FastQCFastQC Report
Wed 25 May 2016
SRR1294604_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294604_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5094779
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT289490.5682091411619621No Hit
CCCATGTACTCTGCGTTGATACCAC238020.46718415067660446No Hit
GTACATGGGAAGCAGTGGTATCAAC215450.42288389741733645No Hit
GGTATCAACGCAGAGTACTTTTTTT191840.3765423387353995No Hit
TATCAACGCAGAGTACTTTTTTTTT169410.3325168765907216No Hit
GAGTACATGGGAAGCAGTGGTATCA107310.2106273893332763No Hit
ACGCAGAGTACTTTTTTTTTTTTTT103640.20342393654366558No Hit
CATGTACTCTGCGTTGATACCACTG85480.16777960339398432No Hit
GCGTTGATACCACTGCTTCCCATGT74710.1466403155073066No Hit
ACGCAGAGTACATGGGAAGCAGTGG64530.1266590758892584No Hit
GTATCAACGCAGAGTACATGGGAAG63460.12455888665632013No Hit
ATTCCATTCCATTCCATTCCATTCC54870.10769848898254468No Hit
GTACTTTTTTTTTTTTTTTTTTTTT52800.10363550607396317No Hit
TATCAACGCAGAGTACATGGGAAGC52140.10234006224803863No Hit
ACTCTGCGTTGATACCACTGCTTCC51420.10092685080157549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG1457.2759576E-1212.4759184
AATCGCC1451.2605597E-911.11621318
AAATCGC1701.4370016E-1010.59694417
ATCGCCG1659.640644E-1010.3435319
CCCGTAC4200.010.15882316
GAGTACG750.002626238210.1440371
CCGTACA4300.010.14297417
ACTGTCG856.465236E-410.081168
ACGCCGA900.00109204789.5210956
TACGGCT900.00109204789.5210954
ATACCGT1304.1255953E-69.5210956
CGCAATT1002.8107996E-49.48194112
CCCCGTA4650.09.37943215
GTATTAG7200.09.3779531
AGACCGT1554.131798E-79.2139636
TTAGACA5900.09.1983464
CGCCCTA4150.09.13886116
GTCTTAC3750.09.1296341
TAGGACA6450.09.0044464
GTACCGG850.00730546568.9610316
Produced by FastQC (version 0.11.5)