##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294603_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5442464 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33925607959924 31.0 31.0 34.0 28.0 34.0 2 31.52455009348707 31.0 31.0 34.0 28.0 34.0 3 31.582420940221194 31.0 31.0 34.0 28.0 34.0 4 35.21909047078676 37.0 35.0 37.0 32.0 37.0 5 35.31490846792923 37.0 35.0 37.0 32.0 37.0 6 34.86750137437749 37.0 35.0 37.0 32.0 37.0 7 34.74937454799885 37.0 35.0 37.0 32.0 37.0 8 34.76428654374195 37.0 35.0 37.0 32.0 37.0 9 36.24506253050089 39.0 37.0 39.0 31.0 39.0 10 36.008323987076444 39.0 35.0 39.0 30.0 39.0 11 36.149326481534835 39.0 35.0 39.0 31.0 39.0 12 36.11075718645084 39.0 35.0 39.0 30.0 39.0 13 35.97991056991833 39.0 35.0 39.0 30.0 39.0 14 37.06914919418851 40.0 36.0 41.0 30.0 41.0 15 37.10375429217355 40.0 36.0 41.0 31.0 41.0 16 37.136671367968624 40.0 37.0 41.0 31.0 41.0 17 37.18934199656626 40.0 37.0 41.0 31.0 41.0 18 37.19137802289551 40.0 37.0 41.0 31.0 41.0 19 37.205104158704586 40.0 37.0 41.0 30.0 41.0 20 37.267120370479255 40.0 37.0 41.0 31.0 41.0 21 37.10625646765877 40.0 37.0 41.0 30.0 41.0 22 37.11688823297683 40.0 37.0 41.0 30.0 41.0 23 37.11857129417852 40.0 36.0 41.0 30.0 41.0 24 37.03878151513726 40.0 36.0 41.0 30.0 41.0 25 35.94970807340205 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5215157409793925 1101 2 0.5171519715397679 1101 3 0.46032592418860574 1101 4 0.39149529445088405 1101 5 0.42735901106741636 1101 6 0.4650635644751375 1101 7 0.5896982605385332 1101 8 0.5285823561092045 1101 9 0.7132153489317616 1101 10 0.7415170150555142 1101 11 0.7676798281121222 1101 12 0.8343020159874257 1101 13 0.8310900693716263 1101 14 1.022575069476801 1101 15 0.9810634198274926 1101 16 1.0376789238742319 1101 17 1.0438794280817874 1101 18 1.0197054931842118 1101 19 1.1063995705467278 1101 20 0.9900959104527445 1101 21 1.2595580498697245 1101 22 1.1498281533627193 1101 23 1.0985293844364534 1101 24 1.2927777187678942 1101 25 1.2280431740377367 1102 1 0.44084437799887155 1102 2 0.4063730172887574 1102 3 0.3634207341352109 1102 4 0.29772410954485906 1102 5 0.37361309195662784 1102 6 0.3418942384674253 1102 7 0.388082391726023 1102 8 0.44745038286118444 1102 9 0.5715801358211934 1102 10 0.668011422814331 1102 11 0.6519986963277375 1102 12 0.6809980094335586 1102 13 0.7229984527377198 1102 14 0.8979670122263244 1102 15 0.8619745201201781 1102 16 0.8296233806011273 1102 17 0.7472369540084287 1102 18 0.7098462855745922 1102 19 0.896331403337868 1102 20 0.8230914518134966 1102 21 1.0817238246826335 1102 22 1.039281004061813 1102 23 0.9701041834629152 1102 24 1.1026811617018808 1102 25 0.9673260973953433 1103 1 0.31211466528497667 1103 2 0.31165364763989345 1103 3 0.20154742028168116 1103 4 0.19094114717513833 1103 5 0.2454693222592894 1103 6 0.24896254624832181 1103 7 0.3249419704046588 1103 8 0.3537192624626968 1103 9 0.2422410369033301 1103 10 0.4432622138966238 1103 11 0.43991883095523576 