##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294600_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3989273 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.996376532766746 31.0 30.0 33.0 25.0 34.0 2 30.170843158640686 31.0 30.0 34.0 25.0 34.0 3 30.0993481769736 31.0 30.0 34.0 25.0 34.0 4 33.72073182256516 35.0 35.0 37.0 28.0 37.0 5 34.096605822664934 35.0 35.0 37.0 29.0 37.0 6 34.17125827187059 37.0 35.0 37.0 30.0 37.0 7 34.10203939414525 37.0 35.0 37.0 29.0 37.0 8 33.96982207033712 37.0 35.0 37.0 28.0 37.0 9 35.0580424052202 39.0 35.0 39.0 27.0 39.0 10 35.222334495533396 39.0 35.0 39.0 27.0 39.0 11 35.42971238117823 39.0 35.0 39.0 28.0 39.0 12 35.2306711022284 39.0 35.0 39.0 27.0 39.0 13 35.065362786653104 39.0 35.0 39.0 27.0 39.0 14 35.98027134267321 39.0 35.0 41.0 27.0 41.0 15 35.95743936301176 39.0 35.0 41.0 27.0 41.0 16 36.0782104909842 39.0 35.0 41.0 27.0 41.0 17 36.0887469972599 39.0 35.0 41.0 27.0 41.0 18 35.98163775705498 39.0 35.0 41.0 27.0 41.0 19 36.26477029774598 39.0 36.0 41.0 27.0 41.0 20 36.375712567177025 39.0 36.0 41.0 27.0 41.0 21 36.42198515869934 39.0 36.0 41.0 27.0 41.0 22 36.43508629266535 39.0 36.0 41.0 27.0 41.0 23 36.42320593250951 39.0 36.0 41.0 27.0 41.0 24 36.438257547177145 39.0 36.0 41.0 27.0 41.0 25 35.292839823195855 39.0 33.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.005684992711263 1101 2 0.9394996669710345 1101 3 1.243091624496028 1101 4 1.0984032271815138 1101 5 0.8421407984290212 1101 6 0.7319057217489728 1101 7 0.8016089209672046 1101 8 0.9739320328881291 1101 9 1.1798238409564306 1101 10 1.062241370504168 1101 11 0.9344778756874348 1101 12 1.16898803029801 1101 13 1.192585311962155 1101 14 1.2611038296886647 1101 15 1.3710773019174312 1101 16 1.2962252269496304 1101 17 1.2938333705646556 1101 18 1.3251182970115565 1101 19 1.2311299926842452 1101 20 1.125224686309238 1101 21 0.9849418414767186 1101 22 0.991197015977157 1101 23 0.967361426362956 1101 24 1.0455817264295177 1101 25 1.015822221550664 1102 1 0.9717131012820985 1102 2 0.9568368994228891 1102 3 1.2289884827676687 1102 4 1.0622909505900253 1102 5 0.8827594009977133 1102 6 0.8426946596662575 1102 7 0.770345950109224 1102 8 0.8060109182320829 1102 9 1.1519508927103317 1102 10 1.033027267221648 1102 11 0.7868538834368621 1102 12 1.0531671034642045 1102 13 1.0896420359704564 1102 14 1.3544669742892665 1102 15 1.4566097089989185 1102 16 1.2843750370154368 1102 17 1.3308789857458763 1102 18 1.4691531103015976 1102 19 1.1002323177941378 1102 20 0.38695688895452207 1102 21 0.72477740549256 1102 22 0.7731443271904581 1102 23 0.8502270813613606 1102 24 0.9244030080699588 1102 25 1.044688839595679 1103 1 0.7561964730396724 1103 2 0.8107503607445103 1103 3 1.0448240408906209 1103 4 0.8708685770994578 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0.2929199186943521 2207 7 0.22454038932177411 2207 8 0.21731877960393575 2207 9 -1.4468100012140752 2207 10 -0.306477011182551 2207 11 0.05441861200946363 2207 12 