##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294604_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5094779 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.39612768286907 31.0 31.0 34.0 28.0 34.0 2 31.586256832730133 31.0 31.0 34.0 28.0 34.0 3 31.64496673947977 31.0 31.0 34.0 28.0 34.0 4 35.27070241908432 37.0 35.0 37.0 32.0 37.0 5 35.36749032686207 37.0 35.0 37.0 32.0 37.0 6 34.92538204306801 37.0 35.0 37.0 32.0 37.0 7 34.81597415707335 37.0 35.0 37.0 32.0 37.0 8 34.83296625820276 37.0 35.0 37.0 32.0 37.0 9 36.32322088946351 39.0 37.0 39.0 32.0 39.0 10 36.09790944808401 39.0 35.0 39.0 31.0 39.0 11 36.2283286478177 39.0 37.0 39.0 31.0 39.0 12 36.19446005410637 39.0 35.0 39.0 31.0 39.0 13 36.0608159843636 39.0 35.0 39.0 30.0 39.0 14 37.17447253354856 40.0 37.0 41.0 31.0 41.0 15 37.211961892753344 40.0 37.0 41.0 31.0 41.0 16 37.25545661548813 40.0 37.0 41.0 31.0 41.0 17 37.302398985314184 40.0 37.0 41.0 31.0 41.0 18 37.30835900831027 40.0 37.0 41.0 31.0 41.0 19 37.327794395007125 40.0 37.0 41.0 31.0 41.0 20 37.38491600911443 40.0 37.0 41.0 31.0 41.0 21 37.261555407997086 40.0 37.0 41.0 31.0 41.0 22 37.25387381866809 40.0 37.0 41.0 31.0 41.0 23 37.253814346019716 40.0 37.0 41.0 31.0 41.0 24 37.17438008596644 40.0 37.0 41.0 30.0 41.0 25 36.117856338812736 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4708206956750942 1101 2 0.4273631138011105 1101 3 0.36164327255104567 1101 4 0.29894909548847437 1101 5 0.35328615158561405 1101 6 0.4373929426891081 1101 7 0.4504637846059154 1101 8 0.4369683539570772 1101 9 0.6681435688600246 1101 10 0.8028219839573296 1101 11 0.7356731002073573 1101 12 0.7690549858661058 1101 13 0.7890290563692304 1101 14 0.9218067647757309 1101 15 0.880869428705644 1101 16 0.9295464480235935 1101 17 0.9249280761994498 1101 18 0.8531137658540402 1101 19 0.9976109990934887 1101 20 0.9537147174785048 1101 21 1.173106701976927 1101 22 1.0811012681504977 1101 23 1.0679341126503488 1101 24 1.1861361487942332 1101 25 1.0637270233796414 1102 1 0.4525845520141729 1102 2 0.4574407473201987 1102 3 0.38108958501683077 1102 4 0.32675950247872265 1102 5 0.3937677675173248 1102 6 0.4604497059132342 1102 7 0.5101157751916006 1102 8 0.5520717618786719 1102 9 0.6786335217436559 1102 10 0.8193314973377781 1102 11 0.7113056117755434 1102 12 0.7937220524441386 1102 13 0.8057112729508518 1102 14 1.015379338979983 1102 15 0.9402743340183761 1102 16 0.9723418397962149 1102 17 0.9197747923226629 1102 18 0.8677753982453993 1102 19 1.0160904373332116 1102 20 0.9378915154534084 1102 21 1.1980118303277223 1102 22 1.1869434025296783 1102 23 1.0631044283837525 1102 24 1.1536840677263243 1102 25 1.099984951067924 1103 1 0.30531294342985404 1103 2 0.30247580589222167 1103 3 0.24488075326593162 1103 4 0.21504009272706526 1103 5 0.23796858843817859 1103 6 0.2732848778763852 1103 7 0.33782937723390205 1103 8 0.30949084912788294 1103 9 0.2766551791850418 1103 10 0.5771282159128148 1103 11 0.5398888950988336 1103 12 0.4960902240645524 1103 13 0.6158391884411927 1103 14 0.7696486839127346 1103 15 0.7307186947934312 1103 16 0.7140810782444547 1103 17 0.6402540076959156 1103 18 0.6335113576492191 1103 19 0.7306994388554386 1103 20 0.6369045059913603 1103 21 0.9007444182122057 1103 22 0.8142889666281761 1103 23 0.8286966498295243 