##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294604_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5094779 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.21720942164518 31.0 30.0 33.0 26.0 34.0 2 30.316861241675056 31.0 30.0 34.0 26.0 34.0 3 30.304233804842173 31.0 30.0 34.0 26.0 34.0 4 33.93433709293377 35.0 35.0 37.0 28.0 37.0 5 34.3322652464415 37.0 35.0 37.0 30.0 37.0 6 34.50272838134883 37.0 35.0 37.0 30.0 37.0 7 34.426411037652464 37.0 35.0 37.0 30.0 37.0 8 34.2439411012725 37.0 35.0 37.0 30.0 37.0 9 35.48609939704941 39.0 35.0 39.0 28.0 39.0 10 35.583570160746916 39.0 35.0 39.0 29.0 39.0 11 35.824084420541105 39.0 35.0 39.0 30.0 39.0 12 35.60907607572379 39.0 35.0 39.0 29.0 39.0 13 35.48071035073357 39.0 35.0 39.0 28.0 39.0 14 36.42883606923873 39.0 36.0 41.0 27.0 41.0 15 36.41827957601301 39.0 36.0 41.0 27.0 41.0 16 36.52566244777251 39.0 36.0 41.0 28.0 41.0 17 36.5185679300319 39.0 36.0 41.0 28.0 41.0 18 36.387127488748774 39.0 36.0 41.0 27.0 41.0 19 36.65093814667918 40.0 36.0 41.0 28.0 41.0 20 36.761420858490624 40.0 36.0 41.0 29.0 41.0 21 36.79875496071567 40.0 36.0 41.0 29.0 41.0 22 36.80195823999431 40.0 36.0 41.0 29.0 41.0 23 36.77215007755979 40.0 36.0 41.0 29.0 41.0 24 36.783913885175394 40.0 36.0 41.0 29.0 41.0 25 35.66428612507039 39.0 34.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.875436323023866 1101 2 0.8687056820770955 1101 3 1.1606707108870147 1101 4 0.9903672965589934 1101 5 0.66835245884198 1101 6 0.7171515375541233 1101 7 0.7014340131118999 1101 8 0.8394719049444319 1101 9 1.129929311027304 1101 10 1.0027488132174014 1101 11 0.8193683227196402 1101 12 1.0051803237064405 1101 13 1.0685466937456098 1101 14 1.229525437221362 1101 15 1.3049856668570854 1101 16 1.2531267222506202 1101 17 1.1797662656431598 1101 18 1.1961171671177908 1101 19 1.120098261209165 1101 20 1.0219304062742154 1101 21 0.9004576665623247 1101 22 0.8562944207723078 1101 23 0.8957517372776849 1101 24 0.9350074537867243 1101 25 0.898373637458846 1102 1 0.9442779862959902 1102 2 0.9020416438310512 1102 3 1.18113207441721 1102 4 1.1112451059539339 1102 5 0.8758216508251309 1102 6 0.7789045409710553 1102 7 0.7650590837946964 1102 8 0.845950891391098 1102 9 1.250654709795711 1102 10 1.1212311408313411 1102 11 0.8847013071305696 1102 12 1.1269211855712271 1102 13 1.1711150280893605 1102 14 1.3714174334436464 1102 15 1.462853615657373 1102 16 1.295464645225259 1102 17 1.3585637390013048 1102 18 1.4500311180516832 1102 19 1.1246948589890664 1102 20 0.4471218453417194 1102 21 0.7940254611957016 1102 22 0.809265060558559 1102 23 0.9089812907447268 1102 24 0.9551647068190121 1102 25 1.0527887377301752 1103 1 0.7166839810712098 1103 2 0.7540713562544212 1103 3 0.9425995587018186 1103 4 0.8551840977413647 1103 5 0.6073146587959855 1103 6 0.5131080099908871 1103 7 0.5426444773568377 1103 8 0.6863307893187383 1103 9 0.733314117368252 1103 10 0.7786903412356665 1103 11 0.6683187710737428 1103 12 0.9215773259003299 1103 13 0.8085933603221207 1103 14 0.6825362852735068 1103 15 0.9197511098110525 1103 16 0.9274748616175685 1103 17 0.9462401394748525 1103 18 1.0323168720421734 1103 19 0.8253450929282096 1103 20 -0.05723120006875604 1103 