FastQCFastQC Report
Wed 25 May 2016
SRR1294602_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294602_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770980
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT174670.6303546037863861No Hit
GGTATCAACGCAGAGTACTTTTTTT136590.49293029902778074No Hit
GTACATGGGAAGCAGTGGTATCAAC114750.41411341835740423No Hit
CCCATGTACTCTGCGTTGATACCAC113980.41133461807735894No Hit
TATCAACGCAGAGTACTTTTTTTTT110410.3984510895062397No Hit
ACGCAGAGTACTTTTTTTTTTTTTT68960.24886502248302045No Hit
GAGTACATGGGAAGCAGTGGTATCA58460.21097229139149326No Hit
CATGTACTCTGCGTTGATACCACTG44000.15878858743116153No Hit
ATTCCATTCCATTCCATTCCATTCC38970.14063616482255376No Hit
ACGCAGAGTACATGGGAAGCAGTGG36560.13193888082916513No Hit
GCGTTGATACCACTGCTTCCCATGT35530.12822178435066292No Hit
GTATCAACGCAGAGTACATGGGAAG33860.12219503569134385No Hit
GAATGGAATGGAATGGAATGGAATG33740.12176197590744069No Hit
GGTATCAACGCAGAGTACATGGGAA31970.11537434409486898No Hit
GAGTACTTTTTTTTTTTTTTTTTTT29240.10552223401107189No Hit
GTGGTATCAACGCAGAGTACATGGG28160.10162469595594338No Hit
TATCAACGCAGAGTACATGGGAAGC27820.10039769323488441No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC250.005960072419.040188
GACCGTC456.657198E-414.809037
CGCACTG456.657198E-414.809035
GTACCGT655.3484167E-513.18166356
CCGTGCA655.3511452E-513.1809479
AGAACCG752.0346123E-411.42410855
TATCGCG752.0346123E-411.42410857
CGCGCGC752.0356396E-411.42348810
GCGCGCC752.0981277E-411.38636111
CGCCCTA1501.8189894E-1011.379580516
GCTAGAC1451.2205419E-911.1372743
CACCGTA803.699741E-410.7101027
TCGCGCG803.7015998E-410.7095199
GCGCCTT909.6949225E-510.53664813
ATCGCGC1002.3449165E-510.4720988
GTAGGAC1701.5625119E-910.058233
ATAGGCG951.6101796E-410.0211485
TATTCCG1054.013106E-59.9734295
GTGTAGG3350.09.9348451
TAGGACT2400.09.9167614
Produced by FastQC (version 0.11.5)