1103 12 0.48429149937066285 1103 13 0.602562134293045 1103 14 0.8009938803240004 1103 15 0.7124085698692966 1103 16 0.7094213938069416 1103 17 0.5952391170137474 1103 18 0.7158447895111024 1103 19 0.8034831517367991 1103 20 0.6391705536651742 1103 21 0.895653014047646 1103 22 0.8125981467445413 1103 23 0.8485539446120924 1103 24 0.8862414557029581 1103 25 0.8395063138234349 1104 1 0.21026769884549168 1104 2 0.20276772026723222 1104 3 0.14826127913682186 1104 4 0.1534793073676184 1104 5 0.16293484560974036 1104 6 0.15721259636532636 1104 7 0.23430670405599585 1104 8 0.23547048393729852 1104 9 -0.07921597176113693 1104 10 0.43469355994195524 1104 11 0.4470810774457945 1104 12 0.448865898566261 1104 13 -0.02577397678161475 1104 14 0.34674866044500163 1104 15 0.5358626108833349 1104 16 0.5019381722545049 1104 17 0.54942568077049 1104 18 0.5348108814575809 1104 19 0.6740141928264052 1104 20 0.6015414248954798 1104 21 0.7515650416298669 1104 22 0.7078800350716463 1104 23 0.6303034374461518 1104 24 0.745277833630837 1104 25 0.6657917594393581 1105 1 0.039348523235119615 1105 2 0.08524890396529372 1105 3 -0.0168398359636015 1105 4 -0.021654217490748806 1105 5 0.07835272695471218 1105 6 0.02054738549926327 1105 7 0.025112593016501705 1105 8 -0.054516599331257964 1105 9 0.2094402076889068 1105 10 0.2413085708794398 1105 11 0.25479697521924294 1105 12 -0.1806394312923345 1105 13 -0.4022724552740371 1105 14 -0.7567276190719312 1105 15 -0.11182795289267489 1105 16 0.11014915054885677 1105 17 0.22460789255683977 1105 18 -0.6490955276260877 1105 19 -0.5762731641039451 1105 20 -0.5139132263071318 1105 21 0.12933640854631534 1105 22 0.2911750261561181 1105 23 0.28928527932138337 1105 24 0.43844176907099097 1105 25 0.1734914866538233 1106 1 -0.4487273393855489 1106 2 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2207 2 0.20082035252605124 2207 3 0.21686357368724885 2207 4 0.18884691817603283 2207 5 0.16313511041096262 2207 6 0.2915792339090473 2207 7 0.32501949019791 2207 8 0.2754713719119408 2207 9 0.3854031149201589 2207 10 0.5497225735295928 2207 11 0.446370124569448 2207 12 0.35019232565827707 2207 13 0.41345279064080387 2207 14 0.4082165321451541 2207 15 0.3982223410451198 2207 16 0.5682944983424036 2207 17 0.4943865059470198 2207 18 0.5111692409902986 2207 19 0.6710104004560904 2207 20 0.5541821528362192 2207 21 0.8644656535710098 2207 22 0.7292964093753724 2207 23 0.6444369055098207 2207 24 0.8159094154327633 2207 25 0.7594477366764139 2208 1 0.15704601466434198 2208 2 0.1822607317295173 2208 3 0.16623873875563078 2208 4 0.1678400184594011 2208 5 0.13885064692631488 2208 6 0.275829698367545 2208 7 0.19892948831789425 2208 8 0.2235253584013961 2208 9 0.3520291018286841 2208 10 0.41748041742522446 2208 11 0.01583169276400298 2208 12 -0.08577187043299972 2208 13 0.08648950314419324 2208 14 -0.3971676158817772 2208 15 0.19734988127646602 2208 16 0.24334999613358121 2208 17 0.2712162651669985 2208 18 