1103 24 1.0150475101044307 1103 25 0.8811493679016991 1104 1 0.0869066222582866 1104 2 0.11412745520219403 1104 3 0.049364296839168986 1104 4 0.046406927782612684 1104 5 0.046555084258073975 1104 6 0.037283958816693996 1104 7 0.11389102600838186 1104 8 0.10832972567508392 1104 9 -0.1699025549964972 1104 10 0.2606822754868432 1104 11 0.3020672667500506 1104 12 0.28167662476833755 1104 13 -0.18940599896644983 1104 14 0.15959180939114503 1104 15 0.2882427669939531 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0.19580816484966235 2207 3 0.22863050684285469 2207 4 0.18274094805036611 2207 5 0.16602152739009313 2207 6 0.26223781036048166 2207 7 0.287978817965552 2207 8 0.27736635880802396 2207 9 0.37298911381461153 2207 10 0.5536835213992504 2207 11 0.42968591464929773 2207 12 0.39950345479845595 2207 13 0.45693001014231527 2207 14 0.4139130662615571 2207 15 0.4562190029944446 2207 16 0.5069079541647454 2207 17 0.4896617173889126 2207 18 0.46941929239665825 2207 19 0.5932955991444899 2207 20 0.475361331251996 2207 21 0.7976918488221614 2207 22 0.7103003872344686 2207 23 0.5857284227995834 2207 24 0.7672919534168017 2207 25 0.6737879515652452 2208 1 0.18318480402497173 2208 2 0.1705983790319685 2208 3 0.21205442760303583 2208 4 0.1575077717851059 2208 5 0.13307933472494682 2208 6 0.23243688170854426 2208 7 0.2668153785654326 2208 8 0.17568890555118344 2208 9 0.36096390198212447 2208 10 0.4531315141461647 2208 11 0.076582676559795 2208 12 -0.07430216773154541 2208 13 0.05990115616131675 2208 14 -0.3935848111466953 2208 15 0.24210600732391185 2208 16 0.26187894507583565 2208 17 0.2018882734631049 2208 18 0.3439323188110137 2208 19 0.4321570698400805 2208 20 0.3811286554326756 2208 21 0.6344017089958953 2208 22 0.7202482714899077 2208 23 0.6906431870949561 2208 24 0.7712876997177247 2208 25 0.7279368376847231 2209 1 0.009920225349684841 2209 2 0.002972675393287716 2209 3 0.11542538714243378 2209 4 -0.3972066827680294 2209 5 -0.040411355371190893 2209 6 0.11839897446270697 2209 7 0.10955156488481066 2209 8 0.14618638001022788 2209 9 0.2877415976978668 2209 10 0.28033944557474655 2209 11 0.31603676646729184 2209 12 0.3092036358738781 2209 13 0.36405133422736213 2209 14 0.3492191366324491 2209 15 -0.21625199578785015 2209 16 -0.21667623455552842 2209 17 0.1661878572399047 2209 18 -0.22447710353729633 2209 19 0.20863799124143156 2209 20 0.2127016641513464 2209 21 0.5240265498747974 2209 22 0.5229694957029238 2209 23 0.477528996154156 2209 24 0.5696781172649281 2209 25 0.5593578331372271 2210 1 -0.12537392468810893 2210 2 -0.1854661712250767 2210 3 -0.14591976759822956 2210 4 -0.05331713443510466 2210 5 -0.0654589526726852 2210 6 -0.4920736243170438 2210 7 -0.14859445715791963 2210 8 -0.3454897376814756 2210 9 -0.046808433744374156 2210 10 0.20020346568333736 2210 11 0.03929587507443699 2210 12 0.08536558683748297 2210 13 0.10978125198963085 2210 14 0.04613544376633172 2210 15 -0.012744625446977409 2210 16 0.04605643902664269 2210 17 -0.9851829481427785 2210 18 -1.5486591017536924 2210 19 -0.43153670210037376 2210 20 -0.19268156106429757 2210 21 -0.5130120720996558 2210 22 -0.32022312519150375 2210 23 -0.2630880053417499 2210 24 0.18969876599437185 2210 25 0.11725369938185537 2211 1 -0.22598439195006392 2211 2 -0.18540436104037283 