 21 21.229971599458583 28.94738831196644 29.79626915706801 20.026370931506964 22 20.383927551584893 28.655875627383836 29.54986259706112 21.41033422397015 23 19.715468605609164 29.095223311429436 30.238466299694945 20.95084178326646 24 20.178745394891546 29.499484899723416 29.716890809939194 20.60487889544584 25 20.975637478149764 27.852456464702 28.69950650103974 22.472399556108492 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2536.0 1 2536.0 2 2891.0 3 3246.0 4 3246.0 5 3246.0 6 5917.5 7 8589.0 8 8589.0 9 8589.0 10 12220.0 11 15851.0 12 15851.0 13 15851.0 14 23594.0 15 31337.0 16 31337.0 17 31337.0 18 50996.5 19 70656.0 20 70656.0 21 70656.0 22 104098.0 23 137540.0 24 137540.0 25 137540.0 26 180169.5 27 222799.0 28 222799.0 29 222799.0 30 265898.5 31 308998.0 32 308998.0 33 308998.0 34 340429.0 35 371860.0 36 371860.0 37 371860.0 38 387330.5 39 402801.0 40 402801.0 41 402801.0 42 404744.5 43 406688.0 44 406688.0 45 406688.0 46 459668.0 47 512648.0 48 512648.0 49 512648.0 50 463018.0 51 413388.0 52 413388.0 53 413388.0 54 341886.5 55 270385.0 56 270385.0 57 270385.0 58 224323.5 59 178262.0 60 178262.0 61 178262.0 62 144835.5 63 111409.0 64 111409.0 65 111409.0 66 84421.5 67 57434.0 68 57434.0 69 57434.0 70 42000.0 71 26566.0 72 26566.0 73 26566.0 74 19726.0 75 12886.0 76 12886.0 77 12886.0 78 8765.0 79 4644.0 80 4644.0 81 4644.0 82 2950.5 83 1257.0 84 1257.0 85 1257.0 86 765.0 87 273.0 88 273.0 89 273.0 90 162.5 91 52.0 92 52.0 93 52.0 94 32.0 95 12.0 96 12.0 97 12.0 98 7.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09722517720569299 2 0.0 3 0.13191046213453075 4 0.012345609889925311 5 0.023319485347636695 6 0.01293349607515985 7 0.0223116804586632 8 0.01973617907573094 9 2.519512222433737E-4 10 0.3915321993662027 11 0.0660112202277639 12 0.0015397019137095057 13 8.398374074779123E-5 14 0.001091788629721286 15 0.0015956910742080334 16 5.598916049852748E-5 17 2.799458024926374E-5 18 3.919241234896924E-4 19 6.718699259823298E-4 20 1.959620617448462E-4 21 1.399729012463187E-4 22 0.004031219555893979 23 2.799458024926374E-5 24 0.0 25 0.0021275880989440445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3572120.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.61370473554682 #Duplication Level Percentage of deduplicated Percentage of total 1 88.4083589196351 64.19658470744632 2 8.85348998847254 12.8576941580413 3 1.5523844515299345 3.3817315859834536 4 0.45600789911717443 1.324496917742861 5 0.19475930163486474 0.7071097211707683 6 0.11768431005467789 0.5127296245390155 7 0.07706035015930245 0.39169462592998056 8 0.05021709793364106 0.29171596176235604 9 0.03769025373140536 0.24631460602740993 >10 0.21215725601364468 2.881673864797501 >50 0.02164742548298608 1.091753667135578 >100 0.014474259772603078 2.107741812505463 >500 0.001743637059490597 0.8573035576959995 >1k 0.0016661420719091705 2.771844995115851 >5k 3.487274103995938E-4 1.927315178909373 >10k+ 3.099799203551945E-4 4.452295015196745 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 34099 0.9545871919196444 No Hit GTACATGGGAAGCAGTGGTATCAAC 32811 0.9185301725585926 No Hit GTATCAACGCAGAGTACTTTTTTTT 21940 0.6142010906688465 No Hit GAGTACATGGGAAGCAGTGGTATCA 16478 0.4612946933473679 No Hit GGTATCAACGCAGAGTACTTTTTTT 15704 0.4396268882344378 No Hit TATCAACGCAGAGTACTTTTTTTTT 13580 0.38016639978500166 No Hit CATGTACTCTGCGTTGATACCACTG 12774 0.357602768104095 No Hit GCGTTGATACCACTGCTTCCCATGT 10856 0.3039091631860072 No Hit ACGCAGAGTACATGGGAAGCAGTGG 9818 0.27485078888727144 No Hit GTATCAACGCAGAGTACATGGGAAG 9715 0.27196734712159726 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 8387 0.23479054455057502 No Hit TATCAACGCAGAGTACATGGGAAGC 7723 0.2162021432650639 No Hit GCTTCCCATGTACTCTGCGTTGATA 7711 0.2158662083020727 No Hit ACTCTGCGTTGATACCACTGCTTCC 7330 0.2052002732271032 No Hit GGTATCAACGCAGAGTACATGGGAA 6986 0.1955701376213565 No Hit CAGTGGTATCAACGCAGAGTACATG 5429 0.15198257617325286 No Hit GTGGTATCAACGCAGAGTACATGGG 5401 0.15119872792627348 No Hit CATGGGAAGCAGTGGTATCAACGCA 4387 0.12281222355352003 No Hit ACATGGGAAGCAGTGGTATCAACGC 4183 0.11710132918267024 No Hit GCAGTGGTATCAACGCAGAGTACAT 3892 0.10895490633013448 No Hit ATACCACTGCTTCCCATGTACTCTG 3669 0.10271211493454867 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 3657 0.1023761799715575 No Hit GGGAAGCAGTGGTATCAACGCAGAG 3636 0.10178829378632297 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3627 0.10153634256407959 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.799458024926374E-5 0.0 5 0.0 0.0 0.0 2.799458024926374E-5 0.0 6 0.0 0.0 0.0 2.799458024926374E-5 0.0 7 0.0 0.0 0.0 8.398374074779123E-5 0.0 8 0.0 0.0 0.0 1.399729012463187E-4 0.0 9 0.0 0.0 0.0 2.2395664199410993E-4 0.0 10 0.0 0.0 0.0 2.799458024926374E-4 0.0 11 0.0 0.0 0.0 2.799458024926374E-4 0.0 12 0.0 0.0 0.0 3.0794038274190114E-4 0.0 13 0.0 0.0 0.0 3.6392954324042864E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGGG 120 9.726136E-9 11.899636 10 GTATTAG 505 0.0 11.110326 1 TTAGACG 60 0.005787594 11.106483 4 GGACCGC 60 0.005787594 11.106483 6 ATCGCCG 60 0.005959887 11.062798 19 TGTACCG 95 1.3303261E-5 11.022977 5 CGTGCAA 130 3.1726813E-8 10.98428 10 AGGACCG 80 3.701197E-4 10.709823 5 TAGGCGT 80 3.701197E-4 10.709823 6 AAGCGTG 95 1.6108988E-4 10.020888 7 TATCGCG 170 1.6112608E-8 9.519844 7 TGGACAG 520 0.0 9.519843 5 TGTATCG 220 1.6370905E-11 9.519843 5 TCGCGCG 160 6.434493E-8 9.519709 9 GTCGGCA 80 0.004586452 9.482662 16 GTCCTGG 430 0.0 9.288531 1 GTATCGC 195 1.6225385E-9 9.275745 6 GCGCGCC 185 6.850314E-9 9.231803 11 TAGGACA 330 0.0 9.231362 4 GTGTAGA 490 0.0 9.121536 1 >>END_MODULE