21 0.3741087150674147 1103 22 -0.157764713413016 1103 23 0.3839530218048921 1103 24 0.5132980890111085 1103 25 0.6586465605533292 1104 1 0.2567852705681837 1104 2 0.2610350922445548 1104 3 0.5300845418653779 1104 4 0.3955292997517361 1104 5 0.2484346070212382 1104 6 0.19822746771264121 1104 7 0.20506133206897914 1104 8 0.33392307384927733 1104 9 0.5444744777574257 1104 10 0.5484115233442708 1104 11 0.3000451422164119 1104 12 0.45191006171866377 1104 13 0.31415125881709827 1104 14 0.6015346141258959 1104 15 0.6184454473251151 1104 16 0.48043738818508075 1104 17 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0.26594999354591664 2207 9 -1.2545715282008487 2207 10 -0.24459337562011996 2207 11 0.07796070034887492 2207 12 0.44931448636076254 2207 13 0.5517965743813136 2207 14 0.7489794546314315 2207 15 0.6990112628589458 2207 16 0.6832724919441944 2207 17 0.6273820627555153 2207 18 0.7131223873252921 2207 19 -0.47888395969402353 2207 20 0.1916468935181257 2207 21 -0.31290136568796356 2207 22 0.15082132813628846 2207 23 -0.5314145362812113 2207 24 -1.5989511002128296 2207 25 -0.31052233806655494 2208 1 0.5239189001317364 2208 2 0.21611410599229686 2208 3 0.6735732740530231 2208 4 0.5675531333383788 2208 5 0.442455623870579 2208 6 0.33403492718925065 2208 7 0.41675149377328324 2208 8 0.5684884747610326 2208 9 -0.134644372633133 2208 10 0.08873857499665405 2208 11 0.3277706986468587 2208 12 0.30306350181479047 2208 13 0.5971826313428323 2208 14 0.8488063397361287 2208 15 0.7502164976995829 2208 16 0.5143556520389794 2208 17 0.44420384768275767 2208 18 0.7337084115986556 2208 19 -1.28671672252581 2208 20 -0.08390533393409783 2208 21 -0.1981136762120883 2208 22 0.15345819907624048 2208 23 0.33133691422806066 2208 24 0.5387899461157133 2208 25 0.5394652741331214 2209 1 0.1213528356691107 2209 2 -1.403215724935464 2209 3 -0.17452206540261272 2209 4 0.11270348745814118 2209 5 0.14832718023080105 2209 6 0.15502065419390476 2209 7 0.12647244653599188 2209 8 0.30749582585549007 2209 9 -2.0308432611899647 2209 10 -0.35268900183682206 2209 11 0.00559108402758568 2209 12 0.293029032052857 2209 13 0.42537431734768205 2209 14 0.5447868856090494 2209 15 0.7079357298984021 2209 16 0.6288230990179429 2209 17 0.3989266503486206 2209 18 0.6239247987981855 2209 19 0.4444387293356513 2209 20 0.42922564432898014 2209 21 0.4698118999438776 2209 22 0.36914662222068984 2209 23 0.40069028184161226 2209 24 0.465638797558789 2209 25 0.479128587932685 2210 1 0.1347222899355245 2210 2 0.1796684351417106 2210 3 0.16937186801387938 2210 4 0.3265378298681867 2210 5 0.1235744501698548 2210 6 0.0976683286291049 2210 7 0.10097382378954478 2210 8 0.15369600326083344 2210 9 0.36007042813107404 2210 10 -1.462596362433871 2210 11 -0.45693376394357443 2210 12 -2.3679828125774876 2210 13 -0.7115504638462227 2210 14 -0.52276052220396 2210 15 -0.7317824716863868 2210 16 -0.11739421029806607 2210 17 -0.012139980476440826 2210 18 0.30037579331963826 2210 19 0.19644936308123562 2210 20 0.17686828813432953 2210 21 0.1284628556979115 2210 22 0.10775346859239221 2210 23 0.04510009982672614 2210 24 0.0837994962300499 2210 25 0.0624787155586759 2211 1 -0.2618746475205569 2211 2 0.01100122939448056 