##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294602_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2770980 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.35133923738172 31.0 31.0 34.0 28.0 34.0 2 31.538563612873425 31.0 31.0 34.0 28.0 34.0 3 31.58711105818158 31.0 31.0 34.0 28.0 34.0 4 35.2133028747952 37.0 35.0 37.0 32.0 37.0 5 35.31262621888285 37.0 35.0 37.0 32.0 37.0 6 34.87504456906942 37.0 35.0 37.0 32.0 37.0 7 34.759403532324306 37.0 35.0 37.0 32.0 37.0 8 34.781873200095276 37.0 35.0 37.0 32.0 37.0 9 36.250481057243285 39.0 37.0 39.0 31.0 39.0 10 36.03410309709922 39.0 35.0 39.0 30.0 39.0 11 36.160511804487946 39.0 35.0 39.0 31.0 39.0 12 36.12544117965485 39.0 35.0 39.0 30.0 39.0 13 35.99419194653155 39.0 35.0 39.0 30.0 39.0 14 37.08963363142282 40.0 36.0 41.0 30.0 41.0 15 37.1343726768147 40.0 37.0 41.0 31.0 41.0 16 37.182995907585045 40.0 37.0 41.0 31.0 41.0 17 37.23507279013201 40.0 37.0 41.0 31.0 41.0 18 37.24677442637623 40.0 37.0 41.0 31.0 41.0 19 37.29362752527987 40.0 37.0 41.0 31.0 41.0 20 37.34996463345098 40.0 37.0 41.0 31.0 41.0 21 37.190439844387186 40.0 37.0 41.0 30.0 41.0 22 37.19810391991281 40.0 37.0 41.0 30.0 41.0 23 37.2123154984879 40.0 37.0 41.0 30.0 41.0 24 37.14909346151903 40.0 37.0 41.0 30.0 41.0 25 36.09162642819508 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4413284908925448 1101 2 0.3929856270183727 1101 3 0.35472051617858824 1101 4 0.3159994087161948 1101 5 0.29832982802488317 1101 6 0.3604906690188727 1101 7 0.45914660721269485 1101 8 0.41540929290477635 1101 9 0.5774476603384997 1101 10 0.570645161343954 1101 11 0.5478418878512059 1101 12 0.6101230517983396 1101 13 0.6321970209883574 1101 14 0.8598347212412776 1101 15 0.7646604450186416 1101 16 0.8126427662236253 1101 17 0.836509830017711 1101 18 0.8583213450515785 1101 19 0.9372727192728831 1101 20 0.8285620494617589 1101 21 1.0422133090588375 1101 22 0.9883954666963177 1101 23 1.0227350997728024 1101 24 1.1458772259539103 1101 25 1.0206515516222439 1102 1 0.3523142697667687 1102 2 0.3838638554347007 1102 3 0.3016999401731191 1102 4 0.23927909574313588 1102 5 0.3181264544448581 1102 6 0.32248616988078993 1102 7 0.32126927525285254 1102 8 0.4205228679866977 1102 9 0.4919811626425741 1102 10 0.6908710931052369 1102 11 0.648809627814444 1102 12 0.6214007912727979 1102 13 0.690832416200891 1102 14 0.9194937840333282 1102 15 0.8202127108910346 1102 16 0.8640066930698822 1102 17 0.7933048428565428 1102 18 0.7883779445288752 1102 19 0.8737935405295758 1102 20 0.7484562528009491 1102 21 1.0185146192351127 1102 22 0.9174101242446326 1102 23 0.909417277455077 1102 24 1.093872514617189 1102 25 0.9622975678799293 1103 1 0.1776698669041643 1103 2 0.1934501533099393 1103 3 0.13878801427464893 1103 4 0.14377452406406377 1103 5 0.11432688389417933 1103 6 0.16186989194402202 1103 7 0.1808866439139294 1103 8 0.26097881944460255 1103 9 0.2227534663093067 1103 10 0.33789642659006347 1103 11 0.4217666344007398 1103 12 0.39248607114547696 1103 13 0.48848133107604497 1103 14 0.5898338682164947 1103 15 0.567725365122179 1103 16 0.4489820455290001 1103 17 0.5406014737008604 1103 18 0.5422374841780737 1103 19 0.5443540338434971 1103 20 0.4751415191016264 1103 21 0.7355377856864678 1103 22 0.6640683403647145 1103 23 0.6629392848121114 1103 24 0.866263760237139 