##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294601_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5109888 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.25205816644122 31.0 31.0 34.0 28.0 34.0 2 31.430426655143908 31.0 31.0 34.0 28.0 34.0 3 31.492076342964857 31.0 31.0 34.0 28.0 34.0 4 35.13384148537111 37.0 35.0 37.0 32.0 37.0 5 35.23753710453145 37.0 35.0 37.0 32.0 37.0 6 34.75052134215075 37.0 35.0 37.0 32.0 37.0 7 34.61963354187019 37.0 35.0 37.0 31.0 37.0 8 34.63423699306129 37.0 35.0 37.0 31.0 37.0 9 36.08527955994339 39.0 35.0 39.0 30.0 39.0 10 35.84987909715438 39.0 35.0 39.0 30.0 39.0 11 35.98815590478696 39.0 35.0 39.0 30.0 39.0 12 35.94785208599484 39.0 35.0 39.0 30.0 39.0 13 35.8082329788833 39.0 35.0 39.0 30.0 39.0 14 36.875553828185666 40.0 36.0 41.0 30.0 41.0 15 36.91311296842514 40.0 36.0 41.0 30.0 41.0 16 36.94983158143584 40.0 36.0 41.0 30.0 41.0 17 37.00294213884923 40.0 36.0 41.0 30.0 41.0 18 37.005020462288016 40.0 36.0 41.0 30.0 41.0 19 37.005963927193704 40.0 36.0 41.0 30.0 41.0 20 37.08451026715262 40.0 36.0 41.0 30.0 41.0 21 36.91470458843716 40.0 36.0 41.0 30.0 41.0 22 36.91855418357506 40.0 36.0 41.0 30.0 41.0 23 36.921794959106734 40.0 36.0 41.0 30.0 41.0 24 36.85274041231432 40.0 36.0 41.0 30.0 41.0 25 35.70227057814183 39.0 34.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5187391717424958 1101 2 0.49930918537426194 1101 3 0.4471810415603841 1101 4 0.36721049265463535 1101 5 0.37612589093077275 1101 6 0.4637699314074979 1101 7 0.5541725044076813 1101 8 0.5606094100678902 1101 9 0.7217471733875911 1101 10 0.7617650255977324 1101 11 0.7863579270990186 1101 12 0.8421308275078516 1101 13 0.7939787291065628 1101 14 0.9975064945751981 1101 15 0.9766321661186339 1101 16 1.0213475689047158 1101 17 1.0517831295166857 1101 18 1.032894120721572 1101 19 1.1492985560741502 1101 20 1.0656817022233582 1101 21 1.2919620090200823 1101 22 1.2255969166835925 1101 23 1.266507916422249 1101 24 1.2958340642855504 1101 25 1.2977903726826625 1102 1 0.39993723834974304 1102 2 0.42374894604557767 1102 3 0.3588839995883859 1102 4 0.3084270281346093 1102 5 0.3652426822417283 1102 6 0.4072781378574106 1102 7 0.43045039509674154 1102 8 0.47767883832177915 1102 9 0.5784575597618726 1102 10 0.718198271745635 1102 11 0.6408059869138754 1102 12 0.7268511875962318 1102 13 0.7174171372137508 1102 14 0.9027664206997059 1102 15 0.8804102691502322 1102 16 0.8584153275591433 1102 17 0.8069507991286642 1102 18 0.7713811481994242 1102 19 0.9544469884376738 1102 20 0.8474817867146953 1102 21 1.158483070700207 1102 22 1.1080419546776952 1102 23 0.9527327201907454 1102 24 1.0542976072356112 1102 25 1.0281201147201173 1103 1 0.3065019855384925 1103 2 0.26312478758907787 1103 3 0.20123741132454853 1103 4 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0.2887298809333423 2207 3 0.3112009537629419 2207 4 0.29260262392602954 2207 5 0.26427769290707204 2207 6 0.4318311264628818 2207 7 0.36203073215700954 2207 8 0.3916352282164013 2207 9 0.48305436713928174 2207 10 0.7246190530046022 2207 11 