FastQCFastQC Report
Wed 25 May 2016
SRR1294602_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294602_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770980
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC112290.4052356927873893No Hit
CCCATGTACTCTGCGTTGATACCAC99910.36055835841471245No Hit
GTATCAACGCAGAGTACTTTTTTTT80060.28892305249406347No Hit
GGTATCAACGCAGAGTACTTTTTTT56600.20425986474099417No Hit
GAGTACATGGGAAGCAGTGGTATCA51980.1875870630607222No Hit
TATCAACGCAGAGTACTTTTTTTTT45080.16268612548629005No Hit
CATGTACTCTGCGTTGATACCACTG39250.14164663765166113No Hit
ATTCCATTCCATTCCATTCCATTCC39120.14117748955243273No Hit
GCGTTGATACCACTGCTTCCCATGT33700.12161762264613964No Hit
GTATCAACGCAGAGTACATGGGAAG32890.11869446910479325No Hit
GAATGGAATGGAATGGAATGGAATG30720.11086330467921096No Hit
ACGCAGAGTACATGGGAAGCAGTGG29380.10602747042562559No Hit
GGTATCAACGCAGAGTACATGGGAA29380.10602747042562559No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCACG600.0058895311.080324
GCCTTAC1658.0035534E-1110.94037151
GCGCCTT1106.0171224E-610.36830913
TATTCCG1106.070539E-610.3606325
CGAATAG856.5702235E-410.06317615
TTCCGTA951.6502968E-49.996747
CCCGTAC1106.808919E-59.5039416
TCTAGAC2600.09.4972473
CCTACAC2900.09.4972463
TCGCGCG1106.862957E-59.4970749
CGCGCGC1106.864312E-59.49690310
TATCGCG1106.864312E-59.4969037
TAACACT4800.09.2995554
TGGACCA1752.6844646E-89.2258965
CTAGTGC1252.7648673E-59.1173563
GGAACGA1151.1058977E-49.09088915
AGGCCGA2106.6211214E-109.05137216
CTAGACA3400.08.93874554
CGCGCCT1304.3476124E-58.77318412
GTGCTAG2607.2759576E-128.7700151
Produced by FastQC (version 0.11.5)