0.36977314342948375 2208 19 0.5307160893015279 2208 20 0.43818267684483203 2208 21 0.7457563518837347 2208 22 0.6312012357440864 2208 23 0.6363352562591018 2208 24 0.689393114085064 2208 25 0.6817136010938825 2209 1 -0.020303854946909894 2209 2 -0.015080260072359408 2209 3 0.07384368731957736 2209 4 -0.5366243398573332 2209 5 -0.07672329253151844 2209 6 0.13597741090577387 2209 7 0.15501702892841251 2209 8 0.1759075624349009 2209 9 0.21134302869658939 2209 10 0.28818535753178764 2209 11 0.2979590231685947 2209 12 0.32007632945590103 2209 13 0.354523262285376 2209 14 0.39189549494630427 2209 15 -0.27271664037077414 2209 16 -0.2247695508700005 2209 17 0.0982656939350477 2209 18 -0.24205133928087008 2209 19 0.2688516662263254 2209 20 0.25702973816445507 2209 21 0.5173168810911264 2209 22 0.5086320127039485 2209 23 0.5135224475095725 2209 24 0.6208751737563958 2209 25 0.5789647718875841 2210 1 -0.1651045568251881 2210 2 -0.21468087911570777 2210 3 -0.12422509129947912 2210 4 -0.056108744388332354 2210 5 -0.08445614389729883 2210 6 -0.49317673579187726 2210 7 -0.2467370281964989 2210 8 -0.388192082307377 2210 9 -0.11947644972742211 2210 10 0.13288190172765724 2210 11 0.035701186710113575 2210 12 -0.02349889353344281 2210 13 0.06488951213437844 2210 14 0.04248612365304183 2210 15 -0.05030460060230979 2210 16 0.06427322877168251 2210 17 -0.9954577751752325 2210 18 -1.5006136963710617 2210 19 -0.3677988781382808 2210 20 -0.18873345929341667 2210 21 -0.34836846970495117 2210 22 -0.33945535219756096 2210 23 -0.17900281323564826 2210 24 0.16000738900915934 2210 25 0.18507864978023036 2211 1 -0.24983465420934436 2211 2 -0.23093328710135452 2211 3 -0.16367305455255377 2211 4 -0.12317554632150518 2211 5 -0.10339087294283189 2211 6 -0.20275318362075012 2211 7 -0.2168968020959312 2211 8 -0.1317243326786368 2211 9 -0.08557222648445162 2211 10 -0.11287314425537431 2211 11 -0.3824569162501348 2211 12 -0.20812398621432493 2211 13 -0.2016688211166624 2211 14 -0.3299709855448967 2211 15 -0.2839913355691195 2211 16 -0.26273247139777567 2211 17 -0.3830135200161209 2211 18 -0.409630551304609 2211 19 -0.258886066216391 2211 20 -0.33204670157230254 2211 21 -0.018442515666080794 2211 22 -0.14276338245134923 2211 23 -0.6833932359892714 2211 24 -0.20699041155059206 2211 25 -0.8071274063868543 2212 1 -0.22634504940639744 2212 2 -0.3426353260731325 2212 3 -0.20540062510136536 2212 4 -0.15029883323392568 2212 5 -0.09016303258373881 2212 6 -0.25265995251220374 2212 7 -0.2227897725802066 2212 8 -0.37365223216981036 2212 9 -0.14979589066966525 2212 10 -0.5697354237622108 2212 11 -0.27605059996601256 2212 12 -0.32750275322979405 2212 13 -0.3259730267873451 2212 14 -0.31087806509899707 2212 15 -0.2377622585603234 2212 16 -0.38196808432411444 2212 17 -0.6224881539258007 2212 18 -0.3796443303242114 2212 19 -0.5180020947936654 2212 20 -0.6282951246015926 2212 21 -0.1471340811981321 2212 22 -0.4749189612001743 2212 23 -0.30480329361189007 2212 24 -0.47197099398075437 2212 25 -0.2708187157260724 2213 1 -0.18051037630206324 2213 2 -0.15852222684848982 2213 3 -0.08928204771370929 2213 4 -1.7565824394694118E-4 2213 5 -0.04231155466628422 2213 6 -0.21490852039151775 2213 7 -0.30858517142455355 2213 8 -0.2596627406012004 2213 9 -0.21923135704005148 2213 10 -0.19183612494295943 2213 11 0.00846100214682366 2213 12 0.04608850433015732 2213 13 0.13517305562971416 2213 14 -0.4877023176535147 2213 15 -0.11359582481684782 2213 16 -0.1959063023871721 2213 17 -0.1290512340195491 2213 18 -0.15424007706220522 2213 19 -0.205597997148665 2213 20 -0.3302521988938807 2213 21 -0.010429254786451736 2213 22 -0.2813963270150026 2213 23 -0.45884845157505083 2213 24 -0.5599640171513443 2213 25 -0.2751842012368755 2214 1 -0.1427778926498391 2214 2 -0.1298234097698412 2214 3 -0.0960642754513259 2214 4 -0.05043626225904774 2214 5 -0.0738570356531909 2214 6 -0.07949268489500128 2214 7 -0.13501054242672694 2214 8 -0.11902115071320907 2214 9 -0.06857128460919881 2214 10 -0.036581960619656684 2214 11 0.00753920764297078 2214 12 -0.17776961267371405 2214 13 -0.20327244331046757 2214 14 -0.12851061077966364 2214 15 -0.1002045476633171 2214 16 -0.413219718631602 2214 17 -0.4286854440818786 2214 18 -0.33076641914995975 2214 19 -0.5226879465963634 2214 20 -0.3734443845217399 2214 21 -0.46841714308232696 2214 22 -0.7157672529406938 2214 23 -0.883413084652581 2214 24 -0.4932209069619944 2214 25 -0.6842539191452346 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 17.0 9 52.0 10 157.0 11 390.0 12 975.0 13 2260.0 14 4721.0 15 8372.0 16 12333.0 17 16247.0 18 19190.0 19 21319.0 20 23186.0 21 25150.0 22 27249.0 23 30075.0 24 33872.0 25 37983.0 26 43564.0 27 49099.0 28 58236.0 29 72500.0 30 92218.0 31 120146.0 32 160338.0 33 217178.0 34 298213.0 35 416032.0 36 593733.0 37 941088.0 38 2116571.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.56919217555707 21.11444608741071 12.212045640131535 25.104316096900682 2 14.419773837732322 21.708255672430724 39.54201626322195 24.329954226615 3 18.135187765077838 27.291435304265015 29.606954376622298 24.96642255403485 4 12.003680361994142 17.83139796422397 38.28554081548648 31.879380858295402 5 12.915007897036029 38.25668289200964 35.086676264894926 13.741632946059404 6 30.466335525626675 38.48781375246013 17.601193724858916 13.444656997054278 7 25.426584530338005 33.737833923871975 23.355723569107724 17.4798579766823 8 24.425226943102686 37.28536059860493 21.152773193625524 17.136639264666858 9 24.92318728045306 16.55222695333616 21.985525657708337 36.53906010850245 10 15.086186225402637 30.129856667397085 33.20329590468791 21.58066120251236 11 32.196321675850555 26.001916142387287 22.745595602763938 19.05616657899822 12 21.046892039258452 27.753810592579185 31.722155411358415 19.477141956803944 13 27.089132255010277 23.457063465566968 26.916985930818456 22.5368183486043 14 20.521967098411515 23.20043025041103 28.55913885240588 27.718463798771577 