##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294603_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5442464 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.312000226368056 31.0 30.0 34.0 26.0 34.0 2 30.303893420333143 31.0 30.0 34.0 26.0 34.0 3 30.306629497227725 31.0 30.0 34.0 26.0 34.0 4 33.938199131863804 35.0 35.0 37.0 28.0 37.0 5 34.293764552232226 37.0 35.0 37.0 30.0 37.0 6 34.38559924328393 37.0 35.0 37.0 30.0 37.0 7 34.25850423631649 37.0 35.0 37.0 30.0 37.0 8 34.162037452154024 37.0 35.0 37.0 29.0 37.0 9 35.27927699659566 39.0 35.0 39.0 28.0 39.0 10 35.370904979803264 39.0 35.0 39.0 28.0 39.0 11 35.640040797697516 39.0 35.0 39.0 29.0 39.0 12 35.406846421032824 39.0 35.0 39.0 27.0 39.0 13 35.28266222799085 39.0 35.0 39.0 27.0 39.0 14 36.202353015104926 39.0 36.0 41.0 27.0 41.0 15 36.18797441746973 39.0 36.0 41.0 27.0 41.0 16 36.29437089524157 39.0 36.0 41.0 27.0 41.0 17 36.28186295766035 39.0 36.0 41.0 27.0 41.0 18 36.155198086748946 39.0 36.0 41.0 27.0 41.0 19 36.414659426318664 39.0 36.0 41.0 27.0 41.0 20 36.539341739329835 39.0 36.0 41.0 27.0 41.0 21 36.56844252897217 39.0 36.0 41.0 28.0 41.0 22 36.57300921053405 39.0 36.0 41.0 28.0 41.0 23 36.543037859322546 39.0 36.0 41.0 28.0 41.0 24 36.59149017797821 39.0 36.0 41.0 28.0 41.0 25 35.38724794504842 39.0 34.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1182407664588787 1101 2 1.0965509942098421 1101 3 1.3880922743700133 1101 4 1.2628055793505766 1101 5 0.8731762520418656 1101 6 0.8977965673457575 1101 7 0.9027182699007241 1101 8 1.055691262557957 1101 9 1.3389227768520016 1101 10 1.2968408175320079 1101 11 1.0848354333259778 1101 12 1.2493608930885642 1101 13 1.3215078062093113 1101 14 1.4083812152348045 1101 15 1.5737197469944562 1101 16 1.4869233073569816 1101 17 1.4429217542281378 1101 18 1.4830640993474873 1101 19 1.3462698925138383 1101 20 1.2491617510361905 1101 21 1.1955021407521116 1101 22 1.1004051346086001 1101 23 1.1069531447647947 1101 24 1.1284879527977552 1101 25 1.1649027444944338 1102 1 0.8745304963163996 1102 2 0.8572104853106737 1102 3 1.1195279548752097 1102 4 0.9707804658329948 1102 5 0.8155700278262046 1102 6 0.6612579375942786 1102 7 0.6309232348779759 1102 8 0.716657775324947 1102 9 1.086654239806947 1102 10 0.8865063199708558 1102 11 0.7173604100825273 1102 12 0.9104622597138246 1102 13 0.9284411942914943 1102 14 1.2098881677538742 1102 15 1.286979782636017 1102 16 1.1121712121875973 1102 17 1.1667666789940156 1102 18 1.2604480611483169 1102 19 0.972178012801038 1102 20 0.22371149690805225 1102 21 0.5078741888265199 1102 22 0.6731988140195568 1102 23 0.7525841463910368 1102 24 0.8175660975256633 1102 25 0.8521856460769328 1103 1 0.7595334893545136 1103 2 0.7648024335370387 1103 3 0.9757932355420031 1103 4 0.8444428693564845 1103 5 0.6657261578142624 1103 6 0.4712253467348404 1103 7 0.5040721478630275 1103 8 0.6116143284383924 1103 9 0.6744468992556989 1103 10 0.6698307334128302 1103 11 0.6137109900576903 1103 12 0.7638599274219047 1103 13 0.7307944420339609 1103 14 0.6899965822454632 1103 15 0.9103281403828873 1103 16 0.8280447465328251 1103 17 0.9070786109659537 1103 18 0.9910837970211333 1103 19 0.8007360882183079 1103 20 -0.05240595409860305 1103 21 0.3649971184140455 1103 22 -0.14696039556326213 1103 23 0.39343009649613236 