0.5352302992118183 2207 12 0.431197697678094 2207 13 0.6016343878722381 2207 14 0.6266873290312631 2207 15 0.6046472749447247 2207 16 0.6406771065846328 2207 17 0.7223059529826372 2207 18 0.6668226848623391 2207 19 0.8125906034529677 2207 20 0.6476058884090463 2207 21 0.9778111442441713 2207 22 0.891120508226166 2207 23 0.7825475558686392 2207 24 0.8673977662417869 2207 25 0.8970601326166872 2208 1 0.10847295042161775 2208 2 0.15885690851222733 2208 3 0.17364185151424394 2208 4 0.15895878590360013 2208 5 0.10068028718003319 2208 6 0.21664967300905857 2208 7 0.16405828320708338 2208 8 0.10708073160925835 2208 9 0.279777040093407 2208 10 0.3209259824573252 2208 11 -0.04265984752228036 2208 12 -0.18262214797454135 2208 13 0.029554151184164823 2208 14 -0.3930322585568291 2208 15 0.08864137846154563 2208 16 0.2524084697791693 2208 17 0.17349720814530656 2208 18 0.33819051034282666 2208 19 0.48193955595297666 2208 20 0.3884164042594307 2208 21 0.6436019183825152 2208 22 0.7001144980544254 2208 23 0.6472088053744258 2208 24 0.7013870967259805 2208 25 0.6437105987105411 2209 1 0.05902011982723465 2209 2 0.019901796772565206 2209 3 0.10415400884757631 2209 4 -0.5126772495309382 2209 5 -0.019460024541153587 2209 6 0.1499965488267776 2209 7 0.1642306867323171 2209 8 0.1478993324795539 2209 9 0.2520761081330676 2209 10 0.3254732344950497 2209 11 0.377193809213054 2209 12 0.332045342036146 2209 13 0.40054451913984224 2209 14 0.4480160910957167 2209 15 -0.20107836922758082 2209 16 -0.18750231118544036 2209 17 0.11668747435315652 2209 18 -0.17331549472609709 2209 19 0.23553230769861955 2209 20 0.23152632556492136 2209 21 0.5633680740197491 2209 22 0.5525252785499575 2209 23 0.5613039479536539 2209 24 0.6447124844429979 2209 25 0.6608481676986884 2210 1 -0.18731725123059917 2210 2 -0.1862225586770947 2210 3 -0.14065983152522676 2210 4 -0.036397011723451556 2210 5 -0.08079073503776613 2210 6 -0.6149987391603631 2210 7 -0.21046300222166536 2210 8 -0.3033567863776696 2210 9 -0.09313676171160523 2210 10 0.06434845837041792 2210 11 0.03649401903697225 2210 12 -0.03153641006529995 2210 13 0.07959266233186213 2210 14 0.02275013312666374 2210 15 -0.09688903558127038 2210 16 0.09554401154884573 2210 17 -0.9725513954706742 2210 18 -1.6165752016311927 2210 19 -0.39753175588870704 2210 20 -0.2778303695037252 2210 21 -0.5192676714502866 2210 22 -0.4431970158491083 2210 23 -0.27207847671418506 2210 24 0.128971876710402 2210 25 0.21318508137633074 2211 1 -0.21734145538579952 2211 2 -0.2182827478303686 2211 3 -0.11984171696011359 2211 4 -0.05350553020456772 2211 5 -0.11139637755929499 2211 6 -0.013440551815072865 2211 7 -0.1215517648450728 2211 8 -0.11535821875067853 2211 9 -0.023166555101433062 2211 10 -0.011468729271030043 2211 11 -0.30239951064582016 2211 12 -0.17054653555083377 2211 13 -0.04403358238932498 2211 14 -0.17945347625518338 2211 15 -0.2624936306576018 2211 16 -0.21658114065834155 2211 17 -0.3528701245980699 2211 18 -0.37316414088299865 2211 19 -0.21091906882534772 2211 20 -0.279166452054028 2211 21 -0.001991417424761721 2211 22 -0.07533283128626778 2211 23 -0.7294995469118106 2211 24 -0.1970802889073937 2211 25 -0.7910812734633055 2212 1 -0.20501229188996817 2212 2 -0.30458376207812776 2212 3 -0.15601674182314085 2212 4 -0.13871642071335089 2212 5 -0.07010920638281704 2212 6 -0.14844334582705443 2212 7 -0.185579169100059 2212 8 -0.40060489430459967 2212 9 -0.1607531964567741 2212 10 -0.46146601728344905 2212 11 -0.23090668140584825 2212 12 -0.3906350006798718 2212 13 -0.26924923774669907 2212 14 -0.34343464010122915 2212 15 -0.2154094433919127 2212 16 -0.3811572981428597 2212 17 -0.5646343529720639 2212 18 -0.37278457778069196 2212 19 -0.37611801507291887 2212 20 -0.6848237010659659 2212 21 -0.07251212919477723 2212 22 -0.5131932917835371 2212 23 -0.3568505001626221 2212 24 -0.4911699283830657 2212 25 -0.2051619213470346 2213 1 -0.18883708476508332 2213 2 -0.2082658296052955 2213 3 -0.1783149778919224 2213 4 -0.062166775454620904 2213 5 -0.08946461460551092 2213 6 -0.2318978783931982 2213 7 -0.2821722537681879 2213 8 -0.2907916717091297 2213 9 -0.31548979091627416 2213 10 -0.2874845345300514 2213 11 -0.05540542791347747 2213 12 0.03763349087553536 2213 13 0.091776142365525 2213 14 -0.6223645095030435 2213 15 -0.18825454386923468 2213 16 -0.331701565901092 2213 17 -0.19338944984016138 2213 18 -0.24170973901500048 2213 19 -0.2007988748678855 2213 20 -0.3413714996890107 2213 21 -0.08507460747028972 2213 22 -0.3865993556691336 2213 23 -0.46801822366016665 2213 24 -0.7556173980023999 2213 25 -0.4528941037614871 2214 1 -0.20055631907662885 2214 2 -0.04165301231706309 2214 3 -0.1134933684559023 2214 4 -0.08578814260393841 2214 5 -0.10507601098025532 2214 6 -0.03486399683768582 2214 7 -0.09307106025293166 2214 8 -0.08456185487038681 2214 9 -0.23541552848115543 2214 10 -0.07176498966736489 2214 11 -0.03061816846339127 2214 12 -0.24562702152910987 2214 13 -0.2879518744837739 2214 14 -0.16528880769178755 2214 15 -0.08751547002647442 2214 16 -0.3179234864089935 2214 17 -0.4661151775582937 2214 18 -0.29862931313387264 2214 19 -0.4134453599710213 2214 20 -0.4661576229763895 2214 21 -0.5426184793281763 2214 22 -0.7399914759546746 2214 23 -0.9975232094025088 2214 24 -0.5510896712533153 2214 25 -0.867152969440312 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 13.0 9 55.0 10 153.0 11 357.0 12 984.0 13 2421.0 14 5138.0 15 9042.0 16 13437.0 17 17184.0 18 20603.0 19 22857.0 20 24484.0 21 26274.0 22 28660.0 23 31083.0 24 34501.0 25 38819.0 26 43641.0 27 49438.0 28 57817.0 29 71701.0 30 90638.0 31 117801.0 32 156566.0 33 209621.0 34 284318.0 35 395255.0 36 559561.0 37 875786.0 38 1921680.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30065340017053 20.393090024349963 11.86701797266716 25.439238602812342 2 15.000798451942588 21.424187770847425 38.62804820771022 24.946965569499763 3 18.244717165850304 26.722745952877563 29.4018065768238 25.630730304448328 4 12.237730552755105 17.33075998517996 37.518958087580344 32.912551374484586 5 13.37975194211787 37.83478741377255 34.48309138460521 14.302369259504374 6 31.306780938534835 37.84261351881765 17.09468707448242 