2211 3 -1.4655867819518384 2211 4 -0.5650646756889728 2211 5 -0.40478250339719324 2211 6 -0.4078239450401995 2211 7 -0.3710657755898268 2211 8 0.004711299390187662 2211 9 0.13282863475320994 2211 10 0.07562986691290519 2211 11 -0.1622225344443322 2211 12 -0.20290286334056162 2211 13 -0.1971849627697395 2211 14 -0.7391943852295171 2211 15 -0.3312788770948316 2211 16 -0.08494109828679797 2211 17 -0.17889579410930878 2211 18 -0.0020507067092765396 2211 19 -0.06125148699217675 2211 20 0.014985148593240183 2211 21 -0.07545543381093012 2211 22 -0.14749953303229546 2211 23 -0.41535812582042553 2211 24 -0.27260165789801505 2211 25 -0.09758251166324072 2212 1 -0.2900801208323429 2212 2 -0.29527422735610287 2212 3 -2.3103441537023848 2212 4 -1.3472141447804091 2212 5 -0.6375998958568445 2212 6 -0.38368609800700426 2212 7 -0.30281592276797653 2212 8 -0.2785166669515178 2212 9 -0.929004655085059 2212 10 -0.22861442874956595 2212 11 -0.36947265636383264 2212 12 -0.0687309207673934 2212 13 -0.42694077601246505 2212 14 -0.0037749990518989307 2212 15 0.05443808847439868 2212 16 -1.1815056932067236 2212 17 -1.2722124711353686 2212 18 -0.26910785511861945 2212 19 -0.15943673114193757 2212 20 -0.26416923504846324 2212 21 -0.09248411544506041 2212 22 -0.30931724848611 2212 23 -0.10431275183393751 2212 24 -0.17652762828461022 2212 25 0.008819407292556036 2213 1 -3.1738738991715323 2213 2 -1.368693882470268 2213 3 -2.4010181783064155 2213 4 -1.0812773276706622 2213 5 -0.7766167459196609 2213 6 -0.6254923373044932 2213 7 -0.4770304102996761 2213 8 -0.17877765513692623 2213 9 -0.3399748906665465 2213 10 -0.21947906792274097 2213 11 -0.5656864259935475 2213 12 -0.32167229309983014 2213 13 -0.7166331866005606 2213 14 -0.36055709461922447 2213 15 -0.5152663749050319 2213 16 -1.771794724025611 2213 17 -1.173152245872842 2213 18 -0.43463276110436055 2213 19 -0.3065976533515098 2213 20 -0.2966694108116954 2213 21 -0.08658254754594452 2213 22 -0.12940746290723126 2213 23 0.006467174358483874 2213 24 -0.009574525057516325 2213 25 -0.020923432254619456 2214 1 -0.9095838561902951 2214 2 -0.6893325702484816 2214 3 -6.258943964726651 2214 4 -2.038389457042747 2214 5 -1.266644266875275 2214 6 -0.7453289907886074 2214 7 -0.5898549442625836 2214 8 -0.494306967382947 2214 9 -0.17005270528031247 2214 10 -0.16792480917030872 2214 11 -0.787958101534727 2214 12 -0.4321417963611296 2214 13 -5.278206103270101 2214 14 -3.9351985404604477 2214 15 -2.2348089525142214 2214 16 -2.931747046864224 2214 17 -3.3561471376448964 2214 18 -1.08485883101514 2214 19 -0.6931973982935702 2214 20 -0.538907022319215 2214 21 -0.43415003640770067 2214 22 -0.3583788390752787 2214 23 -0.31958701907333875 2214 24 -0.19124147586654772 2214 25 -0.13017791704483272 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1943.0 3 249.0 4 258.0 5 569.0 6 76.0 7 104.0 8 199.0 9 464.0 10 1113.0 11 2489.0 12 5172.0 13 8878.0 14 13851.0 15 19756.0 16 24767.0 17 27996.0 18 29439.0 19 29938.0 20 30426.0 21 31056.0 22 32145.0 23 33369.0 24 36441.0 25 39743.0 26 44615.0 27 50891.0 28 59954.0 29 74365.0 30 93167.0 31 117885.0 32 156653.0 33 211545.0 34 289172.0 35 403156.0 36 578426.0 37 899221.0 38 1745288.