2211 3 -0.16716741702545335 2211 4 -0.08831166464658935 2211 5 -0.15245871741378636 2211 6 -0.09256202654508172 2211 7 -0.15760562938240952 2211 8 -0.11184042290084761 2211 9 -0.06375567653392977 2211 10 0.03979274881499606 2211 11 -0.3339361405044201 2211 12 -0.21939570219078064 2211 13 -0.15937583451444226 2211 14 -0.24965150184585383 2211 15 -0.2876361379158894 2211 16 -0.23426976129488963 2211 17 -0.3191055680128656 2211 18 -0.37661343848605355 2211 19 -0.24444766110180893 2211 20 -0.3221997626755666 2211 21 0.03156463048002678 2211 22 -0.02136414235326356 2211 23 -0.6433761940318234 2211 24 -0.0931033556215013 2211 25 -0.7446304348058845 2212 1 -0.15706983671893582 2212 2 -0.302870733779919 2212 3 -0.1469627864670926 2212 4 -0.059779711001539226 2212 5 -0.08033854878268443 2212 6 -0.2009938875009425 2212 7 -0.16265694208144055 2212 8 -0.4177458329612165 2212 9 -0.08434827651551302 2212 10 -0.417257656540329 2212 11 -0.18322364342755293 2212 12 -0.30382342012939745 2212 13 -0.23326885658708107 2212 14 -0.23008900987419167 2212 15 -0.1050074773955103 2212 16 -0.30880943471873934 2212 17 -0.4833305011351925 2212 18 -0.3291770651506454 2212 19 -0.41175994539005956 2212 20 -0.5779016620275712 2212 21 -0.05704701901410658 2212 22 -0.36142943670891725 2212 23 -0.31374253982738765 2212 24 -0.49506679743812754 2212 25 -0.1820768885022872 2213 1 -0.1914669458345486 2213 2 -0.1876111055817269 2213 3 -0.11443414241249172 2213 4 -1.4275140685526821E-4 2213 5 -0.04005605314476668 2213 6 -0.12523286345687978 2213 7 -0.2047465820807659 2213 8 -0.2286986937751152 2213 9 -0.2717197948463266 2213 10 -0.19675131313316285 2213 11 -0.03521150791875982 2213 12 0.06192386420627116 2213 13 0.14345632471665226 2213 14 -0.5691953141408703 2213 15 -0.2110638877475708 2213 16 -0.31367975584402785 2213 17 -0.24158643658605428 2213 18 -0.23743867945626818 2213 19 -0.27249548951624547 2213 20 -0.3696045602886642 2213 21 -0.06615153160040421 2213 22 -0.27964801811756246 2213 23 -0.4671084108235277 2213 24 -0.5766869830144898 2213 25 -0.3971297435291561 2214 1 -0.20144718256232252 2214 2 -0.08767190011356263 2214 3 -0.0873508762129589 2214 4 0.06181148523847213 2214 5 0.03258514977538596 2214 6 0.005064130959517854 2214 7 -0.08496198311122782 2214 8 0.023352570832848585 2214 9 -0.05258855807053209 2214 10 0.12180445960692765 2214 11 0.16380855436855057 2214 12 -0.04993265776077749 2214 13 -0.14534744693130364 2214 14 -0.02667985821906882 2214 15 0.015522987702681235 2214 16 -0.17866889003790476 2214 17 -0.2269153260980019 2214 18 -0.07072835860674331 2214 19 -0.20643147090955694 2214 20 -0.25053366819087586 2214 21 -0.3005279933384557 2214 22 -0.5264844669640851 2214 23 -0.7388886118751401 2214 24 -0.3271635756049207 2214 25 -0.5045245417683333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 40.0 10 110.0 11 294.0 12 750.0 13 1976.0 14 4038.0 15 7521.0 16 10814.0 17 14251.0 18 17125.0 19 18898.0 20 20871.0 21 22465.0 22 24147.0 23 26683.0 24 29600.0 25 33890.0 26 39155.0 27 43806.0 28 51685.0 29 64691.0 30 82632.0 31 108361.0 32 146407.0 33 197631.0 34 274034.0 35 382959.0 36 554104.0 37 881381.0 38 2034453.