0.3768650855008815 2207 13 0.49891237352254336 2207 14 0.7188283011173908 2207 15 0.7377865777433996 2207 16 0.7402037999115905 2207 17 0.6250210470391551 2207 18 0.7034145334808173 2207 19 -0.373162509593584 2207 20 0.18624100131552979 2207 21 -0.4078428989584211 2207 22 0.180643005290392 2207 23 -0.5351862173326367 2207 24 -1.6685997287497116 2207 25 -0.2715694921680907 2208 1 0.45075064588119673 2208 2 0.14305924342529508 2208 3 0.6413403994387821 2208 4 0.5322906772354585 2208 5 0.3896836023793213 2208 6 0.26490289596956984 2208 7 0.35641124540374847 2208 8 0.4780559228075205 2208 9 -0.18477416335989716 2208 10 -0.0049513147970898785 2208 11 0.2210822360330127 2208 12 0.2479567050564313 2208 13 0.4429233972241349 2208 14 0.7783059658452132 2208 15 0.6245679976951877 2208 16 0.47312200570853236 2208 17 0.4078670219276077 2208 18 0.6691696417445314 2208 19 -1.4104836368706586 2208 20 -0.1593366834382266 2208 21 -0.19411178567730047 2208 22 0.15019679694898258 2208 23 0.28636214715520225 2208 24 0.5334901600147504 2208 25 0.5113162169668684 2209 1 0.1530478454920079 2209 2 -1.474320110984614 2209 3 -0.2259775933725514 2209 4 0.10011683952079409 2209 5 0.08461906196851743 2209 6 0.11098488142778962 2209 7 0.09359321735384896 2209 8 0.3547806547986525 2209 9 -1.9200753380766216 2209 10 -0.3185273145934886 2209 11 -0.015426226606280125 2209 12 0.30532582315260726 2209 13 0.4155809625525251 2209 14 0.646705648012933 2209 15 0.7405244726836386 2209 16 0.685095984475339 2209 17 0.4440507881079796 2209 18 0.638021686755927 2209 19 0.3657257188704861 2209 20 0.4180432900936921 2209 21 0.34500424512903294 2209 22 0.38658632943110405 2209 23 0.4179665639177941 2209 24 0.5802630538252913 2209 25 0.5524864848191129 2210 1 0.12003605226386682 2210 2 0.12429408198054048 2210 3 0.18737828152178437 2210 4 0.28528343574507886 2210 5 0.14981495048171212 2210 6 0.0854627272998627 2210 7 0.13960970087033076 2210 8 0.14365897733082278 2210 9 0.279425548323303 2210 10 -1.5269985975840044 2210 11 -0.402380151420779 2210 12 -2.301191857884305 2210 13 -0.7128560384806306 2210 14 -0.4394945433555293 2210 15 -0.6814217034692689 2210 16 -0.1066403907946949 2210 17 0.028084142574023474 2210 18 0.297802610959927 2210 19 0.25909283519433757 2210 20 0.2633929190971642 2210 21 0.2911128907569136 2210 22 0.176541099554143 2210 23 0.10257989396382072 2210 24 0.18513781314716482 2210 25 0.0983253948407139 2211 1 -0.24052241829568644 2211 2 -0.013640486621980585 2211 3 -1.3463849087977628 2211 4 -0.5558474492370209 2211 5 -0.37162492072192066 2211 6 -0.370616893405888 2211 7 -0.3958951959329795 2211 8 0.016723511535708724 2211 9 0.2061373336798411 2211 10 0.1939990246097949 2211 11 -0.17978179304244435 2211 12 -0.18568302286310967 2211 13 -0.33466854338134056 2211 14 -0.7529047835036167 2211 15 -0.32546044675923014 2211 16 -0.1059627532161258 2211 17 -0.17168575721320423 2211 18 0.04437307430059434 2211 19 4.76439299056608E-4 2211 20 -0.08017979649063989 2211 21 -0.14705931034130515 2211 22 -0.11970989098833229 2211 23 -0.5302725582575576 2211 24 -0.27921731249887927 2211 25 -0.07622240345092024 2212 1 -0.30401137288508195 2212 2 -0.2586839236337788 2212 3 -2.3254443888417313 2212 4 -1.3353481280891586 2212 5 -0.7084664449072591 2212 6 -0.3898254616036212 2212 7 -0.296370604292548 2212 8 -0.3177058019956789 2212 9 -1.0348418716789283 2212 10 -0.1390099925493189 2212 11 -0.2679717928638041 2212 12 0.10523324634463904 2212 13 -0.35061761114903334 2212 14 0.052620323449538375 2212 15 0.11179505977686688 2212 16 -1.1665482009434598 2212 17 -1.2452066322428195 2212 18 -0.17345783743242293 2212 19 -0.09843302786209307 2212 20 -0.1723741815886939 2212 21 -0.06200815211307997 2212 22 -0.28155274650555384 2212 23 -0.02322526185785989 2212 24 -0.1809695791443673 2212 25 -0.0920795406762096 2213 1 -3.0190588311019866 2213 2 -1.321690189466672 2213 3 -2.4818994698777033 2213 4 -0.9906742290981541 2213 5 -0.7919262625559895 2213 6 -0.5255986806889013 2213 7 -0.3425087959396649 2213 8 -0.11573305079596707 2213 9 -0.2554257612589339 2213 10 -0.1538603616131553 2213 11 -0.4728107947007629 2213 12 -0.17411518285194916 2213 13 -0.6199839450243516 2213 14 -0.2126978328388347 2213 15 -0.3949667325363677 2213 16 -1.559090928180261 2213 17 -1.0469370542643048 2213 18 -0.2740251616530216 2213 19 -0.14476578738025125 2213 20 -0.12742492641295655 2213 21 0.026596082245987418 2213 22 -0.03205381107665772 2213 23 -0.015146448369605992 2213 24 0.1221997617799957 2213 25 0.05780352369355768 2214 1 -1.03592375083608 2214 2 -0.8214864498019736 2214 3 -6.459499972281197 2214 4 -2.262665451507207 2214 5 -1.4652299996160565 2214 6 -0.8953922134015642 2214 7 -0.7347712829527069 2214 8 -0.6234237528585425 2214 9 -0.3781558655308075 2214 10 -0.33352535601320454 2214 11 -1.078289734849342 2214 12 -0.7110049044173863 2214 13 -5.337214272136489 2214 14 -3.977457165359912 2214 15 -2.3745309423650696 2214 16 -2.9969279740708856 2214 17 -3.759355225585054 2214 18 -1.5366666435118006 2214 19 -0.9852566581647295 2214 20 -0.5745396515235868 2214 21 -0.5788005951189419 2214 22 -0.5944266184876881 2214 23 -0.5737254365590729 2214 24 -0.48005855783081586 2214 25 -0.23230066859733256 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1563.0 3 183.0 4 208.0 5 449.0 6 72.0 7 64.0 8 123.0 9 271.0 10 820.0 11 2185.0 12 4774.0 13 8675.0 14 13926.0 15 20363.0 16 25934.0 17 28947.0 18 30158.0 19 30326.0 20 29676.0 21 29626.0 22 29819.0 23 30161.0 24 31981.0 25 34433.0 26 37490.0 27 42910.0 28 49730.0 29 60958.0 30 75782.0 31 95507.0 32 126054.0 33 168522.0 34 228604.0 35 314857.0 36 443921.0 37 676042.0 38 1314159.