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42333984701173 21.318903957364157 13.249179437628419 23.008576757995694 2 14.562417937284543 21.504368888377424 41.3259685216948 22.607244652643228 3 18.307030906783183 27.545837196427563 29.68119137727909 24.465940519510156 4 11.828398312638654 17.8603753850734 39.012641070843294 31.298585231444655 5 12.77961583113788 38.71735687926817 35.24418224691812 13.25884504267583 6 30.097308963176268 39.0811871760288 17.95227907007805 12.869224790716888 7 25.227923588075758 33.622330825694306 23.93824699121616 17.211498595013776 8 23.633824725138755 38.03354898009687 21.55230317813837 16.78032311662601 9 24.366195384994604 17.379540345698217 22.284110471802155 35.97015379750503 10 14.674684052727768 30.578098316093595 33.63330770205257 21.11390992912607 11 31.758744623603118 26.79998821611643 23.022134061315473 18.41913309896498 12 20.492723781810867 28.17947638253645 32.130273529869264 19.197526305783423 13 26.26308254892214 24.396207801420807 27.10122591847649 22.239483731180563 14 19.915871914134907 23.65328088536198 28.71905493092242 27.711792269580688 15 23.074179378982706 30.524446378865644 24.401852697142704 21.999521545008943 16 21.063216517101353 31.6852670768027 26.42769211673086 20.823824289365085 17 19.887993473029205 30.59484170544216 29.02473127212884 20.49243354939979 18 20.340229373550002 29.973765156975645 29.76930360221476 19.916701867259594 19 20.64041373025039 29.28029320951281 30.017127458143793 20.062165602093007 20 22.04687262780654 28.615529712702028 28.950143404465013 20.387454255026423 21 21.95495736131133 29.323633735431947 28.50473915963878 20.216669743617945 22 20.822965296760067 28.9830102669832 29.291178855824434 20.9028455804323 23 20.595049587793003 29.418895567286945 29.18477409045181 20.801280754468245 24 20.76402425945263 28.828194424474425 29.118060940914138 21.28972037515881 25 21.453750437760092 28.39266279715882 28.252265304122222 21.90132146095887 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4570.0 1 4570.0 2 4562.0 3 4554.0 4 4554.0 5 4554.0 6 7516.5 7 10479.0 8 10479.0 9 10479.0 10 16445.5 11 22412.0 12 22412.0 13 22412.0 14 37066.5 15 51721.0 16 51721.0 17 51721.0 18 81806.0 19 111891.0 20 111891.0 21 111891.0 22 158392.0 23 204893.0 24 204893.0 25 204893.0 26 265966.5 27 327040.0 28 327040.0 29 327040.0 30 388644.5 31 450249.0 32 450249.0 33 450249.0 34 498724.5 35 547200.0 36 547200.0 37 547200.0 38 575919.0 39 604638.0 40 604638.0 41 604638.0 42 599457.0 43 594276.0 44 594276.0 45 594276.0 46 613395.0 47 632514.0 48 632514.0 49 632514.0 50 578877.5 51 525241.0 52 525241.0 53 525241.0 54 456328.5 55 387416.0 56 387416.0 57 387416.0 58 329125.5 59 270835.0 60 270835.0 61 270835.0 62 225041.5 63 179248.0 64 179248.0 65 179248.0 66 137072.0 67 94896.0 68 94896.0 69 94896.0 70 69524.5 71 44153.0 72 44153.0 73 44153.0 74 30976.5 75 17800.0 76 17800.0 77 17800.0 78 11886.0 79 5972.0 80 5972.0 81 5972.0 82 3981.0 83 1990.0 84 1990.0 85 1990.0 86 1255.5 87 521.0 88 521.0 89 521.0 90 350.0 91 179.0 92 179.0 93 179.0 94 112.0 95 45.0 96 45.0 97 45.0 98 45.5 99 46.0 100 46.