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.199305384878194 21.44725367106135 12.402502704806452 24.950938239254008 2 14.251040133438565 21.829111723982532 40.01704490027929 23.902803242299616 3 17.790880022609986 27.24033744384109 29.933233320564774 25.035549212984154 4 11.681012796385202 17.755757411458152 39.07621511872487 31.48701467343178 5 12.883982998439233 38.124154584629885 35.31597184730007 13.675890569630814 6 30.26912211889744 39.315033264820954 17.402850821657765 13.012993794623839 7 25.17073153493172 33.89108088841456 23.661717047316756 17.27647052933696 8 23.83246100023166 37.944094769535226 21.2778534715978 16.945590758635312 9 24.352685112616314 16.709720562843085 22.445830308176394 36.49176401636421 10 14.58214622635933 30.397189245933887 33.61971673361753 21.40094779408925 11 32.02919814118654 26.29447187621528 22.88995997187425 18.786370010723925 12 20.65022815849557 27.904624095923925 32.18396986360739 19.261177881973126 13 26.572340036731724 23.858404849356567 27.20212201549861 22.3671330984131 14 20.025332024008303 23.18192283527099 29.001104473106267 27.79164066761444 15 23.086211742685357 29.991135907191612 24.767327377877297 22.155324972245733 16 20.954868843027942 31.25903744877348 26.735008605879006 21.051085102319572 17 19.718884598655563 30.251163152217092 29.345097802140856 20.68485444698648 18 20.135111316289162 29.504126002353004 30.330543409093497 20.03021927226434 19 20.433233871547085 28.80979140504134 30.807061538201847 19.949913185209727 20 21.746250270866113 28.058883152284853 29.821848610182055 20.373017966666982 21 21.702556718424944 28.942368973063175 29.160592630918003 20.194481677593878 22 20.504376688673606 28.649188700596024 29.971293162080283 20.87514144865009 23 20.269154129641816 28.94374551501381 30.03837656454376 20.748723790800618 24 20.46924900962338 28.40403872277875 29.937569421558813 21.18914284603905 25 21.145768744063677 27.9643346517641 29.106091377831795 21.78380522634043 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2819.0 1 2819.0 2 3227.0 3 3635.0 4 3635.0 5 3635.0 6 7620.5 7 11606.0 8 11606.0 9 11606.0 10 16782.5 11 21959.0 12 21959.0 13 21959.0 14 33562.5 15 45166.0 16 45166.0 17 45166.0 18 74285.5 19 103405.0 20 103405.0 21 103405.0 22 152805.0 23 202205.0 24 202205.0 25 202205.0 26 264487.0 27 326769.0 28 326769.0 29 326769.0 30 390712.0 31 454655.0 32 454655.0 33 454655.0 34 501529.5 35 548404.0 36 548404.0 37 548404.0 38 574085.0 39 599766.0 40 599766.0 41 599766.0 42 595969.5 43 592173.0 44 592173.0 45 592173.0 46 614277.5 47 636382.0 48 636382.0 49 636382.0 50 584854.0 51 533326.0 52 533326.0 53 533326.0 54 462818.0 55 392310.0 56 392310.0 57 392310.0 58 333281.5 59 274253.0 60 274253.0 61 274253.0 62 227655.0 63 181057.0 64 181057.0 65 181057.0 66 138292.5 67 95528.0 68 95528.0 69 95528.0 70 69763.5 71 43999.0 72 43999.0 73 43999.0 74 30723.0 75 17447.0 76 17447.0 77 17447.0 78 11522.5 79 5598.0 80 5598.0 81 5598.0 82 3664.5 83 1731.0 84 1731.0 85 1731.0 86 1088.5 87 446.0 88 446.0 89 446.0 90 279.5 91 113.0 92 113.0 93 113.0 94 67.5 95 22.0 96 22.0 97 22.0 98 13.