1103 24 0.5164961661240923 1103 25 0.5748500513370232 1104 1 0.4206963395116965 1104 2 0.37949196833814725 1104 3 0.6592698846401923 1104 4 0.4508913321108423 1104 5 0.2935005602680292 1104 6 0.20144967630966448 1104 7 0.18148020070331228 1104 8 0.2932715245381843 1104 9 0.5738901412441066 1104 10 0.6298626996871448 1104 11 0.3432599823100304 1104 12 0.47873125909507763 1104 13 0.4028759469829879 1104 14 0.7262730995687505 1104 15 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0.2815832198255137 2207 7 0.3151212568866981 2207 8 0.3475729532620022 2207 9 -1.2961185323347877 2207 10 -0.2201907370527465 2207 11 0.10799412928933805 2207 12 0.39624118657457785 2207 13 0.49291554911217617 2207 14 0.7320068065814311 2207 15 0.74621366109767 2207 16 0.7678433955893738 2207 17 0.7980478536974047 2207 18 0.7984853373910425 2207 19 -0.3029833046357453 2207 20 0.17519791847796995 2207 21 -0.3063914863470032 2207 22 0.2236585362037431 2207 23 -0.487244884598546 2207 24 -1.5324059390054572 2207 25 -0.16045462555539558 2208 1 0.5313835055867067 2208 2 0.22944132073655865 2208 3 0.7173281159162954 2208 4 0.5421154981069165 2208 5 0.463067555564713 2208 6 0.36359865638087996 2208 7 0.4126527002373379 2208 8 0.5652942655311435 2208 9 -0.0979528701529091 2208 10 0.14055492914825862 2208 11 0.3090849772586708 2208 12 0.3257870787016728 2208 13 0.616500859447207 2208 14 0.8317700716020298 2208 15 0.7518567934190798 2208 16 0.5484174360147875 2208 17 0.5443295624124147 2208 18 0.7798043574301659 2208 19 -1.3539723789913722 2208 20 -0.060827576581438336 2208 21 -0.1791731251888038 2208 22 0.19007579409387176 2208 23 0.42085997898189476 2208 24 0.5360440545253979 2208 25 0.6205358851279144 2209 1 0.09511848537779599 2209 2 -1.5151085223455532 2209 3 -0.26946272872872257 2209 4 0.08430556796425748 2209 5 -0.03490769118640458 2209 6 0.06454660859388639 2209 7 0.10401839509783883 2209 8 0.26441641672473537 2209 9 -1.9861137051623743 2209 10 -0.41677673508738167 2209 11 -0.13668272104051482 2209 12 0.2388364481539611 2209 13 0.30637213935909813 2209 14 0.5493171807816424 2209 15 0.7039361122786048 2209 16 0.6014248917125187 2209 17 0.37975629938237887 2209 18 0.6803217163340207 2209 19 0.41527990348396315 2209 20 0.30698443287192845 2209 21 0.37598101512458726 2209 22 0.21945928172694096 2209 23 0.2688116949754331 2209 24 0.34531936911483996 2209 25 0.3572352873586766 2210 1 0.10214629013705334 2210 2 0.1696179580249293 2210 3 0.07518877530289103 2210 4 0.17861187800408374 2210 5 0.10579856789822628 2210 6 0.010602450456282497 2210 7 0.03610542482815049 2210 8 0.07584000725065465 2210 9 0.2747141357583658 2210 10 -1.4989265316411746 2210 11 -0.4901495140199472 2210 12 -2.3604961076160436 2210 13 -0.7977524352757897 2210 14 -0.5299142112508832 2210 15 -0.788714325983932 2210 16 -0.13069346923812475 2210 17 0.0013685211289882204 2210 18 0.3199428648176834 2210 19 0.2288271727536184 2210 20 0.24828024613425725 2210 21 0.24008576439152307 2210 22 0.15905043754646897 2210 23 0.10898606422789925 2210 24 0.13248986068875723 2210 25 0.05419594203463873 2211 1 -0.2846202407622265 2211 2 -0.05348532636660153 2211 3 -1.4258969401849377 2211 4 -0.5773700112820279 2211 5 -0.45025204799227225 2211 6 -0.406525663610644 2211 7 -0.37260941600544584 2211 8 -0.10759202970234583 