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09768824123676415 2 0.05175886922671229 3 0.054761943550446446 4 0.05040454159051845 5 0.04779402600191294 6 0.06810894054482049 7 0.064183353193534 8 0.061219534743312716 9 0.06563582051351001 10 0.06563582051351001 11 0.060434417273055416 12 0.06129804649033844 13 0.0621813036443779 14 0.06392819001570038 15 0.06655833354106233 16 0.061533581731415626 17 0.06457591192866266 18 0.14269510021926368 19 0.06946326818101434 20 0.0635160033438153 21 0.06200465221357001 22 0.06693126433943454 23 0.06683312465565239 24 0.07488057872578967 25 0.06909033738264211 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5094779.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.2134783871819 #Duplication Level Percentage of deduplicated Percentage of total 1 84.55383201785216 60.21372488956723 2 10.895595106185631 15.518264532196707 3 2.4963240216729417 5.33315750294427 4 0.8303782966697388 2.3653650753230147 5 0.3605279905919068 1.2837226132995438 6 0.2028323750618421 0.8666639374612359 7 0.11962714564483531 0.5963345605629117 8 0.09075610001090278 0.5170446053305076 9 0.06476089431246936 0.4150663692710046 >10 0.3416686807991429 4.432424804211226 >50 0.024291407252018653 1.2060931512749118 >100 0.016180119998095814 2.1797669672768127 >500 0.0013904521868775659 0.6822598662870297 >1k 0.0015294931346355259 2.146546320845476 >5k 1.946627625867022E-4 0.9396276746615673 >10k+ 1.112358643352584E-4 1.3039371294864075 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 22255 0.4368197325144035 No Hit CCCATGTACTCTGCGTTGATACCAC 20951 0.41122490298401554 No Hit GTATCAACGCAGAGTACTTTTTTTT 12574 0.24680167677538123 No Hit GAGTACATGGGAAGCAGTGGTATCA 10063 0.19751592757997943 No Hit GGTATCAACGCAGAGTACTTTTTTT 8002 0.1570627499249722 No Hit CATGTACTCTGCGTTGATACCACTG 7711 0.15135102032885037 No Hit TATCAACGCAGAGTACTTTTTTTTT 7103 0.13941723478093948 No Hit GCGTTGATACCACTGCTTCCCATGT 6857 0.13458876233885708 No Hit GTATCAACGCAGAGTACATGGGAAG 6427 0.12614874953359115 No Hit ATTCCATTCCATTCCATTCCATTCC 5915 0.11609924591429775 No Hit ACGCAGAGTACATGGGAAGCAGTGG 5432 0.1066189524609409 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.9255873512864833E-5 0.0 4 0.0 0.0 0.0 9.81396837821621E-5 0.0 5 0.0 0.0 0.0 1.5702349405145933E-4 0.0 6 0.0 0.0 0.0 1.5702349405145933E-4 0.0 7 0.0 0.0 0.0 2.159073043207566E-4 0.0 8 0.0 0.0 0.0 3.336749248593511E-4 0.0 9 0.0 0.0 0.0 3.7293079837221594E-4 0.0 10 0.0 0.0 0.0 3.925587351286484E-4 0.0 11 0.0 0.0 0.0 3.925587351286484E-4 0.0 12 0.0 0.0 0.0 3.925587351286484E-4 0.0 13 0.0 0.0 0.0 4.318146086415132E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGTAC 70 0.0014958472 10.854152 3 TAACGTG 80 3.7848475E-4 10.684135 5 CCGTACA 480 0.0 9.504664 17 CCCGTAC 480 0.0 9.5044775 16 CCCCGTA 490 0.0 9.504104 15 GGGCCGT 110 6.8672576E-5 9.496915 6 GTCCTAG 440 0.0 9.068088 1 CTAATAC 650 0.0 9.059042 3 CGTCGGT 180 4.2360625E-8 8.970895 19 GTATTAG 700 0.0 8.95705 1 GTACTGG 1550 0.0 8.948295 1 GGGGCGT 85 0.0074594445 8.938273 6 CGCCCTA 480 0.0 8.910447 16 CAGTGCG 160 6.780883E-7 8.903446 9 TCGGTCA 965 0.0 8.863931 15 CTAGGAC 475 0.0 8.797575 3 CGAACCG 195 1.6400008E-8 8.772933 12 CGGTCAG 995 0.0 8.692537 16 TATACGG 110 7.171797E-4 8.633645 5 AGTACCG 110 7.171797E-4 8.633645 5 >>END_MODULE