FastQCFastQC Report
Wed 25 May 2016
SRR1294603_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294603_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5442464
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC299540.5503757121774255No Hit
CCCATGTACTCTGCGTTGATACCAC280000.5144728564120957No Hit
GAGTACATGGGAAGCAGTGGTATCA137550.252734790712442No Hit
GTATCAACGCAGAGTACTTTTTTTT132800.2440071261840225No Hit
CATGTACTCTGCGTTGATACCACTG104830.1926149626345714No Hit
GCGTTGATACCACTGCTTCCCATGT93650.17207279643925985No Hit
GGTATCAACGCAGAGTACTTTTTTT87720.16117699630167512No Hit
GTATCAACGCAGAGTACATGGGAAG83960.15426836080128412No Hit
TATCAACGCAGAGTACTTTTTTTTT76810.14113092893218954No Hit
ACGCAGAGTACATGGGAAGCAGTGG71910.13212765394497786No Hit
GTACTGGTTCACTATCGGTCAGTCA66090.12143396814384072No Hit
TATCAACGCAGAGTACATGGGAAGC65290.11996404569694903No Hit
ACTCTGCGTTGATACCACTGCTTCC60860.11182435014728623No Hit
GGTATCAACGCAGAGTACATGGGAA60200.11061166412860057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA7950.010.042809515
CCCGTAC8200.09.8526316
CCGTACA8200.09.73680617
TACACCG2002.6739144E-109.4969395
CTAGTAC3300.09.2091533
GTACTGG20100.09.1217191
ATTTCGA1054.4733862E-49.05219415
CGTGCGA850.0074594378.93829510
CGACTAA8250.08.87131217
CTAGGAC3750.08.863813
GCGACTA8050.08.85548716
ACTGATC5600.08.8185058
CGCAAGT1304.3481712E-58.77350212
TAGGACC3600.08.7055274
AGGACCG1554.23058E-68.577885
TAAGCGA7900.08.542212513
CTAGACT4350.08.5144974
CGCGCCT5950.08.46630712
TCTAGAC4600.08.4646633
TATCGCG5650.08.4042937
Produced by FastQC (version 0.11.5)