2211 9 0.16719087376910124 2211 10 0.11102648714744845 2211 11 -0.2384572845311368 2211 12 -0.1871844152062323 2211 13 -0.23306299512198336 2211 14 -0.8134360032258243 2211 15 -0.3399997856789625 2211 16 -0.07896924633002556 2211 17 -0.1482374386113321 2211 18 -0.03496229049336108 2211 19 0.0023866381389439084 2211 20 0.04691410308863908 2211 21 -0.16880954817757754 2211 22 -0.256079959679397 2211 23 -0.45728125427105226 2211 24 -0.30899651098187064 2211 25 -0.18927013193232511 2212 1 -0.3859343668654134 2212 2 -0.3029234701087802 2212 3 -2.4185245230587 2212 4 -1.3509657999335616 2212 5 -0.5460277006261833 2212 6 -0.3631742829159492 2212 7 -0.25703996397650997 2212 8 -0.2728785663769244 2212 9 -0.8298165632086167 2212 10 -0.18124834359218056 2212 11 -0.3021872212861254 2212 12 0.08225710751160875 2212 13 -0.3734986983389845 2212 14 -0.09761360809524433 2212 15 0.027100642370783135 2212 16 -1.0598197234256972 2212 17 -1.2657401078636283 2212 18 -0.24000280819674913 2212 19 -0.14459022649476339 2212 20 -0.19506598588181845 2212 21 -0.0516918236499464 2212 22 -0.3555528117795106 2212 23 -0.05145068405044384 2212 24 -0.17639360570905183 2212 25 0.004789573459767382 2213 1 -3.2156248218635426 2213 2 -1.394374916807653 2213 3 -2.441508152497949 2213 4 -1.0618875483708123 2213 5 -0.8137257153433382 2213 6 -0.5754502047286323 2213 7 -0.37251034534764926 2213 8 -0.20360724343561287 2213 9 -0.2686178430594026 2213 10 -0.10706363357441262 2213 11 -0.46825935548224606 2213 12 -0.21806194503935217 2213 13 -0.5704683048769894 2213 14 -0.23765057784336108 2213 15 -0.3018148010393489 2213 16 -1.5930695249783113 2213 17 -1.0100158090205866 2213 18 -0.2815051992418418 2213 19 -0.19828559120171718 2213 20 -0.2216545246650412 2213 21 -0.005178398232374093 2213 22 -0.03912980761925411 2213 23 0.03838665254772877 2213 24 0.005870629361155011 2213 25 0.02772473121353869 2214 1 -0.9106381307744158 2214 2 -0.6288155725462019 2214 3 -6.617010506371805 2214 4 -2.176625948560254 2214 5 -1.2171241046321128 2214 6 -0.7614710704239798 2214 7 -0.5541491479735896 2214 8 -0.4103396090080551 2214 9 -0.19796329978686344 2214 10 -0.26377533518454044 2214 11 -0.7913443475094439 2214 12 -0.5936681496011573 2214 13 -5.017818435247371 2214 14 -3.792991468068408 2214 15 -2.2282146980171547 2214 16 -2.915337128196775 2214 17 -3.47424768876067 2214 18 -1.1759795157625845 2214 19 -0.7114556698486396 2214 20 -0.43829875510014915 2214 21 -0.5001281548014092 2214 22 -0.4465661957549045 2214 23 -0.36062006159108506 2214 24 -0.3128952004083416 2214 25 -0.1214729777812451 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2040.0 3 238.0 4 257.0 5 606.0 6 82.0 7 101.0 8 221.0 9 506.0 10 1274.0 11 2951.0 12 6085.0 13 10586.0 14 16677.0 15 23654.0 16 29002.0 17 32450.0 18 34242.0 19 35058.0 20 35488.0 21 36604.0 22 37417.0 23 38966.0 24 41828.0 25 45619.0 26 51244.0 27 58597.0 28 68172.0 29 83886.0 30 104008.0 31 130477.0 32 171955.0 33 230372.0 34 311552.0 35 429338.0 36 607426.0 37 925688.0 38 1837797.