FastQCFastQC Report
Wed 25 May 2016
SRR1294603_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294603_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5442464
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC323490.5943815154312458No Hit
GTATCAACGCAGAGTACTTTTTTTT309540.5687497427635718No Hit
GTACATGGGAAGCAGTGGTATCAAC285060.5237701158886857No Hit
GGTATCAACGCAGAGTACTTTTTTT216210.3972649153030686No Hit
TATCAACGCAGAGTACTTTTTTTTT187910.34526640874427467No Hit
GAGTACATGGGAAGCAGTGGTATCA137450.25255105040658055No Hit
CATGTACTCTGCGTTGATACCACTG119010.21866933800572683No Hit
ACGCAGAGTACTTTTTTTTTTTTTT115870.2128998924016769No Hit
GCGTTGATACCACTGCTTCCCATGT101790.18702925733638293No Hit
ACGCAGAGTACATGGGAAGCAGTGG85040.15625275610458791No Hit
GTATCAACGCAGAGTACATGGGAAG83470.15336803330256296No Hit
ACTCTGCGTTGATACCACTGCTTCC69070.1269094292585123No Hit
TATCAACGCAGAGTACATGGGAAGC68860.12652357461620325No Hit
GGTATCAACGCAGAGTACATGGGAA62830.11544403417275703No Hit
GTACTGGTTCACTATCGGTCAGTCA61410.11283492182952429No Hit
GCTTCCCATGTACTCTGCGTTGATA58830.10809442193829855No Hit
GTACTTTTTTTTTTTTTTTTTTTTT57770.10614677469616703No Hit
GAGTACTTTTTTTTTTTTTTTTTTT55390.10177375541666421No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG803.7005224E-410.7102454
CCCGTAC8400.010.046864516
CCCCGTA8300.09.82526415
CCGTACA8700.09.70042117
CGCCCTA9200.09.68857516
TGTCCCG9450.09.63826211
ACACCGT2600.09.5202196
GTACTGG19550.09.4860711
CTAGCAC4850.09.40290453
GACCGTC1252.6887354E-59.139417
GTCTAGA5550.09.0819981
TACTGGT17350.09.0353922
AATCGCC1804.3550244E-88.95563318
ATCGCCG1606.93135E-78.88994519
TATCGCG6300.08.7646467
CCTATAC5100.08.7556873
TCCGAAA1201.8009254E-48.69231112
GTCCTAG5750.08.6007031
GGACCGT1554.09321E-68.5989076
GTCCCGC10200.08.55286212
Produced by FastQC (version 0.11.5)