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03867029004599 21.419466379606696 12.770505090044992 22.771358240302316 2 14.789132807117383 22.45521349818903 40.26454929581351 22.491104398880083 3 17.841673712537016 28.546773250557 30.089925454649887 23.521627582256095 4 11.786050902473292 17.915209695938504 38.58669617476526 31.712043226822946 5 12.725583261751447 39.040723301001314 34.703977026916874 13.529716410330364 6 29.755883303198566 39.49022409430142 17.69365684895358 13.060235753546436 7 24.499816514313764 34.674806424482284 24.072218360759194 16.753158700444757 8 23.400092655554854 38.091691876887005 21.795751940963314 16.712463526594828 9 24.437045210717205 17.25498350849935 22.14157982781815 36.1663914529653 10 14.966924561109545 30.798310558956356 33.10242901046595 21.132335869468154 11 31.414104499643454 27.410670189601905 22.80633195270324 18.3688933580514 12 20.000943115345766 29.023421617457867 32.076832797939794 18.898802469256573 13 26.096999428364605 25.21378637089781 26.893927897688553 21.79528630304903 14 19.81391974800002 24.220131754169586 28.535917346495204 27.43003115133519 15 23.277652997592412 30.77332721946658 24.18851211794813 21.76050766499288 16 21.08619258196485 31.80597325654603 26.266376038748156 20.841458122740967 17 19.707333444872177 30.77927775504916 28.974982272096415 20.538406527982247 18 20.213761016027558 29.92995631495208 29.742985715626958 20.113296953393412 19 20.566682227646684 29.33936239783247 29.899356999568795 20.194598374952054 20 22.287798350270837 28.398744329604696 28.687127120950457 20.626330199174006 21 22.297851826063226 29.408692825474287 28.252134278178044 20.041321070284447 22 21.13344989075877 28.94548975440163 29.048936559816784 20.872123795022816 23 20.79688660124817 29.38632532659081 29.076891818674373 20.739896253486645 24 21.01830535312857 28.523753509284884 28.870498827081658 21.587442310504887 25 21.57963918042985 28.152129196684793 28.32064879292007 21.94758282996528 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5514.0 1 5514.0 2 5586.0 3 5658.0 4 5658.0 5 5658.0 6 8661.0 7 11664.0 8 11664.0 9 11664.0 10 16728.0 11 21792.0 12 21792.0 13 21792.0 14 34108.0 15 46424.0 16 46424.0 17 46424.0 18 69872.0 19 93320.0 20 93320.0 21 93320.0 22 127819.5 23 162319.0 24 162319.0 25 162319.0 26 208858.0 27 255397.0 28 255397.0 29 255397.0 30 302969.0 31 350541.0 32 350541.0 33 350541.0 34 389098.0 35 427655.0 36 427655.0 37 427655.0 38 445866.0 39 464077.0 40 464077.0 41 464077.0 42 458932.0 43 453787.0 44 453787.0 45 453787.0 46 468322.5 47 482858.0 48 482858.0 49 482858.0 50 443062.0 51 403266.0 52 403266.0 53 403266.0 54 351971.0 55 300676.0 56 300676.0 57 300676.0 58 257471.0 59 214266.0 60 214266.0 61 214266.0 62 180430.5 63 146595.0 64 146595.0 65 146595.0 66 113040.5 67 79486.0 68 79486.0 69 79486.0 70 59332.5 71 39179.0 72 39179.0 73 39179.0 74 27757.0 75 16335.0 76 16335.0 77 16335.0 78 11101.5 79 5868.0 80 5868.0 81 5868.0 82 3857.5 83 1847.0 84 1847.0 85 1847.0 86 1183.0 87 519.0 88 519.0 89 519.0 90 338.5 91 158.0 92 158.0 93 158.0 94 98.5 95 39.0 96 39.0 97 39.0 98 36.0 99 33.0 100 33.