13.755918468165095 7 26.228767908399192 33.49712338141269 22.58472479513684 17.689383915051277 8 25.182656986552665 36.35842441797078 20.735685793380288 17.723232802096266 9 25.347679397559236 16.11394005338677 21.412224944617094 37.126155604436896 10 15.574110766093344 29.588686648008856 32.566693156958415 22.270509428939384 11 32.743231125845064 25.45875678267989 22.42411887580967 19.373893215665376 12 21.657682636999276 27.349325062702945 31.025138322222674 19.967853978075105 13 27.510094154705545 22.941050762756444 26.61164002029007 22.93721506224794 14 20.951839326255868 22.77636079300439 27.995415897153585 28.276383983586157 15 24.131923005642655 28.92284653752601 24.207189712219332 22.738040744612 16 21.787771924193912 29.952151518235258 26.20071312829264 22.059363429278182 17 20.751359080934666 29.276009430345525 28.25671096053592 21.71592052818389 18 21.250478975378222 28.20025026096575 29.389674797734422 21.159595965921614 19 21.498717476778666 28.007285538183226 29.85978646335733 20.634210521680778 20 22.783638654033084 26.838651371570293 29.03089763679058 21.346812337606043 21 22.543210973427705 27.821582971468363 28.59119144103638 21.044014614067557 22 21.81702999842848 27.481940724124566 28.77651745180393 21.924511825643027 23 21.46854720658802 27.75317619983248 29.118139760620604 21.660136832958894 24 21.622219825996154 27.5657172066623 28.847937341862938 21.964125625478605 25 22.085317350428873 26.944066031637682 28.37126670297603 22.59934991495742 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3030.0 1 3030.0 2 3274.0 3 3518.0 4 3518.0 5 3518.0 6 7498.0 7 11478.0 8 11478.0 9 11478.0 10 15280.0 11 19082.0 12 19082.0 13 19082.0 14 28065.5 15 37049.0 16 37049.0 17 37049.0 18 60409.5 19 83770.0 20 83770.0 21 83770.0 22 124663.0 23 165556.0 24 165556.0 25 165556.0 26 221223.5 27 276891.0 28 276891.0 29 276891.0 30 334848.5 31 392806.0 32 392806.0 33 392806.0 34 441663.5 35 490521.0 36 490521.0 37 490521.0 38 518587.0 39 546653.0 40 546653.0 41 546653.0 42 560815.0 43 574977.0 44 574977.0 45 574977.0 46 621625.0 47 668273.0 48 668273.0 49 668273.0 50 626365.0 51 584457.0 52 584457.0 53 584457.0 54 517904.5 55 451352.0 56 451352.0 57 451352.0 58 389858.0 59 328364.0 60 328364.0 61 328364.0 62 281397.0 63 234430.0 64 234430.0 65 234430.0 66 181822.5 67 129215.0 68 129215.0 69 129215.0 70 97691.5 71 66168.0 72 66168.0 73 66168.0 74 47092.5 75 28017.0 76 28017.0 77 28017.0 78 18890.5 79 9764.0 80 9764.0 81 9764.0 82 6522.0 83 3280.0 84 3280.0 85 3280.0 86 2060.0 87 840.0 88 840.0 89 840.0 90 571.0 91 302.0 92 302.0 93 302.0 94 190.0 95 78.0 96 78.0 97 78.0 98 47.5 99 17.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09289831792790762 2 0.0 3 0.13221424814007665 4 0.011565811227173668 5 0.022720654542721874 6 0.013092263470353948 7 0.0217617294155958 8 0.019472051050825383 9 1.565592044287468E-4 10 0.3908696237569199 11 0.06487422033516194 12 0.0015655920442874678 13 0.0 14 0.001076344530447634 15 0.0011546241326620076 16 1.565592044287468E-4 17 1.956990055359335E-5 18 4.305378121790536E-4 19 7.632261215901405E-4 20 2.935485083039002E-4 21 3.326883094110869E-4 22 0.003659571403521956 23 1.565592044287468E-4 24 1.956990055359335E-5 25 0.001859140552591368 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5109888.