FastQCFastQC Report
Wed 25 May 2016
SRR1294601_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294601_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5109888
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC232840.4556655644898675No Hit
CCCATGTACTCTGCGTTGATACCAC220760.43202512462112674No Hit
GTATCAACGCAGAGTACTTTTTTTT136240.2666203251421557No Hit
GAGTACATGGGAAGCAGTGGTATCA104970.20542524611106938No Hit
GGTATCAACGCAGAGTACTTTTTTT84280.16493512186568474No Hit
CATGTACTCTGCGTTGATACCACTG80140.1568331830364971No Hit
TATCAACGCAGAGTACTTTTTTTTT76990.15066866436211518No Hit
GCGTTGATACCACTGCTTCCCATGT71590.1401009180631748No Hit
GTACTGGTTCACTATCGGTCAGTCA66800.13072693569800356No Hit
GTATCAACGCAGAGTACATGGGAAG66490.13012026878084215No Hit
ACGCAGAGTACATGGGAAGCAGTGG56480.11053079832669523No Hit
TATCAACGCAGAGTACATGGGAAGC51220.10023703063550511No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGT500.001504048413.29567510
CCGGTCG658.0502103E-411.688629
CCCCGTA9750.010.04030215
TAGACAG6550.010.004775
AAATCGC2105.456968E-129.95704217
ATCGCCG2054.1836756E-119.73016219
CCGTACA9900.09.60045417
CCCGTAC10200.09.59753716
GTCTAGA5200.09.4993291
CCGTGCG1106.8665766E-59.4970049
TGTCCCG10350.09.45066211
CGCCCTA10250.09.36526816
GTACTAA3600.09.2354591
TTAGACA5350.09.2310944
GTCCCGC10600.09.14534312
CTTAGAC4500.09.0751813
AATCGCC2201.6734703E-109.07358518
GTCTAAG3700.08.9858521
GTACTGG19900.08.9742411
GTCTTAG4350.08.953391