FastQCFastQC Report
Wed 25 May 2016
SRR1294601_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294601_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5109888
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT318030.6223815473059292No Hit
CCCATGTACTCTGCGTTGATACCAC253080.4952750432103404No Hit
GTACATGGGAAGCAGTGGTATCAAC223710.4377982452844368No Hit
GGTATCAACGCAGAGTACTTTTTTT216540.42376662658751035No Hit
TATCAACGCAGAGTACTTTTTTTTT192020.3757812304300994No Hit
ACGCAGAGTACTTTTTTTTTTTTTT121040.23687407630069388No Hit
GAGTACATGGGAAGCAGTGGTATCA107060.20951535532677035No Hit
CATGTACTCTGCGTTGATACCACTG92120.1802779238997019No Hit
GCGTTGATACCACTGCTTCCCATGT77680.15201898750031312No Hit
ACGCAGAGTACATGGGAAGCAGTGG65760.12869166604042984No Hit
GTATCAACGCAGAGTACATGGGAAG64400.12603015956514116No Hit
GTACTTTTTTTTTTTTTTTTTTTTT61910.1211572543272964No Hit
GTACTGGTTCACTATCGGTCAGTCA61270.11990478069186644No Hit
GAGTACTTTTTTTTTTTTTTTTTTT57170.11188112146489317No Hit
TATCAACGCAGAGTACATGGGAAGC53760.10520778537611783No Hit
ACTCTGCGTTGATACCACTGCTTCC53230.10417058064677738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCC2450.012.38628918
ACCGACC600.005792320711.1054158
CCCCGTA8700.010.79149815
CCGTACA8750.010.72962317
CCCGTAC8900.010.54888916
AAATCGC3100.010.40098617
GTATTAG6150.010.2048091
GTCCTAG5000.010.0795691
GTCTACG1252.5422487E-69.8893881
GAAATCG3200.09.77969916
CGAGCGG1106.698401E-59.51855410
GCCCCGT8600.09.48334514
ATCGCCG3250.09.33781219
TTAGACC2054.0927262E-109.2867584
TAGACAG7150.09.1860975
ACGGAGT1252.692936E-59.138178
ATACCGA1252.692936E-59.138176
CTTAGAC3550.09.1000043
GTACTGG20600.09.0474231
TAGACCG950.00179218919.0179315
Produced by FastQC (version 0.11.5)