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10134678674535436 2 0.05489722062140144 3 0.05775488416059769 4 0.053117447715410805 5 0.0500843136080183 6 0.06860899216473779 7 0.06482384133650418 8 0.06176564000508364 9 0.06702975705097144 10 0.06590173196970978 11 0.061314429972578965 12 0.06231711893370045 13 0.06181577445313971 14 0.06532518581706491 15 0.06785697544389667 16 0.06274326174217709 17 0.06567612695345743 18 0.14436214317746618 19 0.07033863062267236 20 0.06412195906371913 21 0.06128936274855093 22 0.06725536206722378 23 0.06600200086582192 24 0.07675583997384987 25 0.07001275671030788 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3989273.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.10072384898243 #Duplication Level Percentage of deduplicated Percentage of total 1 81.62117678932192 53.952188671799426 2 12.24563105840611 16.188901538964494 3 3.239831300858255 6.42465582405963 4 1.1470608794575792 3.032862177239854 5 0.5271716999758136 1.7423215480549932 6 0.31508303459503817 1.2496329995559594 7 0.18349694081673076 0.8490496428441854 8 0.12512887913466905 0.6616887588170767 9 0.09019116726314141 0.5365531296790464 >10 0.4495651569187953 5.275051311555481 >50 0.02995945358900763 1.3726408781655999 >100 0.020905064703825523 2.6356352955985862 >500 0.0021881502443410737 1.02020761087206 >1k 0.0023033159248153647 2.9605431511201528 >5k 1.9194299373461276E-4 0.9221580226007936 >10k+ 1.1516579624076767E-4 1.1759094390727656 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 16641 0.4171436750505669 No Hit CCCATGTACTCTGCGTTGATACCAC 15724 0.3941570306168567 No Hit GTATCAACGCAGAGTACTTTTTTTT 13976 0.35033952301584775 No Hit GGTATCAACGCAGAGTACTTTTTTT 9460 0.2371359393052318 No Hit TATCAACGCAGAGTACTTTTTTTTT 8526 0.21372315206304504 No Hit GAGTACATGGGAAGCAGTGGTATCA 7347 0.18416889493398922 No Hit CATGTACTCTGCGTTGATACCACTG 5904 0.14799689066153157 No Hit ATTCCATTCCATTCCATTCCATTCC 5104 0.1279431114391018 No Hit GCGTTGATACCACTGCTTCCCATGT 4688 0.11751514624343834 No Hit GTATCAACGCAGAGTACATGGGAAG 4248 0.10648556767110198 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 7.520167208411156E-5 0.0 8 0.0 0.0 0.0 7.520167208411156E-5 0.0 9 0.0 2.506722402803719E-5 0.0 7.520167208411156E-5 0.0 10 0.0 2.506722402803719E-5 0.0 1.5040334416822313E-4 0.0 11 0.0 2.506722402803719E-5 0.0 1.5040334416822313E-4 0.0 12 0.0 2.506722402803719E-5 0.0 1.5040334416822313E-4 0.0 13 0.0 2.506722402803719E-5 0.0 1.5040334416822313E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTCGG 60 4.104064E-4 12.663173 7 TACACCG 115 7.10952E-8 11.562317 5 AAATCGC 180 3.929017E-10 10.0313015 17 TCTAGAC 285 0.0 9.997868 3 TATACTG 1010 0.0 9.967796 5 AATACCG 105 4.1199302E-5 9.949884 5 CGCCCTA 680 0.0 9.922479 16 TGTCCCG 710 0.0 9.898304 11 AGCACCG 155 4.0852683E-8 9.803991 5 AATCGCC 175 2.5538611E-9 9.77658 18 GTTCTAG 370 0.0 9.757736 1 GAAATCG 190 1.0477379E-9 9.503219 16 GCTACAC 240 1.8189894E-12 9.497974 3 ACCGCTC 90 0.0011171347 9.497378 8 GTCCCGC 740 0.0 9.374562 12 GTATTAG 500 0.0 9.310935 1 TCCCGCC 765 0.0 9.1926565 13 CCGTACA 560 0.0 9.163933 17 CCCGCCC 750 0.0 9.12309 14 CTAGGAC 230 4.1836756E-11 9.085019 3 >>END_MODULE