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0935467465811569 2 0.0 3 0.13272410834699602 4 0.011894529674398045 5 0.022159940598012202 6 0.015879000835953824 7 0.02465268856607912 8 0.02274877870070517 9 3.9255873512864833E-5 10 0.3810763921261354 11 0.0659302395648565 12 0.0014720952567324314 13 0.0 14 8.243733437701616E-4 15 0.0013150717626809719 16 1.177676205385945E-4 17 5.888381026929725E-5 18 3.336749248593511E-4 19 4.906984189108105E-4 20 2.1590730432075663E-4 21 1.177676205385945E-4 22 0.003415260995619241 23 5.888381026929725E-5 24 0.0 25 0.0016880025610531881 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5094779.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.37270141708414 #Duplication Level Percentage of deduplicated Percentage of total 1 83.88834509618536 58.19561116730982 2 11.33843016460426 15.731550606951034 3 2.62439970068351 5.461850905038064 4 0.865943094556129 2.4029124697131277 5 0.40858974064595666 1.4172487039957902 6 0.20593764739182405 0.8571870553829862 7 0.12475137231180942 0.6058037791931058 8 0.08764555850672574 0.4864167328656532 9 0.06604315737021986 0.41234330131971864 >10 0.34242307026315916 4.374813260628382 >50 0.02625480113443595 1.2391321296511066 >100 0.017569489210312626 2.259992697531623 >500 0.0017340607572582049 0.831129858960386 >1k 0.001506639089488361 2.1455512263139163 >5k 2.2741722105283472E-4 0.9848706654858094 >10k+ 1.989900684212304E-4 2.593585439659432 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 28949 0.5682091411619621 No Hit CCCATGTACTCTGCGTTGATACCAC 23802 0.46718415067660446 No Hit GTACATGGGAAGCAGTGGTATCAAC 21545 0.42288389741733645 No Hit GGTATCAACGCAGAGTACTTTTTTT 19184 0.3765423387353995 No Hit TATCAACGCAGAGTACTTTTTTTTT 16941 0.3325168765907216 No Hit GAGTACATGGGAAGCAGTGGTATCA 10731 0.2106273893332763 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 10364 0.20342393654366558 No Hit CATGTACTCTGCGTTGATACCACTG 8548 0.16777960339398432 No Hit GCGTTGATACCACTGCTTCCCATGT 7471 0.1466403155073066 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6453 0.1266590758892584 No Hit GTATCAACGCAGAGTACATGGGAAG 6346 0.12455888665632013 No Hit ATTCCATTCCATTCCATTCCATTCC 5487 0.10769848898254468 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 5280 0.10363550607396317 No Hit TATCAACGCAGAGTACATGGGAAGC 5214 0.10234006224803863 No Hit ACTCTGCGTTGATACCACTGCTTCC 5142 0.10092685080157549 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.9255873512864833E-5 0.0 4 0.0 0.0 0.0 7.851174702572967E-5 0.0 5 0.0 0.0 0.0 9.81396837821621E-5 0.0 6 0.0 0.0 0.0 9.81396837821621E-5 0.0 7 0.0 0.0 0.0 2.3553524107718901E-4 0.0 8 0.0 0.0 0.0 2.5516317783362145E-4 0.0 9 0.0 0.0 0.0 3.925587351286484E-4 0.0 10 0.0 0.0 0.0 4.1218667188508076E-4 0.0 11 0.0 0.0 0.0 4.1218667188508076E-4 0.0 12 0.0 0.0 0.0 4.1218667188508076E-4 0.0 13 0.0 0.0 0.0 4.7107048215437803E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAGACG 145 7.2759576E-12 12.475918 4 AATCGCC 145 1.2605597E-9 11.116213 18 AAATCGC 170 1.4370016E-10 10.596944 17 ATCGCCG 165 9.640644E-10 10.34353 19 CCCGTAC 420 0.0 10.158823 16 GAGTACG 75 0.0026262382 10.144037 1 CCGTACA 430 0.0 10.142974 17 ACTGTCG 85 6.465236E-4 10.08116 8 ACGCCGA 90 0.0010920478 9.521095 6 TACGGCT 90 0.0010920478 9.521095 4 ATACCGT 130 4.1255953E-6 9.521095 6 CGCAATT 100 2.8107996E-4 9.481941 12 CCCCGTA 465 0.0 9.379432 15 GTATTAG 720 0.0 9.377953 1 AGACCGT 155 4.131798E-7 9.213963 6 TTAGACA 590 0.0 9.198346 4 CGCCCTA 415 0.0 9.138861 16 GTCTTAC 375 0.0 9.129634 1 TAGGACA 645 0.0 9.004446 4 GTACCGG 85 0.0073054656 8.961031 6 >>END_MODULE