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.09711819632861 #Duplication Level Percentage of deduplicated Percentage of total 1 75.84197604113481 43.303582702637975 2 14.582339355677782 16.652191075402175 3 4.702171129429988 8.05441262269284 4 1.8923837071514251 4.3219862480012585 5 0.9496491181102586 2.711111397089034 6 0.5517102331793197 1.890063863637818 7 0.32299503923477607 1.2909460152411067 8 0.21507400900189513 0.9824084890351562 9 0.15363214370926637 0.7894757401310993 >10 0.7163920357654368 7.043338209100722 >50 0.03954016831582782 1.5586808441909863 >100 0.026129976388663212 2.9667062501877104 >500 0.0026928145923605347 1.0775642626257285 >1k 0.0027273337119381362 3.2792284988691156 >5k 3.452321154342222E-4 1.2566049181235173 >10k+ 2.4166248080395553E-4 2.821698863033855 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 31803 0.6223815473059292 No Hit CCCATGTACTCTGCGTTGATACCAC 25308 0.4952750432103404 No Hit GTACATGGGAAGCAGTGGTATCAAC 22371 0.4377982452844368 No Hit GGTATCAACGCAGAGTACTTTTTTT 21654 0.42376662658751035 No Hit TATCAACGCAGAGTACTTTTTTTTT 19202 0.3757812304300994 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 12104 0.23687407630069388 No Hit GAGTACATGGGAAGCAGTGGTATCA 10706 0.20951535532677035 No Hit CATGTACTCTGCGTTGATACCACTG 9212 0.1802779238997019 No Hit GCGTTGATACCACTGCTTCCCATGT 7768 0.15201898750031312 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6576 0.12869166604042984 No Hit GTATCAACGCAGAGTACATGGGAAG 6440 0.12603015956514116 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 6191 0.1211572543272964 No Hit GTACTGGTTCACTATCGGTCAGTCA 6127 0.11990478069186644 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 5717 0.11188112146489317 No Hit TATCAACGCAGAGTACATGGGAAGC 5376 0.10520778537611783 No Hit ACTCTGCGTTGATACCACTGCTTCC 5323 0.10417058064677738 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 1.565592044287468E-4 0.0 0.0 1.956990055359335E-5 0.0 10 1.565592044287468E-4 0.0 0.0 1.956990055359335E-5 0.0 11 1.565592044287468E-4 0.0 0.0 1.956990055359335E-5 0.0 12 1.565592044287468E-4 0.0 0.0 3.91398011071867E-5 0.0 13 1.565592044287468E-4 0.0 0.0 3.91398011071867E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCGCC 245 0.0 12.386289 18 ACCGACC 60 0.0057923207 11.105415 8 CCCCGTA 870 0.0 10.791498 15 CCGTACA 875 0.0 10.729623 17 CCCGTAC 890 0.0 10.548889 16 AAATCGC 310 0.0 10.400986 17 GTATTAG 615 0.0 10.204809 1 GTCCTAG 500 0.0 10.079569 1 GTCTACG 125 2.5422487E-6 9.889388 1 GAAATCG 320 0.0 9.779699 16 CGAGCGG 110 6.698401E-5 9.518554 10 GCCCCGT 860 0.0 9.483345 14 ATCGCCG 325 0.0 9.337812 19 TTAGACC 205 4.0927262E-10 9.286758 4 TAGACAG 715 0.0 9.186097 5 ACGGAGT 125 2.692936E-5 9.13817 8 ATACCGA 125 2.692936E-5 9.13817 6 CTTAGAC 355 0.0 9.100004 3 GTACTGG 2060 0.0 9.047423 1 TAGACCG 95 0.0017921891 9.017931 5 >>END_MODULE