1103 25 0.7575517305798769 1104 1 0.18282550720997293 1104 2 0.1901367223249366 1104 3 0.13824559623992627 1104 4 0.13210555591891193 1104 5 0.11734775405425069 1104 6 0.16252112207504155 1104 7 0.24397877162566317 1104 8 0.20497857143514864 1104 9 -0.04423364171564259 1104 10 0.4608528635646607 1104 11 0.4314755476717522 1104 12 0.4588797221033687 1104 13 0.14318564754925234 1104 14 0.39452210924226705 1104 15 0.5052766659194674 1104 16 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2207 3 0.20747649754221698 2207 4 0.18786714304880547 2207 5 0.11857965117581415 2207 6 0.20856059429082308 2207 7 0.13918596212760548 2207 8 0.2738489174823471 2207 9 0.27374175468051476 2207 10 0.5043169411012158 2207 11 0.4449491541686186 2207 12 0.3020038475422595 2207 13 0.3451738522416221 2207 14 0.3347600757106264 2207 15 0.3708238943655573 2207 16 0.4436769903157227 2207 17 0.40061038130065185 2207 18 0.2594588905721764 2207 19 0.5165953900927391 2207 20 0.47324689949643783 2207 21 0.7752145051871153 2207 22 0.5868585808277942 2207 23 0.5348376924533156 2207 24 0.7514850655634717 2207 25 0.7257015467027159 2208 1 0.19963971152417415 2208 2 0.17603427653644488 2208 3 0.10786135946034037 2208 4 0.09746792825802686 2208 5 0.09489943364081199 2208 6 0.2622326723679862 2208 7 0.1988632497659495 2208 8 0.12942852931534077 2208 9 0.20575571001788973 2208 10 0.2760456918388883 2208 11 -0.05275673597913766 2208 12 -0.20515149861389403 2208 13 -0.0912927293709842 2208 14 -0.47775725867663255 2208 15 0.13535174802942862 2208 16 0.23562591858833315 2208 17 0.14016901313213737 2208 18 0.26912637465150624 2208 19 0.4971802985440661 2208 20 0.37946006392350284 2208 21 0.6736991330524873 2208 22 0.6594355416551636 2208 23 0.5891938047123446 2208 24 0.6574307026137589 2208 25 0.6390988516292282 2209 1 -0.002733127163317306 2209 2 -3.1838580759568913E-4 2209 3 0.08314748776371061 2209 4 -0.501270896773903 2209 5 -0.07691570045880525 2209 6 0.05996268022730078 2209 7 0.11879798058430424 2209 8 0.15430357843607823 2209 9 0.24746842737715724 2209 10 0.29520431498458066 2209 11 0.38779949498658084 2209 12 0.38552231512026225 2209 13 0.3436560622588445 2209 14 0.37302468208375217 2209 15 -0.1640483649482647 2209 16 -0.17078065376651352 2209 17 0.1602425100375271 2209 18 -0.22934320733100577 2209 19 0.21919529264957305 2209 20 0.24369801706232153 2209 21 0.5633846989234002 2209 22 0.5898191746249992 2209 23 0.4492933356092905 2209 24 0.6124806215645364 2209 25 0.5274059667682351 2210 1 -0.19361252977852317 2210 2 -0.15347306352810008 2210 3 -0.10738115172118867 2210 4 -0.01911978735829223 2210 5 0.025241371639253884 2210 6 -0.40949950752663256 2210 7 -0.10574712257243135 2210 8 -0.2590453284313199 2210 9 -0.048416895274520755 2210 10 0.26778014340199263 2210 11 0.1833955034785575 2210 12 0.15223701666047873 2210 13 0.26356091521552827 2210 14 0.1810384226163464 2210 15 0.008202791920680852 2210 16 0.19326708491136912 2210 17 -0.8391931092750511 2210 18 -1.5055194026588694 2210 19 -0.4144223924029333 2210 20 -0.16241503120981804 2210 21 -0.28080465152415 2210 22 -0.16898295426198473 2210 23 -0.11592796209992429 2210 24 0.2502153876475006 2210 25 0.22901819778845578 2211 1 -0.2989209796980532 2211 2 -0.22307948124439392 2211 3 -0.1397356283631801 2211 4 -0.13536057985245264 2211 5 -0.13602226180726262 2211 6 -0.009951329862850855 2211 7 -0.1870067091301948 2211 8 -0.07112209317898532 2211 9 -0.06389191173735753 2211 10 -0.08264943789667711 2211 11 -0.378464929136193 2211 12 -0.19199021242881287 2211 13 -0.12489439236951227 2211 14 -0.2298512226684224 2211 15 -0.23001431561372954 2211 16 -0.18544016591447132 2211 17 -0.28374947820893226 2211 18 -0.37976673654566184 2211 19 -0.21260231725556622 2211 20 -0.4031356181297383 2211 21 -0.009184342676526569 2211 22 -0.09649550114741601 2211 23 -0.8136045672004357 2211 24 -0.21900540641411226 2211 25 -0.7865678605147366 2212 1 -0.12948475818180682 2212 2 -0.2663693501365678 2212 3 -0.06779141902543984 2212 4 0.016082168741050396 2212 5 0.0205096611645601 2212 6 -0.05599230823273871 2212 7 -0.12245386180961049 2212 8 -0.33818452455517445 2212 9 -0.11267430816361923 2212 10 -0.35104312851534303 2212 11 -0.08515394814772037 2212 12 -0.13095968369146505 2212 13 -0.1027746922942967 2212 14 -0.14584807276645506 2212 15 0.054590234489587885 2212 16 -0.15794959414721887 2212 17 -0.43110016666089734 2212 18 -0.32889581981701355 2212 19 -0.2461464928443604 2212 20 -0.4272617844732949 2212 21 0.0735117955391047 2212 22 -0.2708478576640232 2212 23 -0.11074806826630379 2212 24 -0.2972797101234903 2212 25 0.005099075358074856 2213 1 -0.17798035359588837 2213 2 -0.1351938471916796 2213 3 -0.06150168490270147 2213 4 0.015864346654446138 2213 5 0.010065286306662813 2213 6 -0.18704490190958722 2213 7 -0.25738420648150395 2213 8 -0.29942271203590565 2213 9 -0.2142178349556545 2213 10 -0.1984404701472755 2213 11 -0.05097610919850837 2213 12 0.08063909967670213 2213 13 0.14298687990862646 2213 14 -0.48302282644220185 2213 15 -0.15084585066816913 2213 16 -0.25641213594971646 2213 17 -0.26080270033957476 2213 18 -0.13474521672008422 2213 19 -0.1566149802512129 2213 20 -0.23798499102154835 2213 21 0.05246388681724312 2213 22 -0.26287825565863443 2213 23 -0.43967778665924584 2213 24 -0.6102483775653198 2213 25 -0.3751716001412291 2214 1 -0.2852719665213037 2214 2 -0.1905562208639111 2214 3 -0.31991369542738823 2214 4 -0.07206780465799056 2214 5 -0.21461040315775648 2214 6 -0.1129123573545101 2214 7 -0.2248247471614846 2214 8 -0.17458823508874843 2214 9 -0.20015625751379673 2214 10 -0.20009369433571322 2214 11 -0.08010816649957775 2214 12 -0.30444232906670265 2214 13 -0.2795242552785666 2214 14 -0.3585529301413217 2214 15 -0.23452857378750736 2214 16 -0.42132067550614494 2214 17 -0.5621996862436021 2214 18 -0.37632632731528304 2214 19 -0.5188075086409256 2214 20 -0.5855730836800674 2214 21 -0.5458141001931409 2214 22 -0.8816822477020665 2214 23 -1.1042498343298845 2214 24 -0.7693391042616824 2214 25 -0.9506197305189303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 6.0 9 23.0 10 59.0 11 188.0 12 458.0 13 1099.0 14 2304.0 15 4075.0 16 6037.0 17 7933.0 18 9771.0 19 10909.0 20 11979.0 21 12977.0 22 14029.0 23 15436.0 24 16966.0 25 18801.0 26 21573.0 27 24341.0 28 28586.0 29 36045.0 30 45638.0 31 59609.0 32 80203.0 33 108909.0 34 150207.0 35 209347.0 36 300906.0 37 480049.0 38 1092515.