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.06693698238712 20.754008709423903 13.13529512705213 23.043759181136853 2 14.724807097212194 21.481979470396755 40.79784809961363 22.995365332777414 3 18.576361823353444 27.667280941973782 29.45304250118394 24.30331473348884 4 12.17009827333215 17.784314871741703 38.30865745811462 31.73692939681153 5 12.728970622567736 38.95839805584304 34.93839029833324 13.374241023255983 6 30.24191843214145 38.31809913036891 18.161367698880902 13.278614738608729 7 25.67751472218596 33.48287482970434 23.528617943876434 17.310992504233262 8 24.121950748679275 37.58437062496725 21.462813313244762 16.83086531310871 9 25.05081400776951 17.34745639464066 21.821350543018113 35.78037905457172 10 15.224171697656683 30.449152374095323 33.08782053026353 21.238855397984466 11 32.02611074637825 26.662141568766383 22.83465884261061 18.477088842244754 12 21.007641542457588 28.123832552338424 31.538405534915327 19.330120370288657 13 26.760847183409325 24.16143918223268 26.739943374025593 22.3377702603324 14 20.406896168799197 23.826244080338615 28.18109981140186 27.58575993946033 15 23.662634555640725 30.133053964196204 24.137305910492174 22.067005569670897 16 21.329887246555707 31.074458749822814 26.42676431314604 21.168889690475442 17 20.64210181053876 30.321247631017513 28.36728292692613 20.6693676315176 18 21.038253326790247 29.41171275256254 29.231961842537906 20.318072078109306 19 21.26194132911082 29.182211669200015 29.512322526802432 20.043524474886734 20 22.51953413350704 28.13976068330812 28.560898933254936 20.779806249929905 21 22.309159010670868 28.89777377228751 28.323939490377352 20.469127726664265 22 21.535940670443406 28.562516593404414 28.41485827669819 21.486684459453993 23 21.09594697928692 29.01338516704845 28.725129993375536 21.165537860289092 24 21.254799629543893 28.85600807987054 28.28221297695902 21.606979313626546 25 21.823133464330873 28.037340734014016 27.82777163771501 22.311754163940105 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5572.0 1 5572.0 2 5451.5 3 5331.0 4 5331.0 5 5331.0 6 8259.0 7 11187.0 8 11187.0 9 11187.0 10 16856.5 11 22526.0 12 22526.0 13 22526.0 14 36526.5 15 50527.0 16 50527.0 17 50527.0 18 80165.0 19 109803.0 20 109803.0 21 109803.0 22 156331.0 23 202859.0 24 202859.0 25 202859.0 26 264060.0 27 325261.0 28 325261.0 29 325261.0 30 389385.0 31 453509.0 32 453509.0 33 453509.0 34 506113.5 35 558718.0 36 558718.0 37 558718.0 38 589640.0 39 620562.0 40 620562.0 41 620562.0 42 627033.5 43 633505.0 44 633505.0 45 633505.0 46 675648.0 47 717791.0 48 717791.0 49 717791.0 50 654876.0 51 591961.0 52 591961.0 53 591961.0 54 508841.5 55 425722.0 56 425722.0 57 425722.0 58 360627.0 59 295532.0 60 295532.0 61 295532.0 62 250950.5 63 206369.0 64 206369.0 65 206369.0 66 158794.5 67 111220.0 68 111220.0 69 111220.0 70 83710.5 71 56201.0 72 56201.0 73 56201.0 74 40445.0 75 24689.0 76 24689.0 77 24689.0 78 16811.0 79 8933.0 80 8933.0 81 8933.0 82 6119.0 83 3305.0 84 3305.0 85 3305.0 86 2155.5 87 1006.0 88 1006.0 89 1006.0 90 634.5 91 263.0 92 263.0 93 263.0 94 163.0 95 63.0 96 63.0 97 63.0 98 56.0 99 49.0 100 49.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09857667409467477 2 0.05157590385531259 3 0.054975099513749646 4 0.050308095744868504 5 0.04729475472874051 6 0.06625675429364347 7 0.06236145980938046 8 0.05914600445680486 9 0.06451122138795956 10 0.06445609929620114 11 0.05826405098866983 12 0.0596788513438031 13 0.05997283583318144 14 0.06221446756469129 15 0.06509919036671626 16 0.05973397343556154 17 0.06283918460462026 18 0.1418658901556354 19 0.06770830270994903 20 0.0623798338399666 21 0.059954461802595296 22 0.06533805276433616 23 0.06491545006085479 24 0.06914147709566844 25 0.06770830270994903 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5442464.