FastQCFastQC Report
Wed 25 May 2016
SRR1294600_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294600_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3989273
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC166410.4171436750505669No Hit
CCCATGTACTCTGCGTTGATACCAC157240.3941570306168567No Hit
GTATCAACGCAGAGTACTTTTTTTT139760.35033952301584775No Hit
GGTATCAACGCAGAGTACTTTTTTT94600.2371359393052318No Hit
TATCAACGCAGAGTACTTTTTTTTT85260.21372315206304504No Hit
GAGTACATGGGAAGCAGTGGTATCA73470.18416889493398922No Hit
CATGTACTCTGCGTTGATACCACTG59040.14799689066153157No Hit
ATTCCATTCCATTCCATTCCATTCC51040.1279431114391018No Hit
GCGTTGATACCACTGCTTCCCATGT46880.11751514624343834No Hit
GTATCAACGCAGAGTACATGGGAAG42480.10648556767110198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCGG604.104064E-412.6631737
TACACCG1157.10952E-811.5623175
AAATCGC1803.929017E-1010.031301517
TCTAGAC2850.09.9978683
TATACTG10100.09.9677965
AATACCG1054.1199302E-59.9498845
CGCCCTA6800.09.92247916
TGTCCCG7100.09.89830411
AGCACCG1554.0852683E-89.8039915
AATCGCC1752.5538611E-99.7765818
GTTCTAG3700.09.7577361
GAAATCG1901.0477379E-99.50321916
GCTACAC2401.8189894E-129.4979743
ACCGCTC900.00111713479.4973788
GTCCCGC7400.09.37456212
GTATTAG5000.09.3109351
TCCCGCC7650.09.192656513
CCGTACA5600.09.16393317
CCCGCCC7500.09.1230914
CTAGGAC2304.1836756E-119.0850193
Produced by FastQC (version 0.11.5)