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.22851473675123 22.30071259027486 12.551081853421046 23.919690819552866 2 14.042288287898144 22.19157121307263 40.79906747793199 22.967073021097228 3 17.68320205847649 28.09661395795952 30.21706880320336 24.00311518036063 4 11.336874501245015 17.998021402205765 39.61494220307228 31.05016189347694 5 12.451861870414268 38.70163040672997 35.32022120889557 13.526286513960192 6 29.49008603902491 39.90688279714 17.611993848013004 12.991037315822092 7 24.37949301062169 34.51437131692702 24.080822301035095 17.025313371416196 8 23.35701159391308 38.554967940677166 21.44662753539015 16.641392930019606 9 23.90398765489573 16.83308811296521 23.166161249263347 36.09676298287571 10 14.308832991340035 31.166747817619033 33.78709033260298 20.73732885843796 11 31.34092831971308 26.870673744792423 22.88122954569829 18.907168389796205 12 19.911705467396956 29.021495492705938 32.6958992086838 18.370899831213304 13 25.927839943933172 24.79748132338787 27.745067717943726 21.529611014735224 14 19.463729820126932 23.842041405234443 29.745639949273787 26.948588825364837 15 22.82024058192359 30.67516965312954 25.11270511030882 21.39188465463805 16 20.234985626013632 32.3175066709299 27.270552129969992 20.17695557308647 17 18.65943408448783 31.392082004230275 30.036279596489184 19.912204314792714 18 18.86509056395414 30.556772538136467 31.354255008174032 19.223881889735363 19 19.557012732049543 29.311538239454922 31.927310390622743 19.204138637872795 20 21.125098972419085 28.617915577699392 30.827752263283596 19.429233186597923 21 21.30740451632298 29.514599755825664 29.947913565317 19.23008216253436 22 19.5512500103757 29.316607582309988 31.373486636276986 19.75865577103732 23 19.29518696661393 29.70617573013985 31.241735763860824 19.756901539385403 24 19.479036109620463 28.91945409907473 31.13931935247434 20.46219043883046 25 20.297379872635002 28.630959813550234 30.08537915374383 20.986281160070934 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1643.0 1 1643.0 2 1925.0 3 2207.0 4 2207.0 5 2207.0 6 4547.5 7 6888.0 8 6888.0 9 6888.0 10 10675.0 11 14462.0 12 14462.0 13 14462.0 14 22607.0 15 30752.0 16 30752.0 17 30752.0 18 48397.0 19 66042.0 20 66042.0 21 66042.0 22 95116.5 23 124191.0 24 124191.0 25 124191.0 26 160185.5 27 196180.0 28 196180.0 29 196180.0 30 231529.5 31 266879.0 32 266879.0 33 266879.0 34 294100.5 35 321322.0 36 321322.0 37 321322.0 38 332892.5 39 344463.0 40 344463.0 41 344463.0 42 336094.5 43 327726.0 44 327726.0 45 327726.0 46 333337.0 47 338948.0 48 338948.0 49 338948.0 50 305841.5 51 272735.0 52 272735.0 53 272735.0 54 232638.0 55 192541.0 56 192541.0 57 192541.0 58 159909.0 59 127277.0 60 127277.0 61 127277.0 62 102377.5 63 77478.0 64 77478.0 65 77478.0 66 57193.5 67 36909.0 68 36909.0 69 36909.0 70 25993.5 71 15078.0 72 15078.0 73 15078.0 74 10126.5 75 5175.0 76 5175.0 77 5175.0 78 3371.0 79 1567.0 80 1567.0 81 1567.0 82 983.0 83 399.0 84 399.0 85 399.0 86 239.0 87 79.0 88 79.0 89 79.0 90 55.5 91 32.0 92 32.0 93 32.0 94 19.5 95 7.0 96 7.0 97 7.0 98 3.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09487618099011902 2 0.0 3 0.1402031050386506 4 0.011945232372662378 5 0.020462074789424682 6 0.017791539455355145 7 0.02544226230431111 8 0.0233491400154458 9 1.0826494597579195E-4 10 0.38585626745772256 11 0.06124187110697299 12 9.743845137821275E-4 13 3.6088315325263985E-5 14 9.382961984568636E-4 15 0.0011548260904084475 16 7.217663065052797E-5 17 3.6088315325263985E-5 18 3.6088315325263985E-4 19 7.217663065052797E-4 20 2.165298919515839E-4 21 3.2479483792737586E-4 22 0.0029953301719969107 23 1.4435326130105594E-4 24 3.6088315325263985E-5 25 0.0016600625049621432 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2770980.