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.012131903763056 #Duplication Level Percentage of deduplicated Percentage of total 1 78.10257345767738 47.65204513823156 2 13.748511742372344 16.776520238121133 3 4.081895078187568 7.471353627831031 4 1.6423461409510045 4.008121575333556 5 0.7920867076266029 2.416344934246585 6 0.41744114341629845 1.5281384462503722 7 0.26306055521766136 1.1234919705531972 8 0.17530614689063131 0.8556641406105326 9 0.1327769601457115 0.7290904865571756 >10 0.5812073126150296 6.247525531643301 >50 0.03519719504265313 1.4757124354183424 >100 0.022691381249209944 2.692525090333252 >500 0.0021331247909134226 0.9425015869097149 >1k 0.0022854839150317887 2.873551438139251 >5k 3.352043075283066E-4 1.4323693440724534 >10k+ 1.5236559433104847E-4 1.7750440157485659 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 29954 0.5503757121774255 No Hit CCCATGTACTCTGCGTTGATACCAC 28000 0.5144728564120957 No Hit GAGTACATGGGAAGCAGTGGTATCA 13755 0.252734790712442 No Hit GTATCAACGCAGAGTACTTTTTTTT 13280 0.2440071261840225 No Hit CATGTACTCTGCGTTGATACCACTG 10483 0.1926149626345714 No Hit GCGTTGATACCACTGCTTCCCATGT 9365 0.17207279643925985 No Hit GGTATCAACGCAGAGTACTTTTTTT 8772 0.16117699630167512 No Hit GTATCAACGCAGAGTACATGGGAAG 8396 0.15426836080128412 No Hit TATCAACGCAGAGTACTTTTTTTTT 7681 0.14113092893218954 No Hit ACGCAGAGTACATGGGAAGCAGTGG 7191 0.13212765394497786 No Hit GTACTGGTTCACTATCGGTCAGTCA 6609 0.12143396814384072 No Hit TATCAACGCAGAGTACATGGGAAGC 6529 0.11996404569694903 No Hit ACTCTGCGTTGATACCACTGCTTCC 6086 0.11182435014728623 No Hit GGTATCAACGCAGAGTACATGGGAA 6020 0.11061166412860057 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.674806117229255E-5 0.0 5 0.0 0.0 0.0 3.674806117229255E-5 0.0 6 1.8374030586146274E-5 0.0 1.8374030586146274E-5 3.674806117229255E-5 0.0 7 1.8374030586146274E-5 0.0 1.8374030586146274E-5 1.1024418351687765E-4 0.0 8 1.8374030586146274E-5 0.0 1.8374030586146274E-5 1.2861821410302393E-4 0.0 9 1.8374030586146274E-5 0.0 1.8374030586146274E-5 1.469922446891702E-4 0.0 10 1.8374030586146274E-5 0.0 1.8374030586146274E-5 1.469922446891702E-4 1.8374030586146274E-5 11 3.674806117229255E-5 0.0 1.8374030586146274E-5 1.469922446891702E-4 1.8374030586146274E-5 12 3.674806117229255E-5 0.0 1.8374030586146274E-5 1.6536627527531647E-4 1.8374030586146274E-5 13 3.674806117229255E-5 0.0 1.8374030586146274E-5 2.0211433644760901E-4 1.8374030586146274E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCGTA 795 0.0 10.0428095 15 CCCGTAC 820 0.0 9.85263 16 CCGTACA 820 0.0 9.736806 17 TACACCG 200 2.6739144E-10 9.496939 5 CTAGTAC 330 0.0 9.209153 3 GTACTGG 2010 0.0 9.121719 1 ATTTCGA 105 4.4733862E-4 9.052194 15 CGTGCGA 85 0.007459437 8.938295 10 CGACTAA 825 0.0 8.871312 17 CTAGGAC 375 0.0 8.86381 3 GCGACTA 805 0.0 8.855487 16 ACTGATC 560 0.0 8.818505 8 CGCAAGT 130 4.3481712E-5 8.773502 12 TAGGACC 360 0.0 8.705527 4 AGGACCG 155 4.23058E-6 8.57788 5 TAAGCGA 790 0.0 8.5422125 13 CTAGACT 435 0.0 8.514497 4 CGCGCCT 595 0.0 8.466307 12 TCTAGAC 460 0.0 8.464663 3 TATCGCG 565 0.0 8.404293 7 >>END_MODULE