15 23.8407241209127 29.473122732850136 24.475976250209925 22.210176896027235 16 21.21919634047435 30.55763080404538 26.800887981781745 21.42228487369852 17 20.439852485851528 29.9035307740377 28.77119376693742 20.88542297317335 18 20.809744019038533 28.810132080560987 29.880792351072206 20.49933154932828 19 21.06410090336403 28.667995116156646 30.32864884927427 19.93925513120506 20 22.143885434055036 27.49154563519588 29.62095230920605 20.743616621543033 21 21.968509058597462 28.475408336019008 29.024260012119534 20.531822593263996 22 21.206153934351736 28.12586647537524 29.201843997407718 21.466135592865303 23 20.745821508859514 28.451590320912164 29.63160926456902 21.1709789056593 24 20.97072979103942 28.356853938530023 29.246855559120117 21.425560711310432 25 21.569527432590576 27.516359120288804 28.691489031355232 22.22262441576539 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3047.0 1 3047.0 2 3447.5 3 3848.0 4 3848.0 5 3848.0 6 7896.0 7 11944.0 8 11944.0 9 11944.0 10 16697.0 11 21450.0 12 21450.0 13 21450.0 14 32363.5 15 43277.0 16 43277.0 17 43277.0 18 72088.5 19 100900.0 20 100900.0 21 100900.0 22 149258.0 23 197616.0 24 197616.0 25 197616.0 26 261925.5 27 326235.0 28 326235.0 29 326235.0 30 391662.5 31 457090.0 32 457090.0 33 457090.0 34 508908.0 35 560726.0 36 560726.0 37 560726.0 38 588400.5 39 616075.0 40 616075.0 41 616075.0 42 623632.5 43 631190.0 44 631190.0 45 631190.0 46 676424.0 47 721658.0 48 721658.0 49 721658.0 50 662244.0 51 602830.0 52 602830.0 53 602830.0 54 516819.5 55 430809.0 56 430809.0 57 430809.0 58 364691.0 59 298573.0 60 298573.0 61 298573.0 62 254110.5 63 209648.0 64 209648.0 65 209648.0 66 160557.5 67 111467.0 68 111467.0 69 111467.0 70 84218.0 71 56969.0 72 56969.0 73 56969.0 74 40581.5 75 24194.0 76 24194.0 77 24194.0 78 16547.0 79 8900.0 80 8900.0 81 8900.0 82 5870.0 83 2840.0 84 2840.0 85 2840.0 86 1852.5 87 865.0 88 865.0 89 865.0 90 564.5 91 264.0 92 264.0 93 264.0 94 153.0 95 42.0 96 42.0 97 42.0 98 24.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09451601333513644 2 0.0 3 0.13354245430011114 4 0.011171410596376935 5 0.021718104152824897 6 0.01238409661506259 7 0.02028492976710549 8 0.01859451895318003 9 1.1024418351687765E-4 10 0.3848991927185922 11 0.06267381832934495 12 0.0012678081104440931 13 1.8374030586146277E-5 14 9.554495904796063E-4 15 0.001065693773996484 16 1.8374030586146276E-4 17 5.5122091758438824E-5 18 5.144728564120957E-4 19 5.32846886998242E-4 20 1.6536627527531647E-4 21 1.8374030586146276E-4 22 0.0028847228020249653 23 1.6536627527531647E-4 24 3.6748061172292554E-5 25 0.001598540660994726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5442464.