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.23655629597782 #Duplication Level Percentage of deduplicated Percentage of total 1 91.40482381811704 72.42603468188165 2 6.760649191881709 10.713811205797839 3 0.9537571164041242 2.267172883399346 4 0.3001971306000347 0.9514634735472263 5 0.13790936328895337 0.5463731513993806 6 0.08010100214155755 0.3808156539332259 7 0.06525156844872888 0.3619216703751989 8 0.03712165814630922 0.2353113884408059 9 0.032158859346701305 0.22933415421353973 >10 0.19428706102621063 2.992990667219547 >50 0.016991577390783538 0.9582062112025616 >100 0.013731884235426397 2.0671947762432406 >500 0.0016013926369872972 0.9415876846020749 >1k 0.0010980978081272913 2.1078773738975958 >5k 9.150815067727428E-5 0.46194787757908506 >10k+ 2.287703766931857E-4 2.357957146267752 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 17467 0.6303546037863861 No Hit GGTATCAACGCAGAGTACTTTTTTT 13659 0.49293029902778074 No Hit GTACATGGGAAGCAGTGGTATCAAC 11475 0.41411341835740423 No Hit CCCATGTACTCTGCGTTGATACCAC 11398 0.41133461807735894 No Hit TATCAACGCAGAGTACTTTTTTTTT 11041 0.3984510895062397 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 6896 0.24886502248302045 No Hit GAGTACATGGGAAGCAGTGGTATCA 5846 0.21097229139149326 No Hit CATGTACTCTGCGTTGATACCACTG 4400 0.15878858743116153 No Hit ATTCCATTCCATTCCATTCCATTCC 3897 0.14063616482255376 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3656 0.13193888082916513 No Hit GCGTTGATACCACTGCTTCCCATGT 3553 0.12822178435066292 No Hit GTATCAACGCAGAGTACATGGGAAG 3386 0.12219503569134385 No Hit GAATGGAATGGAATGGAATGGAATG 3374 0.12176197590744069 No Hit GGTATCAACGCAGAGTACATGGGAA 3197 0.11537434409486898 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2924 0.10552223401107189 No Hit GTGGTATCAACGCAGAGTACATGGG 2816 0.10162469595594338 No Hit TATCAACGCAGAGTACATGGGAAGC 2782 0.10039769323488441 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.6088315325263985E-5 0.0 4 0.0 0.0 0.0 3.6088315325263985E-5 0.0 5 0.0 0.0 0.0 3.6088315325263985E-5 0.0 6 0.0 0.0 0.0 3.6088315325263985E-5 0.0 7 0.0 0.0 0.0 3.6088315325263985E-5 0.0 8 0.0 0.0 0.0 3.6088315325263985E-5 0.0 9 0.0 0.0 0.0 1.0826494597579195E-4 0.0 10 0.0 0.0 0.0 1.0826494597579195E-4 0.0 11 0.0 0.0 0.0 1.0826494597579195E-4 0.0 12 0.0 0.0 0.0 1.0826494597579195E-4 0.0 13 0.0 0.0 0.0 1.0826494597579195E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGGTC 25 0.0059600724 19.04018 8 GACCGTC 45 6.657198E-4 14.80903 7 CGCACTG 45 6.657198E-4 14.80903 5 GTACCGT 65 5.3484167E-5 13.1816635 6 CCGTGCA 65 5.3511452E-5 13.180947 9 AGAACCG 75 2.0346123E-4 11.4241085 5 TATCGCG 75 2.0346123E-4 11.4241085 7 CGCGCGC 75 2.0356396E-4 11.423488 10 GCGCGCC 75 2.0981277E-4 11.386361 11 CGCCCTA 150 1.8189894E-10 11.3795805 16 GCTAGAC 145 1.2205419E-9 11.137274 3 CACCGTA 80 3.699741E-4 10.710102 7 TCGCGCG 80 3.7015998E-4 10.709519 9 GCGCCTT 90 9.6949225E-5 10.536648 13 ATCGCGC 100 2.3449165E-5 10.472098 8 GTAGGAC 170 1.5625119E-9 10.05823 3 ATAGGCG 95 1.6101796E-4 10.021148 5 TATTCCG 105 4.013106E-5 9.973429 5 GTGTAGG 335 0.0 9.934845 1 TAGGACT 240 0.0 9.916761 4 >>END_MODULE