FastQCFastQC Report
Wed 25 May 2016
SRR1294600_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294600_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3989273
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT336390.843236349079143No Hit
GGTATCAACGCAGAGTACTTTTTTT243490.6103618378586775No Hit
TATCAACGCAGAGTACTTTTTTTTT223460.560152188130519No Hit
CCCATGTACTCTGCGTTGATACCAC180060.4513604358488376No Hit
GTACATGGGAAGCAGTGGTATCAAC161420.40463513026057624No Hit
ACGCAGAGTACTTTTTTTTTTTTTT128070.32103593812707226No Hit
GTACTTTTTTTTTTTTTTTTTTTTT83070.2082334300009049No Hit
GAGTACATGGGAAGCAGTGGTATCA77870.19519847350632558No Hit
GAGTACTTTTTTTTTTTTTTTTTTT67690.16968003944578372No Hit
CATGTACTCTGCGTTGATACCACTG66130.16576955249740993No Hit
GCGTTGATACCACTGCTTCCCATGT51520.1291463381924476No Hit
ATTCCATTCCATTCCATTCCATTCC49160.12323047332183082No Hit
ACGCAGAGTACATGGGAAGCAGTGG48780.1222779188087654No Hit
GCAGAGTACTTTTTTTTTTTTTTTT43780.10974430679474682No Hit
GTATCAACGCAGAGTACATGGGAAG43150.10816507168098047No Hit
GAATGGAATGGAATGGAATGGAATG42670.10696184492763469No Hit
TATCAACGCAGAGTACATGGGAAGC40200.1007702405927095No Hit
GGTATCAACGCAGAGTACATGGGAA40180.10072010614465342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCC1657.2759576E-1211.49418518
CTTACGC600.0058867811.081213
GTCCGGA700.00148390910.8644741
CGACCGC750.002601015810.15574510
CGCTCGC750.002689474310.11488216
CCTATAC3450.09.6358343
TCTAGAC4250.09.6099223
CCCCCGG5250.09.57301318
ACCGTTC900.0010917769.521258
CAATCCG1606.422124E-89.5210110
GACCGCC800.00456614379.48781611
ACGATCT1401.0864278E-69.48270318
TAGACAG5850.09.4398715
CCGTACA5850.09.40165317
CCCCGGT5350.09.39419719
CGCCCTA4950.09.38691816
GTCCTAG3150.09.3555191
ACCGGGG3400.09.20379915
CCCCGTA6000.09.16661315
TAGTACC2950.09.0371194
Produced by FastQC (version 0.11.5)