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.66499508361728 #Duplication Level Percentage of deduplicated Percentage of total 1 76.93437858443347 45.1335494141694 2 14.297141763071828 16.77483502480776 3 4.433051913285746 7.801949060949854 4 1.7348883869889486 4.071088747733255 5 0.8686263078992247 2.547897904120432 6 0.4410116205728627 1.5523166731635047 7 0.28494115841771495 1.1701250160386822 8 0.18260986426580347 0.8570245431498704 9 0.13315052948521178 0.7030147641867889 >10 0.6225732017361704 6.449075339548376 >50 0.037850807520518434 1.5394964880782802 >100 0.024130250063700762 2.734006915030862 >500 0.0025862626975199344 1.0716822495350395 >1k 0.0024600955235480637 3.061299376736427 >5k 3.153968619857587E-4 1.2089330295668161 >10k+ 2.8385717578718287E-4 3.3237054531846075 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 32349 0.5943815154312458 No Hit GTATCAACGCAGAGTACTTTTTTTT 30954 0.5687497427635718 No Hit GTACATGGGAAGCAGTGGTATCAAC 28506 0.5237701158886857 No Hit GGTATCAACGCAGAGTACTTTTTTT 21621 0.3972649153030686 No Hit TATCAACGCAGAGTACTTTTTTTTT 18791 0.34526640874427467 No Hit GAGTACATGGGAAGCAGTGGTATCA 13745 0.25255105040658055 No Hit CATGTACTCTGCGTTGATACCACTG 11901 0.21866933800572683 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 11587 0.2128998924016769 No Hit GCGTTGATACCACTGCTTCCCATGT 10179 0.18702925733638293 No Hit ACGCAGAGTACATGGGAAGCAGTGG 8504 0.15625275610458791 No Hit GTATCAACGCAGAGTACATGGGAAG 8347 0.15336803330256296 No Hit ACTCTGCGTTGATACCACTGCTTCC 6907 0.1269094292585123 No Hit TATCAACGCAGAGTACATGGGAAGC 6886 0.12652357461620325 No Hit GGTATCAACGCAGAGTACATGGGAA 6283 0.11544403417275703 No Hit GTACTGGTTCACTATCGGTCAGTCA 6141 0.11283492182952429 No Hit GCTTCCCATGTACTCTGCGTTGATA 5883 0.10809442193829855 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 5777 0.10614677469616703 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 5539 0.10177375541666421 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.8374030586146274E-5 0.0 3 0.0 0.0 0.0 1.8374030586146274E-5 0.0 4 0.0 0.0 0.0 3.674806117229255E-5 0.0 5 0.0 0.0 0.0 3.674806117229255E-5 0.0 6 0.0 0.0 0.0 3.674806117229255E-5 0.0 7 0.0 0.0 0.0 5.5122091758438824E-5 0.0 8 0.0 0.0 0.0 5.5122091758438824E-5 0.0 9 5.5122091758438824E-5 0.0 0.0 7.34961223445851E-5 0.0 10 5.5122091758438824E-5 0.0 0.0 7.34961223445851E-5 0.0 11 5.5122091758438824E-5 1.8374030586146274E-5 0.0 7.34961223445851E-5 0.0 12 5.5122091758438824E-5 1.8374030586146274E-5 0.0 1.1024418351687765E-4 0.0 13 7.34961223445851E-5 1.8374030586146274E-5 0.0 1.2861821410302393E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGACG 80 3.7005224E-4 10.710245 4 CCCGTAC 840 0.0 10.0468645 16 CCCCGTA 830 0.0 9.825264 15 CCGTACA 870 0.0 9.700421 17 CGCCCTA 920 0.0 9.688575 16 TGTCCCG 945 0.0 9.638262 11 ACACCGT 260 0.0 9.520219 6 GTACTGG 1955 0.0 9.486071 1 CTAGCAC 485 0.0 9.4029045 3 GACCGTC 125 2.6887354E-5 9.13941 7 GTCTAGA 555 0.0 9.081998 1 TACTGGT 1735 0.0 9.035392 2 AATCGCC 180 4.3550244E-8 8.955633 18 ATCGCCG 160 6.93135E-7 8.889945 19 TATCGCG 630 0.0 8.764646 7 CCTATAC 510 0.0 8.755687 3 TCCGAAA 120 1.8009254E-4 8.692311 12 GTCCTAG 575 0.0 8.600703 1 GGACCGT 155 4.09321E-6 8.598907 6 GTCCCGC 1020 0.0 8.552862 12 >>END_MODULE