FastQCFastQC Report
Wed 25 May 2016
SRR1294604_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294604_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5094779
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC222550.4368197325144035No Hit
CCCATGTACTCTGCGTTGATACCAC209510.41122490298401554No Hit
GTATCAACGCAGAGTACTTTTTTTT125740.24680167677538123No Hit
GAGTACATGGGAAGCAGTGGTATCA100630.19751592757997943No Hit
GGTATCAACGCAGAGTACTTTTTTT80020.1570627499249722No Hit
CATGTACTCTGCGTTGATACCACTG77110.15135102032885037No Hit
TATCAACGCAGAGTACTTTTTTTTT71030.13941723478093948No Hit
GCGTTGATACCACTGCTTCCCATGT68570.13458876233885708No Hit
GTATCAACGCAGAGTACATGGGAAG64270.12614874953359115No Hit
ATTCCATTCCATTCCATTCCATTCC59150.11609924591429775No Hit
ACGCAGAGTACATGGGAAGCAGTGG54320.1066189524609409No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAC700.001495847210.8541523
TAACGTG803.7848475E-410.6841355
CCGTACA4800.09.50466417
CCCGTAC4800.09.504477516
CCCCGTA4900.09.50410415
GGGCCGT1106.8672576E-59.4969156
GTCCTAG4400.09.0680881
CTAATAC6500.09.0590423
CGTCGGT1804.2360625E-88.97089519
GTATTAG7000.08.957051
GTACTGG15500.08.9482951
GGGGCGT850.00745944458.9382736
CGCCCTA4800.08.91044716
CAGTGCG1606.780883E-78.9034469
TCGGTCA9650.08.86393115
CTAGGAC4750.08.7975753
CGAACCG1951.6400008E-88.77293312
CGGTCAG9950.08.69253716
TATACGG1107.171797E-48.6336455
AGTACCG1107.171797E-48.6336455
Produced by FastQC (version 0.11.5)